Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060166.seq
(685 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha ... 164 6e-41
At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha ... 164 6e-41
At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha ... 164 6e-41
At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha ... 164 6e-41
At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,... 96 2e-20
At5g10630.1 68418.m01231 elongation factor 1-alpha, putative / E... 91 8e-19
At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,... 69 2e-12
At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA) ide... 68 7e-12
At2g45030.1 68415.m05606 mitochondrial elongation factor, putati... 36 0.033
At1g45332.1 68414.m05195 mitochondrial elongation factor, putati... 36 0.033
At4g18330.2 68417.m02719 eukaryotic translation initiation facto... 35 0.058
At4g18330.1 68417.m02718 eukaryotic translation initiation facto... 35 0.058
At1g06220.2 68414.m00656 elongation factor Tu family protein sim... 35 0.058
At1g06220.1 68414.m00655 elongation factor Tu family protein sim... 35 0.058
At1g04170.1 68414.m00407 eukaryotic translation initiation facto... 34 0.076
At2g18720.1 68415.m02180 eukaryotic translation initiation facto... 33 0.13
At5g39900.1 68418.m04839 GTP-binding protein LepA, putative GTP-... 33 0.18
At3g22980.1 68416.m02898 elongation factor Tu family protein sim... 33 0.18
At4g11160.1 68417.m01808 translation initiation factor IF-2, mit... 33 0.23
At1g62750.1 68414.m07082 elongation factor Tu family protein sim... 33 0.23
At5g08650.1 68418.m01029 GTP-binding protein LepA, putative 31 0.71
At3g01360.1 68416.m00057 expressed protein contains Pfam profile... 31 0.94
At1g76825.1 68414.m08940 eukaryotic translation initiation facto... 30 1.6
At1g76810.1 68414.m08938 eukaryotic translation initiation facto... 30 1.6
At1g76720.1 68414.m08929 eukaryotic translation initiation facto... 30 1.6
At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p... 29 2.2
At1g21160.1 68414.m02646 eukaryotic translation initiation facto... 29 2.2
At4g24790.1 68417.m03550 expressed protein ; expression supporte... 29 3.8
At1g78850.1 68414.m09191 curculin-like (mannose-binding) lectin ... 28 6.6
At1g17220.1 68414.m02098 translation initiation factor IF-2, chl... 28 6.6
At3g03380.1 68416.m00336 DegP protease, putative contains simila... 27 8.8
At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha s... 27 8.8
At1g78860.1 68414.m09192 curculin-like (mannose-binding) lectin ... 27 8.8
>At5g60390.1 68418.m07574 elongation factor 1-alpha / EF-1-alpha
identical to SWISS-PROT:P13905 elongation factor 1-alpha
(EF-1-alpha) [Arabidopsis thaliana]
Length = 449
Score = 164 bits (398), Expect = 6e-41
Identities = 77/95 (81%), Positives = 85/95 (89%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSE 542
+GQTREHALLAFTLGVKQ+ NKMD+T P YS+
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK 164
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 42.3 bits (95), Expect = 3e-04
Identities = 17/26 (65%), Positives = 22/26 (84%)
Frame = +1
Query: 547 RFEEIKKEVSSYIKKIGYNPAAVAFV 624
R++EI KEVSSY+KK+GYNP + FV
Sbjct: 166 RYDEIIKEVSSYLKKVGYNPDKIPFV 191
>At1g07940.1 68414.m00863 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 164 bits (398), Expect = 6e-41
Identities = 77/95 (81%), Positives = 85/95 (89%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSE 542
+GQTREHALLAFTLGVKQ+ NKMD+T P YS+
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK 164
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 42.3 bits (95), Expect = 3e-04
Identities = 17/26 (65%), Positives = 22/26 (84%)
Frame = +1
Query: 547 RFEEIKKEVSSYIKKIGYNPAAVAFV 624
R++EI KEVSSY+KK+GYNP + FV
Sbjct: 166 RYDEIIKEVSSYLKKVGYNPDKIPFV 191
>At1g07930.1 68414.m00862 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 164 bits (398), Expect = 6e-41
Identities = 77/95 (81%), Positives = 85/95 (89%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSE 542
+GQTREHALLAFTLGVKQ+ NKMD+T P YS+
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK 164
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 42.3 bits (95), Expect = 3e-04
Identities = 17/26 (65%), Positives = 22/26 (84%)
Frame = +1
Query: 547 RFEEIKKEVSSYIKKIGYNPAAVAFV 624
R++EI KEVSSY+KK+GYNP + FV
Sbjct: 166 RYDEIIKEVSSYLKKVGYNPDKIPFV 191
>At1g07920.1 68414.m00861 elongation factor 1-alpha / EF-1-alpha
identical to GB:CAA34456 from [Arabidopsis thaliana]
(Plant Mol. Biol. 14 (1), 107-110 (1990))
Length = 449
Score = 164 bits (398), Expect = 6e-41
Identities = 77/95 (81%), Positives = 85/95 (89%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITIDIALWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK
Sbjct: 70 GITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISK 129
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSE 542
+GQTREHALLAFTLGVKQ+ NKMD+T P YS+
Sbjct: 130 DGQTREHALLAFTLGVKQMICCCNKMDATTPKYSK 164
Score = 128 bits (310), Expect = 3e-30
Identities = 60/68 (88%), Positives = 62/68 (91%)
Frame = +1
Query: 52 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 231
MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVL
Sbjct: 1 MGKEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVL 60
Query: 232 DKLKAERD 255
DKLKAER+
Sbjct: 61 DKLKAERE 68
Score = 42.3 bits (95), Expect = 3e-04
Identities = 17/26 (65%), Positives = 22/26 (84%)
Frame = +1
Query: 547 RFEEIKKEVSSYIKKIGYNPAAVAFV 624
R++EI KEVSSY+KK+GYNP + FV
Sbjct: 166 RYDEIIKEVSSYLKKVGYNPDKIPFV 191
>At1g18070.1 68414.m02236 EF-1-alpha-related GTP-binding protein,
putative similar to EF-1-alpha-related GTP-binding
protein gi|1009232|gb|AAA79032
Length = 532
Score = 95.9 bits (228), Expect = 2e-20
Identities = 46/95 (48%), Positives = 61/95 (64%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
G T+++ FET TI+DAPGH+ ++ NMI+G SQAD VL+++A GEFE G +
Sbjct: 164 GKTVEVGRAHFETESTRFTILDAPGHKSYVPNMISGASQADIGVLVISARKGEFETGYER 223
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPPYSE 542
GQTREH LA TLGV +L NKMD +S+
Sbjct: 224 GGQTREHVQLAKTLGVSKLIVVVNKMDDPTVNWSK 258
Score = 68.1 bits (159), Expect = 5e-12
Identities = 28/64 (43%), Positives = 46/64 (71%)
Frame = +1
Query: 61 EKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKL 240
+K H+N+V IGHVD+GKST G +++ G +D R I+K+EKEA++ + S+ A+++D
Sbjct: 98 KKRHLNVVFIGHVDAGKSTIGGQILFLSGQVDDRQIQKYEKEAKDKSRESWYMAYIMDTN 157
Query: 241 KAER 252
+ ER
Sbjct: 158 EEER 161
>At5g10630.1 68418.m01231 elongation factor 1-alpha, putative /
EF-1-alpha, putative contains similarity to
SWISS-PROT:Q9YAV0 elongation factor 1-alpha (EF-1-alpha)
[Aeropyrum pernix]
Length = 667
Score = 90.6 bits (215), Expect = 8e-19
Identities = 41/88 (46%), Positives = 60/88 (68%), Gaps = 1/88 (1%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GIT+ +A+ F + +++V ++D+PGH+DF+ NMI G +QAD A+L++ A G FEAG
Sbjct: 302 GITMTVAVAYFNSKRHHVVLLDSPGHKDFVPNMIAGATQADAAILVIDASVGAFEAGFDN 361
Query: 438 -NGQTREHALLAFTLGVKQLHRRSNKMD 518
GQTREHA + GV+Q+ NKMD
Sbjct: 362 LKGQTREHARVLRGFGVEQVIVAINKMD 389
Score = 76.2 bits (179), Expect = 2e-14
Identities = 31/63 (49%), Positives = 46/63 (73%)
Frame = +1
Query: 67 THINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKA 246
+ +N+ ++GHVDSGKST +G L++ G I ++ + K+EKEA+ GKGSF YAW LD+
Sbjct: 238 SQLNLAIVGHVDSGKSTLSGRLLHLLGRISQKQMHKYEKEAKLQGKGSFAYAWALDESAE 297
Query: 247 ERD 255
ER+
Sbjct: 298 ERE 300
>At4g02930.1 68417.m00399 elongation factor Tu, putative / EF-Tu,
putative similar to mitochondrial elongation factor Tu
[Arabidopsis thaliana] gi|1149571|emb|CAA61511
Length = 454
Score = 69.3 bits (162), Expect = 2e-12
Identities = 38/92 (41%), Positives = 53/92 (57%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITI A ++ET+K + +D PGH D++KNMITG +Q D +L+V+ G
Sbjct: 115 GITIATAHVEYETAKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMP----- 169
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTEPP 533
QT+EH LLA +GV L NK+D + P
Sbjct: 170 --QTKEHILLARQVGVPSLVCFLNKVDVVDDP 199
Score = 32.7 bits (71), Expect = 0.23
Identities = 12/22 (54%), Positives = 16/22 (72%)
Frame = +1
Query: 58 KEKTHINIVVIGHVDSGKSTTT 123
+ K H+N+ IGHVD GK+T T
Sbjct: 63 RNKPHVNVGTIGHVDHGKTTLT 84
>At4g20360.1 68417.m02971 elongation factor Tu / EF-Tu (TUFA)
identical to SWISS-PROT:P17745 elongation factor Tu,
chloroplast precursor (EF-Tu) [Arabidopsis thaliana]
Length = 476
Score = 67.7 bits (158), Expect = 7e-12
Identities = 36/90 (40%), Positives = 51/90 (56%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITI+ A ++ET + +D PGH D++KNMITG +Q D A+L+V+ G
Sbjct: 127 GITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMP----- 181
Query: 438 NGQTREHALLAFTLGVKQLHRRSNKMDSTE 527
QT+EH LLA +GV + NK D +
Sbjct: 182 --QTKEHILLAKQVGVPDMVVFLNKEDQVD 209
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/45 (37%), Positives = 25/45 (55%)
Frame = +1
Query: 49 KMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEK 183
K ++K H+NI IGHVD GK+T T L I +K+++
Sbjct: 72 KFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDE 116
>At2g45030.1 68415.m05606 mitochondrial elongation factor, putative
similar to SP|P25039 Elongation factor G 1,
mitochondrial precursor (mEF-G-1) {Saccharomyces
cerevisiae}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03764: Elongation factor
G domain IV, PF00679: Elongation factor G C-terminus
Length = 754
Score = 35.5 bits (78), Expect = 0.033
Identities = 22/67 (32%), Positives = 30/67 (44%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 118 GITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITV 177
Query: 438 NGQTREH 458
+ Q R +
Sbjct: 178 DRQMRRY 184
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At1g45332.1 68414.m05195 mitochondrial elongation factor, putative
similar to mitochondrial elongation factor GI:3917 from
[Saccharomyces cerevisiae]
Length = 754
Score = 35.5 bits (78), Expect = 0.033
Identities = 22/67 (32%), Positives = 30/67 (44%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 437
GITI A Y V IID PGH DF + D A+L++ + G I+
Sbjct: 118 GITIQSAATYCTWKDYKVNIIDTPGHVDFTIEVERALRVLDGAILVLCSVGGVQSQSITV 177
Query: 438 NGQTREH 458
+ Q R +
Sbjct: 178 DRQMRRY 184
Score = 27.9 bits (59), Expect = 6.6
Identities = 11/26 (42%), Positives = 17/26 (65%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGI 153
NI + H+DSGK+T T +++ G I
Sbjct: 67 NIGISAHIDSGKTTLTERVLFYTGRI 92
>At4g18330.2 68417.m02719 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 471
Score = 34.7 bits (76), Expect = 0.058
Identities = 21/75 (28%), Positives = 35/75 (46%)
Frame = +3
Query: 294 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 473
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 474 TLGVKQLHRRSNKMD 518
+ +K + NK+D
Sbjct: 178 MMRLKHIIILQNKID 192
>At4g18330.1 68417.m02718 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to SP|Q09130 Eukaryotic translation
initiation factor 2 gamma subunit (eIF-2- gamma)
{Schizosaccharomyces pombe}; contains Pfam profile
PF00009: Elongation factor Tu GTP binding domain;
isoform predicted to contain a TG non-consensus acceptor
splice site.
Length = 284
Score = 34.7 bits (76), Expect = 0.058
Identities = 21/75 (28%), Positives = 35/75 (46%)
Frame = +3
Query: 294 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAF 473
T + +V+ +D PGH + M+ G + D A+L++AA QT EH
Sbjct: 124 TLRRHVSCVDCPGHDILMATMLNGAAIVDGALLLIAANES------CPQPQTAEHLASVD 177
Query: 474 TLGVKQLHRRSNKMD 518
+ +K + NK+D
Sbjct: 178 MMRLKHIIILQNKID 192
>At1g06220.2 68414.m00656 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.058
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +3
Query: 261 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g06220.1 68414.m00655 elongation factor Tu family protein
similar to Cryptosporidium parvum elongation factor-2
GB:U21667 GI:706974 from [Cryptosporidium parvum]
Length = 987
Score = 34.7 bits (76), Expect = 0.058
Identities = 18/51 (35%), Positives = 24/51 (47%)
Frame = +3
Query: 261 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+ + + L + Y I+D PGH +F M AD AVLIV A G
Sbjct: 195 VPMSLVLEDSRSKSYLCNIMDTPGHVNFSDEMTASLRLADGAVLIVDAAEG 245
>At1g04170.1 68414.m00407 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative similar to gb|U37354 from S. pombe. ESTs
gb|T41979, gb|N37284 and gb|N37529 come from this gene
Length = 465
Score = 34.3 bits (75), Expect = 0.076
Identities = 24/81 (29%), Positives = 37/81 (45%), Gaps = 4/81 (4%)
Frame = +3
Query: 288 FETSKY----YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTRE 455
FE SK +V+ +D PGH + M+ G + D A+L++AA QT E
Sbjct: 112 FENSKMKLLRHVSFVDCPGHDILMATMLNGAAIMDGALLLIAANE------TCPQPQTSE 165
Query: 456 HALLAFTLGVKQLHRRSNKMD 518
H + +K + NK+D
Sbjct: 166 HLAAVEIMQLKHIIILQNKID 186
>At2g18720.1 68415.m02180 eukaryotic translation initiation factor 2
subunit 3, putative / eIF2S3, putative / eIF-2-gamma,
putative
Length = 465
Score = 33.5 bits (73), Expect = 0.13
Identities = 21/71 (29%), Positives = 33/71 (46%)
Frame = +3
Query: 306 YVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGV 485
+V+ +D PGH + M+ G + D A+LI+AA QT EH + +
Sbjct: 120 HVSFVDCPGHDILMATMLNGAAIMDGALLIIAANE------TCPQPQTAEHLASVDMMHL 173
Query: 486 KQLHRRSNKMD 518
K + NK+D
Sbjct: 174 KDIIIIQNKID 184
>At5g39900.1 68418.m04839 GTP-binding protein LepA, putative
GTP-binding protein GUF1 - Saccharomyces cerevisiae,
PIR:S50374
Length = 661
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/41 (39%), Positives = 21/41 (51%)
Frame = +3
Query: 291 ETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
E S Y + +ID PGH DF + S A+L+V A G
Sbjct: 131 EASGYLLNLIDTPGHVDFSYEVSRSLSACQGALLVVDAAQG 171
Score = 27.9 bits (59), Expect = 6.6
Identities = 16/40 (40%), Positives = 19/40 (47%)
Frame = +1
Query: 40 D*PKMGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDK 159
D K EK N +I H+D GKST L+ G I K
Sbjct: 57 DLTKFPSEKIR-NFSIIAHIDHGKSTLADRLMELTGTIKK 95
>At3g22980.1 68416.m02898 elongation factor Tu family protein
similar to eukaryotic translation elongation factor 2
GB:NP_001952 [Homo sapiens]
Length = 1015
Score = 33.1 bits (72), Expect = 0.18
Identities = 16/51 (31%), Positives = 27/51 (52%)
Frame = +3
Query: 261 ITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
IT+ + + Y + +ID+PGH DF + T +D A+++V A G
Sbjct: 60 ITMKSSSISLKYKDYSLNLIDSPGHMDFCSEVSTAARLSDGALVLVDAVEG 110
Score = 32.7 bits (71), Expect = 0.23
Identities = 13/25 (52%), Positives = 16/25 (64%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGG 150
NI ++ HVD GK+T HLI GG
Sbjct: 11 NICILAHVDHGKTTLADHLIASSGG 35
>At4g11160.1 68417.m01808 translation initiation factor IF-2,
mitochondrial, putative similar to SP|P46198|IF2M_BOVIN
Translation initiation factor IF-2, mitochondrial
precursor (IF-2Mt) (IF-2(Mt)) {Bos taurus}
Length = 743
Score = 32.7 bits (71), Expect = 0.23
Identities = 14/35 (40%), Positives = 19/35 (54%)
Frame = +3
Query: 309 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+T +D PGH F + G + D VL+VAA G
Sbjct: 270 ITFLDTPGHAAFSEMRARGAAVTDIVVLVVAADDG 304
>At1g62750.1 68414.m07082 elongation factor Tu family protein
similar to elongation factor G SP:P34811 [Glycine max
(Soybean)]
Length = 783
Score = 32.7 bits (71), Expect = 0.23
Identities = 12/28 (42%), Positives = 20/28 (71%)
Frame = +1
Query: 64 KTHINIVVIGHVDSGKSTTTGHLIYKCG 147
K + NI ++ H+D+GK+TTT ++Y G
Sbjct: 94 KDYRNIGIMAHIDAGKTTTTERILYYTG 121
Score = 29.1 bits (62), Expect = 2.9
Identities = 17/52 (32%), Positives = 23/52 (44%)
Frame = +3
Query: 258 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
GITI A K+ + IID PGH DF + D A+ + + G
Sbjct: 146 GITITSAATTTFWDKHRINIIDTPGHVDFTLEVERALRVLDGAICLFDSVAG 197
>At5g08650.1 68418.m01029 GTP-binding protein LepA, putative
Length = 681
Score = 31.1 bits (67), Expect = 0.71
Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 4/56 (7%)
Frame = +3
Query: 258 GITIDIAL----WKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
GITI + + +E + + + +ID PGH DF + + + A+L+V A G
Sbjct: 133 GITIKLQAARMRYVYEDTPFCLNLIDTPGHVDFSYEVSRSLAACEGALLVVDASQG 188
Score = 28.3 bits (60), Expect = 5.0
Identities = 11/33 (33%), Positives = 18/33 (54%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEK 174
N +I H+D GKST L+ G + R +++
Sbjct: 88 NFSIIAHIDHGKSTLADKLLQVTGTVQNRDMKE 120
>At3g01360.1 68416.m00057 expressed protein contains Pfam profile
PF04819: Family of unknown function (DUF716) (Plant
viral-response family)
Length = 319
Score = 30.7 bits (66), Expect = 0.94
Identities = 21/71 (29%), Positives = 32/71 (45%), Gaps = 1/71 (1%)
Frame = +3
Query: 318 IDAPGHRDFIKNMITGTSQADCAV-LIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQL 494
I GH D+ + T Q +C + L+V TG F +KNG R+ LG +
Sbjct: 226 IKCKGHGDYHRAKAIATLQFNCHLALMVVVATGLFSVIANKNGYLRQDHSKYRPLGAELE 285
Query: 495 HRRSNKMDSTE 527
+ + +DS E
Sbjct: 286 NLSTFTLDSDE 296
>At1g76825.1 68414.m08940 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profile PF00009: Elongation
factor Tu GTP binding domain
Length = 630
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 315 IIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ID PGH F G+S D A+L+V
Sbjct: 113 VIDTPGHESFTNLRSRGSSLCDLAILVV 140
>At1g76810.1 68414.m08938 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to IF2
protein [Drosophila melanogaster] GI:7108770; contains
Pfam profile PF03144: Elongation factor Tu domain 2
Length = 1294
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 315 IIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ID PGH F G+S D A+L+V
Sbjct: 772 VIDTPGHESFTNLRSRGSSLCDLAILVV 799
>At1g76720.1 68414.m08929 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1201
Score = 29.9 bits (64), Expect = 1.6
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = +3
Query: 315 IIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ID PGH F G+S D A+L+V
Sbjct: 705 VIDTPGHESFTNLRSRGSSLCDLAILVV 732
>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
putative similar to ELONGATION FACTOR 2 GB:O14460 from
[Schizosaccharomyces pombe]
Length = 843
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/34 (32%), Positives = 20/34 (58%)
Frame = +3
Query: 297 SKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIV 398
++Y + +ID+PGH DF + D A+++V
Sbjct: 96 NEYLINLIDSPGHVDFSSEVTAALRITDGALVVV 129
Score = 29.1 bits (62), Expect = 2.9
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = +1
Query: 76 NIVVIGHVDSGKSTTTGHLIYKCG 147
N+ VI HVD GKST T L+ G
Sbjct: 21 NMSVIAHVDHGKSTLTDSLVAAAG 44
>At1g21160.1 68414.m02646 eukaryotic translation initiation factor 2
family protein / eIF-2 family protein similar to
SP|O60841 Translation initiation factor IF-2 {Homo
sapiens}; contains Pfam profiles PF00009: Elongation
factor Tu GTP binding domain, PF03144: Elongation factor
Tu domain 2
Length = 1088
Score = 29.5 bits (63), Expect = 2.2
Identities = 11/30 (36%), Positives = 17/30 (56%)
Frame = +3
Query: 309 VTIIDAPGHRDFIKNMITGTSQADCAVLIV 398
+ +ID PGH F G++ D A+L+V
Sbjct: 558 ILVIDTPGHESFTNLRSRGSNLCDLAILVV 587
>At4g24790.1 68417.m03550 expressed protein ; expression supported
by MPSS
Length = 815
Score = 28.7 bits (61), Expect = 3.8
Identities = 17/51 (33%), Positives = 28/51 (54%)
Frame = +1
Query: 406 VPVNSKLVSLRTVKPVSMPCSLSPSVSNSFIVGVTKWIPLNHHTVXPRFEE 558
VP+ LV+LR V+ + PC+ S S S + V KW +++ + +F E
Sbjct: 11 VPITRTLVALRRVRSLRDPCTTSMSKFASLLDNV-KWETGSNNGISLQFVE 60
>At1g78850.1 68414.m09191 curculin-like (mannose-binding) lectin
family protein low similarity to ser/thr protein kinase
from Zea mays [GI:2598067]; contains Pfam lectin
(probable mannose binding) domain PF01453
Length = 441
Score = 27.9 bits (59), Expect = 6.6
Identities = 19/68 (27%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
Frame = +1
Query: 124 GHLIYKCGGIDKRT--IEKFEKEAQEMGKGSFKYAWVLDKLKAERDLVSQSILLSGSSKL 297
G L+++ +K I+ E + S K+ W + LV QS+ L+G +KL
Sbjct: 120 GRLVWQTNTANKGAVGIKILENGNMVIYDSSGKFVWQSFDSPTDTLLVGQSLKLNGRTKL 179
Query: 298 ASTMLPSL 321
S + PS+
Sbjct: 180 VSRLSPSV 187
>At1g17220.1 68414.m02098 translation initiation factor IF-2,
chloroplast, putative similar to SP|P57997|IF2C_PHAVU
Translation initiation factor IF-2, chloroplast
precursor (PvIF2cp) {Phaseolus vulgaris}
Length = 1026
Score = 27.9 bits (59), Expect = 6.6
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 318 IDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 413
+D PGH F G D A+++VAA G
Sbjct: 557 LDTPGHEAFGAMRARGARVTDIAIIVVAADDG 588
>At3g03380.1 68416.m00336 DegP protease, putative contains
similarity to degP GI:2623992 from [Bradyrhizobium
japonicum]
Length = 1097
Score = 27.5 bits (58), Expect = 8.8
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +1
Query: 445 KPVSMPCSLSPSVSNSFIVGVTKWIPLNHHTVXPRFE 555
KP P S+SPS+ N+ +++ I L HH P E
Sbjct: 496 KPAIEPASVSPSIGNNGF-PISQDISLCHHDTEPMHE 531
>At2g28000.1 68415.m03393 RuBisCO subunit binding-protein alpha
subunit, chloroplast / 60 kDa chaperonin alpha subunit /
CPN-60 alpha identical to SWISS-PROT:P21238- RuBisCO
subunit binding-protein alpha subunit, chloroplast
precursor (60 kDa chaperonin alpha subunit, CPN-60
alpha) [Arabidopsis thaliana]
Length = 586
Score = 27.5 bits (58), Expect = 8.8
Identities = 17/58 (29%), Positives = 29/58 (50%)
Frame = -3
Query: 401 SYDEHSAISLRGSCDHVLDEISVSRSINDGNIVLASFELPESNIDCDTKSRSAFSLSN 228
++D+HS +L+ D + D + ++ N+VL F P+ D T +R A L N
Sbjct: 52 AFDQHSRAALQAGIDKLADCVGLTLGPRGRNVVLDEFGSPKVVNDGVTIAR-AIELPN 108
>At1g78860.1 68414.m09192 curculin-like (mannose-binding) lectin
family protein low similarity to Ser/Thr protein kinase
[Zea mays] GI:2598067; contains Pfam profile PF01453:
Lectin (probable mannose binding)
Length = 443
Score = 27.5 bits (58), Expect = 8.8
Identities = 13/36 (36%), Positives = 20/36 (55%)
Frame = +1
Query: 214 KYAWVLDKLKAERDLVSQSILLSGSSKLASTMLPSL 321
K+ W + LV QS+ L+G +KL S + PS+
Sbjct: 152 KFVWQSFDSPTDTLLVGQSLKLNGQNKLVSRLSPSV 187
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,486,425
Number of Sequences: 28952
Number of extensions: 328861
Number of successful extensions: 1081
Number of sequences better than 10.0: 33
Number of HSP's better than 10.0 without gapping: 1029
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1078
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1447936096
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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