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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060190.seq
         (643 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei...    76   2e-14
At2g44230.1 68415.m05504 expressed protein                             29   2.0  
At5g27270.1 68418.m03254 pentatricopeptide (PPR) repeat-containi...    29   2.6  
At3g48950.1 68416.m05347 glycoside hydrolase family 28 protein /...    28   6.1  

>At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein
           similar to SP|P12048 Bifunctional purine biosynthesis
           protein purH [Includes:
           Phosphoribosylaminoimidazolecarboxamide
           formyltransferase (EC 2.1.2.3) (AICAR transformylase);
           IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP
           synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam
           profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142:
           MGS-like domain
          Length = 596

 Score = 75.8 bits (178), Expect = 2e-14
 Identities = 50/142 (35%), Positives = 73/142 (51%), Gaps = 5/142 (3%)
 Frame = +1

Query: 34  QNMASNG-KLALLSVSDKTGLLSLAKSLSECGLQLIASGGTATGASERRPHSSRC--VGH 204
           Q+  S+G K AL+S+SDK  L SL   L E G  ++++GGTA+         ++   + H
Sbjct: 64  QSSGSSGEKQALISLSDKRDLASLGNGLQELGYTIVSTGGTASTLENAGVSVTKVEKLTH 123

Query: 205 HESTGDARRSGENFTSRVHAGILARLS-DSDQEDMKRQKYEMISVVVCNLYPFVQTVSKP 381
                D R   +     +H GILAR   +   E +         VVV NLYPF + V+ P
Sbjct: 124 FPEMLDGR--VKTLHPNIHGGILARRDVEHHMEALNEHGIGTFDVVVVNLYPFYEKVTAP 181

Query: 382 -DVTVXDAVENIDIGGVTLLRA 444
             ++  D +ENIDIGG  ++RA
Sbjct: 182 GGISFEDGIENIDIGGPAMIRA 203



 Score = 42.7 bits (96), Expect = 2e-04
 Identities = 19/30 (63%), Positives = 22/30 (73%)
 Frame = +2

Query: 164 LRNAGLTVQDVSDITRAPEMLGGRVKTLHP 253
           L NAG++V  V  +T  PEML GRVKTLHP
Sbjct: 108 LENAGVSVTKVEKLTHFPEMLDGRVKTLHP 137


>At2g44230.1 68415.m05504 expressed protein
          Length = 542

 Score = 29.5 bits (63), Expect = 2.0
 Identities = 14/43 (32%), Positives = 22/43 (51%)
 Frame = -1

Query: 337 PRLSSRTSDVSCLPGQSRIIELRSQHVPWM*SFHPTSEHLRCS 209
           P L +   D SC+P + +I  L   + PW+  FH   ++L  S
Sbjct: 220 PCLKNNNFDFSCMPSKPQIDALFQTYAPWI-YFHKDEKYLPSS 261


>At5g27270.1 68418.m03254 pentatricopeptide (PPR) repeat-containing
            protein contains Pfam profile PF01535: PPR repeat
          Length = 1038

 Score = 29.1 bits (62), Expect = 2.6
 Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 2/58 (3%)
 Frame = +3

Query: 168  GTPASQF--KMCRTSREHRRCSEVG*KLYIQGTCWDLSSIIRL*PGRHETSEVRDDKR 335
            GTP+     K+C TSR H    E+   +   G C DLS+ + L     E+S+  + ++
Sbjct: 878  GTPSYNMMVKICATSRLHHEVDELLQAMERNGRCTDLSTYLTLIQVYAESSQFAEAEK 935


>At3g48950.1 68416.m05347 glycoside hydrolase family 28 protein /
           polygalacturonase (pectinase) family protein weak
           similarity to SP|P27644 Polygalacturonase (EC 3.2.1.15)
           (Pectinase) {Agrobacterium tumefaciens}; contains
           PF00295: Glycosyl hydrolases family 28
          Length = 469

 Score = 27.9 bits (59), Expect = 6.1
 Identities = 14/44 (31%), Positives = 21/44 (47%)
 Frame = +3

Query: 57  TSSSQRFRQDGSTLVSKEPVGMWPAVDCQWRYRHGRFGTPASQF 188
           TS    F Q G+T+++ +    WP +     Y  GR GT   +F
Sbjct: 97  TSHFTLFIQRGATILASQDESEWPVIAPLPSYGKGRDGTGTGRF 140


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,886,073
Number of Sequences: 28952
Number of extensions: 248470
Number of successful extensions: 741
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 739
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1324661040
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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