Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060183.seq
         (676 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10 |Schizo...    59   7e-10
SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|c...    30   0.35 
SPAC694.06c |mrc1||mediator of replication checkpoint 1 |Schizos...    28   1.4  
SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces ...    27   2.5  
SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces po...    27   2.5  
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces...    27   3.3  
SPCC18B5.03 |wee1||dual specificity protein kinase Wee1|Schizosa...    26   4.3  
SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyce...    25   7.6  
SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15 |Sc...    25   7.6  
SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase I|Schizosacchar...    25   10.0 
SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|...    25   10.0 

>SPBC215.09c |erg10||acetyl-CoA C-acetyltransferase Erg10
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 395

 Score = 58.8 bits (136), Expect = 7e-10
 Identities = 32/80 (40%), Positives = 46/80 (57%)
 Frame = +2

Query: 260 GQVISXSQTDGIYTPRHAALKAGIPQEKPVLGINRLCGSGFQSIVNSAQDILTGAAKISV 439
           G V+S +   G    R  AL AG+P+      +N++C SG ++ +  AQ I+TG A+I V
Sbjct: 56  GNVVSANL--GQNPARQCALGAGLPRSIVCTTVNKVCASGMKATILGAQTIMTGNAEIVV 113

Query: 440 AXGVENMSXAPFAVRNVRFG 499
           A G E+MS AP+     RFG
Sbjct: 114 AGGTESMSNAPYYAPKNRFG 133



 Score = 50.4 bits (115), Expect = 2e-07
 Identities = 22/48 (45%), Positives = 32/48 (66%)
 Frame = +3

Query: 111 IFIVGAKRTPFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSM 254
           ++IV A RTP G+FGG F +  AT+L +IA  GAL+   + P+ VD +
Sbjct: 6   VYIVSAVRTPMGSFGGSFASLPATKLGSIAIKGALERVNIKPSDVDEV 53



 Score = 25.4 bits (53), Expect = 7.6
 Identities = 10/12 (83%), Positives = 11/12 (91%)
 Frame = +3

Query: 546 GLSDSYCGLPMG 581
           GLSD+Y GLPMG
Sbjct: 149 GLSDAYDGLPMG 160


>SPBC1683.03c |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 519

 Score = 29.9 bits (64), Expect = 0.35
 Identities = 14/30 (46%), Positives = 16/30 (53%)
 Frame = +2

Query: 497 GTALAPTTPSKDTLWARTLGLILRTPHGHD 586
           GT L  T P   T WA+T   I+ TP G D
Sbjct: 384 GTILIATAPIHQTYWAQTFVSIIVTPWGMD 413


>SPAC694.06c |mrc1||mediator of replication checkpoint 1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1019

 Score = 27.9 bits (59), Expect = 1.4
 Identities = 16/59 (27%), Positives = 28/59 (47%)
 Frame = +3

Query: 168 NTSATELQTIATVGALKEAGVSPAQVDSMSXAKSSLXHKLTESTRHVTRRSKLVFLKRS 344
           NTS+T+   + ++   +     P Q+DS+   K     ++ E  R  +RR    FL R+
Sbjct: 630 NTSSTQPSQVDSLVPTQLDSTIPTQIDSVQRNKDQDDEEILEERRE-SRRDSKTFLSRT 687


>SPAC222.09 |seb1||RNA-binding protein Seb1 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 14/42 (33%), Positives = 20/42 (47%)
 Frame = +3

Query: 138 PFGTFGGVFRNTSATELQTIATVGALKEAGVSPAQVDSMSXA 263
           P G  G    + + T+ Q I  +  L    V PAQ+DS+  A
Sbjct: 270 PSGPMGMKTGSVNDTQSQQITLMNVLASQNVPPAQIDSIMKA 311


>SPAC2F3.10 |||GARP complex subunit Vps54 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 949

 Score = 27.1 bits (57), Expect = 2.5
 Identities = 11/52 (21%), Positives = 27/52 (51%), Gaps = 1/52 (1%)
 Frame = -1

Query: 433 DLGCAGQNVLSAVHDRLE-TRAA*SVDAEHRLLLRNTSFERRVTWRVDSVSL 281
           D       +   V + ++ T+ + + ++   LLL N+  + +++W +D+V L
Sbjct: 177 DFNLDDPQIFDVVSEHIDITQTSDAPNSNRNLLLNNSMLQEKISWYLDTVEL 228


>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1009

 Score = 26.6 bits (56), Expect = 3.3
 Identities = 8/19 (42%), Positives = 13/19 (68%)
 Frame = +2

Query: 530 DTLWARTLGLILRTPHGHD 586
           +T W +  G++L  PHG+D
Sbjct: 758 ETKWLQRTGIVLSLPHGYD 776


>SPCC18B5.03 |wee1||dual specificity protein kinase
           Wee1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 877

 Score = 26.2 bits (55), Expect = 4.3
 Identities = 16/69 (23%), Positives = 30/69 (43%), Gaps = 1/69 (1%)
 Frame = +1

Query: 403 SGHFDRRSQDLSSGGSREHVAX-ALRGQKREVRHGSGTNYAFERHPLGPDSRTHTADSPW 579
           S  +D  +   S+   + + +  +L G +++       ++A   HPL P S TH+     
Sbjct: 36  SSLYDANNSTSSTSSQKPNTSFTSLFGPRKQTTSSPSFSHAAPLHPLSPPSFTHSQPQIQ 95

Query: 580 A*PAEKARS 606
           A P  +  S
Sbjct: 96  AQPVPRRPS 104


>SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 1112

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 12/39 (30%), Positives = 19/39 (48%)
 Frame = -1

Query: 589 RVMPMGSPQYESESPGPEGVFRRRSWCQSRAEPHVSDRE 473
           RV P+ + +   E PG  G+F  + W Q  +  H S  +
Sbjct: 856 RVGPVLALEILHEFPGDTGLFEFKKWFQRLSTGHASKND 894


>SPBC146.01 |med15|SPBP35G2.15|mediator complex subunit Med15
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1063

 Score = 25.4 bits (53), Expect = 7.6
 Identities = 12/46 (26%), Positives = 23/46 (50%)
 Frame = -1

Query: 184 SVADVLRKTPPKVPNGVLLAPTMKIPLTEADMIQNYTYPVNVELKY 47
           SV+  +R     +PN     P    P  +A+ + N ++PV+ +L +
Sbjct: 755 SVSSNVRPINGSIPNAQPSVPGQAQPAAKANSVGNTSFPVDSKLAF 800


>SPAC13G6.12c |chs1|SPAC24B11.01c|chitin synthase
           I|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 859

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 17/68 (25%), Positives = 29/68 (42%)
 Frame = +1

Query: 424 SQDLSSGGSREHVAXALRGQKREVRHGSGTNYAFERHPLGPDSRTHTADSPWA*PAEKAR 603
           + D+S G   +H      G  R ++ G+    A   +    D++   A    A P+ +  
Sbjct: 715 THDVSWGTKGDHFTNNDLGVARLLQKGADVEIAIPTNQSDIDAKYEDAVKLLASPSLEFN 774

Query: 604 SPVRNHQE 627
           SP+ NH E
Sbjct: 775 SPIINHGE 782


>SPCC777.04 |||amino acid transporter |Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 521

 Score = 25.0 bits (52), Expect = 10.0
 Identities = 6/13 (46%), Positives = 12/13 (92%)
 Frame = +2

Query: 62  VNWIGIILNHVCF 100
           +NWI I+L+++C+
Sbjct: 421 INWINILLSYICY 433


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,667,964
Number of Sequences: 5004
Number of extensions: 50905
Number of successful extensions: 147
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 147
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 309878492
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)