BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060202.seq (656 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g05750.1 68416.m00646 expressed protein 29 2.7 At5g58280.1 68418.m07296 transcriptional factor B3 family protei... 29 3.6 At2g43930.1 68415.m05460 protein kinase family protein contains ... 29 3.6 At5g24550.1 68418.m02899 glycosyl hydrolase family 1 protein con... 28 6.3 At3g19470.1 68416.m02468 F-box family protein contains F-box dom... 28 6.3 At5g24540.1 68418.m02898 glycosyl hydrolase family 1 protein con... 27 8.3 At3g23780.1 68416.m02989 DNA-directed RNA polymerase family prot... 27 8.3 At3g23420.1 68416.m02952 F-box family protein-related contains T... 27 8.3 At3g22770.1 68416.m02871 F-box family protein-related contains T... 27 8.3 >At3g05750.1 68416.m00646 expressed protein Length = 798 Score = 29.1 bits (62), Expect = 2.7 Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 1/55 (1%) Frame = +2 Query: 296 EYVNNFQQFLNT-IRNNFNGPCAKHDMGSSCEDTEEATEKQAVQQTLDGHDWVLE 457 E VN+F N I + + +++D + E+TE +E A+ + +GHDW LE Sbjct: 592 EEVNSFSTAENLQISCSTSFSSSRNDYHHNIEETE-LSESVALSEAEEGHDWELE 645 >At5g58280.1 68418.m07296 transcriptional factor B3 family protein contains Pfam profile PF02362: B3 DNA binding domain Length = 273 Score = 28.7 bits (61), Expect = 3.6 Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 5/74 (6%) Frame = +1 Query: 412 TGSSTNIRRPR----LGVGK*RFLHFCKTFHFEEAL*VIQKYINFENFFKSTDPGYINKC 579 TG ++N+RR R R LH CK +EE + + F+ KS P ++ Sbjct: 72 TGRTSNLRRSRHSSTWATYISRPLHECKFASYEEKVGAFKAAEKFQRSLKSPHPYFVKSM 131 Query: 580 VQAGDY-YYWPNWP 618 V++ Y +W P Sbjct: 132 VRSHVYSCFWLGLP 145 >At2g43930.1 68415.m05460 protein kinase family protein contains similarity to NPK1-related protein kinase 2 GI:2342425 from [Arabidopsis thaliana] Length = 204 Score = 28.7 bits (61), Expect = 3.6 Identities = 13/28 (46%), Positives = 18/28 (64%) Frame = -3 Query: 111 NHHPFLHRIKTLIKDFNNTLLFGAYIQI 28 +HH LH IK LI++ + TLLF + I Sbjct: 2 DHHRRLHTIKILIRNSSTTLLFFKIVPI 29 >At5g24550.1 68418.m02899 glycosyl hydrolase family 1 protein contains Pfam PF00232 : Glycosyl hydrolase family 1 domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase; similar to anther-specific protein ATA27 (GI:2746341) [Arabidopsis thaliana] Length = 534 Score = 27.9 bits (59), Expect = 6.3 Identities = 11/32 (34%), Positives = 18/32 (56%) Frame = +2 Query: 260 IRNTRTGTRRLFEYVNNFQQFLNTIRNNFNGP 355 I + GT+ L+ Y ++ LN I+N +N P Sbjct: 380 ISSESDGTKILWSYPEGLRKLLNYIKNKYNNP 411 >At3g19470.1 68416.m02468 F-box family protein contains F-box domain Pfam:PF00646 Length = 382 Score = 27.9 bits (59), Expect = 6.3 Identities = 15/37 (40%), Positives = 21/37 (56%) Frame = -2 Query: 178 VVNHFGYRVYIFRVNLFNNFDQKSPSIFT*NKNANKR 68 VV +RVY+ RVNL NN + S S + + + KR Sbjct: 59 VVLMMNFRVYLMRVNLQNNVVESSSSSSSSSSSCIKR 95 >At5g24540.1 68418.m02898 glycosyl hydrolase family 1 protein contains Pfam PF00232 : Glycosyl hydrolase family 1 domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase; similar to amygdalin hydrolase isoform AH I precursor (GI:16757966) [Prunus serotina] Length = 534 Score = 27.5 bits (58), Expect = 8.3 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = +2 Query: 278 GTRRLFEYVNNFQQFLNTIRNNFNGP 355 GT+ L+ Y ++ LN I+N +N P Sbjct: 386 GTKILWSYPEGLRKILNYIKNKYNNP 411 >At3g23780.1 68416.m02989 DNA-directed RNA polymerase family protein similar to SP|P38420 DNA-directed RNA polymerase II 135 kDa polypeptide (EC 2.7.7.6) (RNA polymerase II subunit 2) {Arabidopsis thaliana}; contains Pfam profiles PF04560: RNA polymerase Rpb2 domain 7, PF04561: RNA polymerase Rpb2 domain 2, PF04565: RNA polymerase Rpb2 domain 3, PF04566: RNA polymerase Rpb2 domain 4, PF04567: RNA polymerase Rpb2 domain 5 Length = 946 Score = 27.5 bits (58), Expect = 8.3 Identities = 15/44 (34%), Positives = 25/44 (56%), Gaps = 3/44 (6%) Frame = -1 Query: 584 CTHLLMYPGSVLLKKFSKLIYF---CMTYSASSK*KVLQKCKNR 462 C HL ++PG LKK ++ + + C+ S + K +KC+NR Sbjct: 131 CLHLYLFPGLQSLKKKARFLGYMVKCLLNSYAGK----RKCENR 170 >At3g23420.1 68416.m02952 F-box family protein-related contains TIGRFAM TIGR01640: F-box protein interaction domain Length = 285 Score = 27.5 bits (58), Expect = 8.3 Identities = 13/35 (37%), Positives = 20/35 (57%) Frame = -2 Query: 220 VLSSGTSFKKKL*NVVNHFGYRVYIFRVNLFNNFD 116 V + + KK+ VV +RVY+ R+NL NN + Sbjct: 39 VCQAKVAAKKREYKVVMMMDFRVYLMRINLQNNVE 73 >At3g22770.1 68416.m02871 F-box family protein-related contains TIGRFAM TIGR01640: F-box protein interaction domain Length = 327 Score = 27.5 bits (58), Expect = 8.3 Identities = 13/26 (50%), Positives = 16/26 (61%) Frame = -2 Query: 178 VVNHFGYRVYIFRVNLFNNFDQKSPS 101 VV +RVY+ RVNL NN + S S Sbjct: 2 VVMMMNFRVYLMRVNLDNNVVESSSS 27 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,622,495 Number of Sequences: 28952 Number of extensions: 270520 Number of successful extensions: 643 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 630 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 643 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1373722560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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