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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060202.seq
         (656 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g05750.1 68416.m00646 expressed protein                             29   2.7  
At5g58280.1 68418.m07296 transcriptional factor B3 family protei...    29   3.6  
At2g43930.1 68415.m05460 protein kinase family protein contains ...    29   3.6  
At5g24550.1 68418.m02899 glycosyl hydrolase family 1 protein con...    28   6.3  
At3g19470.1 68416.m02468 F-box family protein contains F-box dom...    28   6.3  
At5g24540.1 68418.m02898 glycosyl hydrolase family 1 protein con...    27   8.3  
At3g23780.1 68416.m02989 DNA-directed RNA polymerase family prot...    27   8.3  
At3g23420.1 68416.m02952 F-box family protein-related contains T...    27   8.3  
At3g22770.1 68416.m02871 F-box family protein-related contains T...    27   8.3  

>At3g05750.1 68416.m00646 expressed protein
          Length = 798

 Score = 29.1 bits (62), Expect = 2.7
 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%)
 Frame = +2

Query: 296 EYVNNFQQFLNT-IRNNFNGPCAKHDMGSSCEDTEEATEKQAVQQTLDGHDWVLE 457
           E VN+F    N  I  + +   +++D   + E+TE  +E  A+ +  +GHDW LE
Sbjct: 592 EEVNSFSTAENLQISCSTSFSSSRNDYHHNIEETE-LSESVALSEAEEGHDWELE 645


>At5g58280.1 68418.m07296 transcriptional factor B3 family protein
           contains Pfam profile PF02362: B3 DNA binding domain
          Length = 273

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 5/74 (6%)
 Frame = +1

Query: 412 TGSSTNIRRPR----LGVGK*RFLHFCKTFHFEEAL*VIQKYINFENFFKSTDPGYINKC 579
           TG ++N+RR R          R LH CK   +EE +   +    F+   KS  P ++   
Sbjct: 72  TGRTSNLRRSRHSSTWATYISRPLHECKFASYEEKVGAFKAAEKFQRSLKSPHPYFVKSM 131

Query: 580 VQAGDY-YYWPNWP 618
           V++  Y  +W   P
Sbjct: 132 VRSHVYSCFWLGLP 145


>At2g43930.1 68415.m05460 protein kinase family protein contains
           similarity to NPK1-related protein kinase 2 GI:2342425
           from [Arabidopsis thaliana]
          Length = 204

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 13/28 (46%), Positives = 18/28 (64%)
 Frame = -3

Query: 111 NHHPFLHRIKTLIKDFNNTLLFGAYIQI 28
           +HH  LH IK LI++ + TLLF   + I
Sbjct: 2   DHHRRLHTIKILIRNSSTTLLFFKIVPI 29


>At5g24550.1 68418.m02899 glycosyl hydrolase family 1 protein
           contains Pfam PF00232 : Glycosyl hydrolase family 1
           domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
           similar to anther-specific protein ATA27 (GI:2746341)
           [Arabidopsis thaliana]
          Length = 534

 Score = 27.9 bits (59), Expect = 6.3
 Identities = 11/32 (34%), Positives = 18/32 (56%)
 Frame = +2

Query: 260 IRNTRTGTRRLFEYVNNFQQFLNTIRNNFNGP 355
           I +   GT+ L+ Y    ++ LN I+N +N P
Sbjct: 380 ISSESDGTKILWSYPEGLRKLLNYIKNKYNNP 411


>At3g19470.1 68416.m02468 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 382

 Score = 27.9 bits (59), Expect = 6.3
 Identities = 15/37 (40%), Positives = 21/37 (56%)
 Frame = -2

Query: 178 VVNHFGYRVYIFRVNLFNNFDQKSPSIFT*NKNANKR 68
           VV    +RVY+ RVNL NN  + S S  + + +  KR
Sbjct: 59  VVLMMNFRVYLMRVNLQNNVVESSSSSSSSSSSCIKR 95


>At5g24540.1 68418.m02898 glycosyl hydrolase family 1 protein
           contains Pfam PF00232 : Glycosyl hydrolase family 1
           domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
           similar to amygdalin hydrolase isoform AH I precursor
           (GI:16757966) [Prunus serotina]
          Length = 534

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 10/26 (38%), Positives = 16/26 (61%)
 Frame = +2

Query: 278 GTRRLFEYVNNFQQFLNTIRNNFNGP 355
           GT+ L+ Y    ++ LN I+N +N P
Sbjct: 386 GTKILWSYPEGLRKILNYIKNKYNNP 411


>At3g23780.1 68416.m02989 DNA-directed RNA polymerase family protein
           similar to SP|P38420 DNA-directed RNA polymerase II 135
           kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit
           2) {Arabidopsis thaliana}; contains Pfam profiles
           PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA
           polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2
           domain 3, PF04566: RNA polymerase Rpb2 domain 4,
           PF04567: RNA polymerase Rpb2 domain 5
          Length = 946

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 3/44 (6%)
 Frame = -1

Query: 584 CTHLLMYPGSVLLKKFSKLIYF---CMTYSASSK*KVLQKCKNR 462
           C HL ++PG   LKK ++ + +   C+  S + K    +KC+NR
Sbjct: 131 CLHLYLFPGLQSLKKKARFLGYMVKCLLNSYAGK----RKCENR 170


>At3g23420.1 68416.m02952 F-box family protein-related contains
           TIGRFAM TIGR01640: F-box protein interaction domain
          Length = 285

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 13/35 (37%), Positives = 20/35 (57%)
 Frame = -2

Query: 220 VLSSGTSFKKKL*NVVNHFGYRVYIFRVNLFNNFD 116
           V  +  + KK+   VV    +RVY+ R+NL NN +
Sbjct: 39  VCQAKVAAKKREYKVVMMMDFRVYLMRINLQNNVE 73


>At3g22770.1 68416.m02871 F-box family protein-related contains
           TIGRFAM TIGR01640: F-box protein interaction domain
          Length = 327

 Score = 27.5 bits (58), Expect = 8.3
 Identities = 13/26 (50%), Positives = 16/26 (61%)
 Frame = -2

Query: 178 VVNHFGYRVYIFRVNLFNNFDQKSPS 101
           VV    +RVY+ RVNL NN  + S S
Sbjct: 2   VVMMMNFRVYLMRVNLDNNVVESSSS 27


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,622,495
Number of Sequences: 28952
Number of extensions: 270520
Number of successful extensions: 643
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 643
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1373722560
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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