BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060140.seq (686 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 35 0.010 SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual 33 0.029 SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosacch... 30 0.36 SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyce... 27 2.5 SPAC652.01 |||BC10 family protein|Schizosaccharomyces pombe|chr ... 25 7.8 >SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF hand and WH2 motif |Schizosaccharomyces pombe|chr 1|||Manual Length = 1794 Score = 35.1 bits (77), Expect = 0.010 Identities = 16/28 (57%), Positives = 16/28 (57%) Frame = +1 Query: 415 QPTVFKQSQPTGFKQSQPTVFKQSQTFG 498 QPT F Q QPTGF QP F Q Q G Sbjct: 126 QPTGFVQPQPTGFMSQQPASFMQPQRTG 153 Score = 28.3 bits (60), Expect = 1.1 Identities = 13/26 (50%), Positives = 13/26 (50%) Frame = +1 Query: 394 PAVFKQSQPTVFKQSQPTGFKQSQPT 471 P F Q QPT F QP F Q Q T Sbjct: 127 PTGFVQPQPTGFMSQQPASFMQPQRT 152 Score = 27.1 bits (57), Expect = 2.5 Identities = 14/27 (51%), Positives = 16/27 (59%), Gaps = 1/27 (3%) Frame = +1 Query: 394 PAVFKQSQPT-VFKQSQPTGFKQSQPT 471 P F Q Q T V QPTG+ Q+QPT Sbjct: 208 PTGFVQPQQTGVVMPPQPTGYLQAQPT 234 >SPCC162.07 |ent1||epsin|Schizosaccharomyces pombe|chr 3|||Manual Length = 706 Score = 33.5 bits (73), Expect = 0.029 Identities = 17/30 (56%), Positives = 18/30 (60%) Frame = +1 Query: 409 QSQPTVFKQSQPTGFKQSQPTVFKQSQTFG 498 Q QPT F Q QPTGF + Q T F Q Q G Sbjct: 310 QPQPTGFLQQQPTGFIRPQNTGFVQPQYTG 339 Score = 33.1 bits (72), Expect = 0.039 Identities = 18/39 (46%), Positives = 20/39 (51%) Frame = +1 Query: 382 ISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQTFG 498 + P F Q QPT F + Q TGF Q Q T F Q Q G Sbjct: 309 VQPQPTGFLQQQPTGFIRPQNTGFVQPQYTGFVQPQHTG 347 Score = 28.7 bits (61), Expect = 0.83 Identities = 15/35 (42%), Positives = 17/35 (48%) Frame = +1 Query: 403 FKQSQPTVFKQSQPTGFKQSQPTVFKQSQTFGRQE 507 F Q Q T F Q Q GF QPT + Q Q G + Sbjct: 436 FIQPQRTGFVQPQQNGFFNPQPTGYMQPQRTGMMQ 470 >SPBC428.08c |clr4||histone H3 methyltransferase Clr4|Schizosaccharomyces pombe|chr 2|||Manual Length = 490 Score = 29.9 bits (64), Expect = 0.36 Identities = 10/20 (50%), Positives = 13/20 (65%) Frame = -1 Query: 488 CDCLNTVGCDCLNPVGCDCL 429 C+C + GCD NP C+CL Sbjct: 260 CNCSSLGGCDLNNPSRCECL 279 Score = 25.8 bits (54), Expect = 5.9 Identities = 9/21 (42%), Positives = 12/21 (57%) Frame = -1 Query: 467 GCDCLNPVGCDCLKTVGCDCL 405 GC+C + GCD C+CL Sbjct: 259 GCNCSSLGGCDLNNPSRCECL 279 >SPBC32H8.02c |nep2|mug120|nedd8 protease Nep2|Schizosaccharomyces pombe|chr 2|||Manual Length = 415 Score = 27.1 bits (57), Expect = 2.5 Identities = 14/45 (31%), Positives = 24/45 (53%) Frame = +3 Query: 159 PDKFPYHYCLISRTLLINSINTVIVLLVSTVQPHSLTNSIATSQP 293 P+K + I +T + + T+ + + QPHSL S+ +SQP Sbjct: 296 PEKDKKIFSKICKTEELLELPTLSAVTSDSAQPHSLPASMPSSQP 340 >SPAC652.01 |||BC10 family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 108 Score = 25.4 bits (53), Expect = 7.8 Identities = 11/29 (37%), Positives = 17/29 (58%) Frame = +3 Query: 129 FFALQKSSFLPDKFPYHYCLISRTLLINS 215 F+ + S + K+P YC+I LLIN+ Sbjct: 21 FYPIFMLSMIARKYPCPYCMIILGLLINT 49 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,025,067 Number of Sequences: 5004 Number of extensions: 31776 Number of successful extensions: 77 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 63 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 75 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 317927284 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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