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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060197.seq
         (511 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ...    43   1e-04
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ...    40   0.001
At1g16840.2 68414.m02028 expressed protein                             31   0.60 
At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N domain-...    29   1.8  
At3g49210.1 68416.m05378 expressed protein                             29   2.4  
At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1 ...    28   4.2  
At1g47220.1 68414.m05227 cyclin, putative similar to cyclin A-li...    28   4.2  
At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik...    28   4.2  
At1g63230.1 68414.m07147 pentatricopeptide (PPR) repeat-containi...    27   7.3  

>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
           eEF-1B gamma, putative Similar to elongation factor
           1-gamma (gb|EF1G_XENLA). ESTs
           gb|T20564,gb|T45940,gb|T04527 come from this gene
          Length = 414

 Score = 42.7 bits (96), Expect = 1e-04
 Identities = 26/75 (34%), Positives = 39/75 (52%)
 Frame = +2

Query: 224 SSHTHLPCYRGITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVG 403
           +S+T+L  +  ITLAD+I    L   F  V+     S   +V+R+F TV +QP  + V+G
Sbjct: 146 TSNTYLVGH-SITLADIITVCNLNLGFATVMTKKFTSEFPHVERYFWTVVNQPNFTKVLG 204

Query: 404 SLTLCAAPPTYXPKK 448
            +    A P    KK
Sbjct: 205 DVKQTEAVPPIASKK 219


>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
           eEF-1B gamma, putative similar to elongation factor 1B
           gamma GI:3868758 from [Oryza sativa]
          Length = 413

 Score = 39.9 bits (89), Expect = 0.001
 Identities = 20/64 (31%), Positives = 32/64 (50%)
 Frame = +2

Query: 257 ITLADVIVFSTLLHAFQHVLDPSVRSSLINVQRWFLTVAHQPQVSAVVGSLTLCAAPPTY 436
           +TLAD++    L   F  V+     S+  +V+R+F T+ +QP+   V+G      A P  
Sbjct: 156 VTLADIVTICNLNLGFATVMTKKFTSAFPHVERYFWTMVNQPEFKKVLGDAKQTEAVPPV 215

Query: 437 XPKK 448
             KK
Sbjct: 216 PTKK 219


>At1g16840.2 68414.m02028 expressed protein
          Length = 161

 Score = 30.7 bits (66), Expect = 0.60
 Identities = 20/61 (32%), Positives = 26/61 (42%)
 Frame = +1

Query: 37  VSAEEIWLPKPVSGSGXHGLTANYCLLPALGSSLTLVSCNSTNRMLNVQSLTYWPP*KYW 216
           V     W+P P SGS  HG+     L+  L + LT     STN      S  Y+    YW
Sbjct: 83  VPGASYWVPPPGSGSQSHGIAQ---LVVKLANPLTHEESLSTNSSHGWPSSDYFLKGSYW 139

Query: 217 T 219
           +
Sbjct: 140 S 140


>At2g33000.1 68415.m04044 ubiquitin-associated (UBA)/TS-N
           domain-containing protein-related contains weak hit to
           Pfam F00627: UBA/TS-N domain; supported by tandem
           duplication of ubiquitin-associated (UBA)/TS-N domain
           protein (GI:2924790) (TIGR_Ath1:At2g33010) [Arabidopsis
           thaliana]
          Length = 508

 Score = 29.1 bits (62), Expect = 1.8
 Identities = 11/31 (35%), Positives = 16/31 (51%)
 Frame = +1

Query: 142 LVSCNSTNRMLNVQSLTYWPP*KYWTDIFSH 234
           L++ +  N   N +S + WPP K W D   H
Sbjct: 195 LMASSQVNTQENQESTSVWPPMKTWVDRICH 225


>At3g49210.1 68416.m05378 expressed protein
          Length = 518

 Score = 28.7 bits (61), Expect = 2.4
 Identities = 16/64 (25%), Positives = 30/64 (46%)
 Frame = +3

Query: 51  DLATQARVWQWXSWSDSELLPASCAWVFPYLGIMQFNKQNVERAKSDLLAALKVLDGHLL 230
           D+  +    +W +W    + P S       L  ++  K+ ++R K+ L AAL  + G  +
Sbjct: 352 DMMAKGSTCRWGNWIGYIVFPFSIGLRDDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFI 411

Query: 231 TRTF 242
            +TF
Sbjct: 412 LKTF 415


>At3g01510.1 68416.m00077 5'-AMP-activated protein kinase beta-1
           subunit-related contains similarity to Swiss-Prot:P80387
           5'-AMP-activated protein kinase, beta-1 subunit (AMPK
           beta-1 chain) (AMPKb) (40 kDa subunit) [Sus scrofa]
          Length = 591

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 1/70 (1%)
 Frame = -1

Query: 418 TERERADDGRHLRLVGDGQEPTLNVYQRRTDARV*HVLESMQQCTEDNDIGKCDSSVTRK 239
           TER+   +  ++ +VGD       + Q R DA +  V+E +   +E   + K    +   
Sbjct: 522 TERDDRGNTNNIIVVGDVANVRPTIQQPRKDANIIKVIERVLTESERFRLAKAARCIAFS 581

Query: 238 V-RVRRCPSS 212
           V  +R CP S
Sbjct: 582 VCPIRLCPKS 591


>At1g47220.1 68414.m05227 cyclin, putative similar to cyclin A-like
           protein [Nicotiana tabacum] GI:1064931, A-type cyclin
           [Catharanthus roseus] GI:2190259; contains Pfam profiles
           PF00134: Cyclin, N-terminal domain, PF02984: Cyclin,
           C-terminal domain
          Length = 327

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 6/64 (9%)
 Frame = +3

Query: 132 FPYLGIMQFNKQNVERAKSDLLAALKVLDGHLLTRTFL------VTEESHLPMSLSSVHC 293
           F Y+    + KQ+V + + D+L AL+   G   T TFL        E+  +P       C
Sbjct: 159 FCYITANTYTKQDVLKMEEDILLALEFELGRPTTNTFLRRFIRVAQEDFKVPNLQLEPLC 218

Query: 294 CMLS 305
           C LS
Sbjct: 219 CYLS 222


>At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like
           transposon protein GB:AAB95292 GI:2088658 from
           [Arabidopsis thaliana]
          Length = 1148

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 14/28 (50%), Positives = 18/28 (64%)
 Frame = -3

Query: 407 ASRRRPTLAAGGRRSGTNAERLSATNGR 324
           +S R P+  A GRRSGT   R+S T G+
Sbjct: 171 SSARHPS-PASGRRSGTPVRRISPTPGK 197


>At1g63230.1 68414.m07147 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 323

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 13/33 (39%), Positives = 20/33 (60%)
 Frame = +2

Query: 251 RGITLADVIVFSTLLHAFQHVLDPSVRSSLINV 349
           RGI +A+ + ++TL+H F  V D      L+NV
Sbjct: 245 RGI-VANTVTYTTLIHGFCQVGDLDAAQDLLNV 276


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,155,383
Number of Sequences: 28952
Number of extensions: 229069
Number of successful extensions: 613
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 602
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 613
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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