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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060186.seq
         (566 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch...    48   7e-07
SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|S...    29   0.36 
SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|ch...    29   0.48 
SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|...    25   7.7  
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S...    25   7.7  

>SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 219

 Score = 48.4 bits (110), Expect = 7e-07
 Identities = 24/74 (32%), Positives = 35/74 (47%)
 Frame = +3

Query: 297 LRSTIMREFXXXXXXXXXXXXXALTVQFXSGXFMEKYDPTXEDFYRKGIXVDNSPCV*KF 476
           +RST +RE+             ALT+Q     F+++YDPT ED YRK   +D    +   
Sbjct: 1   MRSTYLREYKLVVVGDGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKKCEIDGEGALLDV 60

Query: 477 WTPLGTEQXCLMRD 518
               G E+   MR+
Sbjct: 61  LDTAGQEEYSAMRE 74


>SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor
           Raf1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 638

 Score = 29.5 bits (63), Expect = 0.36
 Identities = 17/43 (39%), Positives = 22/43 (51%), Gaps = 1/43 (2%)
 Frame = -3

Query: 144 FHVN*NNSNCIALITLKTNA-EHNFHSTDVINTSLVPHEQQVG 19
           FH + NN  C+AL    T +    F++ D   TSL  H  QVG
Sbjct: 416 FHTS-NNRGCLALFDASTASFVQKFNTADEAFTSLYMHPSQVG 457


>SPBC428.16c |rhb1||Rheb GTPase Rhb1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 185

 Score = 29.1 bits (62), Expect = 0.48
 Identities = 12/27 (44%), Positives = 17/27 (62%)
 Frame = +3

Query: 363 ALTVQFXSGXFMEKYDPTXEDFYRKGI 443
           +LTVQ+    F+E Y PT E+ + K I
Sbjct: 21  SLTVQYVENHFVESYYPTIENTFSKNI 47


>SPBC1289.10c |||transcription factor |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 743

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 10/20 (50%), Positives = 13/20 (65%)
 Frame = -3

Query: 372 QSTHSFXPHPNPTRPL*IRA 313
           Q THS  P PN + PL +R+
Sbjct: 589 QQTHSSRPTPNASSPLDVRS 608


>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1052

 Score = 25.0 bits (52), Expect = 7.7
 Identities = 17/61 (27%), Positives = 28/61 (45%), Gaps = 3/61 (4%)
 Frame = -1

Query: 338 QHDHFKFAHYCAP*ITXASLPH---NVIETYHTVRYKMSLLCWFMTHSDAITELHLLKLI 168
           Q D   +A   AP I  ++L     N +    + +Y +  + WF+ HS  I+ L+    I
Sbjct: 141 QRDSIVYAADSAPIIQVSTLETTDPNKLWKVSSKQYDLREVTWFLDHSSLISRLYFDVKI 200

Query: 167 D 165
           D
Sbjct: 201 D 201


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,211,353
Number of Sequences: 5004
Number of extensions: 39247
Number of successful extensions: 76
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 71
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 76
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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