BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060197.seq (511 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 32 0.043 SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit... 32 0.043 SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces po... 31 0.076 SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces ... 27 2.2 SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces... 26 2.9 SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex su... 25 6.6 SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog... 25 8.7 >SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases |Schizosaccharomyces pombe|chr 1|||Manual Length = 450 Score = 32.3 bits (70), Expect = 0.043 Identities = 19/63 (30%), Positives = 32/63 (50%) Frame = +2 Query: 227 SHTHLPCYRGITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGS 406 S T + GI++AD+ V++ + H++ L L NV RWF + HQ V S Sbjct: 134 SSTFIAQDSGISVADLAVYARI-HSYICGLSAKEGYKLNNVCRWFDFIQHQESVMEAANS 192 Query: 407 LTL 415 +++ Sbjct: 193 MSM 195 >SPAC29A4.02c |||translation elongation factor EF-1 gamma subunit |Schizosaccharomyces pombe|chr 1|||Manual Length = 409 Score = 32.3 bits (70), Expect = 0.043 Identities = 22/83 (26%), Positives = 39/83 (46%) Frame = +2 Query: 224 SSHTHLPCYRGITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVG 403 +S T+L R +TLAD+ L +VL S + ++ R++ T+ HQ ++ A+ Sbjct: 145 ASKTYLVGSR-LTLADIFFTCFLKFGATYVLTKSYLAKYTHIYRYYQTIYHQAKLDAITE 203 Query: 404 SLTLCAAPPTYXPKKYQELAGAQ 472 L P + +E A A+ Sbjct: 204 PLKFIDQPLPIIKAENKEAAPAK 226 >SPCC1183.02 |||glutathione S-transferase |Schizosaccharomyces pombe|chr 3|||Manual Length = 220 Score = 31.5 bits (68), Expect = 0.076 Identities = 14/43 (32%), Positives = 25/43 (58%) Frame = +2 Query: 260 TLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQV 388 TLAD+ S L F ++D R L ++ R+++T+ HQ ++ Sbjct: 161 TLADLFFGSLLRIFFNSIIDEKTRKELPHLTRYYITMFHQAKL 203 >SPAC14C4.04 |B22918-2||hypothetical protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 267 Score = 26.6 bits (56), Expect = 2.2 Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%) Frame = +1 Query: 52 IWLPKPVSGSGXHGLTANYCL-LPALGSSLTLV 147 +W+P PV SG T C+ LP G +T V Sbjct: 23 LWVPTPVGRSGRKHRTLKMCIVLPDTGYDVTQV 55 >SPAC16.05c |sfp1||transcription factor Sfp1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 442 Score = 26.2 bits (55), Expect = 2.9 Identities = 11/19 (57%), Positives = 13/19 (68%) Frame = +1 Query: 190 TYWPP*KYWTDIFSHAPSL 246 TY PP Y D+FS APS+ Sbjct: 257 TYLPPFNYDHDVFSFAPSV 275 >SPAC6F12.16c |mtr4||ATP-dependent RNA helicase, TRAMP complex subunit Mtr4|Schizosaccharomyces pombe|chr 1|||Manual Length = 1117 Score = 25.0 bits (52), Expect = 6.6 Identities = 15/54 (27%), Positives = 27/54 (50%) Frame = +3 Query: 99 SELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFLVTEES 260 ++L P CA + L + ++ +R K +L LK+L + R V++ES Sbjct: 972 NDLTPEQCAALLSCLVFQEKSEVENQRMKEELAGPLKILQ-EMARRIAKVSKES 1024 >SPBC19G7.01c |msh2|swi8, mut3, SPBC24C6.12c|MutS protein homolog 2|Schizosaccharomyces pombe|chr 2|||Manual Length = 982 Score = 24.6 bits (51), Expect = 8.7 Identities = 10/23 (43%), Positives = 14/23 (60%) Frame = +1 Query: 106 YCLLPALGSSLTLVSCNSTNRML 174 + L+P LG L + SCN+ R L Sbjct: 261 HALVPELGLQLAMASCNALLRYL 283 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,024,726 Number of Sequences: 5004 Number of extensions: 39584 Number of successful extensions: 125 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 116 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 125 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 204242806 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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