BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060191.seq
(565 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces ... 34 0.017
SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|ch... 27 1.4
SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 26 4.4
SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 25 7.7
>SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 227
Score = 33.9 bits (74), Expect = 0.017
Identities = 24/77 (31%), Positives = 34/77 (44%)
Frame = -3
Query: 371 SSSSRYQINIEFQLCMTRFGNAIFISFLLVDNNTSVITTAICILSLASGSTAFLSTTNVV 192
S +S Q+ +C+ IF+SF DN ITT A S + T+NV+
Sbjct: 139 SRASFSQVASFMGVCVWSVPIGIFVSFTAADNTLPTITTPSSSSPDAYSSGSSRRTSNVL 198
Query: 191 ELAVLHLGVIITFSSTS 141
A LG+ + S TS
Sbjct: 199 RQAYKKLGIFVERSLTS 215
>SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 402
Score = 27.5 bits (58), Expect = 1.4
Identities = 18/69 (26%), Positives = 34/69 (49%), Gaps = 1/69 (1%)
Frame = +1
Query: 163 MTPKCKTANSTTLVVERKAVEPEASDKIHIAVVITL-VLLSTSKKLMKMAFPNLVMHNWN 339
M+PK K+ N T +V + +K H ++ L +++ T KL K+ N+V+ +
Sbjct: 1 MSPKSKSTNKTYTIVIKLGTSSICDEKTHEPLISNLSLIVETVVKLRKLGH-NVVLVSSG 59
Query: 340 SMFIWYREL 366
+ + R L
Sbjct: 60 GIAMGLRRL 68
>SPBC119.02 |ubc4||ubiquitin conjugating enzyme
Ubc4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 147
Score = 25.8 bits (54), Expect = 4.4
Identities = 10/29 (34%), Positives = 13/29 (44%)
Frame = +3
Query: 405 WFKPQMTPNERPYEAQAFFRELDSPXDFP 491
W M P + PY FF + P D+P
Sbjct: 33 WQATIMGPADSPYAGGVFFLSIHFPTDYP 61
>SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1010
Score = 25.0 bits (52), Expect = 7.7
Identities = 13/34 (38%), Positives = 17/34 (50%)
Frame = -3
Query: 233 ASGSTAFLSTTNVVELAVLHLGVIITFSSTSAMV 132
A GS F+S + LA H GV TF + +V
Sbjct: 444 AEGSNNFISDIFFLNLAFHHYGVNATFKALEQLV 477
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,112,591
Number of Sequences: 5004
Number of extensions: 43172
Number of successful extensions: 113
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 111
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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