BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060149.seq (680 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 167 2e-42 SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 162 4e-41 SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 152 5e-38 SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 136 2e-33 SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 135 5e-33 SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 78 1e-15 SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 33 0.038 SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual 27 2.5 SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyc... 26 4.4 SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 26 5.8 SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyc... 25 7.7 >SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces pombe|chr 3|||Manual Length = 647 Score = 167 bits (405), Expect = 2e-42 Identities = 78/102 (76%), Positives = 89/102 (87%) Frame = +3 Query: 255 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 434 DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ GE NVLIFDLGGGTFDVS+LT Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQGESNVLIFDLGGGTFDVSLLT 209 Query: 435 IEDGIFXVKSTAGDTHLGXEDFEQSMVYHFVQEFKXEYQKGL 560 IE+GIF VK+TAGDTHLG EDF+ +V HF+QEFK + +K + Sbjct: 210 IEEGIFEVKATAGDTHLGGEDFDSRLVNHFIQEFKRKNKKDI 251 Score = 130 bits (315), Expect = 1e-31 Identities = 59/82 (71%), Positives = 70/82 (85%) Frame = +1 Query: 7 EAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMK 186 +AKRLIGRKF+D VQ+DMKHWPF+V+S GKP ++V YKGE KTF PEE+SSMVL KM+ Sbjct: 67 DAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISSMVLMKMR 126 Query: 187 ETAEAYLGKTVQNAVITVPAYF 252 ETAEAYLG V +AV+TVPAYF Sbjct: 127 ETAEAYLGGKVTDAVVTVPAYF 148 >SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces pombe|chr 1|||Manual Length = 644 Score = 162 bits (394), Expect = 4e-41 Identities = 77/102 (75%), Positives = 87/102 (85%) Frame = +3 Query: 255 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 434 DSQRQATKDAG I+GLNVLRIINEPTAAAIAYGLD+ E NVLIFDLGGGTFDVS+LT Sbjct: 150 DSQRQATKDAGLIAGLNVLRIINEPTAAAIAYGLDRSNQHETNVLIFDLGGGTFDVSLLT 209 Query: 435 IEDGIFXVKSTAGDTHLGXEDFEQSMVYHFVQEFKXEYQKGL 560 IE+GIF VK+TAGDTHLG EDF+ +V HF QEFK + +K + Sbjct: 210 IEEGIFEVKATAGDTHLGGEDFDSRLVNHFAQEFKRKNKKDI 251 Score = 123 bits (296), Expect = 3e-29 Identities = 55/82 (67%), Positives = 68/82 (82%) Frame = +1 Query: 7 EAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMK 186 +AKRLIGR+F D VQ+DMKHWPF+V+ GKP I+V +KGE KTF PEE+SSMVL KM+ Sbjct: 67 DAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISSMVLLKMR 126 Query: 187 ETAEAYLGKTVQNAVITVPAYF 252 E+AEA+LG V +AV+TVPAYF Sbjct: 127 ESAEAFLGGKVTDAVVTVPAYF 148 >SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces pombe|chr 2|||Manual Length = 613 Score = 152 bits (368), Expect = 5e-38 Identities = 71/95 (74%), Positives = 81/95 (85%) Frame = +3 Query: 255 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 434 DSQR ATKDAG I+GLNVLRIINEPTAAAIAYGLD K +NVLIFDLGGGTFDVS+L Sbjct: 154 DSQRAATKDAGAIAGLNVLRIINEPTAAAIAYGLDAKSDKPKNVLIFDLGGGTFDVSLLK 213 Query: 435 IEDGIFXVKSTAGDTHLGXEDFEQSMVYHFVQEFK 539 I+ G+F V +TAGDTHLG EDF+ ++V HF+QEFK Sbjct: 214 IQGGVFEVLATAGDTHLGGEDFDNALVEHFIQEFK 248 Score = 109 bits (263), Expect = 3e-25 Identities = 50/82 (60%), Positives = 63/82 (76%) Frame = +1 Query: 7 EAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMK 186 +AKRLIGR++ED Q D+KHWPF+V+ + G P I+V Y GE K F +E+S+MVLTKMK Sbjct: 71 DAKRLIGRRYEDPETQKDIKHWPFKVIDNNGIPTIEVNYLGEKKQFTAQEISAMVLTKMK 130 Query: 187 ETAEAYLGKTVQNAVITVPAYF 252 E +EA L K V+ AVITVPAYF Sbjct: 131 EISEAKLNKRVEKAVITVPAYF 152 >SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||Manual Length = 663 Score = 136 bits (330), Expect = 2e-33 Identities = 65/126 (51%), Positives = 95/126 (75%) Frame = +3 Query: 255 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 434 D+QRQATKDAGTI+GLNV+RI+NEPTAAAIAYGLDK T E++++++DLGGGTFDVS+L+ Sbjct: 183 DAQRQATKDAGTIAGLNVIRIVNEPTAAAIAYGLDKTDT-EKHIVVYDLGGGTFDVSLLS 241 Query: 435 IEDGIFXVKSTAGDTHLGXEDFEQSMVYHFVQEFKXEYQKGLRYQQESSLGVFVLXVKRA 614 I++G+F V +T+GDTHLG EDF+ ++ + + + + + + ++G V++A Sbjct: 242 IDNGVFEVLATSGDTHLGGEDFDNRVINYLARTYNRKNNVDVT-KDLKAMGKLKREVEKA 300 Query: 615 KXXLLS 632 K L S Sbjct: 301 KRTLSS 306 Score = 105 bits (252), Expect = 6e-24 Identities = 47/91 (51%), Positives = 68/91 (74%) Frame = +1 Query: 7 EAKRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMK 186 + KRLIGRKF++ T+ D+K +PF +V+D +P ++V G+ K F PEE+S+M+L+KMK Sbjct: 100 DIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSKMK 159 Query: 187 ETAEAYLGKTVQNAVITVPAYFKTLKDKPQK 279 +TAEAYLGK V +AV+TVPAYF + + K Sbjct: 160 QTAEAYLGKPVTHAVVTVPAYFNDAQRQATK 190 >SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces pombe|chr 1|||Manual Length = 674 Score = 135 bits (327), Expect = 5e-33 Identities = 63/95 (66%), Positives = 78/95 (82%) Frame = +3 Query: 255 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGERNVLIFDLGGGTFDVSILT 434 DSQRQATK AG I+GLNVLR++NEPTAAA+AYGLDKK + V +FDLGGGTFD+SIL Sbjct: 196 DSQRQATKAAGAIAGLNVLRVVNEPTAAALAYGLDKKN--DAIVAVFDLGGGTFDISILE 253 Query: 435 IEDGIFXVKSTAGDTHLGXEDFEQSMVYHFVQEFK 539 + +G+F V+ST GDTHLG EDF+ ++V H V+ FK Sbjct: 254 LNNGVFEVRSTNGDTHLGGEDFDVALVRHIVETFK 288 Score = 77.8 bits (183), Expect = 1e-15 Identities = 36/80 (45%), Positives = 52/80 (65%) Frame = +1 Query: 13 KRLIGRKFEDATVQADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKET 192 KRLIGR+F++ VQ D+K P+++V + KT+ P ++ +L+KM+ET Sbjct: 118 KRLIGRRFKEPEVQRDIKEVPYKIVEHSNGD---AWLEARGKTYSPSQIGGFILSKMRET 174 Query: 193 AEAYLGKTVQNAVITVPAYF 252 A YLGK V+NAV+TVPAYF Sbjct: 175 ASTYLGKDVKNAVVTVPAYF 194 >SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Schizosaccharomyces pombe|chr 1|||Manual Length = 720 Score = 78.2 bits (184), Expect = 1e-15 Identities = 40/103 (38%), Positives = 61/103 (59%), Gaps = 4/103 (3%) Frame = +3 Query: 255 DSQRQATKDAGTISGLNVLRIINEPTAAAIAYGLDKKGTGE----RNVLIFDLGGGTFDV 422 D QR+A +A I+GLN LR++N+ AAA+ YG+ K E R V I D G + V Sbjct: 154 DIQRRALLEAANIAGLNPLRLMNDNAAAALTYGITKTDLPEPESPRRVAIVDFGHSNYSV 213 Query: 423 SILTIEDGIFXVKSTAGDTHLGXEDFEQSMVYHFVQEFKXEYQ 551 SI+ G F +KST D +LG + +++++ +F EFK +Y+ Sbjct: 214 SIVEFSRGQFHIKSTVCDRNLGSRNMDKALIDYFAAEFKEKYK 256 Score = 41.5 bits (93), Expect = 1e-04 Identities = 25/85 (29%), Positives = 44/85 (51%), Gaps = 1/85 (1%) Frame = +1 Query: 13 KRLIGRKFEDATVQ-ADMKHWPFEVVSDGGKPKIKVAYKGEDKTFFPEEVSSMVLTKMKE 189 KRL GR ++D ++ + ++ G KV Y E+ F ++ + TK+K Sbjct: 72 KRLAGRTYDDPEIKDIESNFISAKLTEVDGFVGAKVQYLNEETAFSNIQLIAAYFTKIKA 131 Query: 190 TAEAYLGKTVQNAVITVPAYFKTLK 264 AEA L +V + VI++PA+F ++ Sbjct: 132 IAEAELIGSVSDVVISIPAWFTDIQ 156 >SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pombe|chr 1|||Manual Length = 566 Score = 33.1 bits (72), Expect = 0.038 Identities = 16/47 (34%), Positives = 25/47 (53%) Frame = +1 Query: 130 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFKTLKDK 270 ++K E S L ++ E+AE +LG V V++VP YF + K Sbjct: 134 KEKILTAHEASVRHLRRLTESAEDFLGTKVNGCVMSVPVYFTDAQRK 180 >SPAPB1E7.04c |||chitinase |Schizosaccharomyces pombe|chr 1|||Manual Length = 1236 Score = 27.1 bits (57), Expect = 2.5 Identities = 28/126 (22%), Positives = 52/126 (41%) Frame = -1 Query: 410 TAAEVKDKYISFSSTLFVKTVSNRSSSGFIDDSENVQA*DGTCIFCGLSLRVLKYAGTVI 231 +++ + + S SS L T S S+S I S + F S + I Sbjct: 565 SSSSIPSTFSSVSSILSSSTSSPSSTSLSISSSST------SSTFSSASTSSPSSISSSI 618 Query: 230 TAFCTVLPR*ASAVSFIFVSTMELTSSGKKVLSSPLYATLILGLPPSLTTSKGQCFMSAC 51 ++ T+L + S + +S+ + S +LSS + + + SL+T S+ Sbjct: 619 SSSSTILSSPTPSTSSLMISSSSIISGSSSILSSSIST---IPISSSLSTYSSSVIPSSS 675 Query: 50 TVASSN 33 T+ SS+ Sbjct: 676 TLVSSS 681 >SPCC70.05c |||serine/threonine protein kinase |Schizosaccharomyces pombe|chr 3|||Manual Length = 781 Score = 26.2 bits (55), Expect = 4.4 Identities = 12/48 (25%), Positives = 23/48 (47%) Frame = -2 Query: 508 DCSKSSXPKWVSPAVDFTSKIPSSMVRMDTSKVPPPRSKISTFRSPVP 365 D S+ + V+ D + +P + + T + PPR K + +P+P Sbjct: 61 DYKSSAHEQRVNHTTDSMAHVPGNNSPLQTPQKSPPRQKHTAPATPIP 108 >SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 848 Score = 25.8 bits (54), Expect = 5.8 Identities = 11/45 (24%), Positives = 24/45 (53%) Frame = +1 Query: 130 EDKTFFPEEVSSMVLTKMKETAEAYLGKTVQNAVITVPAYFKTLK 264 +++ + EE+ +M + AE + + + V+TVP +F L+ Sbjct: 131 DEENYSLEEIIAMTMEHYISLAEEMAHEKITDLVLTVPPHFNELQ 175 >SPBC2D10.17 |clr1||cryptic loci regulator Clr1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1238 Score = 25.4 bits (53), Expect = 7.7 Identities = 15/37 (40%), Positives = 20/37 (54%) Frame = -2 Query: 502 SKSSXPKWVSPAVDFTSKIPSSMVRMDTSKVPPPRSK 392 +KSS K VSP V SK +S V + S +P +K Sbjct: 203 NKSSPVKHVSPNVKNNSKKTASSVNSNHSSIPSSITK 239 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,702,915 Number of Sequences: 5004 Number of extensions: 54323 Number of successful extensions: 203 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 180 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 199 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 313902888 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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