BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060157.seq (676 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_33597| Best HMM Match : No HMM Matches (HMM E-Value=.) 60 2e-09 SB_31539| Best HMM Match : No HMM Matches (HMM E-Value=.) 32 0.37 SB_42974| Best HMM Match : No HMM Matches (HMM E-Value=.) 30 1.5 SB_6679| Best HMM Match : Keratin_B2 (HMM E-Value=0.0084) 30 2.0 SB_34968| Best HMM Match : Plasmid_killer (HMM E-Value=4.3) 29 2.6 SB_58854| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 2.6 SB_25216| Best HMM Match : DUF1589 (HMM E-Value=8.4) 28 6.0 SB_3481| Best HMM Match : Viral_helicase1 (HMM E-Value=1.1) 28 6.0 SB_31773| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 7.9 >SB_33597| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 609 Score = 60.1 bits (139), Expect = 2e-09 Identities = 31/75 (41%), Positives = 44/75 (58%) Frame = +1 Query: 259 PWAMLHEFSEAVCRGCVNYEGADRIEVVLETARQLKRAHGFQEGRGPSHAKGAHRTPLEA 438 PWA+LH+FSE +CRGC NYEGA+RIE ++ +R L++ E R H G L + Sbjct: 19 PWAILHDFSEPICRGCCNYEGAERIEDIIHYSRLLRKNWEKSE-RNAQH--GVKNDRLNS 75 Query: 439 HQNGAETTRGQPAPP 483 + +T P+PP Sbjct: 76 TGSSVSST---PSPP 87 Score = 28.7 bits (61), Expect = 4.5 Identities = 9/11 (81%), Positives = 11/11 (100%) Frame = +2 Query: 221 KRQHCYLCDLP 253 +RQHC+LCDLP Sbjct: 6 QRQHCFLCDLP 16 >SB_31539| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 835 Score = 32.3 bits (70), Expect = 0.37 Identities = 27/109 (24%), Positives = 38/109 (34%) Frame = +1 Query: 118 FPKA**ISDNFQEKRRKPISRTPHYTRRRDVGDAETATLLPLRLAPMPWAMLHEFSEAVC 297 +PK + D + K T H R G T L L+ W + +SE + Sbjct: 723 WPKVLMVDDGTEFKGATTKLLTDHGVEARRAGPGTTGAKLSPSLSIAGWHSAYFYSEELA 782 Query: 298 RGCVNYEGADRIEVVLETARQLKRAHGFQEGRGPSHAKGAHRTPLEAHQ 444 G N E + +V+ K G P A R PLEA + Sbjct: 783 SGETNREWVTALPIVISDINATKTR---MTGVAPKDAIKLRRVPLEAEK 828 >SB_42974| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 477 Score = 30.3 bits (65), Expect = 1.5 Identities = 13/41 (31%), Positives = 22/41 (53%) Frame = +3 Query: 408 KRSTPYALRSPSKWSRNNKRTTCTSSSPYTDGWFTLHHPRP 530 + S + R+PS S + C ++S TDG+ T+H+ P Sbjct: 286 EHSPNFCERNPSAGSLGTQGRECNTTSMGTDGYLTMHYMTP 326 >SB_6679| Best HMM Match : Keratin_B2 (HMM E-Value=0.0084) Length = 292 Score = 29.9 bits (64), Expect = 2.0 Identities = 17/56 (30%), Positives = 25/56 (44%), Gaps = 3/56 (5%) Frame = +3 Query: 381 PRRSWSVACKRSTP---YALRSPSKWSRNNKRTTCTSSSPYTDGWFTLHHPRPTAS 539 PRRS+ V+C + +P Y + R + R +C SP + HP P S Sbjct: 150 PRRSYRVSCVQPSPRRSYRVSCVQPSPRRSYRVSCVQPSPRRSYRVSCVHPSPRRS 205 >SB_34968| Best HMM Match : Plasmid_killer (HMM E-Value=4.3) Length = 387 Score = 29.5 bits (63), Expect = 2.6 Identities = 19/75 (25%), Positives = 34/75 (45%), Gaps = 4/75 (5%) Frame = +1 Query: 157 KRRKPISRTPHYTRRRDVGDAETATLLPLRLAPMPWAML----HEFSEAVCRGCVNYEGA 324 +R + S+T H+ R GDA L L L P+ W + ++ S+ +C + Sbjct: 54 ERGRETSQTIHFNRGLPQGDALCTRLFTLCLNPVAWKLASTEGYKMSKPICAKITSLLYI 113 Query: 325 DRIEVVLETARQLKR 369 D ++V + +L R Sbjct: 114 DDLKVYAASEAKLNR 128 >SB_58854| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 222 Score = 29.5 bits (63), Expect = 2.6 Identities = 16/36 (44%), Positives = 19/36 (52%), Gaps = 3/36 (8%) Frame = +1 Query: 403 HAKGAHRTPLE-AHQNGAE--TTRGQPAPPAHHTPT 501 H + AHRT + H A TT+ QP PAH T T Sbjct: 35 HTEPAHRTSTQNQHTEPAHRTTTQNQPTEPAHRTTT 70 >SB_25216| Best HMM Match : DUF1589 (HMM E-Value=8.4) Length = 313 Score = 28.3 bits (60), Expect = 6.0 Identities = 16/54 (29%), Positives = 26/54 (48%) Frame = +1 Query: 352 ARQLKRAHGFQEGRGPSHAKGAHRTPLEAHQNGAETTRGQPAPPAHHTPTVGLR 513 AR +++A+ +E GP H +G R+ + T+ + HTP GLR Sbjct: 216 ARSIRQAYADREHEGPIHTQGLRRSRTRKSDPYSRLTQIENTQDPIHTP--GLR 267 >SB_3481| Best HMM Match : Viral_helicase1 (HMM E-Value=1.1) Length = 1347 Score = 28.3 bits (60), Expect = 6.0 Identities = 22/65 (33%), Positives = 26/65 (40%), Gaps = 2/65 (3%) Frame = +2 Query: 128 HNKSVTIFKRNEENRSLALRTTPGGE-MSVMQKRQH-CYLCDLPPCRGQCCMSFQKQCVE 301 H K I +RNE R L L GG M M + H C C + Q C F K Sbjct: 805 HEKRALIMRRNEVARYLTLSRAGGGRTMPAMLQDPHTCKRCS----QAQNCSVFHKSLES 860 Query: 302 GV*IT 316 G +T Sbjct: 861 GDHVT 865 >SB_31773| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 874 Score = 27.9 bits (59), Expect = 7.9 Identities = 13/27 (48%), Positives = 17/27 (62%), Gaps = 1/27 (3%) Frame = +2 Query: 80 CVFICDY-HGDSVFSRRHNKSVTIFKR 157 CV +C Y H SVF RR++ SV + R Sbjct: 689 CVVMCRYVHNVSVFMRRYDTSVRVVMR 715 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 20,705,728 Number of Sequences: 59808 Number of extensions: 429467 Number of successful extensions: 1235 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 1123 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 1223 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1733301648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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