BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060167.seq
(534 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S... 126 7e-30
At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) str... 126 7e-30
At1g76010.1 68414.m08825 expressed protein 30 0.84
At1g57750.1 68414.m06552 cytochrome P450, putative similar to cy... 29 1.5
At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to S... 29 1.5
At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to S... 29 1.5
At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp... 29 1.9
At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp... 29 1.9
At4g39480.1 68417.m05585 cytochrome P450 family protein contains... 28 3.4
At5g17650.1 68418.m02069 glycine/proline-rich protein glycine/pr... 28 4.5
At3g12430.1 68416.m01548 expressed protein ; expression supporte... 28 4.5
At3g18640.1 68416.m02368 zinc finger protein-related contains si... 27 5.9
At1g80480.1 68414.m09427 PRLI-interacting factor L, putative sim... 27 5.9
At3g04640.1 68416.m00497 glycine-rich protein predicted proteins... 27 7.9
At3g03470.1 68416.m00345 cytochrome P450, putative similar to cy... 27 7.9
At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to... 27 7.9
>At5g02870.1 68418.m00230 60S ribosomal protein L4/L1 (RPL4D) 60S
roibosomal protein L4, Arabidopsis thaliana,
EMBL:CAA79104
Length = 407
Score = 126 bits (305), Expect = 7e-30
Identities = 54/59 (91%), Positives = 56/59 (94%)
Frame = +2
Query: 254 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHRRVN 430
AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWHRRVN
Sbjct: 64 AGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVN 122
Score = 55.6 bits (128), Expect = 2e-08
Identities = 28/58 (48%), Positives = 36/58 (62%), Gaps = 2/58 (3%)
Frame = +1
Query: 85 SVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVTR 252
+ ARPLV+V + T Q LP V AP+RPD+VN VH +S NSRQPY V++
Sbjct: 5 AAARPLVTVQGLDGDMSTDQSTTVTLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVSK 62
Score = 33.1 bits (72), Expect = 0.12
Identities = 13/24 (54%), Positives = 19/24 (79%)
Frame = +1
Query: 460 SVAATGVPALVQARGHILKKISEL 531
++AAT VPALV ARGH ++ + E+
Sbjct: 133 AIAATAVPALVMARGHKIENVPEM 156
>At3g09630.1 68416.m01142 60S ribosomal protein L4/L1 (RPL4A) strong
similarity to 60S ribosomal protein L1 GB:P49691
Length = 406
Score = 126 bits (305), Expect = 7e-30
Identities = 54/59 (91%), Positives = 56/59 (94%)
Frame = +2
Query: 254 AGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGRMFAPTKPWRRWHRRVN 430
AGHQTSAESWGTGRAV+RIPRV GGGTHR+GQ AFGNMCRGGRMFAPTK WRRWHRRVN
Sbjct: 63 AGHQTSAESWGTGRAVSRIPRVPGGGTHRAGQAAFGNMCRGGRMFAPTKIWRRWHRRVN 121
Score = 57.6 bits (133), Expect = 5e-09
Identities = 28/61 (45%), Positives = 40/61 (65%), Gaps = 2/61 (3%)
Frame = +1
Query: 76 MSLSVARPLVSVYSEKSE--TVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCVT 249
M+ + ARPLV++ + + T Q + LP V AP+RPD+VN VH +S NSRQPY V+
Sbjct: 1 MAAAAARPLVTIQTLDGDMSTDQSSTVVLPDVMTAPVRPDIVNFVHAQISNNSRQPYAVS 60
Query: 250 R 252
+
Sbjct: 61 K 61
Score = 33.1 bits (72), Expect = 0.12
Identities = 13/24 (54%), Positives = 19/24 (79%)
Frame = +1
Query: 460 SVAATGVPALVQARGHILKKISEL 531
++AAT VPALV ARGH ++ + E+
Sbjct: 132 AIAATAVPALVMARGHKIENVPEM 155
>At1g76010.1 68414.m08825 expressed protein
Length = 350
Score = 30.3 bits (65), Expect = 0.84
Identities = 18/43 (41%), Positives = 19/43 (44%)
Frame = +2
Query: 248 QGAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRG 376
QG G + G GR R R RGGG R G G F N G
Sbjct: 303 QGRGGYDGPQGRGRGRGRGRGGRGRGGG--RGGDGGFNNRSDG 343
>At1g57750.1 68414.m06552 cytochrome P450, putative similar to
cytochrome P450 GI:4688670 from [Catharanthus roseus]
Length = 497
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/49 (32%), Positives = 29/49 (59%)
Frame = +1
Query: 100 LVSVYSEKSETVQGAAKPLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 246
LV +++ SE+++ PLPF K+P +PD++ H + NS+ C+
Sbjct: 351 LVYLHAALSESMR-LYPPLPFNHKSPAKPDVLPSGH-KVDANSKIVICI 397
>At1g48410.2 68414.m05409 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1050
Score = 29.5 bits (63), Expect = 1.5
Identities = 17/54 (31%), Positives = 25/54 (46%)
Frame = +2
Query: 221 RTRGSPTA*QGAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 382
RT +G+G + + G GR R +GGG H+ G+G +GGR
Sbjct: 6 RTDAPSEGGEGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At1g48410.1 68414.m05408 argonaute protein (AGO1) identical to
SP|O04379 Argonaute protein (AGO1) {Arabidopsis
thaliana}; contains Pfam profiles PF02171: Piwi domain,
PF02170: PAZ domain
Length = 1048
Score = 29.5 bits (63), Expect = 1.5
Identities = 17/54 (31%), Positives = 25/54 (46%)
Frame = +2
Query: 221 RTRGSPTA*QGAGHQTSAESWGTGRAVARIPRVRGGGTHRSGQGAFGNMCRGGR 382
RT +G+G + + G GR R +GGG H+ G+G +GGR
Sbjct: 6 RTDAPSEGGEGSGSREAGPVSGGGRGSQRGGFQQGGGQHQGGRGYTPQPQQGGR 59
>At5g55100.2 68418.m06869 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 844
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -2
Query: 389 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 270
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At5g55100.1 68418.m06868 SWAP (Suppressor-of-White-APricot)/surp
domain-containing protein contains Pfam domain PF01805:
Surp module
Length = 843
Score = 29.1 bits (62), Expect = 1.9
Identities = 12/41 (29%), Positives = 21/41 (51%), Gaps = 1/41 (2%)
Frame = -2
Query: 389 RTYVHHDTCYRRHPDRTY-EYHHHGHAEFGRQHVQYPMIQH 270
R++ H + +H D + E+HHH H R+H ++H
Sbjct: 735 RSHHHRSRKHEKHRDSSDDEHHHHRHRSSRRKHEDSSDVEH 775
>At4g39480.1 68417.m05585 cytochrome P450 family protein contains
Pfam profile: PF00067 cytochrome P450
Length = 989
Score = 28.3 bits (60), Expect = 3.4
Identities = 12/32 (37%), Positives = 20/32 (62%)
Frame = +1
Query: 151 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 246
P+PF K+P +PD++ H + NSR +C+
Sbjct: 384 PVPFNHKSPAKPDVLPSGH-KVKANSRILFCL 414
Score = 27.5 bits (58), Expect = 5.9
Identities = 11/32 (34%), Positives = 20/32 (62%)
Frame = +1
Query: 151 PLPFVFKAPIRPDLVNDVHVSMSKNSRQPYCV 246
P+PF K+P +PD++ H + NS+ +C+
Sbjct: 857 PVPFQHKSPTKPDVLPSGH-KVDANSKILFCL 887
>At5g17650.1 68418.m02069 glycine/proline-rich protein
glycine/proline-rich protein GPRP - Arabidopsis
thaliana, EMBL:X84315
Length = 173
Score = 27.9 bits (59), Expect = 4.5
Identities = 10/24 (41%), Positives = 12/24 (50%)
Frame = -2
Query: 377 HHDTCYRRHPDRTYEYHHHGHAEF 306
HH Y H Y Y +HGH +F
Sbjct: 122 HHHGHYGHHHGHGYGYGYHGHGKF 145
>At3g12430.1 68416.m01548 expressed protein ; expression supported
by MPSS
Length = 238
Score = 27.9 bits (59), Expect = 4.5
Identities = 12/40 (30%), Positives = 22/40 (55%), Gaps = 2/40 (5%)
Frame = -3
Query: 154 GAWLHPAPSHSSLNTPTLKVGLPID--SFRYFSEAIPLKY 41
G W+H SH+ ++ L VG+ + Y+S + P++Y
Sbjct: 38 GQWIHDVLSHNRFSSHPLVVGVGVQWTPSSYYSASSPVRY 77
>At3g18640.1 68416.m02368 zinc finger protein-related contains
similarity to zinc finger proteins (CCCH type)
Length = 676
Score = 27.5 bits (58), Expect = 5.9
Identities = 16/50 (32%), Positives = 26/50 (52%)
Frame = +1
Query: 385 VRPHEALAALAPSRQPPXAESGLGGSVAATGVPALVQARGHILKKISELS 534
V+P++A P GLG S A GVPA+ ++ +++I E+S
Sbjct: 488 VQPNQATTQSNVVSSNPNQLWGLGMSTGAEGVPAVTASKISNVEEIQEVS 537
>At1g80480.1 68414.m09427 PRLI-interacting factor L, putative
similar to PRLI-interacting factor L [Arabidopsis
thaliana] GI:11139268; contains Pfam profile PF02492:
Cobalamin synthesis protein/P47K
Length = 444
Score = 27.5 bits (58), Expect = 5.9
Identities = 9/28 (32%), Positives = 13/28 (46%)
Frame = -2
Query: 377 HHDTCYRRHPDRTYEYHHHGHAEFGRQH 294
HHD + H D +++ HH H H
Sbjct: 311 HHDHDHDHHHDHNHDHDHHHHDGHDHHH 338
>At3g04640.1 68416.m00497 glycine-rich protein predicted proteins,
Arabidopsis thaliana
Length = 159
Score = 27.1 bits (57), Expect = 7.9
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 3/47 (6%)
Frame = +2
Query: 230 GSPTA*QGAGHQTSAESWGT---GRAVARIPRVRGGGTHRSGQGAFG 361
G ++ +G G +S G+ G + IP + GGGTHRSG + G
Sbjct: 93 GGGSSSRGGGGSSSRGGGGSSSRGGGLRPIP-IYGGGTHRSGHHSSG 138
>At3g03470.1 68416.m00345 cytochrome P450, putative similar to
cytochrome P450 89A2 GB:Q42602 [Arabidopsis thaliana]
Length = 511
Score = 27.1 bits (57), Expect = 7.9
Identities = 19/50 (38%), Positives = 21/50 (42%)
Frame = -2
Query: 155 RGLAAPCTVSLFSEYTDTKGRATDRLISLL*RSYTSEIYTNSGDKDHPPS 6
R LA P T + S D L L R+ TSEI S K H PS
Sbjct: 102 RSLALPTTKVITSNQHDIHSSVYGSLWRTLRRNLTSEILQPSRVKAHAPS 151
>At2g05520.1 68415.m00584 glycine-rich protein (GRP) identical to
glycine-rich protein; atGRP (GI:259447) [Arabidopsis
thaliana]
Length = 145
Score = 27.1 bits (57), Expect = 7.9
Identities = 13/32 (40%), Positives = 17/32 (53%)
Frame = +2
Query: 284 GTGRAVARIPRVRGGGTHRSGQGAFGNMCRGG 379
G GR R +GGG + G G+ G+ CR G
Sbjct: 88 GGGRYQGGGGRYQGGGGRQGGGGSGGSYCRHG 119
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,201,038
Number of Sequences: 28952
Number of extensions: 228161
Number of successful extensions: 771
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 707
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 760
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 984125600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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