BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060140.seq (686 letters) Database: nematostella 59,808 sequences; 16,821,457 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SB_6824| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.16 SB_23612| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.29 SB_48885| Best HMM Match : DUF741 (HMM E-Value=0.88) 31 0.66 SB_24256| Best HMM Match : Keratin_B2 (HMM E-Value=4.8) 29 2.7 SB_32456| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.1 >SB_6824| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1152 Score = 33.5 bits (73), Expect = 0.16 Identities = 11/41 (26%), Positives = 32/41 (78%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQTF 495 +Y++ + P++++ S+P++++ S+P+ + S+P++F+ S+ F Sbjct: 488 IYDVGK-PSIYELSKPSIYELSKPSIHELSKPSIFELSKPF 527 Score = 31.9 bits (69), Expect = 0.50 Identities = 10/39 (25%), Positives = 31/39 (79%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 +Y +S+ P++F+ S+P++++ +P+ ++ S+P++++ S+ Sbjct: 592 MYELSK-PSIFELSKPSIYELLEPSIYELSKPSIYELSK 629 Score = 31.1 bits (67), Expect = 0.87 Identities = 11/39 (28%), Positives = 30/39 (76%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 +Y +S+ P++++ S+P++ + S+P+ F+ S+P +++ S+ Sbjct: 496 IYELSK-PSIYELSKPSIHELSKPSIFELSKPFIYEVSK 533 Score = 30.7 bits (66), Expect = 1.2 Identities = 9/32 (28%), Positives = 26/32 (81%) Frame = +1 Query: 394 PAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 P++++ S+P++++ S+P ++ S+P++F+ S+ Sbjct: 574 PSIYELSKPSIYELSKPFMYELSKPSIFELSK 605 Score = 30.7 bits (66), Expect = 1.2 Identities = 10/36 (27%), Positives = 29/36 (80%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFK 480 +Y +S+ P++++ S+P +++ S+P+ F+ S+P++++ Sbjct: 576 IYELSK-PSIYELSKPFMYELSKPSIFELSKPSIYE 610 Score = 30.7 bits (66), Expect = 1.2 Identities = 9/34 (26%), Positives = 26/34 (76%) Frame = +1 Query: 394 PAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQTF 495 P++++ S+P++++ S+P+ ++ S+P ++K + F Sbjct: 614 PSIYELSKPSIYELSKPSIYELSKPPIYKLIKPF 647 Score = 30.3 bits (65), Expect = 1.5 Identities = 8/34 (23%), Positives = 27/34 (79%) Frame = +1 Query: 394 PAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQTF 495 P++++ S+P++++ +P+ ++ S+P++++ S+ F Sbjct: 558 PSIYELSKPSIYELLEPSIYELSKPSIYELSKPF 591 Score = 30.3 bits (65), Expect = 1.5 Identities = 11/34 (32%), Positives = 26/34 (76%) Frame = +1 Query: 394 PAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQTF 495 P++++ S+P+VF+ +P+ + S+P++F+ S+ F Sbjct: 670 PSIYELSKPSVFELIKPSINELSKPSIFELSKPF 703 Score = 29.9 bits (64), Expect = 2.0 Identities = 10/36 (27%), Positives = 28/36 (77%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFK 480 +Y +S+ P++ + S+P++F+ S+P ++ S+P++++ Sbjct: 504 IYELSK-PSIHELSKPSIFELSKPFIYEVSKPSIYE 538 Score = 29.9 bits (64), Expect = 2.0 Identities = 10/39 (25%), Positives = 30/39 (76%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 +Y +S+ P +++ S+P++F+ S+P+ ++ +P++++ S+ Sbjct: 584 IYELSK-PFMYELSKPSIFELSKPSIYELLEPSIYELSK 621 Score = 29.5 bits (63), Expect = 2.7 Identities = 11/39 (28%), Positives = 27/39 (69%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 L N R P+V++ S+P++++ S+P + +P++++ S+ Sbjct: 463 LENGFRQPSVYEVSKPSIYEISKPYIYDVGKPSIYELSK 501 Score = 29.5 bits (63), Expect = 2.7 Identities = 10/41 (24%), Positives = 29/41 (70%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQTF 495 +Y +S+ P++++ S+P++++ S+P +K +P +++ + F Sbjct: 616 IYELSK-PSIYELSKPSIYELSKPPIYKLIKPFIYEVIKPF 655 Score = 29.1 bits (62), Expect = 3.5 Identities = 8/39 (20%), Positives = 31/39 (79%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 ++ +S+ P++++ +P++++ S+P+ ++ S+P++++ S+ Sbjct: 600 IFELSK-PSIYELLEPSIYELSKPSIYELSKPSIYELSK 637 Score = 28.7 bits (61), Expect = 4.7 Identities = 9/39 (23%), Positives = 29/39 (74%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 +Y +S+ P++++ S+P ++ +P+ ++ S+P++++ S+ Sbjct: 472 VYEVSK-PSIYEISKPYIYDVGKPSIYELSKPSIYELSK 509 Score = 28.7 bits (61), Expect = 4.7 Identities = 9/39 (23%), Positives = 30/39 (76%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 ++ +S+ P++F+ S+P +++ S+P+ ++ +P++++ S+ Sbjct: 512 IHELSK-PSIFELSKPFIYEVSKPSIYELLEPSIYELSK 549 Score = 28.7 bits (61), Expect = 4.7 Identities = 9/39 (23%), Positives = 30/39 (76%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 +Y +S+ P++++ +P++++ S+P+ ++ S+P +++ S+ Sbjct: 560 IYELSK-PSIYELLEPSIYELSKPSIYELSKPFMYELSK 597 Score = 28.7 bits (61), Expect = 4.7 Identities = 11/38 (28%), Positives = 28/38 (73%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQS 486 +Y +S+ P+VF+ +P++ + S+P+ F+ S+P +++ + Sbjct: 672 IYELSK-PSVFELIKPSINELSKPSIFELSKPFIYEDA 708 Score = 28.3 bits (60), Expect = 6.2 Identities = 10/39 (25%), Positives = 28/39 (71%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFKQSQ 489 +Y IS+ P ++ +P++++ S+P+ ++ S+P++ + S+ Sbjct: 480 IYEISK-PYIYDVGKPSIYELSKPSIYELSKPSIHELSK 517 Score = 27.9 bits (59), Expect = 8.1 Identities = 9/36 (25%), Positives = 27/36 (75%) Frame = +1 Query: 373 LYNISRSPAVFKQSQPTVFKQSQPTGFKQSQPTVFK 480 +Y + + P +++ S+P++++ +P+ ++ S+P+VF+ Sbjct: 648 IYEVIK-PFIYEVSKPSIYELLEPSIYELSKPSVFE 682 >SB_23612| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 1021 Score = 32.7 bits (71), Expect = 0.29 Identities = 19/49 (38%), Positives = 30/49 (61%), Gaps = 1/49 (2%) Frame = +1 Query: 403 FKQSQPTVFKQSQPTGF-KQSQPTVFKQSQTFGRQERVXSSVSAKTTWQ 546 F Q +P F+Q+Q GF +Q QP+ +Q Q F RQ+R+ ++ + T Q Sbjct: 706 FDQFRPQ-FQQAQYPGFPQQQQPSQSQQQQFFPRQQRMMTTTAHSPTAQ 753 >SB_48885| Best HMM Match : DUF741 (HMM E-Value=0.88) Length = 841 Score = 31.5 bits (68), Expect = 0.66 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLKTAGLRE 384 DC V DC V DC K V DC K LR+ Sbjct: 225 DCCKEVKYDCCKDVTNDCCKEVKYDCCKDLTLRK 258 Score = 31.5 bits (68), Expect = 0.66 Identities = 15/34 (44%), Positives = 16/34 (47%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLKTAGLRE 384 DC V DC V DC K V DC K LR+ Sbjct: 380 DCCKKVKYDCCKDVTNDCCKEVKYDCCKDLTLRK 413 Score = 29.5 bits (63), Expect = 2.7 Identities = 13/28 (46%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC V DC V DC K V DC K Sbjct: 276 DCCKEVKYDCCKEVKYDCCKEVKYDCCK 303 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC V DC + DC K V DC K Sbjct: 161 DCCKEVKYDCCKDLTYDCCKEVQNDCCK 188 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC + DC V DC K V DC K Sbjct: 169 DCCKDLTYDCCKEVQNDCCKEVKYDCCK 196 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC V DC + DC K V DC K Sbjct: 209 DCCKEVKYDCCKDLKNDCCKEVKYDCCK 236 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC + DC V DC K V DC K Sbjct: 268 DCCKDLTNDCCKEVKYDCCKEVKYDCCK 295 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC V DC + DC K V DC K Sbjct: 316 DCCKEVKYDCCKDLTYDCCKEVQNDCCK 343 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC + DC V DC K V DC K Sbjct: 324 DCCKDLTYDCCKEVQNDCCKEVKYDCCK 351 Score = 27.9 bits (59), Expect = 8.1 Identities = 12/28 (42%), Positives = 13/28 (46%) Frame = -1 Query: 485 DCLNTVGCDCLNPVGCDCLKTVGCDCLK 402 DC V DC + DC K V DC K Sbjct: 364 DCCKEVKYDCCKDLTNDCCKKVKYDCCK 391 >SB_24256| Best HMM Match : Keratin_B2 (HMM E-Value=4.8) Length = 475 Score = 29.5 bits (63), Expect = 2.7 Identities = 17/53 (32%), Positives = 24/53 (45%), Gaps = 1/53 (1%) Frame = -1 Query: 563 LAFPIICHVVFADTLLXTRS*-RPNVCDCLNTVGCDCLNPVGCDCLKTVGCDC 408 ++ P+I +V ++ TRS RP + DC DC P DC DC Sbjct: 2 VSIPMIMIIV---VMMVTRSCKRPTLVDCTGPTQVDCTGPTLVDCTGPTLVDC 51 >SB_32456| Best HMM Match : No HMM Matches (HMM E-Value=.) Length = 427 Score = 27.9 bits (59), Expect = 8.1 Identities = 13/51 (25%), Positives = 29/51 (56%), Gaps = 1/51 (1%) Frame = +2 Query: 5 TRKSSPMFDI*YTVRTLQIVDYF-IRIVYTVLYALNILLHYQLFCITKKFI 154 T + +F++ Y + + I+ Y+ +I Y + Y I +H++ IT+K++ Sbjct: 284 TTFEADIFNLFYAIVLIFIIGYYPYQIFYILEYTGKISIHWRYLKITRKYL 334 Database: nematostella Posted date: Oct 22, 2007 1:22 PM Number of letters in database: 16,821,457 Number of sequences in database: 59,808 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 15,326,221 Number of Sequences: 59808 Number of extensions: 247784 Number of successful extensions: 836 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 516 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 804 length of database: 16,821,457 effective HSP length: 80 effective length of database: 12,036,817 effective search space used: 1781448916 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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