Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060166.seq
(685 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 195 4e-52
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 195 4e-52
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 187 8e-50
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 186 1e-49
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.7
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.7
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 195 bits (475), Expect = 4e-52
Identities = 105/141 (74%), Positives = 111/141 (78%), Gaps = 1/141 (0%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSEXQI*G-NQEGSILIHQEDWLQPSCC 614
NGQTREHALLAFTLGVKQL NKMDSTEPPYSE + +E S I + + P+
Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGY-NPAAV 131
Query: 615 RFRGPFLDGTGDNMLXPSTKM 677
F P GDNML S+KM
Sbjct: 132 AF-VPISGWHGDNMLEVSSKM 151
Score = 56.4 bits (130), Expect = 2e-10
Identities = 48/140 (34%), Positives = 71/140 (50%), Gaps = 6/140 (4%)
Frame = +1
Query: 223 WVLDKLKAERD---LVSQSILLSGSSKLASTMLPS---LMLLDTEISSRT*SQEPLRLIA 384
WVLDKLKAER+ + ++ +SK T++ + + I+ + + + ++A
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 385 LCSS*LPVPVNSKLVSLRTVKPVSMPCSLSPSVSNSFIVGVTKWIPLNHHTVXPRFEEIK 564
+ ++ + +T + + +L IVGV K RFEEIK
Sbjct: 61 AGTGEFEAGISK---NGQTREHALLAFTLG---VKQLIVGVNKMDSTEPPYSETRFEEIK 114
Query: 565 KEVSSYIKKIGYNPAAVAFV 624
KEVSSYIKKIGYNPAAVAFV
Sbjct: 115 KEVSSYIKKIGYNPAAVAFV 134
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 195 bits (475), Expect = 4e-52
Identities = 105/141 (74%), Positives = 111/141 (78%), Gaps = 1/141 (0%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSEXQI*G-NQEGSILIHQEDWLQPSCC 614
NGQTREHALLAFTLGVKQL NKMDSTEPPYSE + +E S I + + P+
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGY-NPAAV 188
Query: 615 RFRGPFLDGTGDNMLXPSTKM 677
F P GDNML S+KM
Sbjct: 189 AF-VPISGWHGDNMLEVSSKM 208
Score = 174 bits (424), Expect = 6e-46
Identities = 104/197 (52%), Positives = 127/197 (64%), Gaps = 6/197 (3%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAERD---LVSQSILLSGSSKLASTMLPS---LMLLDTEISSRT*SQEPLRLIALCS 393
DKLKAER+ + ++ +SK T++ + + I+ + + + ++A +
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 394 S*LPVPVNSKLVSLRTVKPVSMPCSLSPSVSNSFIVGVTKWIPLNHHTVXPRFEEIKKEV 573
++ + +T + + +L IVGV K RFEEIKKEV
Sbjct: 121 GEFEAGISK---NGQTREHALLAFTLG---VKQLIVGVNKMDSTEPPYSETRFEEIKKEV 174
Query: 574 SSYIKKIGYNPAAVAFV 624
SSYIKKIGYNPAAVAFV
Sbjct: 175 SSYIKKIGYNPAAVAFV 191
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 187 bits (456), Expect = 8e-50
Identities = 101/137 (73%), Positives = 107/137 (78%), Gaps = 1/137 (0%)
Frame = +3
Query: 270 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 449
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 450 REHALLAFTLGVKQLHRRSNKMDSTEPPYSEXQI*G-NQEGSILIHQEDWLQPSCCRFRG 626
REHALLAFTLGVKQL NKMDSTEPPYSE + +E S I + + P+ F
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGY-NPAAVAF-V 118
Query: 627 PFLDGTGDNMLXPSTKM 677
P GDNML S+KM
Sbjct: 119 PISGWHGDNMLEVSSKM 135
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 186 bits (454), Expect = 1e-49
Identities = 100/140 (71%), Positives = 106/140 (75%), Gaps = 1/140 (0%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISK
Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSEXQI*G-NQEGSILIHQEDWLQPSCC 614
NGQTREHALLAFTLGVKQL NKMD T+PPYSE + +E S I + + S
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVA 189
Query: 615 RFRGPFLDGTGDNMLXPSTK 674
P GDNML PS K
Sbjct: 190 FV--PISGWHGDNMLEPSPK 207
Score = 144 bits (349), Expect = 7e-37
Identities = 66/68 (97%), Positives = 67/68 (98%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 309 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.51
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKST 117
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 73 INIVVIGHVDSGKST 117
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 2.7
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -3
Query: 65 FSLPIFG*SRITNCV*Y 15
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.7
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 294 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 395
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 294 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 395
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 198,019
Number of Sequences: 438
Number of extensions: 4077
Number of successful extensions: 30
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 22
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 20830365
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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