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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060206.seq
         (677 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g13950.1 68414.m01639 eukaryotic translation initiation facto...   105   3e-23
At1g26630.1 68414.m03243 eukaryotic translation initiation facto...   103   8e-23
At1g69410.1 68414.m07972 eukaryotic translation initiation facto...   103   1e-22
At5g14230.1 68418.m01663 ankyrin repeat family protein contains ...    29   2.8  
At5g41460.1 68418.m05035 fringe-related protein strong similarit...    29   3.7  
At5g03140.1 68418.m00262 lectin protein kinase family protein co...    29   3.7  
At3g06450.1 68416.m00746 anion exchange family protein similar t...    28   4.9  
At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro...    28   6.5  
At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein...    28   6.5  
At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-...    27   8.6  
At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila...    27   8.6  
At3g44460.1 68416.m04779 basic leucine zipper transcription fact...    27   8.6  
At1g20860.1 68414.m02613 phosphate transporter family protein si...    27   8.6  

>At1g13950.1 68414.m01639 eukaryotic translation initiation factor
           5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic
           translation initiation factor 5A-1 (eIF-5A 1)
           {Arabidopsis thaliana}
          Length = 158

 Score =  105 bits (252), Expect = 3e-23
 Identities = 47/86 (54%), Positives = 66/86 (76%), Gaps = 1/86 (1%)
 Frame = +2

Query: 257 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 433
           KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG  ++DLK+P+ D L  Q++
Sbjct: 68  KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127

Query: 434 TDFDSGKELLCTVLKSCGEECVIAVK 511
           + FD GK+L+ +V+ + GEE + A+K
Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153



 Score =  104 bits (250), Expect = 5e-23
 Identities = 43/72 (59%), Positives = 56/72 (77%)
 Frame = +3

Query: 66  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 245
           E+ HFE+ D+GAS T+P Q   +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 246 IFNVKSMKISVP 281
           IF  K ++  VP
Sbjct: 64  IFTSKKLEDIVP 75


>At1g26630.1 68414.m03243 eukaryotic translation initiation factor
           5A, putative / eIF-5A, putative strong similariy to
           SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1
           (eIF-5A 1) {Lycopersicon esculentum}
          Length = 159

 Score =  103 bits (248), Expect = 8e-23
 Identities = 46/87 (52%), Positives = 65/87 (74%), Gaps = 1/87 (1%)
 Frame = +2

Query: 257 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 433
           KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G  ++DLK+P D  L  Q+R
Sbjct: 68  KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127

Query: 434 TDFDSGKELLCTVLKSCGEECVIAVKQ 514
             FD GK+++ +V+ S GEE + AVK+
Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVKE 154



 Score = 96.7 bits (230), Expect = 1e-20
 Identities = 40/72 (55%), Positives = 52/72 (72%)
 Frame = +3

Query: 66  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 245
           ++ HFE  +SGAS T+P     +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 246 IFNVKSMKISVP 281
           IF  K ++  VP
Sbjct: 64  IFTAKKLEDIVP 75


>At1g69410.1 68414.m07972 eukaryotic translation initiation factor
           5A, putative / eIF-5A, putative strong similarity to
           eukaryotic initiation factor 5A (2) (Nicotiana
           plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic
           translation initiation factor 5A-1 (eIF-5A 1)
           {Lycopersicon esculentum}
          Length = 158

 Score =  103 bits (246), Expect = 1e-22
 Identities = 46/87 (52%), Positives = 66/87 (75%), Gaps = 1/87 (1%)
 Frame = +2

Query: 257 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 433
           KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG  ++DLK+P D  L TQL+
Sbjct: 68  KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127

Query: 434 TDFDSGKELLCTVLKSCGEECVIAVKQ 514
             F+ GK+++ +V+ + GEE + A+K+
Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALKE 154



 Score =  101 bits (241), Expect = 6e-22
 Identities = 42/72 (58%), Positives = 55/72 (76%)
 Frame = +3

Query: 66  EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 245
           ++ HFE+ D+GAS T+P Q   +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID
Sbjct: 4   DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63

Query: 246 IFNVKSMKISVP 281
           IF  K ++  VP
Sbjct: 64  IFTSKKLEDIVP 75


>At5g14230.1 68418.m01663 ankyrin repeat family protein contains
           ankyrin repeats, Pfam:PF00023
          Length = 591

 Score = 29.1 bits (62), Expect = 2.8
 Identities = 15/34 (44%), Positives = 16/34 (47%)
 Frame = +2

Query: 395 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 496
           L IPDGD  T L      G   +C  L SCG  C
Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445


>At5g41460.1 68418.m05035 fringe-related protein strong similarity
           to unknown protein (pir||T13026) similarity to predicted
           proteins + similar to hypothetical protein GB:AAC23643
           [Arabidopsis thaliana] + weak similarity to Fringe
           [Schistocerca gregaria](GI:6573138);Fringe encodes an
           extracellular protein that regulates Notch signalling.
          Length = 524

 Score = 28.7 bits (61), Expect = 3.7
 Identities = 11/29 (37%), Positives = 16/29 (55%)
 Frame = +1

Query: 31  VVKFKTQQWVTSKTHTSRPETPGPQPPSP 117
           ++ F T Q    K++ S P +P P PP P
Sbjct: 80  LILFHTNQTAVIKSYASPPPSPPPPPPPP 108


>At5g03140.1 68418.m00262 lectin protein kinase family protein
           contains Pfam domains, PF00138: Legume lectins alpha
           domain, PF00139: Legume lectins beta domain and PF00069:
           Protein kinase domain
          Length = 711

 Score = 28.7 bits (61), Expect = 3.7
 Identities = 13/36 (36%), Positives = 21/36 (58%)
 Frame = -2

Query: 166 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 59
           +P  +T+P +R     +   A+ PE P++K  SSMS
Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666


>At3g06450.1 68416.m00746 anion exchange family protein similar to
           putative Anion exchanger family members: GB:AAD39673,
           GB:AAD55295 [Arabidopsis thaliana]
          Length = 732

 Score = 28.3 bits (60), Expect = 4.9
 Identities = 11/21 (52%), Positives = 12/21 (57%)
 Frame = -1

Query: 668 LRAPPLYYYSFFKLNLYXILC 606
           LR PP Y+Y  F L    ILC
Sbjct: 328 LRKPPAYHYDLFLLGFLTILC 348


>At3g53330.1 68416.m05884 plastocyanin-like domain-containing
           protein similar to mavicyanin SP:P80728 from [Cucurbita
           pepo]
          Length = 310

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%)
 Frame = +1

Query: 64  SKTHT-SRPETPGPQPPS 114
           SKTH  SRP TP P PPS
Sbjct: 127 SKTHERSRPITPSPPPPS 144


>At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein
           contains INTERPRO domain, IPR001878: Zn-finger CCHC type
          Length = 353

 Score = 27.9 bits (59), Expect = 6.5
 Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%)
 Frame = +1

Query: 67  KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 192
           KT T       P PP    PCN  PC    S   +  + R   CP
Sbjct: 22  KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66


>At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box
           RNA helicase [Chlamydomonas reinhardtii] GI:12044832;
           contains Pfam profiles PF04408: Helicase associated
           domain (HA2), PF00271: Helicase conserved C-terminal
           domain
          Length = 1226

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
 Frame = +2

Query: 308 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 457
           V+R+  +++      Y  L ADN    +   +  G + GT+++T+FDS +E
Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400


>At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to
            SP|P35207 Antiviral protein SKI2 {Saccharomyces
            cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH
            box helicase, PF00271: Helicase conserved C-terminal
            domain
          Length = 1347

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%)
 Frame = +2

Query: 338  ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 472
            +SD+  L + A  G  D+ +++   D DL  Q++     + +SG+EL+CTV
Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191


>At3g44460.1 68416.m04779 basic leucine zipper transcription factor
           (BZIP67) identical to basic leucine zipper transcription
           factor GI:18656053 from [Arabidopsis thaliana];
           identical to cDNA basic leucine zipper transcription
           factor (atbzip67 gene) GI:18656052
          Length = 331

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 2/50 (4%)
 Frame = +3

Query: 141 NGFVMLKGRPCKIV--EMSTSKTGKHGHAKVHLVGIDIFNVKSMKISVPP 284
           N  V  + RP   V  E S+  TG +G +  +L G+D F +K   I  PP
Sbjct: 195 NRSVYSENRPFYSVLGESSSCMTG-NGRSNQYLTGLDAFRIKKRIIDGPP 243


>At1g20860.1 68414.m02613 phosphate transporter family protein
           similar to phosphate transporter [Catharanthus roseus]
           GI:2208908, inorganic phosphate transporter 1 [Solanum
           tuberosum] GI:1420871; contains Pfam profile PF00083:
           major facilitator superfamily protein
          Length = 534

 Score = 27.5 bits (58), Expect = 8.6
 Identities = 17/45 (37%), Positives = 20/45 (44%)
 Frame = +1

Query: 43  KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 177
           K  Q V S++H S   T  P PP P    P  K  S C   +H R
Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,620,847
Number of Sequences: 28952
Number of extensions: 319476
Number of successful extensions: 986
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 903
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 972
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1428369392
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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