BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060191.seq (565 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces ... 34 0.017 SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|ch... 27 1.4 SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccha... 26 4.4 SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomy... 25 7.7 >SPCC1795.10c |||Sed5 Vesicle Protein Svp26 |Schizosaccharomyces pombe|chr 3|||Manual Length = 227 Score = 33.9 bits (74), Expect = 0.017 Identities = 24/77 (31%), Positives = 34/77 (44%) Frame = -3 Query: 371 SSSSRYQINIEFQLCMTRFGNAIFISFLLVDNNTSVITTAICILSLASGSTAFLSTTNVV 192 S +S Q+ +C+ IF+SF DN ITT A S + T+NV+ Sbjct: 139 SRASFSQVASFMGVCVWSVPIGIFVSFTAADNTLPTITTPSSSSPDAYSSGSSRRTSNVL 198 Query: 191 ELAVLHLGVIITFSSTS 141 A LG+ + S TS Sbjct: 199 RQAYKKLGIFVERSLTS 215 >SPAC17H9.13c |||glutamate 5-kinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 402 Score = 27.5 bits (58), Expect = 1.4 Identities = 18/69 (26%), Positives = 34/69 (49%), Gaps = 1/69 (1%) Frame = +1 Query: 163 MTPKCKTANSTTLVVERKAVEPEASDKIHIAVVITL-VLLSTSKKLMKMAFPNLVMHNWN 339 M+PK K+ N T +V + +K H ++ L +++ T KL K+ N+V+ + Sbjct: 1 MSPKSKSTNKTYTIVIKLGTSSICDEKTHEPLISNLSLIVETVVKLRKLGH-NVVLVSSG 59 Query: 340 SMFIWYREL 366 + + R L Sbjct: 60 GIAMGLRRL 68 >SPBC119.02 |ubc4||ubiquitin conjugating enzyme Ubc4|Schizosaccharomyces pombe|chr 2|||Manual Length = 147 Score = 25.8 bits (54), Expect = 4.4 Identities = 10/29 (34%), Positives = 13/29 (44%) Frame = +3 Query: 405 WFKPQMTPNERPYEAQAFFRELDSPXDFP 491 W M P + PY FF + P D+P Sbjct: 33 WQATIMGPADSPYAGGVFFLSIHFPTDYP 61 >SPAC20H4.10 |ufd2||ubiquitin-protein ligase E4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1010 Score = 25.0 bits (52), Expect = 7.7 Identities = 13/34 (38%), Positives = 17/34 (50%) Frame = -3 Query: 233 ASGSTAFLSTTNVVELAVLHLGVIITFSSTSAMV 132 A GS F+S + LA H GV TF + +V Sbjct: 444 AEGSNNFISDIFFLNLAFHHYGVNATFKALEQLV 477 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,112,591 Number of Sequences: 5004 Number of extensions: 43172 Number of successful extensions: 113 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 111 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 113 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 238029836 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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