SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060205.seq
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-contain...    29   3.6  
At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-contain...    29   3.6  
At4g37060.1 68417.m05248 patatin, putative similar to patatin-li...    29   3.6  
At4g25040.1 68417.m03592 integral membrane family protein contai...    28   6.2  
At5g37170.1 68418.m04462 O-methyltransferase family 2 protein si...    27   8.2  
At5g02150.1 68418.m00136 expressed protein                             27   8.2  
At2g26340.1 68415.m03160 expressed protein                             27   8.2  
At2g02790.1 68415.m00222 calmodulin-binding family protein very ...    27   8.2  

>At5g19390.2 68418.m02311 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 870

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 17/47 (36%), Positives = 22/47 (46%)
 Frame = +3

Query: 60  QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200
           Q   G F    DT   +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At5g19390.1 68418.m02310 pleckstrin homology (PH) domain-containing
           protein / RhoGAP domain-containing protein weak
           similarity to rho-GTPase activating protein [Homo
           sapiens] GI:14245732; contains Pfam profiles PF00169: PH
           domain, PF00620: RhoGAP domain
          Length = 822

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 17/47 (36%), Positives = 22/47 (46%)
 Frame = +3

Query: 60  QVKIGEFKFGEDTFTLRYVLGDEQPVRFVAKDIASSLKYVNCERAIR 200
           Q   G F    DT   +Y+       RF+ +D  S+L YVN ER  R
Sbjct: 691 QTHFGSFSDARDTH--QYLQNHNPQKRFLQQDFDSTLAYVNHERKQR 735


>At4g37060.1 68417.m05248 patatin, putative similar to patatin-like
           latex allergen [Hevea brasiliensis][PMID:10589016];
           contains patatin domain PF01734
          Length = 414

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 26/91 (28%), Positives = 42/91 (46%), Gaps = 2/91 (2%)
 Frame = +2

Query: 275 AKQGDPLYLH-PHTVLITKSGVIQLIMKSKLPYAIELQEWLLEEVIPQV-LCTGKYAPAV 448
           A Q +  YL      L   +  + L  KS L   I+L E +L   + Q+ + TG Y PA 
Sbjct: 313 ALQSEDKYLRIDDDTLEGDASTLDLSTKSNLENLIKLGEKMLTNRVMQMNIDTGTYEPAA 372

Query: 449 EMDTNDVIAKIDDLTQKLTVPTQIWRKPTDR 541
           E   ND   ++    + L+   ++ RK +D+
Sbjct: 373 ENINND--EQLKRFAKILSEERKLRRKRSDK 401


>At4g25040.1 68417.m03592 integral membrane family protein contains
           TIGRFAM TIGR01569 : plant integral membrane protein
           TIGR01569; contains Pfam PF04535 : Domain of unknown
           function (DUF588)
          Length = 170

 Score = 27.9 bits (59), Expect = 6.2
 Identities = 16/47 (34%), Positives = 22/47 (46%)
 Frame = -3

Query: 594 SRFSVASSHNHFVGKQNERSVGFRQICVGTVNFCVRSSILAITSFVS 454
           S FS A +   +VGK   +  G+  IC     +C R +I    SF S
Sbjct: 107 SAFSAAFAEG-YVGKYGNKQAGWLPICGYVHGYCSRVTISLAMSFAS 152


>At5g37170.1 68418.m04462 O-methyltransferase family 2 protein
           similar to caffeic acid 3-O-methyltransferase [Populus
           tremuloides][SP|Q00763]
          Length = 334

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 10/25 (40%), Positives = 15/25 (60%)
 Frame = +2

Query: 488 LTQKLTVPTQIWRKPTDRSFCLPTK 562
           +T  L  P  +W  P++ +F LPTK
Sbjct: 38  ITNSLAFPMGVWLSPSEIAFGLPTK 62


>At5g02150.1 68418.m00136 expressed protein
          Length = 324

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 10/29 (34%), Positives = 19/29 (65%)
 Frame = +2

Query: 434 YAPAVEMDTNDVIAKIDDLTQKLTVPTQI 520
           +A A++  T DV+ ++ ++TQ L  P Q+
Sbjct: 36  FAEAMQSQTVDVVKRLKEITQVLQTPQQV 64


>At2g26340.1 68415.m03160 expressed protein
          Length = 230

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 19/54 (35%), Positives = 27/54 (50%)
 Frame = +2

Query: 329 SGVIQLIMKSKLPYAIELQEWLLEEVIPQVLCTGKYAPAVEMDTNDVIAKIDDL 490
           S    ++MKS+L  A+   + LL+ V  QVL  GK    V    ++  A  DDL
Sbjct: 165 SNATVVLMKSQLGTALTALDSLLQTVPSQVLDKGKAMVEVYRSASEEDAGSDDL 218


>At2g02790.1 68415.m00222 calmodulin-binding family protein very low
           similarity to SP|P12036 Neurofilament triplet H protein
           {Homo sapiens}; contains Pfam profile PF00612: IQ
           calmodulin-binding motif
          Length = 636

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
 Frame = +1

Query: 187 NGQYVSTSTASTNRRLSMPTKSRPCSR*RG--KAGR--PAVFAPTHSAHYQI 330
           NG   S +T  ++RR S+P K     +  G  ++GR  P+  APT SA  +I
Sbjct: 472 NGNVASENTKPSDRRASLPAKIENHHQDDGLTQSGRKIPSYMAPTASAKARI 523


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,019,475
Number of Sequences: 28952
Number of extensions: 334517
Number of successful extensions: 841
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 841
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -