BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060163.seq (563 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g19190.1 68415.m02239 light-responsive receptor protein kinas... 31 0.40 At5g11010.3 68418.m01286 pre-mRNA cleavage complex-related low s... 29 2.1 At5g11010.2 68418.m01285 pre-mRNA cleavage complex-related low s... 29 2.1 At4g01640.1 68417.m00213 hypothetical protein 28 3.7 At2g34280.1 68415.m04194 S locus F-box-related / SLF-related con... 28 3.7 At1g20570.1 68414.m02565 tubulin family protein 28 5.0 At5g22720.1 68418.m02654 F-box family protein contains F-box dom... 27 8.7 At3g45510.1 68416.m04914 zinc finger (C3HC4-type RING finger) fa... 27 8.7 >At2g19190.1 68415.m02239 light-responsive receptor protein kinase / senescence-responsive receptor-like serine/threonine kinase, putative (SIRK) similar to light repressible receptor protein kinase [Arabidopsis thaliana] gi|1321686|emb|CAA66376; contains Pfam profiles PF00069: Protein kinase domain and PF00560: Leucine Rich Repeat Length = 876 Score = 31.5 bits (68), Expect = 0.40 Identities = 23/91 (25%), Positives = 44/91 (48%), Gaps = 4/91 (4%) Frame = +3 Query: 219 QNDVQRVGVY---TITPAPDNVVKQXDPLYLQPHTVLITKEGVIQLIMKSKLPYAVEL-Q 386 ++DV +GV IT P + + +++ H I G I+ I+ +L ++ Sbjct: 754 KSDVYSLGVVLLEVITGQPAIASSKTEKVHISDHVRSILANGDIRGIVDQRLRERYDVGS 813 Query: 387 AWLLEEVIPQCCARASTRRPSRWTQIMV*LK 479 AW + E+ C S +RP+ +Q+++ LK Sbjct: 814 AWKMSEIALACTEHTSAQRPT-MSQVVMELK 843 >At5g11010.3 68418.m01286 pre-mRNA cleavage complex-related low similarity to SP|Q92989 Pre-mRNA cleavage complex II protein Clp1 {Homo sapiens} Length = 368 Score = 29.1 bits (62), Expect = 2.1 Identities = 15/36 (41%), Positives = 23/36 (63%), Gaps = 3/36 (8%) Frame = +3 Query: 225 DVQRVGVYTITPAPDNVVKQXDPL---YLQPHTVLI 323 D +R +Y ITP P+N+V++ D L Y+Q T L+ Sbjct: 315 DTERGILYVITPVPENLVEKVDLLLQGYIQLPTCLL 350 >At5g11010.2 68418.m01285 pre-mRNA cleavage complex-related low similarity to SP|Q92989 Pre-mRNA cleavage complex II protein Clp1 {Homo sapiens} Length = 368 Score = 29.1 bits (62), Expect = 2.1 Identities = 15/36 (41%), Positives = 23/36 (63%), Gaps = 3/36 (8%) Frame = +3 Query: 225 DVQRVGVYTITPAPDNVVKQXDPL---YLQPHTVLI 323 D +R +Y ITP P+N+V++ D L Y+Q T L+ Sbjct: 315 DTERGILYVITPVPENLVEKVDLLLQGYIQLPTCLL 350 >At4g01640.1 68417.m00213 hypothetical protein Length = 300 Score = 28.3 bits (60), Expect = 3.7 Identities = 18/45 (40%), Positives = 21/45 (46%) Frame = -1 Query: 377 HSVRELALHN*LDNTLLGDEHCVRLQVQWVTLLDHVVWSRGYGID 243 +S REL + N LD+ L E QV W DH W Y ID Sbjct: 185 YSSRELIMCN-LDDRLCVSEKMWPNQVIWSLDSDHKTWKEIYSID 228 >At2g34280.1 68415.m04194 S locus F-box-related / SLF-related contains Pfam PF00646: F-box domain; contains weak hit to TIGRFAM TIGR01640 : F-box protein interaction domain; weakly similar to self-incompatibility (S-) locus F-box (GI:29420811) [Prunus mume] Length = 391 Score = 28.3 bits (60), Expect = 3.7 Identities = 18/45 (40%), Positives = 21/45 (46%) Frame = -1 Query: 377 HSVRELALHN*LDNTLLGDEHCVRLQVQWVTLLDHVVWSRGYGID 243 +S REL + N LD+ L E QV W DH W Y ID Sbjct: 276 YSSRELIMCN-LDDRLCVSEKMWPNQVIWSLDSDHKTWKEIYSID 319 >At1g20570.1 68414.m02565 tubulin family protein Length = 976 Score = 27.9 bits (59), Expect = 5.0 Identities = 16/48 (33%), Positives = 24/48 (50%), Gaps = 3/48 (6%) Frame = +3 Query: 267 DNVVKQXDPLYLQPHTVLITKEG---VIQLIMKSKLPYAVELQAWLLE 401 D + K+ D + L L+ EG ++Q+ S LPY L +WL E Sbjct: 200 DYLYKRLDEVCLVQGGELVAVEGFHMLLQIFAGSLLPYVESLDSWLFE 247 >At5g22720.1 68418.m02654 F-box family protein contains F-box domain Pfam:PF00646 Length = 422 Score = 27.1 bits (57), Expect = 8.7 Identities = 16/54 (29%), Positives = 28/54 (51%), Gaps = 2/54 (3%) Frame = +1 Query: 64 IGEFKFGEDTFT-LRYVLEQGNLQVKFVAKDIASSLKYVNCKQAVIVNVDK-KY 219 + + K F LRY + L++ FV + + SSL++V K N+++ KY Sbjct: 308 VSDMKISSQAFAGLRYWTPKKELRLSFVPRCLLSSLEFVEIKGCSRSNMERVKY 361 >At3g45510.1 68416.m04914 zinc finger (C3HC4-type RING finger) family protein contains a zinc finger, C3HC4 type (RING finger), signature, PROSITE:PS00518 Length = 257 Score = 27.1 bits (57), Expect = 8.7 Identities = 14/75 (18%), Positives = 35/75 (46%), Gaps = 1/75 (1%) Frame = +3 Query: 324 TKEGVIQLIMKSKLPYAVELQ-AWLLEEVIPQCCARASTRRPSRWTQIMV*LKS*IKSWR 500 T +GV + K + P+ ++ +W + + + + W +++ +++ +++WR Sbjct: 171 TDDGVRRCCSKCRKPFCIDCNVSWHSNLSCKEYKMKGNKATTTVWLYVLLCMRNPMEAWR 230 Query: 501 LLAKSGRGQRKNYTF 545 LL+ S G F Sbjct: 231 LLSSSPIGDETGGRF 245 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 12,125,042 Number of Sequences: 28952 Number of extensions: 238457 Number of successful extensions: 522 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 519 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 522 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1082538160 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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