BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060219.seq (652 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 53 4e-08 SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr 2... 29 0.77 SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pom... 26 5.4 SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 5.4 SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomy... 26 5.4 SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual 26 5.4 SPAC26H5.07c |||seven transmembrane receptor-like protein|Schizo... 25 9.5 SPAC823.16c |mug179||WD repeat protein Mug179|Schizosaccharomyce... 25 9.5 SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces po... 25 9.5 SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 25 9.5 >SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 685 Score = 52.8 bits (121), Expect = 4e-08 Identities = 53/199 (26%), Positives = 79/199 (39%), Gaps = 14/199 (7%) Frame = +1 Query: 52 NKLRIDSIVATNASKSGHPTSCASMAEIMSVLFFHTMRYKISAPRDASADRFILSKGHAA 231 N +R ++ T +KSGHP + +A VLF M++ + P+ + DRFILS GHA Sbjct: 15 NTIRTLAVDTTAHAKSGHPGAPMGLAPAAHVLFSRIMKFNPAHPKWLNRDRFILSNGHAC 74 Query: 232 PILYAAWARPG----CSRWTS*RTCASWTRTWRATPPRDSTSWTXAPAPWARGSPWPAGM 399 + Y G R S T T D T A P +G G+ Sbjct: 75 VLQYIMCHLLGYKLTIEDLKQFRQVGSKTPGHPETHNPDLNIETGA-GPLGQGIASAVGL 133 Query: 400 AYVGKYFDQAPYR----------VYCLVGDGEAAEGSIWESLHFASPTSWTTWSVIFDVN 549 A +GK A Y +C +GDG EG E+ A + ++D N Sbjct: 134 A-IGKAHSAAVYNKPGFDLFSNYTFCFLGDGCLQEGVSSEACSLAGHLKLSNLIAVWDNN 192 Query: 550 RLXQSEPTXLQHQLEVYRR 606 ++ T + +V +R Sbjct: 193 KITIDGATSMSFDEDVEKR 211 Score = 35.1 bits (77), Expect = 0.009 Identities = 18/42 (42%), Positives = 26/42 (61%), Gaps = 3/42 (7%) Frame = +3 Query: 273 LDELKNLRKLDSDLEGHP---TPRLNFVDVGTGSLGQGLAVA 389 +++LK R++ S GHP P LN ++ G G LGQG+A A Sbjct: 90 IEDLKQFRQVGSKTPGHPETHNPDLN-IETGAGPLGQGIASA 130 >SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr 2|||Manual Length = 825 Score = 28.7 bits (61), Expect = 0.77 Identities = 18/58 (31%), Positives = 25/58 (43%), Gaps = 3/58 (5%) Frame = +1 Query: 409 GKYFDQAPYRVYCLVGDGEAAEG---SIWESLHFASPTSWTTWSVIFDVNRLXQSEPT 573 G D+ V C+VGDGEA G + W + F P + ++N SE T Sbjct: 200 GAVLDRPDLIVTCVVGDGEAETGPTATSWHAHKFLDPAESGAVIPVLELNGYKISERT 257 >SPAC25B8.05 |||pseudouridylate synthase |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 25.8 bits (54), Expect = 5.4 Identities = 13/43 (30%), Positives = 21/43 (48%) Frame = +3 Query: 333 RLNFVDVGTGSLGQGLAVAGRHGLRREVLRPGALQGVLPGGRR 461 R D G ++GQ +++ RH RR ++ L +LP R Sbjct: 144 RCGRTDKGVSAMGQVISLNVRHSERRPIIYCDVLNRLLPADIR 186 >SPAC688.07c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1038 Score = 25.8 bits (54), Expect = 5.4 Identities = 13/26 (50%), Positives = 17/26 (65%), Gaps = 1/26 (3%) Frame = -1 Query: 391 PATASPWPR-EPVPTSTKLSRGVGWP 317 P A P PR EPV T+TKL++ + P Sbjct: 943 PKEAPPPPRSEPVSTTTKLAKRITQP 968 >SPBP35G2.06c |nup131|Nup133a|nucleoporin Nup131|Schizosaccharomyces pombe|chr 2|||Manual Length = 1142 Score = 25.8 bits (54), Expect = 5.4 Identities = 15/48 (31%), Positives = 22/48 (45%) Frame = +1 Query: 457 DGEAAEGSIWESLHFASPTSWTTWSVIFDVNRLXQSEPTXLQHQLEVY 600 +GEA E + + S S W V V RL Q+E T L ++ + Sbjct: 946 EGEAVEAIVTSNCVNLSENSIRAWLVSSAVERLIQNEYTTLCEMVDFF 993 >SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual Length = 706 Score = 25.8 bits (54), Expect = 5.4 Identities = 11/19 (57%), Positives = 14/19 (73%) Frame = -1 Query: 433 RAPGRSTSRRRPCRPATAS 377 R PGRS SRRR RP+ ++ Sbjct: 205 RRPGRSRSRRRSRRPSPSA 223 >SPAC26H5.07c |||seven transmembrane receptor-like protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 505 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/28 (42%), Positives = 16/28 (57%) Frame = +2 Query: 44 ISPTN*ESTVLLLQTPQSLVTRHHVRQW 127 ISP+N ES V+L P L+T + W Sbjct: 309 ISPSNKESLVILFAAPVFLITLFAMFLW 336 >SPAC823.16c |mug179||WD repeat protein Mug179|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = -3 Query: 476 PSAASPSPTRQYTL*GAWSKY 414 PS + SPT T+ +WSKY Sbjct: 245 PSDPNSSPTSSVTVSSSWSKY 265 >SPCP1E11.05c |||sterol O-acyltransferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 472 Score = 25.0 bits (52), Expect = 9.5 Identities = 12/27 (44%), Positives = 14/27 (51%) Frame = +1 Query: 160 MRYKISAPRDASADRFILSKGHAAPIL 240 +R I AP DASA F + GH L Sbjct: 295 IRTIIEAPEDASATYFAIRLGHTVAFL 321 >SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 713 Score = 25.0 bits (52), Expect = 9.5 Identities = 18/65 (27%), Positives = 29/65 (44%) Frame = -3 Query: 650 SVHPPAS*DRTLSGARRYTSS*CCRXVGSDCXRRLTSKMTDQVVQLVGLAKCSDSQMLPS 471 S+ PPAS + S T S + ++ LTS T + + L + + LPS Sbjct: 252 SIEPPAS-SASTSPRNTPTPSNNGTSINANVTSSLTSNSTGKTSKTTDLLIAASKKSLPS 310 Query: 470 AASPS 456 ++PS Sbjct: 311 NSTPS 315 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,528,828 Number of Sequences: 5004 Number of extensions: 50106 Number of successful extensions: 162 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 152 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 161 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 293780908 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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