BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060194.seq (653 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|... 28 1.4 SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomy... 25 7.2 SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|ch... 25 7.2 SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyce... 25 7.2 SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual 25 7.2 SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein P... 25 9.5 SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1||... 25 9.5 >SPAC27E2.04c |mug155||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 187 Score = 27.9 bits (59), Expect = 1.4 Identities = 10/16 (62%), Positives = 12/16 (75%) Frame = +2 Query: 344 LNEAYCRTKLKCRSKK 391 L E YCR K++CR KK Sbjct: 58 LKEIYCRLKIRCRLKK 73 >SPAC1687.16c |erg3||C-5 sterol desaturase Erg3 |Schizosaccharomyces pombe|chr 1|||Manual Length = 300 Score = 25.4 bits (53), Expect = 7.2 Identities = 12/40 (30%), Positives = 18/40 (45%) Frame = -1 Query: 590 LYSSFNISGG*LYFSNRYSLPRL*SRCSLWVYSVPRYSHS 471 ++S F I + +RY PRL W+ P SH+ Sbjct: 127 MFSDFGIYWAHRFLHHRYVYPRLHKLHHKWIICTPYASHA 166 >SPCC736.06 |||aspartate-tRNA ligase|Schizosaccharomyces pombe|chr 3|||Manual Length = 611 Score = 25.4 bits (53), Expect = 7.2 Identities = 12/16 (75%), Positives = 13/16 (81%) Frame = +1 Query: 580 LEYKPNFAVRGLHYDI 627 LE KP +VRGLHYDI Sbjct: 482 LEKKP-LSVRGLHYDI 496 >SPBP8B7.18c |||phosphomethylpyrimidine kinase|Schizosaccharomyces pombe|chr 2|||Manual Length = 551 Score = 25.4 bits (53), Expect = 7.2 Identities = 11/40 (27%), Positives = 20/40 (50%) Frame = +2 Query: 422 FACNELDLSEVQVYGFDYDYTLAHYKPTMNTCSIILVENI 541 F E L+E +G DY + + P +N CS ++ ++ Sbjct: 271 FLAWEHSLTEAVQFGIDYVHGAITHSPPINNCSTNILNHM 310 >SPBC839.08c |its8||pig-N |Schizosaccharomyces pombe|chr 2|||Manual Length = 935 Score = 25.4 bits (53), Expect = 7.2 Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 1/64 (1%) Frame = +3 Query: 96 WKF*IRLIFLFVHKCCK-NWKLSAHLL*FYNNSKLSVKMSLLISSVGNLFLGVKPLARV* 272 W + + + F HK +W + LL F+ ++ K SLL G + V + + Sbjct: 587 WPWLLHADYAFSHKTISVSWSVLTSLLCFFTILPVNKKESLLFIFAGGFAMSVAGVFYIL 646 Query: 273 FRKH 284 +R++ Sbjct: 647 YRRN 650 >SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein Pvg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 389 Score = 25.0 bits (52), Expect = 9.5 Identities = 14/41 (34%), Positives = 20/41 (48%) Frame = +2 Query: 161 SAFIIILQ*FKTFGKNVFVNQFGW*FISWSQTSRTGLIQKT 283 S FI + Q F F + V+ W F+SWS L+ K+ Sbjct: 131 SNFIELSQ-FTAFEVELRVSHPNWAFVSWSHDDLNELVDKS 170 >SPAC1006.06 |rgf2||RhoGEF Rgf2|Schizosaccharomyces pombe|chr 1|||Manual Length = 1158 Score = 25.0 bits (52), Expect = 9.5 Identities = 13/31 (41%), Positives = 18/31 (58%) Frame = -2 Query: 610 LEQQNWVCIPVSIFLEGNCTSLTDILYQDYR 518 LEQQ + + V L G TS+TD +D+R Sbjct: 397 LEQQARLHLKVQPVLSGGSTSITDKQEEDHR 427 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,830,341 Number of Sequences: 5004 Number of extensions: 62077 Number of successful extensions: 168 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 163 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 168 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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