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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060169.seq
         (665 letters)

Database: bee 
           438 sequences; 146,343 total letters

Searching......................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholi...    27   0.21 
DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholi...    27   0.21 
DQ667194-1|ABG75746.1|  391|Apis mellifera cys-loop ligand-gated...    22   6.0  
DQ325124-1|ABD14138.1|  179|Apis mellifera complementary sex det...    22   6.0  
DQ325123-1|ABD14137.1|  179|Apis mellifera complementary sex det...    22   6.0  
DQ325122-1|ABD14136.1|  179|Apis mellifera complementary sex det...    22   6.0  
DQ026031-1|AAY87890.1|  601|Apis mellifera nicotinic acetylcholi...    21   8.0  

>DQ026034-1|AAY87893.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 26.6 bits (56), Expect = 0.21
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +3

Query: 273 LSMRCEEWLFRFIQIMEEVLSQILQQDPHFVNSEFPPPWTLHEATQCI 416
           L   CE+        +EEV S +L+  P F +S  PP   +H++  C+
Sbjct: 446 LHTECEDLSVSGEAGIEEVKSPVLRSPPAFSHSRCPP--EIHKSCICV 491


>DQ026033-1|AAY87892.1|  569|Apis mellifera nicotinic acetylcholine
           receptor alpha4subunit protein.
          Length = 569

 Score = 26.6 bits (56), Expect = 0.21
 Identities = 15/48 (31%), Positives = 24/48 (50%)
 Frame = +3

Query: 273 LSMRCEEWLFRFIQIMEEVLSQILQQDPHFVNSEFPPPWTLHEATQCI 416
           L   CE+        +EEV S +L+  P F +S  PP   +H++  C+
Sbjct: 446 LHTECEDLSVSGEAGIEEVKSPVLRSPPAFSHSRCPP--EIHKSCICV 491


>DQ667194-1|ABG75746.1|  391|Apis mellifera cys-loop ligand-gated
           ion channel subunit protein.
          Length = 391

 Score = 21.8 bits (44), Expect = 6.0
 Identities = 12/45 (26%), Positives = 24/45 (53%), Gaps = 4/45 (8%)
 Frame = +3

Query: 252 QTVIEEHLSMRCEEWLFR----FIQIMEEVLSQILQQDPHFVNSE 374
           Q+ IE  L M   +++F     ++ +  E    + Q DP+F+N++
Sbjct: 40  QSWIESRLDM--PDYIFEEGDDYVTLPPEFFDSLWQPDPYFLNAK 82


>DQ325124-1|ABD14138.1|  179|Apis mellifera complementary sex
           determiner protein.
          Length = 179

 Score = 21.8 bits (44), Expect = 6.0
 Identities = 7/26 (26%), Positives = 15/26 (57%)
 Frame = -2

Query: 109 YYYQEHDLK*LHCSCYYNQQYRLSFL 32
           Y Y  ++ K L+C+ Y    Y ++++
Sbjct: 89  YNYNNNNYKKLYCNNYKKLYYNINYI 114


>DQ325123-1|ABD14137.1|  179|Apis mellifera complementary sex
           determiner protein.
          Length = 179

 Score = 21.8 bits (44), Expect = 6.0
 Identities = 7/26 (26%), Positives = 15/26 (57%)
 Frame = -2

Query: 109 YYYQEHDLK*LHCSCYYNQQYRLSFL 32
           Y Y  ++ K L+C+ Y    Y ++++
Sbjct: 89  YNYNNNNYKKLYCNNYKKLYYNINYI 114


>DQ325122-1|ABD14136.1|  179|Apis mellifera complementary sex
           determiner protein.
          Length = 179

 Score = 21.8 bits (44), Expect = 6.0
 Identities = 7/26 (26%), Positives = 15/26 (57%)
 Frame = -2

Query: 109 YYYQEHDLK*LHCSCYYNQQYRLSFL 32
           Y Y  ++ K L+C+ Y    Y ++++
Sbjct: 89  YNYNNNNYKKLYCNNYRKLYYNINYI 114


>DQ026031-1|AAY87890.1|  601|Apis mellifera nicotinic acetylcholine
           receptor alpha1subunit protein.
          Length = 601

 Score = 21.4 bits (43), Expect = 8.0
 Identities = 8/21 (38%), Positives = 12/21 (57%)
 Frame = -1

Query: 452 VYNITAVTKLLYYALRCFV*C 390
           V+NIT   K L+Y +   + C
Sbjct: 227 VFNITLRRKTLFYTVNLIIPC 247


  Database: bee
    Posted date:  Oct 23, 2007  1:17 PM
  Number of letters in database: 146,343
  Number of sequences in database:  438
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 173,190
Number of Sequences: 438
Number of extensions: 3667
Number of successful extensions: 8
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20099475
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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