BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060205.seq (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces p... 27 1.8 SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe... 27 1.8 SPAC24H6.04 |hxk1||hexokinase 1 |Schizosaccharomyces pombe|chr 1... 26 5.5 SPAC1399.03 |fur4||uracil permease|Schizosaccharomyces pombe|chr... 25 7.2 SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosa... 25 7.2 SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 25 9.5 SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizos... 25 9.5 SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc... 25 9.5 >SPAC22H12.05c |||fasciclin domain protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 728 Score = 27.5 bits (58), Expect = 1.8 Identities = 9/27 (33%), Positives = 19/27 (70%) Frame = +2 Query: 317 LITKSGVIQLIMKSKLPYAIELQEWLL 397 L+ K GV+ L+ K KLP+++ ++ ++ Sbjct: 542 LLVKDGVVHLVDKVKLPFSVSQKDMII 568 >SPCC553.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 349 Score = 27.5 bits (58), Expect = 1.8 Identities = 16/43 (37%), Positives = 23/43 (53%) Frame = +1 Query: 202 STSTASTNRRLSMPTKSRPCSR*RGKAGRPAVFAPTHSAHYQI 330 S+S+ S++R S T R S + + RP VF T +HY I Sbjct: 163 SSSSKSSSRSSSRTTSHRTTSH-KSSSYRPTVFPYTTISHYNI 204 >SPAC24H6.04 |hxk1||hexokinase 1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 484 Score = 25.8 bits (54), Expect = 5.5 Identities = 8/21 (38%), Positives = 16/21 (76%) Frame = +2 Query: 479 IDDLTQKLTVPTQIWRKPTDR 541 +D+L ++ T+PT++ + TDR Sbjct: 31 LDELEEQFTIPTELLHRVTDR 51 >SPAC1399.03 |fur4||uracil permease|Schizosaccharomyces pombe|chr 1|||Manual Length = 581 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/47 (29%), Positives = 20/47 (42%) Frame = -2 Query: 343 LNHARFGNEHCVWVQIQRVALLCHAIWSMVWIWSACSNVDLYLPSTW 203 L+ + FG +W + R L C +W V W V L + S W Sbjct: 123 LSRSSFGTWGSLWPILNRSVLAC--VWYGVQAWIGGECVVLMIRSIW 167 >SPAC22F3.10c |gcs1|apd1|glutamate-cysteine ligase Gcs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 669 Score = 25.4 bits (53), Expect = 7.2 Identities = 14/42 (33%), Positives = 20/42 (47%) Frame = +3 Query: 120 GDEQPVRFVAKDIASSLKYVNCERAIRVHVDGKYKSTFEHAD 245 GDE V+ D S V+ + ++ GKY+ TF H D Sbjct: 48 GDEIECIVVSMDDKSKKARVSLRQEDILNALGKYEETFRHVD 89 >SPAC22H10.09 |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 646 Score = 25.0 bits (52), Expect = 9.5 Identities = 8/14 (57%), Positives = 11/14 (78%) Frame = -3 Query: 201 HVLPVHNLHILNCW 160 HVLP+ L +LNC+ Sbjct: 405 HVLPIRQLFLLNCY 418 >SPCC1322.12c |bub1||serine/threonine protein kinase Bub1|Schizosaccharomyces pombe|chr 3|||Manual Length = 1044 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = -3 Query: 243 RHAQTSICTCRRRGHVLPVHNLHILN 166 R A T + R +LPVH LH+ + Sbjct: 776 RQAMTRLKGLRETNSILPVHQLHMFH 801 >SPBC23E6.07c |rfc1||DNA replication factor C complex subunit Rfc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 934 Score = 25.0 bits (52), Expect = 9.5 Identities = 8/20 (40%), Positives = 15/20 (75%) Frame = +2 Query: 464 DVIAKIDDLTQKLTVPTQIW 523 + +A++DD +K + P+QIW Sbjct: 342 ETVARMDDSNKKESQPSQIW 361 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,800,663 Number of Sequences: 5004 Number of extensions: 60516 Number of successful extensions: 172 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 167 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 172 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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