Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060174.seq
(662 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 136 3e-33
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 85 9e-18
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 84 2e-17
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 67 2e-12
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 26 4.2
SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|c... 26 4.2
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 26 5.6
SPBC646.12c |gap1|src1, sar1|GTPase activating protein Gap1|Schi... 25 7.4
SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3 |Schi... 25 7.4
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 7.4
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 136 bits (329), Expect = 3e-33
Identities = 66/121 (54%), Positives = 78/121 (64%)
Frame = +1
Query: 256 AILXDLEPGTMDSVRSGPFGQIXRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRK 435
A+L DLEPGTMD+V+SG FG + RPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVVR+
Sbjct: 63 AVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRR 122
Query: 436 EAESCDCLQGFQLTHXXXXXXXXXXXXXXXPKSEKSTLTE**THXQLXHXPKVSGTVVXP 615
EAE+CD LQGFQLTH K + + PK S TVV P
Sbjct: 123 EAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDTVVEP 182
Query: 616 F 618
+
Sbjct: 183 Y 183
Score = 103 bits (247), Expect = 2e-23
Identities = 43/60 (71%), Positives = 50/60 (83%)
Frame = +2
Query: 71 MREIVHIQAGQCGNQIGAKFWEIISDEHGIXPTGAYHGDSDLQLERINVYYNEASGGKYV 250
MREIVHIQAGQCGNQ+GA FW I+DEHG+ G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 85.0 bits (201), Expect = 9e-18
Identities = 35/75 (46%), Positives = 52/75 (69%)
Frame = +1
Query: 256 AILXDLEPGTMDSVRSGPFGQIXRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRK 435
+I DLEP +D VR+GP+ + P+ V G+ A NN+A+GHYT G E++DSVL+ +R+
Sbjct: 69 SIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRR 128
Query: 436 EAESCDCLQGFQLTH 480
A++C LQGF + H
Sbjct: 129 MADNCSGLQGFLVFH 143
Score = 44.4 bits (100), Expect = 1e-05
Identities = 22/56 (39%), Positives = 31/56 (55%)
Frame = +2
Query: 71 MREIVHIQAGQCGNQIGAKFWEIISDEHGIXPTGAYHGDSDLQLERINVYYNEASG 238
MRE++ + GQ G QIG WE+ EHGI P G +S ++ + N Y N+ G
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENS--EVHKNNSYLNDGFG 54
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 83.8 bits (198), Expect = 2e-17
Identities = 35/75 (46%), Positives = 50/75 (66%)
Frame = +1
Query: 256 AILXDLEPGTMDSVRSGPFGQIXRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRK 435
+I DLEP +D VR+GP+ + P+ + G+ A NN+A+GHYT G ELVD V D +R+
Sbjct: 65 SIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRR 124
Query: 436 EAESCDCLQGFQLTH 480
A++C LQGF + H
Sbjct: 125 IADNCSGLQGFLVFH 139
Score = 50.0 bits (114), Expect = 3e-07
Identities = 25/62 (40%), Positives = 34/62 (54%), Gaps = 2/62 (3%)
Frame = +2
Query: 71 MREIVHIQAGQCGNQIGAKFWEIISDEHGIXPTGAYHGDSDLQLE--RINVYYNEASGGK 244
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 245 YV 250
YV
Sbjct: 61 YV 62
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 67.3 bits (157), Expect = 2e-12
Identities = 29/77 (37%), Positives = 49/77 (63%), Gaps = 2/77 (2%)
Frame = +1
Query: 256 AILXDLEPGTMDSVRSGPFGQIXRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDVV 429
AIL DLEP ++++ S +G + P+N + ++ GAGNNWA G Y+ + + ++D++
Sbjct: 64 AILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMI 122
Query: 430 RKEAESCDCLQGFQLTH 480
+EA+ D L+GF L H
Sbjct: 123 DREADGSDSLEGFSLLH 139
Score = 60.1 bits (139), Expect = 3e-10
Identities = 24/59 (40%), Positives = 40/59 (67%)
Frame = +2
Query: 74 REIVHIQAGQCGNQIGAKFWEIISDEHGIXPTGAYHGDSDLQLERINVYYNEASGGKYV 250
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYI 61
Score = 28.7 bits (61), Expect = 0.79
Identities = 9/25 (36%), Positives = 17/25 (68%)
Frame = +3
Query: 510 GTLLISKIREEYPDRIMNTYXVXPS 584
G+ L+ ++ + YP +I+ TY V P+
Sbjct: 150 GSFLLERLNDRYPKKIIQTYSVFPN 174
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 26.2 bits (55), Expect = 4.2
Identities = 12/30 (40%), Positives = 19/30 (63%)
Frame = +1
Query: 373 AKGHYTEGAELVDSVLDVVRKEAESCDCLQ 462
A+GH G ELV + D +RK++E+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPBC15D4.03 |slm9||hira protein Slm9|Schizosaccharomyces pombe|chr
2|||Manual
Length = 807
Score = 26.2 bits (55), Expect = 4.2
Identities = 16/46 (34%), Positives = 23/46 (50%)
Frame = -1
Query: 446 DSASFRTTSKTESTSSAPSV*CPLAQLLPAPDCPKTKLSGRKICPK 309
++A+ RTTS T+ +PS L P P + S R+ CPK
Sbjct: 390 NAAADRTTSPTQGQPESPS---KSILLRPPPSIASSPESKRRKCPK 432
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.8 bits (54), Expect = 5.6
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +1
Query: 325 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKEAESCDC 456
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPBC646.12c |gap1|src1, sar1|GTPase activating protein
Gap1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 766
Score = 25.4 bits (53), Expect = 7.4
Identities = 15/46 (32%), Positives = 27/46 (58%)
Frame = -2
Query: 223 VVYIDALQLQVRVPMVSTGGVDAVLVGDDLPELSSDLVAALTSLDM 86
++Y+DA L +++ + G A V DLP L +D V++L S+ +
Sbjct: 511 ILYVDAKTLFIQLLRLLPSGHPATRVPLDLP-LIADSVSSLKSMSL 555
>SPBC11C11.11c ||SPBC3B8.12|ATP-dependent DNA helicase Irc3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 606
Score = 25.4 bits (53), Expect = 7.4
Identities = 17/39 (43%), Positives = 22/39 (56%)
Frame = -3
Query: 411 IDELSTLRVVSLGPVVTGAGLSEDEVVRTXDLSERSRAD 295
+D +LR VSL T AGLS DEV + S+ S A+
Sbjct: 369 LDFCDSLRRVSLHVDPTLAGLSPDEVENFYNKSKNSLAN 407
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 7.4
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -1
Query: 362 PAPDCPKTKLSGRKICPKGPERTESMVPGSKS 267
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,162,935
Number of Sequences: 5004
Number of extensions: 36288
Number of successful extensions: 108
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 106
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 301829700
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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