BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060205.seq (653 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain... 29 3.6 At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain... 29 3.6 At4g37060.1 68417.m05248 patatin, putative similar to patatin-li... 29 3.6 At4g25040.1 68417.m03592 integral membrane family protein contai... 28 6.2 At5g37170.1 68418.m04462 O-methyltransferase family 2 protein si... 27 8.2 At5g02150.1 68418.m00136 expressed protein 27 8.2 At2g26340.1 68415.m03160 expressed protein 27 8.2 At2g02790.1 68415.m00222 calmodulin-binding family protein very ... 27 8.2 >At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 870 Score = 28.7 bits (61), Expect = 3.6 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +3 Query: 60 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing protein / RhoGAP domain-containing protein weak similarity to rho-GTPase activating protein [Homo sapiens] GI:14245732; contains Pfam profiles PF00169: PH domain, PF00620: RhoGAP domain Length = 822 Score = 28.7 bits (61), Expect = 3.6 Identities = 17/47 (36%), Positives = 22/47 (46%) Frame = +3 Query: 60 QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200 Q G F DT +Y+ RF+ +D S+L YVN ER R Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735 >At4g37060.1 68417.m05248 patatin, putative similar to patatin-like latex allergen [Hevea brasiliensis][PMID:10589016]; contains patatin domain PF01734 Length = 414 Score = 28.7 bits (61), Expect = 3.6 Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 2/91 (2%) Frame = +2 Query: 275 AKQGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLLEEVIPQV-LCTGKYAPAV 448 A Q + YL L + + L KS L I+L E +L + Q+ + TG Y PA Sbjct: 313 ALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKMLTNRVMQMNIDTGTYEPAA 372 Query: 449 EMDTNDVIAKIDDLTQKLTVPTQIWRKPTDR 541 E ND ++ + L+ ++ RK +D+ Sbjct: 373 ENINND--EQLKRFAKILSEERKLRRKRSDK 401 >At4g25040.1 68417.m03592 integral membrane family protein contains TIGRFAM TIGR01569 : plant integral membrane protein TIGR01569; contains Pfam PF04535 : Domain of unknown function (DUF588) Length = 170 Score = 27.9 bits (59), Expect = 6.2 Identities = 16/47 (34%), Positives = 22/47 (46%) Frame = -3 Query: 594 SRFSVASSHNHFVGKQNERSVGFRQICVGTVNFCVRSSILAITSFVS 454 S FS A + +VGK + G+ IC +C R +I SF S Sbjct: 107 SAFSAAFAEG-YVGKYGNKQAGWLPICGYVHGYCSRVTISLAMSFAS 152 >At5g37170.1 68418.m04462 O-methyltransferase family 2 protein similar to caffeic acid 3-O-methyltransferase [Populus tremuloides][SP|Q00763] Length = 334 Score = 27.5 bits (58), Expect = 8.2 Identities = 10/25 (40%), Positives = 15/25 (60%) Frame = +2 Query: 488 LTQKLTVPTQIWRKPTDRSFCLPTK 562 +T L P +W P++ +F LPTK Sbjct: 38 ITNSLAFPMGVWLSPSEIAFGLPTK 62 >At5g02150.1 68418.m00136 expressed protein Length = 324 Score = 27.5 bits (58), Expect = 8.2 Identities = 10/29 (34%), Positives = 19/29 (65%) Frame = +2 Query: 434 YAPAVEMDTNDVIAKIDDLTQKLTVPTQI 520 +A A++ T DV+ ++ ++TQ L P Q+ Sbjct: 36 FAEAMQSQTVDVVKRLKEITQVLQTPQQV 64 >At2g26340.1 68415.m03160 expressed protein Length = 230 Score = 27.5 bits (58), Expect = 8.2 Identities = 19/54 (35%), Positives = 27/54 (50%) Frame = +2 Query: 329 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTNDVIAKIDDL 490 S ++MKS+L A+ + LL+ V QVL GK V ++ A DDL Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218 >At2g02790.1 68415.m00222 calmodulin-binding family protein very low similarity to SP|P12036 Neurofilament triplet H protein {Homo sapiens}; contains Pfam profile PF00612: IQ calmodulin-binding motif Length = 636 Score = 27.5 bits (58), Expect = 8.2 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%) Frame = +1 Query: 187 NGQYVSTSTASTNRRLSMPTKSRPCSR*RG--KAGR--PAVFAPTHSAHYQI 330 NG S +T ++RR S+P K + G ++GR P+ APT SA +I Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,019,475 Number of Sequences: 28952 Number of extensions: 334517 Number of successful extensions: 841 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 823 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 841 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1363910256 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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