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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060146.seq
         (511 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At2g24450.1 68415.m02922 fasciclin-like arabinogalactan family p...    28   4.2  
At1g47340.1 68414.m05241 F-box family protein contains F-box dom...    28   4.2  
At5g60760.1 68418.m07623 2-phosphoglycerate kinase-related conta...    27   5.5  
At5g53870.1 68418.m06701 plastocyanin-like domain-containing pro...    27   5.5  
At5g17770.1 68418.m02084 NADH-cytochrome b5 reductase identical ...    27   5.5  
At4g36650.1 68417.m05201 transcription factor IIB (TFIIB) family...    27   7.3  
At4g10670.1 68417.m01743 transcription elongation factor-related...    27   7.3  
At5g61460.1 68418.m07712 structural maintenance of chromosomes (...    27   9.7  
At5g18760.1 68418.m02228 zinc finger (C3HC4-type RING finger) fa...    27   9.7  
At4g10710.1 68417.m01751 transcriptional regulator-related simil...    27   9.7  
At2g39300.1 68415.m04825 expressed protein ; expression supporte...    27   9.7  

>At2g24450.1 68415.m02922 fasciclin-like arabinogalactan family
           protein similar to fasciclin-like
           arabinogalactan-protein 1 [Arabidopsis thaliana]
           gi|13377776|gb|AAK20857
          Length = 280

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 17/51 (33%), Positives = 22/51 (43%)
 Frame = +2

Query: 248 GLESKAQLPTKRRRCSPFAASPSTSPGVKTSESKPSSFGESVSAPXXITPE 400
           GL S  ++P      SP A SP    G  T    P+  G+   AP  + PE
Sbjct: 173 GLGSPVKVPPPPPMSSPPAPSPKK--GAATPAPAPADEGDYADAPPGLAPE 221


>At1g47340.1 68414.m05241 F-box family protein contains F-box domain
           Pfam:PF00646
          Length = 459

 Score = 27.9 bits (59), Expect = 4.2
 Identities = 23/64 (35%), Positives = 28/64 (43%)
 Frame = +2

Query: 251 LESKAQLPTKRRRCSPFAASPSTSPGVKTSESKPSSFGESVSAPXXITPERLAQETYDEI 430
           L+  A    KRRRCS FAA       V    S   +F  SVS P  +  E L +     +
Sbjct: 2   LKKTAPNSPKRRRCSSFAA-------VDLVGSANRTFMVSVSLPKELILEILKRLPAKSV 54

Query: 431 KRLH 442
           KR H
Sbjct: 55  KRFH 58


>At5g60760.1 68418.m07623 2-phosphoglycerate kinase-related contains
           weak similarity to 2-phosphoglycerate kinase (GI:467751)
           [Methanothermus fervidus]
          Length = 738

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 16/73 (21%), Positives = 33/73 (45%)
 Frame = +2

Query: 203 WA*KENGXCYIKKKSGLESKAQLPTKRRRCSPFAASPSTSPGVKTSESKPSSFGESVSAP 382
           WA   +   Y+   +  ESKA+    ++  S        S G K   ++ +  G + + P
Sbjct: 224 WASTYHAGEYLDPVAVAESKARRRRAKKMDSIEDEKAKASEGGKAKNTQQTDVGSTKNTP 283

Query: 383 XXITPERLAQETY 421
             ++P+++A E +
Sbjct: 284 VLLSPKQMAVEGF 296


>At5g53870.1 68418.m06701 plastocyanin-like domain-containing
           protein contains similarity to SP|Q02917 Early nodulin
           55-2 precursor {Glycine max}; PF02298: Plastocyanin-like
           domain
          Length = 370

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 14/33 (42%), Positives = 18/33 (54%)
 Frame = +2

Query: 302 AASPSTSPGVKTSESKPSSFGESVSAPXXITPE 400
           A SPS SP    S S  SS  +S + P  +TP+
Sbjct: 266 AHSPSHSPATPKSPSPSSSPAQSPATPSPMTPQ 298


>At5g17770.1 68418.m02084 NADH-cytochrome b5 reductase identical to
           NADH-cytochrome b5 reductase [Arabidopsis thaliana]
           GI:4240116
          Length = 281

 Score = 27.5 bits (58), Expect = 5.5
 Identities = 21/58 (36%), Positives = 31/58 (53%), Gaps = 2/58 (3%)
 Frame = -1

Query: 235 NVAXAIFFLRPTKSS--GLRICQRICFNXEYKKMKNNIKPHTGNTRKKYXIRFQVTVK 68
           NVA  +F L PT +S  GL I Q I    +  + ++ IKP+T  T      RF++ +K
Sbjct: 61  NVAKFVFEL-PTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIK 117


>At4g36650.1 68417.m05201 transcription factor IIB (TFIIB) family
           protein contains Pfam domain, PF00382: Transcription
           factor TFIIB repeat
          Length = 503

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 22/68 (32%), Positives = 32/68 (47%), Gaps = 2/68 (2%)
 Frame = +2

Query: 236 KKKSGLESKAQLPTKRRRCSPFAASPSTSPGVKTS--ESKPSSFGESVSAPXXITPERLA 409
           +++  L  K  LP   R  +PF ++PS S G+ T     +PS    S S    I P +L 
Sbjct: 423 QQQQQLGDKETLPIYLRDHNPFPSNPSPSTGISTINWSFRPSVVPGSSSNLPVIHPPKL- 481

Query: 410 QETYDEIK 433
              Y EI+
Sbjct: 482 PPGYAEIR 489


>At4g10670.1 68417.m01743 transcription elongation factor-related
           low similarity to chromatin-specific transcription
           elongation factor FACT 140 kDa subunit [Homo sapiens]
           GI:5499741
          Length = 470

 Score = 27.1 bits (57), Expect = 7.3
 Identities = 16/52 (30%), Positives = 23/52 (44%)
 Frame = +2

Query: 191 RGFRWA*KENGXCYIKKKSGLESKAQLPTKRRRCSPFAASPSTSPGVKTSES 346
           +G  WA  E       ++ G+ES ++   KRR+   F  S   S G   S S
Sbjct: 406 KGKTWAELEREATNADREHGVESDSEEERKRRKMKAFGKSRPGSSGAGGSSS 457


>At5g61460.1 68418.m07712 structural maintenance of chromosomes
           (SMC) family protein very strong similarity to SMC-like
           protein (MIM) [Arabidopsis thaliana] GI:5880614;
           contains Pfam profile PF02463: RecF/RecN/SMC N terminal
           domain
          Length = 1057

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 16/41 (39%), Positives = 21/41 (51%)
 Frame = +2

Query: 332 KTSESKPSSFGESVSAPXXITPERLAQETYDEIKRLHRRGQ 454
           K SE  P S  ES+      TPE+L+ +     +RLHR  Q
Sbjct: 827 KASEICPESEIESLGPWDGSTPEQLSAQITRMNQRLHRENQ 867


>At5g18760.1 68418.m02228 zinc finger (C3HC4-type RING finger)
           family protein predicted proteins, Arabidopsis thaliana
           ; contains Pfam profile PF00097: Zinc finger, C3HC4 type
           (RING finger)
          Length = 411

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 23/66 (34%), Positives = 26/66 (39%), Gaps = 11/66 (16%)
 Frame = +2

Query: 233 IKKKSGLESKAQLPTKRRRC-----------SPFAASPSTSPGVKTSESKPSSFGESVSA 379
           I +   L+ KA LP   RR             P  ASP TSP  K   S PSS    +S 
Sbjct: 66  ILRNLSLKRKASLPNYERRLLLSPSVSETSEKPLVASPITSPYWKRCLSLPSSNSAKLSL 125

Query: 380 PXXITP 397
               TP
Sbjct: 126 VVSTTP 131


>At4g10710.1 68417.m01751 transcriptional regulator-related similar to
            chromatin-specific transcription elongation factor FACT
            140 kDa subunit (GI:5499741) [Homo sapiens]
          Length = 1074

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
 Frame = +2

Query: 191  RGFRWA*KENGXCYIKKKSGLESKAQLPTKRRRCSPFAAS-PSTSPGVKTSESK 349
            +G  W   E       ++ G+ES ++   KRR+   F  S P TS G  +S  K
Sbjct: 1011 KGKTWDELEREATNADREHGVESDSEEERKRRKMKAFGKSRPGTSGGGGSSSMK 1064


>At2g39300.1 68415.m04825 expressed protein ; expression supported
           by MPSS
          Length = 768

 Score = 26.6 bits (56), Expect = 9.7
 Identities = 24/71 (33%), Positives = 32/71 (45%), Gaps = 3/71 (4%)
 Frame = +2

Query: 236 KKKSGLESKAQLPTKRRRCSPFAASPSTSPGVKTSESKPSSFG---ESVSAPXXITPERL 406
           K+KSG    + L   RRR  P A SP  SP  ++ + K  S G    S  +      ERL
Sbjct: 120 KQKSGSLHSSSLSGSRRRLPPRAQSP--SPLSESGKDKRKSKGLRDASARSLARSVIERL 177

Query: 407 AQETYDEIKRL 439
           +  T  + K L
Sbjct: 178 SHNTQGKSKAL 188


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,629,735
Number of Sequences: 28952
Number of extensions: 197593
Number of successful extensions: 511
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 499
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 510
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 917929344
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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