BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060144.seq (671 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosa... 27 2.5 SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase... 25 9.9 SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1||... 25 9.9 >SPAC1B9.02c |sck1||serine/threonine protein kinase Sck1|Schizosaccharomyces pombe|chr 1|||Manual Length = 696 Score = 27.1 bits (57), Expect = 2.5 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = +2 Query: 512 QKELRMLANTAPQFISNFITAVTELYVHDLSTKKMPPKNLL 634 Q E R A +I+ + A+ L+ HD+ + + P+N+L Sbjct: 395 QHEGRFPEQRAKFYIAELVLALEHLHKHDIIYRDLKPENIL 435 >SPACUNK12.02c |cmk1||calcium/calmodulin-dependent protein kinase Cmk1|Schizosaccharomyces pombe|chr 1|||Manual Length = 335 Score = 25.0 bits (52), Expect = 9.9 Identities = 16/45 (35%), Positives = 22/45 (48%) Frame = -2 Query: 385 DTAMLARILSTFNLL*LSIPPNSDKNIVFFVVSLLQEDKYSHKIL 251 D ++ R L NLL S PNSD I F +S ED + ++ Sbjct: 147 DNGIVHRDLKPENLLYRSKDPNSDLLIADFGLSHFYEDSQYYMLM 191 >SPAC24H6.09 |gef1||RhoGEF Gef1|Schizosaccharomyces pombe|chr 1|||Manual Length = 753 Score = 25.0 bits (52), Expect = 9.9 Identities = 18/59 (30%), Positives = 30/59 (50%), Gaps = 2/59 (3%) Frame = +3 Query: 243 TTHRI--LCEYLSSCNNETTKNTIFLSLFGGMESQRRLKVLSILASMAVSASSTPVLLA 413 T+ R+ L +++ ++ET+ N L G ++ + LASM S SST LL+ Sbjct: 188 TSRRVSGLGSFMAQNSSETSSNRTSAYLPGSSTDEQEKRSSVTLASMPSSHSSTASLLS 246 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,718,881 Number of Sequences: 5004 Number of extensions: 52934 Number of successful extensions: 98 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 98 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 98 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 307866294 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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