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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060194.seq
         (653 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g23890.1 68415.m02853 5' nucleotidase family protein contains...    50   1e-06
At5g48960.1 68418.m06057 5' nucleotidase family protein low simi...    38   0.008
At1g75210.1 68414.m08737 5' nucleotidase family protein contains...    31   0.67 
At5g24320.2 68418.m02866 WD-40 repeat family protein contains Pf...    29   2.0  
At5g24320.1 68418.m02865 WD-40 repeat family protein contains Pf...    29   2.0  
At2g34710.1 68415.m04263 homeobox-leucine zipper transcription f...    29   3.6  
At4g30080.1 68417.m04278 transcriptional factor B3 family protei...    28   4.7  
At3g56400.1 68416.m06272 WRKY family transcription factor DNA-bi...    27   8.2  
At2g15980.1 68415.m01829 pentatricopeptide (PPR) repeat-containi...    27   8.2  

>At2g23890.1 68415.m02853 5' nucleotidase family protein contains
           Pfam PF05761: 5' nucleotidase family; similar to
           Cytosolic purine 5'-nucleotidase (EC 3.1.3.5)
           (5'-nucleotidase cytosolic II) (Swiss-Prot:P49902) [Homo
           sapiens]
          Length = 553

 Score = 50.4 bits (115), Expect = 1e-06
 Identities = 19/31 (61%), Positives = 24/31 (77%)
 Frame = +2

Query: 404 VNPKGVFACNELDLSEVQVYGFDYDYTLAHY 496
           +NP+G++    L L  +QVYGFDYDYTLAHY
Sbjct: 94  MNPQGIYVNKNLRLDNIQVYGFDYDYTLAHY 124



 Score = 48.4 bits (110), Expect = 4e-06
 Identities = 18/50 (36%), Positives = 32/50 (64%)
 Frame = +1

Query: 499 THNEHLLYNLGREYLLEKYNYPPEILKLEYKPNFAVRGLHYDIEKGTSFK 648
           +H + L+Y+L +++++ ++ YP    + EY P F +RGL+YD  KG   K
Sbjct: 126 SHLQSLIYDLAKKHMVNEFRYPDVCTQFEYDPTFPIRGLYYDKLKGCLMK 175


>At5g48960.1 68418.m06057 5' nucleotidase family protein low
           similarity to SP|P49902 Cytosolic purine 5'-nucleotidase
           (EC 3.1.3.5) (5'-nucleotidase cytosolic II) {Homo
           sapiens}; contains Pfam profile PF05761: 5' nucleotidase
           family
          Length = 642

 Score = 37.5 bits (83), Expect = 0.008
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +2

Query: 407 NPKGVFACNELDLSEVQVYGFDYDYTLAHY 496
           +P+G+F    L+L  +   G+D DYTL HY
Sbjct: 161 SPRGIFCSRTLNLRSISAIGYDMDYTLMHY 190


>At1g75210.1 68414.m08737 5' nucleotidase family protein contains
           Pfam profile: PF05761 5' nucleotidase family
          Length = 642

 Score = 31.1 bits (67), Expect = 0.67
 Identities = 12/27 (44%), Positives = 16/27 (59%)
 Frame = +2

Query: 419 VFACNELDLSEVQVYGFDYDYTLAHYK 499
           +F    L++  +   GFD DYTLA YK
Sbjct: 149 IFCNRSLNMKNIIAVGFDMDYTLAQYK 175



 Score = 29.1 bits (62), Expect = 2.7
 Identities = 16/47 (34%), Positives = 24/47 (51%)
 Frame = +1

Query: 508 EHLLYNLGREYLLEKYNYPPEILKLEYKPNFAVRGLHYDIEKGTSFK 648
           E L Y+     L+    YP E+L+  +  N+ VRGL  D ++G   K
Sbjct: 180 ESLAYDGTVRKLVYDLGYPNELLEWTFDWNYMVRGLVLDKKRGNILK 226


>At5g24320.2 68418.m02866 WD-40 repeat family protein contains Pfam
           PF00400: WD domain, G-beta repeat; similar to WD-repeat
           protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
           sapiens]
          Length = 698

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +1

Query: 517 LYNLGREYLLE----KYNYPPEILKLEYKPNFAVRGLHYDIEKGTSFKT 651
           L++LGREY+L+       +P E L   + P  A+R  HY   + +  +T
Sbjct: 618 LFSLGREYVLDSPKGSATWPEEKLASSFSPVKAIRRSHYKFLRSSCRRT 666


>At5g24320.1 68418.m02865 WD-40 repeat family protein contains Pfam
           PF00400: WD domain, G-beta repeat; similar to WD-repeat
           protein 5 (WD repeat protein BIG-3) (SP: Q9UGP9) [Homo
           sapiens]
          Length = 694

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = +1

Query: 517 LYNLGREYLLE----KYNYPPEILKLEYKPNFAVRGLHYDIEKGTSFKT 651
           L++LGREY+L+       +P E L   + P  A+R  HY   + +  +T
Sbjct: 614 LFSLGREYVLDSPKGSATWPEEKLASSFSPVKAIRRSHYKFLRSSCRRT 662


>At2g34710.1 68415.m04263 homeobox-leucine zipper transcription
           factor (HB-14) identical to homeodomain transcription
           factor (ATHB-14)GP:3132474 GB:Y11122 [Arabidopsis
           thaliana];
          Length = 852

 Score = 28.7 bits (61), Expect = 3.6
 Identities = 28/110 (25%), Positives = 49/110 (44%), Gaps = 3/110 (2%)
 Frame = +2

Query: 275 QKTLKYCEVKSALSLKKYSTRELLNEAYCRTKLKCRSKKLPQDVNPKGVFACNELDLSE- 451
           Q+ ++ C + S +  K+           CR K +  + +L Q VN K + A N+L + E 
Sbjct: 53  QQLIRECPILSNIEPKQIKV--WFQNRRCREKQRKEAARL-QTVNRK-LNAMNKLLMEEN 108

Query: 452 --VQVYGFDYDYTLAHYKPTMNTCSIILVENIC*RSTITLQKY*NWNTNP 595
             +Q    +  Y   H K  ++T S    +N C    ++ Q++   N NP
Sbjct: 109 DRLQKQVSNLVYENGHMKHQLHTASGTTTDNSCESVVVSGQQHQQQNPNP 158


>At4g30080.1 68417.m04278 transcriptional factor B3 family protein
           contains Pfam profile: PF02362 B3 DNA binding domain
          Length = 670

 Score = 28.3 bits (60), Expect = 4.7
 Identities = 17/54 (31%), Positives = 22/54 (40%), Gaps = 4/54 (7%)
 Frame = +1

Query: 502 HNEHLLYNLG----REYLLEKYNYPPEILKLEYKPNFAVRGLHYDIEKGTSFKT 651
           HN H  Y L       Y L +   PP    L+  P+  +R +    EKG  F T
Sbjct: 450 HNAHQYYGLSSSDLHHYYLNRPPPPPPPSSLQLSPSLGLRNIDTKNEKGFCFLT 503


>At3g56400.1 68416.m06272 WRKY family transcription factor
           DNA-binding protein 4 WRKY4 - Nicotiana tabacum,
           EMBL:AF193771
          Length = 294

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 11/36 (30%), Positives = 21/36 (58%)
 Frame = +2

Query: 281 TLKYCEVKSALSLKKYSTRELLNEAYCRTKLKCRSK 388
           T++   ++ A S +KY  +E+LN  + R+  +C  K
Sbjct: 113 TIESTILEDAFSWRKYGQKEILNAKFPRSYFRCTHK 148


>At2g15980.1 68415.m01829 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 498

 Score = 27.5 bits (58), Expect = 8.2
 Identities = 15/61 (24%), Positives = 32/61 (52%)
 Frame = +2

Query: 239 ISWSQTSRTGLIQKTLKYCEVKSALSLKKYSTRELLNEAYCRTKLKCRSKKLPQDVNPKG 418
           +S+ +   T ++++  +  E +   S   YS   L+ EAYC   L   ++K+ +++  +G
Sbjct: 253 VSFYREGETEMVERIWREMEEEVGCSPNVYSYNVLM-EAYCARGLMSEAEKVWEEMKVRG 311

Query: 419 V 421
           V
Sbjct: 312 V 312


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,961,979
Number of Sequences: 28952
Number of extensions: 289450
Number of successful extensions: 694
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 662
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 694
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1363910256
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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