BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060206.seq (677 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g13950.1 68414.m01639 eukaryotic translation initiation facto... 105 3e-23 At1g26630.1 68414.m03243 eukaryotic translation initiation facto... 103 8e-23 At1g69410.1 68414.m07972 eukaryotic translation initiation facto... 103 1e-22 At5g14230.1 68418.m01663 ankyrin repeat family protein contains ... 29 2.8 At5g41460.1 68418.m05035 fringe-related protein strong similarit... 29 3.7 At5g03140.1 68418.m00262 lectin protein kinase family protein co... 29 3.7 At3g06450.1 68416.m00746 anion exchange family protein similar t... 28 4.9 At3g53330.1 68416.m05884 plastocyanin-like domain-containing pro... 28 6.5 At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein... 28 6.5 At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-... 27 8.6 At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative simila... 27 8.6 At3g44460.1 68416.m04779 basic leucine zipper transcription fact... 27 8.6 At1g20860.1 68414.m02613 phosphate transporter family protein si... 27 8.6 >At1g13950.1 68414.m01639 eukaryotic translation initiation factor 5A-1 / eIF-5A 1 identical to SP|Q9XI91 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Arabidopsis thaliana} Length = 158 Score = 105 bits (252), Expect = 3e-23 Identities = 47/86 (54%), Positives = 66/86 (76%), Gaps = 1/86 (1%) Frame = +2 Query: 257 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGD-LGTQLR 433 KK EDI PS+HN DVPHV R DYQL DIS+DGY++L+ DNG ++DLK+P+ D L Q++ Sbjct: 68 KKLEDIVPSSHNCDVPHVNRTDYQLIDISEDGYVSLLTDNGSTKDDLKLPNDDTLLQQIK 127 Query: 434 TDFDSGKELLCTVLKSCGEECVIAVK 511 + FD GK+L+ +V+ + GEE + A+K Sbjct: 128 SGFDDGKDLVVSVMSAMGEEQINALK 153 Score = 104 bits (250), Expect = 5e-23 Identities = 43/72 (59%), Positives = 56/72 (77%) Frame = +3 Query: 66 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 245 E+ HFE+ D+GAS T+P Q +RKNG++++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 EEHHFESSDAGASKTYPQQAGTIRKNGYIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 246 IFNVKSMKISVP 281 IF K ++ VP Sbjct: 64 IFTSKKLEDIVP 75 >At1g26630.1 68414.m03243 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similariy to SP|Q9AXQ6 Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 159 Score = 103 bits (248), Expect = 8e-23 Identities = 46/87 (52%), Positives = 65/87 (74%), Gaps = 1/87 (1%) Frame = +2 Query: 257 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 433 KK EDI PS+HN DVPHV R DYQL DI++DG+++L+ D+G ++DLK+P D L Q+R Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDITEDGFVSLLTDSGGTKDDLKLPTDDGLTAQMR 127 Query: 434 TDFDSGKELLCTVLKSCGEECVIAVKQ 514 FD GK+++ +V+ S GEE + AVK+ Sbjct: 128 LGFDEGKDIVVSVMSSMGEEQICAVKE 154 Score = 96.7 bits (230), Expect = 1e-20 Identities = 40/72 (55%), Positives = 52/72 (72%) Frame = +3 Query: 66 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 245 ++ HFE +SGAS T+P +RK G +++K RPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFEASESGASKTYPQSAGNIRKGGHIVIKNRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 246 IFNVKSMKISVP 281 IF K ++ VP Sbjct: 64 IFTAKKLEDIVP 75 >At1g69410.1 68414.m07972 eukaryotic translation initiation factor 5A, putative / eIF-5A, putative strong similarity to eukaryotic initiation factor 5A (2) (Nicotiana plumbaginifolia) GI:19702, SP|Q9AXQ6| Eukaryotic translation initiation factor 5A-1 (eIF-5A 1) {Lycopersicon esculentum} Length = 158 Score = 103 bits (246), Expect = 1e-22 Identities = 46/87 (52%), Positives = 66/87 (75%), Gaps = 1/87 (1%) Frame = +2 Query: 257 KKYEDICPSTHNMDVPHVKREDYQLTDISDDGYLTLMADNGDLREDLKIP-DGDLGTQLR 433 KK EDI PS+HN DVPHV R DYQL DIS+DG+++L+ DNG ++DLK+P D L TQL+ Sbjct: 68 KKLEDIVPSSHNCDVPHVNRVDYQLIDISEDGFVSLLTDNGSTKDDLKLPTDEALLTQLK 127 Query: 434 TDFDSGKELLCTVLKSCGEECVIAVKQ 514 F+ GK+++ +V+ + GEE + A+K+ Sbjct: 128 NGFEEGKDIVVSVMSAMGEEQMCALKE 154 Score = 101 bits (241), Expect = 6e-22 Identities = 42/72 (58%), Positives = 55/72 (76%) Frame = +3 Query: 66 EDTHFETGDSGASATFPMQCSALRKNGFVMLKGRPCKIVEMSTSKTGKHGHAKVHLVGID 245 ++ HFE+ D+GAS T+P Q +RK G +++KGRPCK+VE+STSKTGKHGHAK H V ID Sbjct: 4 DEHHFESSDAGASKTYPQQAGNIRKGGHIVIKGRPCKVVEVSTSKTGKHGHAKCHFVAID 63 Query: 246 IFNVKSMKISVP 281 IF K ++ VP Sbjct: 64 IFTSKKLEDIVP 75 >At5g14230.1 68418.m01663 ankyrin repeat family protein contains ankyrin repeats, Pfam:PF00023 Length = 591 Score = 29.1 bits (62), Expect = 2.8 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = +2 Query: 395 LKIPDGDLGTQLRTDFDSGKELLCTVLKSCGEEC 496 L IPDGD T L G +C L SCG C Sbjct: 412 LDIPDGDGYTPLMLAAREGHGHMCEYLISCGANC 445 >At5g41460.1 68418.m05035 fringe-related protein strong similarity to unknown protein (pir||T13026) similarity to predicted proteins + similar to hypothetical protein GB:AAC23643 [Arabidopsis thaliana] + weak similarity to Fringe [Schistocerca gregaria](GI:6573138);Fringe encodes an extracellular protein that regulates Notch signalling. Length = 524 Score = 28.7 bits (61), Expect = 3.7 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +1 Query: 31 VVKFKTQQWVTSKTHTSRPETPGPQPPSP 117 ++ F T Q K++ S P +P P PP P Sbjct: 80 LILFHTNQTAVIKSYASPPPSPPPPPPPP 108 >At5g03140.1 68418.m00262 lectin protein kinase family protein contains Pfam domains, PF00138: Legume lectins alpha domain, PF00139: Legume lectins beta domain and PF00069: Protein kinase domain Length = 711 Score = 28.7 bits (61), Expect = 3.7 Identities = 13/36 (36%), Positives = 21/36 (58%) Frame = -2 Query: 166 RPFSITKPFLRRAEHCMGKVAEAPESPVSKCVSSMS 59 +P +T+P +R + A+ PE P++K SSMS Sbjct: 631 QPDPVTRPTMRSVVQILVGEADVPEVPIAKPSSSMS 666 >At3g06450.1 68416.m00746 anion exchange family protein similar to putative Anion exchanger family members: GB:AAD39673, GB:AAD55295 [Arabidopsis thaliana] Length = 732 Score = 28.3 bits (60), Expect = 4.9 Identities = 11/21 (52%), Positives = 12/21 (57%) Frame = -1 Query: 668 LRAPPLYYYSFFKLNLYXILC 606 LR PP Y+Y F L ILC Sbjct: 328 LRKPPAYHYDLFLLGFLTILC 348 >At3g53330.1 68416.m05884 plastocyanin-like domain-containing protein similar to mavicyanin SP:P80728 from [Cucurbita pepo] Length = 310 Score = 27.9 bits (59), Expect = 6.5 Identities = 13/18 (72%), Positives = 13/18 (72%), Gaps = 1/18 (5%) Frame = +1 Query: 64 SKTHT-SRPETPGPQPPS 114 SKTH SRP TP P PPS Sbjct: 127 SKTHERSRPITPSPPPPS 144 >At1g54930.1 68414.m06273 zinc knuckle (CCHC-type) family protein contains INTERPRO domain, IPR001878: Zn-finger CCHC type Length = 353 Score = 27.9 bits (59), Expect = 6.5 Identities = 15/45 (33%), Positives = 17/45 (37%), Gaps = 3/45 (6%) Frame = +1 Query: 67 KTHTSRPETPGPQPPS---PCNVRPCVKTVSLC*RVVHARLLKCP 192 KT T P PP PCN PC S + + R CP Sbjct: 22 KTTTKPTAAAAPSPPDIHCPCNAGPCNTVTSKTEKNPNRRFYTCP 66 >At5g13010.1 68418.m01491 RNA helicase, putative similar to DEAH-box RNA helicase [Chlamydomonas reinhardtii] GI:12044832; contains Pfam profiles PF04408: Helicase associated domain (HA2), PF00271: Helicase conserved C-terminal domain Length = 1226 Score = 27.5 bits (58), Expect = 8.6 Identities = 15/51 (29%), Positives = 28/51 (54%), Gaps = 1/51 (1%) Frame = +2 Query: 308 VKREDYQLTDISDDGYLTLMADNGDLREDLKIPDGDL-GTQLRTDFDSGKE 457 V+R+ +++ Y L ADN + + G + GT+++T+FDS +E Sbjct: 350 VRRDGSKMSLAQSKKYSQLNADNAQWEDRQLLRSGAVRGTEVQTEFDSEEE 400 >At3g46960.1 68416.m05099 DEAD/DEAH box helicase, putative similar to SP|P35207 Antiviral protein SKI2 {Saccharomyces cerevisiae}; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1347 Score = 27.5 bits (58), Expect = 8.6 Identities = 17/51 (33%), Positives = 30/51 (58%), Gaps = 6/51 (11%) Frame = +2 Query: 338 ISDDGYLTLMADNG--DLREDLKIPDGDLGTQLR----TDFDSGKELLCTV 472 +SD+ L + A G D+ +++ D DL Q++ + +SG+EL+CTV Sbjct: 1141 MSDEALLQMPAFQGRIDVLKNIGCIDDDLVVQIKGRVACEMNSGEELICTV 1191 >At3g44460.1 68416.m04779 basic leucine zipper transcription factor (BZIP67) identical to basic leucine zipper transcription factor GI:18656053 from [Arabidopsis thaliana]; identical to cDNA basic leucine zipper transcription factor (atbzip67 gene) GI:18656052 Length = 331 Score = 27.5 bits (58), Expect = 8.6 Identities = 18/50 (36%), Positives = 25/50 (50%), Gaps = 2/50 (4%) Frame = +3 Query: 141 NGFVMLKGRPCKIV--EMSTSKTGKHGHAKVHLVGIDIFNVKSMKISVPP 284 N V + RP V E S+ TG +G + +L G+D F +K I PP Sbjct: 195 NRSVYSENRPFYSVLGESSSCMTG-NGRSNQYLTGLDAFRIKKRIIDGPP 243 >At1g20860.1 68414.m02613 phosphate transporter family protein similar to phosphate transporter [Catharanthus roseus] GI:2208908, inorganic phosphate transporter 1 [Solanum tuberosum] GI:1420871; contains Pfam profile PF00083: major facilitator superfamily protein Length = 534 Score = 27.5 bits (58), Expect = 8.6 Identities = 17/45 (37%), Positives = 20/45 (44%) Frame = +1 Query: 43 KTQQWVTSKTHTSRPETPGPQPPSPCNVRPCVKTVSLC*RVVHAR 177 K Q V S++H S T P PP P P K S C +H R Sbjct: 243 KDMQRVMSRSHISDEATTDPPPPPP---PPSYKLFSRCFFRLHGR 284 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,620,847 Number of Sequences: 28952 Number of extensions: 319476 Number of successful extensions: 986 Number of sequences better than 10.0: 13 Number of HSP's better than 10.0 without gapping: 903 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 972 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1428369392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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