BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060139.seq (660 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosa... 51 2e-07 SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schiz... 46 4e-06 SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schiz... 31 0.19 SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|c... 28 1.4 SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosac... 27 3.2 SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr 1|||... 26 4.2 SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosacch... 26 5.5 SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|ch... 25 7.3 SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces ... 25 9.7 SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyce... 25 9.7 >SPAC17A5.08 |||COPII-coated vesicle component Erp2/3/4 |Schizosaccharomyces pombe|chr 1|||Manual Length = 210 Score = 50.8 bits (116), Expect = 2e-07 Identities = 29/148 (19%), Positives = 66/148 (44%), Gaps = 5/148 (3%) Frame = +3 Query: 96 LVVTLLNLVSNAYSYTVTVDAHAEECFY-ENVEADTKLGLSFEIAEGGFLDIDV*Y*ALM 272 + + LL +++A T ++ ++C+Y ++ K ++ + GG D+D A Sbjct: 9 ITLFLLASLASAIELTFKLENQEKQCYYLDSFHTGEKTHFTYAVQSGGSFDVDYMIKAPS 68 Query: 273 ATSFIKQRGSHLVFIHFSAPTAGKYTYCFSNKMSSMTPKVVMFNL----EIGEPQTKSGN 440 + + + F+ G+Y +CF N MS+ T K+V + E+ P Sbjct: 69 QKTVALGKKRRQADVFFTLEEKGEYEFCFDNHMSTFTDKIVTMEITMENELSLPALTRDE 128 Query: 441 ENEADHNKLEDMIKELATTLRTINTIKS 524 + + ++ + E++T L I+ +++ Sbjct: 129 AKDYKKDSMQSTVLEISTALSEIDRVQN 156 >SPCC24B10.17 |emp24||COPII-coated vesicle component Emp24 |Schizosaccharomyces pombe|chr 3|||Manual Length = 199 Score = 46.4 bits (105), Expect = 4e-06 Identities = 23/96 (23%), Positives = 45/96 (46%) Frame = +3 Query: 120 VSNAYSYTVTVDAHAEECFYENVEADTKLGLSFEIAEGGFLDIDV*Y*ALMATSFIKQRG 299 +S + + +T+ H ECFYEN+ + ++ ++++ GG + + ++ Sbjct: 16 ISVVFGHGITLKPHQRECFYENLRNNDQMSVTYQTNVGGDQLVSMSIYNPAGQIMHQEVP 75 Query: 300 SHLVFIHFSAPTAGKYTYCFSNKMSSMTPKVVMFNL 407 + + F+ GKY YCF N K V+FN+ Sbjct: 76 NSMAQYSFTVKNPGKYMYCFYNDALDGESKEVLFNV 111 Score = 43.2 bits (97), Expect = 3e-05 Identities = 18/32 (56%), Positives = 21/32 (65%) Frame = +2 Query: 497 IKNYKHDQEYMQVRDRIHRSINESTNSRVVMW 592 I KH+QEY R+RIHR+ ESTN RV W Sbjct: 139 ISKVKHEQEYFVARERIHRNTAESTNDRVKWW 170 >SPAC23H4.03c |erv25||COPII-coated vesicle component Erv25 |Schizosaccharomyces pombe|chr 1|||Manual Length = 216 Score = 30.7 bits (66), Expect = 0.19 Identities = 14/36 (38%), Positives = 21/36 (58%) Frame = +2 Query: 494 YIKNYKHDQEYMQVRDRIHRSINESTNSRVVMWVYI 601 +I+ K Y+Q R+ R+ NESTN RV + Y+ Sbjct: 154 FIEEIKGKIYYLQAREARFRNTNESTNERVKNFAYL 189 >SPCC4B3.04c |nte1||lysophospholipase|Schizosaccharomyces pombe|chr 3|||Manual Length = 1316 Score = 27.9 bits (59), Expect = 1.4 Identities = 13/37 (35%), Positives = 21/37 (56%) Frame = -2 Query: 518 DRVYSS*CSSKFFDHVLKFIMVSFIFITRFSLWFSDF 408 DR+ + SK VL+ + +S + +TRFSL+ F Sbjct: 5 DRILQNRIVSKVIFFVLRSVTLSLVSVTRFSLFLLSF 41 >SPBC16E9.09c |||COPII vesicle coat component Erp5/Erp6|Schizosaccharomyces pombe|chr 2|||Manual Length = 215 Score = 26.6 bits (56), Expect = 3.2 Identities = 11/29 (37%), Positives = 16/29 (55%) Frame = +2 Query: 515 DQEYMQVRDRIHRSINESTNSRVVMWVYI 601 +Q + R+ R +ES NSRVV W + Sbjct: 161 EQMLQRSREMTFRDTSESANSRVVRWTIV 189 >SPAC1A6.03c |||phospholipase |Schizosaccharomyces pombe|chr 1|||Manual Length = 624 Score = 26.2 bits (55), Expect = 4.2 Identities = 17/37 (45%), Positives = 22/37 (59%) Frame = -3 Query: 232 PSAISNDNPNLVSASTFS*KHSSACASTVTV*LYALL 122 PSA+SN P ++SAST S SS A + L+A L Sbjct: 582 PSAVSNYAPTVLSASTTS-GTSSVRAKPIVFYLFASL 617 >SPAC2G11.02 |urb2||ribosome biogenesis protein Urb2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1318 Score = 25.8 bits (54), Expect = 5.5 Identities = 10/26 (38%), Positives = 16/26 (61%) Frame = -3 Query: 133 YALLTRFNKVTTSHCDILKYFILINV 56 YA+L N +T++ CD Y +L+ V Sbjct: 1026 YAILNILNLLTSNECDEFDYSVLMKV 1051 >SPAC3C7.04 |||transcription factor |Schizosaccharomyces pombe|chr 1|||Manual Length = 783 Score = 25.4 bits (53), Expect = 7.3 Identities = 10/21 (47%), Positives = 16/21 (76%) Frame = +3 Query: 453 DHNKLEDMIKELATTLRTINT 515 DH+KL+D +++ A+TL I T Sbjct: 723 DHSKLDDDLEKFASTLDPIKT 743 >SPCC962.01 ||SPCP31B10.09|C2 domain protein|Schizosaccharomyces pombe|chr 3|||Manual Length = 1429 Score = 25.0 bits (52), Expect = 9.7 Identities = 9/21 (42%), Positives = 16/21 (76%) Frame = -3 Query: 253 TSISKKPPSAISNDNPNLVSA 191 TSI+++PPS + + +LV+A Sbjct: 47 TSIARRPPSTVGSQTGSLVNA 67 >SPBC3E7.08c |rad13||DNA repair nuclease Rad13|Schizosaccharomyces pombe|chr 2|||Manual Length = 1112 Score = 25.0 bits (52), Expect = 9.7 Identities = 17/54 (31%), Positives = 30/54 (55%), Gaps = 4/54 (7%) Frame = +3 Query: 345 YTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNEN----EADHNKLEDMIKELAT 494 Y ++ SM P+ ++ ++ E + S +EN EAD+N +D+I +LAT Sbjct: 352 YDVPLPSRSHSMNPRELVA-AKLKEIKENSFSENQQSDEADYNVTDDLILQLAT 404 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,688,201 Number of Sequences: 5004 Number of extensions: 55472 Number of successful extensions: 154 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 147 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 153 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 299817502 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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