Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060212.seq
         (658 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si...    71   9e-13
At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co...    40   0.001
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si...    38   0.006
At2g16920.1 68415.m01949 ubiquitin-conjugating enzyme family pro...    31   0.51 
At5g04540.1 68418.m00454 expressed protein                             29   3.6  
At5g57035.1 68418.m07119 protein kinase family protein contains ...    27   8.3  
At5g38530.1 68418.m04659 tryptophan synthase-related low similar...    27   8.3  
At1g09450.1 68414.m01057 haspin-related similar to haspin (GI:92...    27   8.3  

>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase precursor SP:P43077 from
           [Candida albicans]
          Length = 580

 Score = 70.5 bits (165), Expect = 9e-13
 Identities = 34/76 (44%), Positives = 46/76 (60%), Gaps = 5/76 (6%)
 Frame = +1

Query: 256 ALWSEQSDPATLDGRLWPRAAAFAERMWA-----EPSTAWQDAEHRMLHVRERLVRMGIQ 420
           ALWSEQ+D   LD RLWPRA+A AE +W+            +A  R+   R R+V+ GI 
Sbjct: 495 ALWSEQADSTVLDSRLWPRASALAESLWSGNRDERGVKRCGEAVDRLNLWRYRMVKRGIG 554

Query: 421 AESLEPEWCYQNQGLC 468
           AE ++P WC +N G+C
Sbjct: 555 AEPIQPLWCLKNPGMC 570



 Score = 56.0 bits (129), Expect = 2e-08
 Identities = 29/93 (31%), Positives = 50/93 (53%), Gaps = 12/93 (12%)
 Frame = +2

Query: 11  KDEYIIQVWTTGADPQIQGLLQKGYRLIMSNYDALYFDCGFGAWVGS----------GNN 160
           K+  I+Q W  G +   + ++  GYR+I+S+ +  Y DCG G ++G+          G +
Sbjct: 402 KEHTILQTWNNGPE-NTKRIVAAGYRVIVSSSEFYYLDCGHGGFLGNDSIYDQKESGGGS 460

Query: 161 WCSPYIGWQKVYGNSPAVMALSYRDQ--ILGGE 253
           WC+P+  WQ +Y    A   L+  ++  +LGGE
Sbjct: 461 WCAPFKTWQSIYNYDIADGLLNEEERKLVLGGE 493


>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
           contains Pfam PF00728: Glycosyl hydrolase family 20,
           catalytic domain; contains Pfam PF02838: Glycosyl
           hydrolase family 20, domain 2; similar to
           Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
           (N-acetyl-beta- glucosaminidase)
           (Beta-N-acetylhexosaminidase) (Hexosaminidase B)
           (Swiss-Prot:P07686) [Homo sapiens]
          Length = 535

 Score = 40.3 bits (90), Expect = 0.001
 Identities = 21/61 (34%), Positives = 33/61 (54%), Gaps = 3/61 (4%)
 Frame = +1

Query: 259 LWSEQSDPATLDGRLWPRAAAFAERMW---AEPSTAWQDAEHRMLHVRERLVRMGIQAES 429
           +W E  D + ++  +WPRAAA AER+W   A+ +    +   R+ H R  L + G+ A  
Sbjct: 458 MWGEHIDASDIEQTIWPRAAAAAERLWTPYAKLAKNPNNVTTRLAHFRCLLNQRGVAAAP 517

Query: 430 L 432
           L
Sbjct: 518 L 518


>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase A SP:P13723 from
           [Dictyostelium discoideum]
          Length = 541

 Score = 37.9 bits (84), Expect = 0.006
 Identities = 14/28 (50%), Positives = 20/28 (71%)
 Frame = +1

Query: 259 LWSEQSDPATLDGRLWPRAAAFAERMWA 342
           +W E +D + +   +WPRAAA AERMW+
Sbjct: 461 MWGETADTSVVLQTIWPRAAAAAERMWS 488


>At2g16920.1 68415.m01949 ubiquitin-conjugating enzyme family
           protein low similarity to ubiquitin-conjugating
           BIR-domain enzyme APOLLON [Homo sapiens] GI:8489831,
           ubiquitin-conjugating enzyme [Mus musculus] GI:3319990;
           contains Pfam profile PF00179: Ubiquitin-conjugating
           enzyme
          Length = 1102

 Score = 31.5 bits (68), Expect = 0.51
 Identities = 13/34 (38%), Positives = 23/34 (67%)
 Frame = +2

Query: 230 RDQILGGEERCGLSSQTLPRWTADCGPERLPSPS 331
           +D++ G  E  G++++ LP+ T+D  P+R PS S
Sbjct: 379 QDEVSGKVEPAGITAEALPKVTSDDPPQRNPSVS 412


>At5g04540.1 68418.m00454 expressed protein
          Length = 833

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 12/28 (42%), Positives = 15/28 (53%)
 Frame = +1

Query: 277 DPATLDGRLWPRAAAFAERMWAEPSTAW 360
           DP+  DG L P AAA A  +W +    W
Sbjct: 623 DPSRYDGPLLPPAAALAPTLWPQFHLRW 650


>At5g57035.1 68418.m07119 protein kinase family protein contains
           Pfam profile: PF00069 Eukaryotic protein kinase domain
          Length = 786

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 15/46 (32%), Positives = 21/46 (45%)
 Frame = -3

Query: 395 SLTWSIRCSASCHAVEGSAHMRSAKAAALGHSRPSSVAGSDCSDHS 258
           S T S +   S  ++  S  MR  +  +L HS P+S   SD    S
Sbjct: 206 SRTRSDKTGLSASSITSSGRMRIGRPGSLPHSHPTSRVYSDAQSSS 251


>At5g38530.1 68418.m04659 tryptophan synthase-related low similarity
           to tryptophan synthases, beta subunit, from Lactococcus
           lactis [SP|Q01998], Pyrococcus kodakaraensis
           [SP|Q9YGB0], Thermus thermophilus [SP|P16609]; contains
           Pfam pyridoxal-phosphate dependent enzyme domain PF00291
          Length = 506

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 13/31 (41%), Positives = 16/31 (51%)
 Frame = -2

Query: 228 YESAITAGLLPYTFCHPMYGEHQLLPDPTHA 136
           Y+   TAGL P    H +   H  +PDP HA
Sbjct: 361 YDFGDTAGLTPLMKMHTL--GHDFIPDPIHA 389


>At1g09450.1 68414.m01057 haspin-related similar to haspin
           (GI:9229937) {Mus musculus} and haploid germ
           cell-specific nuclear protein kinase (GI:13561418) {Mus
           musculus}
          Length = 599

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 13/33 (39%), Positives = 17/33 (51%)
 Frame = -2

Query: 435 LKRFSLNSHSYQTFSDMEHPVLGVLPRGGRFGP 337
           LKR SL S    T  D + P+L +L   G+  P
Sbjct: 242 LKRLSLTSDLIPTHQDFDQPILDLLSACGQMRP 274


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,091,526
Number of Sequences: 28952
Number of extensions: 375400
Number of successful extensions: 1011
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 951
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1009
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)