Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060147.seq
(693 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 157 9e-41
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 157 9e-41
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 149 2e-38
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 149 3e-38
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.8
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.6
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 157 bits (381), Expect = 9e-41
Identities = 80/121 (66%), Positives = 85/121 (70%), Gaps = 1/121 (0%)
Frame = +2
Query: 317 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 496
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72
Query: 497 NGQTVXXXXXXXXXXXXXXXXE*TKMDXPEPPYSEPDLRKSRGSI-PYIQXIGYXPSCCR 673
NGQT KMD EPPYSE + + + YI+ IGY P+
Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 132
Query: 674 F 676
F
Sbjct: 133 F 133
Score = 29.9 bits (64), Expect = 0.024
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +3
Query: 618 QEEVSPTSXRLATXPAAVAFVPISG 692
++EVS ++ PAAVAFVPISG
Sbjct: 114 KKEVSSYIKKIGYNPAAVAFVPISG 138
Score = 27.5 bits (58), Expect = 0.13
Identities = 11/11 (100%), Positives = 11/11 (100%)
Frame = +3
Query: 282 WVLDKLKAERE 314
WVLDKLKAERE
Sbjct: 1 WVLDKLKAERE 11
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 157 bits (381), Expect = 9e-41
Identities = 80/121 (66%), Positives = 85/121 (70%), Gaps = 1/121 (0%)
Frame = +2
Query: 317 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 496
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129
Query: 497 NGQTVXXXXXXXXXXXXXXXXE*TKMDXPEPPYSEPDLRKSRGSI-PYIQXIGYXPSCCR 673
NGQT KMD EPPYSE + + + YI+ IGY P+
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 189
Query: 674 F 676
F
Sbjct: 190 F 190
Score = 103 bits (247), Expect = 2e-24
Identities = 48/51 (94%), Positives = 48/51 (94%)
Frame = +1
Query: 112 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEWVK 264
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQE K
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51
Score = 46.8 bits (106), Expect = 2e-07
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +3
Query: 255 MGKGSFKYAWVLDKLKAERE 314
MGKGSFKYAWVLDKLKAERE
Sbjct: 49 MGKGSFKYAWVLDKLKAERE 68
Score = 29.9 bits (64), Expect = 0.024
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +3
Query: 618 QEEVSPTSXRLATXPAAVAFVPISG 692
++EVS ++ PAAVAFVPISG
Sbjct: 171 KKEVSSYIKKIGYNPAAVAFVPISG 195
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 149 bits (362), Expect = 2e-38
Identities = 76/117 (64%), Positives = 81/117 (69%), Gaps = 1/117 (0%)
Frame = +2
Query: 329 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 508
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 509 VXXXXXXXXXXXXXXXXE*TKMDXPEPPYSEPDLRKSRGSI-PYIQXIGYXPSCCRF 676
KMD EPPYSE + + + YI+ IGY P+ F
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAF 117
Score = 29.9 bits (64), Expect = 0.024
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +3
Query: 618 QEEVSPTSXRLATXPAAVAFVPISG 692
++EVS ++ PAAVAFVPISG
Sbjct: 98 KKEVSSYIKKIGYNPAAVAFVPISG 122
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 149 bits (360), Expect = 3e-38
Identities = 76/121 (62%), Positives = 83/121 (68%), Gaps = 1/121 (0%)
Frame = +2
Query: 317 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 496
GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISK
Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129
Query: 497 NGQTVXXXXXXXXXXXXXXXXE*TKMDXPEPPYSEPDLRKSRGSI-PYIQXIGYXPSCCR 673
NGQT KMD +PPYSE + + + YI+ IGY +
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVA 189
Query: 674 F 676
F
Sbjct: 190 F 190
Score = 103 bits (247), Expect = 2e-24
Identities = 48/51 (94%), Positives = 48/51 (94%)
Frame = +1
Query: 112 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEWVK 264
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQE K
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGK 51
Score = 46.8 bits (106), Expect = 2e-07
Identities = 20/20 (100%), Positives = 20/20 (100%)
Frame = +3
Query: 255 MGKGSFKYAWVLDKLKAERE 314
MGKGSFKYAWVLDKLKAERE
Sbjct: 49 MGKGSFKYAWVLDKLKAERE 68
Score = 25.4 bits (53), Expect = 0.52
Identities = 12/25 (48%), Positives = 17/25 (68%)
Frame = +3
Query: 618 QEEVSPTSXRLATXPAAVAFVPISG 692
++EVS ++ A+VAFVPISG
Sbjct: 171 KKEVSSYIKKIGYNTASVAFVPISG 195
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +2
Query: 368 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 472
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.52
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +1
Query: 124 KTHINIVVIGHVDSGKST 177
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +1
Query: 133 INIVVIGHVDSGKST 177
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 353 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 454
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.6
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 353 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 454
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 185,346
Number of Sequences: 438
Number of extensions: 3618
Number of successful extensions: 28
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 20
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21195810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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