BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060187.seq (631 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 ... 94 6e-20 At4g21230.1 68417.m03070 protein kinase family protein contains ... 29 1.9 At3g56450.1 68416.m06278 alpha-soluble NSF attachment protein 1 ... 29 2.5 At5g41390.1 68418.m05029 hypothetical protein contains 1 predict... 28 4.4 At3g08820.1 68416.m01024 pentatricopeptide (PPR) repeat-containi... 28 4.4 At3g60920.1 68416.m06815 beige/BEACH domain-containing protein c... 28 5.9 At3g23020.1 68416.m02902 pentatricopeptide (PPR) repeat-containi... 28 5.9 >At3g56190.1 68416.m06245 alpha-soluble NSF attachment protein 2 / alpha-SNAP2 / ASNAP2 identical to alpha-soluble NSF attachment protein 2 / alpha-SNAP2 SP:Q9SPE6 from [Arabidopsis thaliana] Length = 289 Score = 94.3 bits (224), Expect = 6e-20 Identities = 48/124 (38%), Positives = 68/124 (54%) Frame = +2 Query: 251 QVAKKWPQAGQAFCNAAQLHLKAGVRHDAATNFVDASNCYKKCDANEAVSCLLKAIEIYT 430 ++AK W QAG+A+ A HLK+ +HDAA + +A+ CYKK D NEA SCL +A+ I+ Sbjct: 44 KLAKSWDQAGKAYLKLADCHLKSDSKHDAANAYAEAAKCYKKVDTNEAASCLERAVNIFC 103 Query: 431 DMGRFTVAAKQHQNIAECTRRSALXXXXXXXXXXXXXXXXXXXXXXXXXNKCMLKLAHYA 610 ++GR +AA+ ++ IAE S N+C LK+A YA Sbjct: 104 EIGRLNMAARYYKEIAE-YYESDQKFEQAIAYFEKAAEFFQNEEVTTSANQCNLKVAQYA 162 Query: 611 AQLE 622 AQLE Sbjct: 163 AQLE 166 >At4g21230.1 68417.m03070 protein kinase family protein contains Pfam domain, PF00069: Protein kinase domain Length = 642 Score = 29.5 bits (63), Expect = 1.9 Identities = 16/38 (42%), Positives = 23/38 (60%), Gaps = 1/38 (2%) Frame = -1 Query: 370 VTVRSI-YKIGGSIMANTSFQVELCCITESLPCLRPLL 260 VTV +I Y GG+ +NTS+ + L + SLP L P + Sbjct: 28 VTVHTICYYDGGNFTSNTSYSLNLNRLISSLPDLTPTI 65 >At3g56450.1 68416.m06278 alpha-soluble NSF attachment protein 1 / alpha-SNAP1 (ASNAP1) identical to alpha-soluble NSF attachment protein 1 (Alpha-SNAP1) (N- ethylmaleimide-sensitive factor attachment protein, alpha 1) (Swiss-Prot:Q9LXZ5) [Arabidopsis thaliana] Length = 381 Score = 29.1 bits (62), Expect = 2.5 Identities = 12/23 (52%), Positives = 15/23 (65%) Frame = +2 Query: 251 QVAKKWPQAGQAFCNAAQLHLKA 319 ++AK W QAG A+ A HLKA Sbjct: 132 KLAKSWDQAGIAYLKLADCHLKA 154 >At5g41390.1 68418.m05029 hypothetical protein contains 1 predicted transmembrane domain; Length = 297 Score = 28.3 bits (60), Expect = 4.4 Identities = 12/32 (37%), Positives = 16/32 (50%) Frame = +2 Query: 353 DASNCYKKCDANEAVSCLLKAIEIYTDMGRFT 448 D C+ C VS LL+ +Y DM R+T Sbjct: 32 DTPYCFFSCLCGPCVSYLLRKRALYNDMSRYT 63 >At3g08820.1 68416.m01024 pentatricopeptide (PPR) repeat-containing protein contains Pfam profile PF01535: PPR repeat Length = 685 Score = 28.3 bits (60), Expect = 4.4 Identities = 17/59 (28%), Positives = 25/59 (42%) Frame = +2 Query: 305 LHLKAGVRHDAATNFVDASNCYKKCDANEAVSCLLKAIEIYTDMGRFTVAAKQHQNIAE 481 L LKA R + +D + KC N V+ + + IY+ GR A K I + Sbjct: 116 LVLKACTRASSRKLGIDLHSLVVKCGFNHDVAAMTSLLSIYSGSGRLNDAHKLFDEIPD 174 >At3g60920.1 68416.m06815 beige/BEACH domain-containing protein contains Pfam PF02138: Beige/BEACH domain; similar to LBA isoform gamma (GI:10257405) [Mus musculus]; similar to beige-like protein (CDC4L) - Homo sapiens; similar to Neurobeachin: kinase A-anchoring, beige/Chediak-higashi protein homolog implicated in neuronal membrane traffic (AKAP550) (GI:11863541) [Drosophila melanogaster]. Length = 1928 Score = 27.9 bits (59), Expect = 5.9 Identities = 10/15 (66%), Positives = 12/15 (80%) Frame = -3 Query: 335 HHGEHQLSSGAVLHY 291 H+G H S+GAVLHY Sbjct: 1755 HYGSHYSSAGAVLHY 1769 >At3g23020.1 68416.m02902 pentatricopeptide (PPR) repeat-containing protein low similarity to leaf protein [Ipomoea nil] GI:3107905; contains Pfam profile PF01535: PPR repeat Length = 842 Score = 27.9 bits (59), Expect = 5.9 Identities = 17/63 (26%), Positives = 30/63 (47%), Gaps = 1/63 (1%) Frame = -1 Query: 496 TPSRTLRNVLVLFGSHSETTHIG-VDLDRFQETGHGLVSITFLVTVRSIYKIGGSIMANT 320 TP + N LV + ++ H G L++ +ETG+ I + + S K+G MA Sbjct: 537 TPDKCTYNTLVQILASADMPHKGRCYLEKMRETGYVSDCIPYCAVISSFVKLGQLNMAEE 596 Query: 319 SFQ 311 ++ Sbjct: 597 VYK 599 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,722,844 Number of Sequences: 28952 Number of extensions: 212508 Number of successful extensions: 538 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 527 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 538 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1285411824 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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