Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060151.seq
(659 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 120 2e-28
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 118 6e-28
SPAC16A10.08c |mug74|SPAC589.01c|sequence orphan|Schizosaccharom... 27 1.8
SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces pom... 26 5.5
SPAC1420.01c ||SPAC56E4.08c|DUF1752 family protein|Schizosacchar... 25 7.3
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 25 9.7
SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22 |Schiz... 25 9.7
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 120 bits (288), Expect = 2e-28
Identities = 62/135 (45%), Positives = 82/135 (60%), Gaps = 2/135 (1%)
Frame = +2
Query: 254 LLSVAYKNVVGAXXXXXXXXXXXEQKTE--GSXXKQQMAKEYRVKVEKELREICYDVLGL 427
LLSVAYKNV+GA EQK E G+ + ++ KEYR K+E+EL IC D+L +
Sbjct: 46 LLSVAYKNVIGARRASWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTV 105
Query: 428 LDKHLIPKASNPESKVFYLQMKGDYYRTLQXVATXXTRHSVVEDSQKAYPDALXFSXAEM 607
L+KHLIP A++ ESKVFY +MKGDYYR L A R + S + Y A + AE+
Sbjct: 106 LEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAEL 165
Query: 608 QXTQPLKLGSCVKFS 652
T P++LG + FS
Sbjct: 166 APTHPIRLGLALNFS 180
Score = 46.4 bits (105), Expect = 4e-06
Identities = 23/43 (53%), Positives = 28/43 (65%)
Frame = +3
Query: 126 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERN 254
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERN
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERN 45
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 118 bits (285), Expect = 6e-28
Identities = 62/135 (45%), Positives = 79/135 (58%), Gaps = 2/135 (1%)
Frame = +2
Query: 254 LLSVAYKNVVGAXXXXXXXXXXXEQKTEGSXXKQQMA--KEYRVKVEKELREICYDVLGL 427
LLSVAYKN++GA EQK E +Q A KEYR K+E EL +IC+DVL +
Sbjct: 45 LLSVAYKNIIGARRASWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSV 104
Query: 428 LDKHLIPKASNPESKVFYLQMKGDYYRTLQXVATXXTRHSVVEDSQKAYPDALXFSXAEM 607
L+KHLIP A+ ESKVFY +MKGDYYR L + S +AY A + AE+
Sbjct: 105 LEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAEL 164
Query: 608 QXTQPLKLGSCVKFS 652
T P++LG + FS
Sbjct: 165 PPTDPMRLGLALNFS 179
Score = 45.2 bits (102), Expect = 8e-06
Identities = 24/44 (54%), Positives = 30/44 (68%)
Frame = +3
Query: 123 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERN 254
MS +E V AKLAEQAERY++M MK+V + +LS EERN
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERN 44
>SPAC16A10.08c |mug74|SPAC589.01c|sequence
orphan|Schizosaccharomyces pombe|chr 1|||Manual
Length = 285
Score = 27.5 bits (58), Expect = 1.8
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 5/62 (8%)
Frame = +1
Query: 16 FSAREKLILC-----LNLXVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNWPNKLS 180
FS +E ILC L+L V + ++ S+ + F +PR K N WPN+ S
Sbjct: 39 FSDKENSILCKQLKELDLVVSSNKEFLNEKTSDQISFLKPRETVVEKKLANGSIWPNETS 98
Query: 181 DM 186
+
Sbjct: 99 HL 100
>SPBC14F5.03c |kap123||karyopherin Kap123|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1067
Score = 25.8 bits (54), Expect = 5.5
Identities = 16/46 (34%), Positives = 21/46 (45%)
Frame = +1
Query: 28 EKLILCLNLXVQIHFSPSDKGISELVLFHRPRCPSTRKNWCNVPNW 165
EKL+ C HF +G+ + L RC +T CNVP W
Sbjct: 683 EKLVACTT-----HFY---EGVRKSALSSLWRCATTYYKVCNVPQW 720
>SPAC1420.01c ||SPAC56E4.08c|DUF1752 family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 580
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/42 (35%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +1
Query: 70 FSPSDK-GISELVLFHRPRCPSTRKNWCNVPNWPNKLSDMTT 192
FSP +K + +L LFH + PS+++ V N + SD +T
Sbjct: 162 FSPPEKPSMKDLALFHGNKSPSSKETIPKVSN--SNSSDTST 201
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 25.0 bits (52), Expect = 9.7
Identities = 14/52 (26%), Positives = 25/52 (48%)
Frame = +3
Query: 105 PLPSSTMSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNSF 260
P+ + T+S+ ++VQ AEQ E++ KE +E ++N F
Sbjct: 1092 PVYAETLSISSSKIVQSLSDAEQQEQHLAKVRMAKERQARIMEQFRMQQNKF 1143
>SPBC1861.09 |ppk22||serine/threonine protein kinase Ppk22
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 526
Score = 25.0 bits (52), Expect = 9.7
Identities = 12/38 (31%), Positives = 21/38 (55%)
Frame = +3
Query: 204 MKEVTETGVELSNEERNSFQLLIRMS*VPDGHXGVSFP 317
+ ++ E + S+++ +S L R+S PD H GV P
Sbjct: 64 VNQLNELDLNDSSDQLDSRPSLRRVSSAPDSHKGVEAP 101
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,416,526
Number of Sequences: 5004
Number of extensions: 41355
Number of successful extensions: 103
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 95
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 99
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -