BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060186.seq (566 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch... 48 7e-07 SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|S... 29 0.36 SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|ch... 29 0.48 SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|... 25 7.7 SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S... 25 7.7 >SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr 1|||Manual Length = 219 Score = 48.4 bits (110), Expect = 7e-07 Identities = 24/74 (32%), Positives = 35/74 (47%) Frame = +3 Query: 297 LRSTIMREFXXXXXXXXXXXXXALTVQFXSGXFMEKYDPTXEDFYRKGIXVDNSPCV*KF 476 +RST +RE+ ALT+Q F+++YDPT ED YRK +D + Sbjct: 1 MRSTYLREYKLVVVGDGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKKCEIDGEGALLDV 60 Query: 477 WTPLGTEQXCLMRD 518 G E+ MR+ Sbjct: 61 LDTAGQEEYSAMRE 74 >SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|Schizosaccharomyces pombe|chr 3|||Manual Length = 638 Score = 29.5 bits (63), Expect = 0.36 Identities = 17/43 (39%), Positives = 22/43 (51%), Gaps = 1/43 (2%) Frame = -3 Query: 144 FHVN*NNSNCIALITLKTNA-EHNFHSTDVINTSLVPHEQQVG 19 FH + NN C+AL T + F++ D TSL H QVG Sbjct: 416 FHTS-NNRGCLALFDASTASFVQKFNTADEAFTSLYMHPSQVG 457 >SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|chr 2|||Manual Length = 185 Score = 29.1 bits (62), Expect = 0.48 Identities = 12/27 (44%), Positives = 17/27 (62%) Frame = +3 Query: 363 ALTVQFXSGXFMEKYDPTXEDFYRKGI 443 +LTVQ+ F+E Y PT E+ + K I Sbjct: 21 SLTVQYVENHFVESYYPTIENTFSKNI 47 >SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr 2|||Manual Length = 743 Score = 25.0 bits (52), Expect = 7.7 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -3 Query: 372 QSTHSFXPHPNPTRPL*IRA 313 Q THS P PN + PL +R+ Sbjct: 589 QQTHSSRPTPNASSPLDVRS 608 >SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 1052 Score = 25.0 bits (52), Expect = 7.7 Identities = 17/61 (27%), Positives = 28/61 (45%), Gaps = 3/61 (4%) Frame = -1 Query: 338 QHDHFKFAHYCAP*ITXASLPH---NVIETYHTVRYKMSLLCWFMTHSDAITELHLLKLI 168 Q D +A AP I ++L N + + +Y + + WF+ HS I+ L+ I Sbjct: 141 QRDSIVYAADSAPIIQVSTLETTDPNKLWKVSSKQYDLREVTWFLDHSSLISRLYFDVKI 200 Query: 167 D 165 D Sbjct: 201 D 201 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,211,353 Number of Sequences: 5004 Number of extensions: 39247 Number of successful extensions: 76 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 71 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 76 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 240047038 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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