BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060139.seq (660 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contain... 37 0.014 At4g10360.1 68417.m01701 expressed protein 32 0.39 At3g02430.1 68416.m00230 hypothetical protein contains Pfam prof... 29 3.6 At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar ... 28 6.3 At1g06660.1 68414.m00706 expressed protein 28 6.3 >At3g22845.1 68416.m02879 emp24/gp25L/p24 protein-related contains weak similarity to transmembrane protein (GI:1212965) [Homo sapiens] Length = 214 Score = 36.7 bits (81), Expect = 0.014 Identities = 39/141 (27%), Positives = 59/141 (41%), Gaps = 7/141 (4%) Frame = +3 Query: 108 LLNLVSNAYSYTVTVDAHAEECFYENV--EADTKLGLSFEIAEGGFLDID---V*Y*ALM 272 LLN ++ S +VTV+ EEC E V E DT G + F D + + Sbjct: 18 LLNSINQISSLSVTVND--EECVQEYVLYEGDTVSGNFVVVDHDIFWGSDHPGLDFTVTS 75 Query: 273 ATSFIKQ--RGSHLVFIHFSAPTAGKYTYCFSNKMSSMTPKVVMFNLEIGEPQTKSGNEN 446 I Q +G+ F AP +G Y +CF N S TP+ V F + +G + Sbjct: 76 PAGNIVQTLKGTSGDKFEFKAPKSGMYKFCFHNPYS--TPETVSFYIHVGHIPNEHDLAK 133 Query: 447 EADHNKLEDMIKELATTLRTI 509 + + + I EL L ++ Sbjct: 134 DEHLDPVNVKIAELREALESV 154 Score = 32.3 bits (70), Expect = 0.29 Identities = 12/26 (46%), Positives = 18/26 (69%) Frame = +2 Query: 515 DQEYMQVRDRIHRSINESTNSRVVMW 592 +Q+Y++ RD HR NEST RV+ + Sbjct: 157 EQKYLKARDTRHRHTNESTRKRVIFY 182 >At4g10360.1 68417.m01701 expressed protein Length = 266 Score = 31.9 bits (69), Expect = 0.39 Identities = 24/93 (25%), Positives = 44/93 (47%) Frame = -2 Query: 590 TSPHVNLYFHLLNDGYDHAPAYTLDRVYSS*CSSKFFDHVLKFIMVSFIFITRFSLWFSD 411 T+P VNL ++L N G + AYTL+ + + F ++ +++ F L F Sbjct: 164 TTPFVNLRWYLDNSGQKGSKAYTLNGI------ALFLGWLVARVLLFIFFFVHMYLHFHQ 217 Query: 410 FKIEHYHLGCHG*HFITEAISILPCSWSRKMYK 312 K + + LG + I A++++ W K+ K Sbjct: 218 VK-QVFPLGFYSLLTIPPALAVMNLLWFWKITK 249 >At3g02430.1 68416.m00230 hypothetical protein contains Pfam profile PF05078: Protein of unknown function (DUF679) Length = 219 Score = 28.7 bits (61), Expect = 3.6 Identities = 17/45 (37%), Positives = 24/45 (53%), Gaps = 1/45 (2%) Frame = +3 Query: 72 KY-LRISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADTK 203 KY +R W+ TL LV A + D + +CFY + EA+TK Sbjct: 139 KYRMRFVDWIHATLSVLVFGAVALR---DKYITDCFYPSPEAETK 180 >At3g10780.1 68416.m01298 emp24/gp25L/p24 family protein similar to SP|O35587 Transmembrane protein Tmp21 precursor (21 kDa Transmembrane trafficking protein) {Mesocricetus auratus}; contains Pfam profile: PF01105 emp24/gp25L/p24 family; contains non-consensus TG acceptor splice site at exon 3 Length = 217 Score = 27.9 bits (59), Expect = 6.3 Identities = 12/35 (34%), Positives = 21/35 (60%) Frame = +2 Query: 479 QRTCYYIKNYKHDQEYMQVRDRIHRSINESTNSRV 583 +R+ Y + + Y+++R+ R INE TN+RV Sbjct: 151 RRSTEYASAIRANILYLRIREAYMREINEKTNTRV 185 >At1g06660.1 68414.m00706 expressed protein Length = 481 Score = 27.9 bits (59), Expect = 6.3 Identities = 14/40 (35%), Positives = 22/40 (55%) Frame = +3 Query: 81 RISQWLVVTLLNLVSNAYSYTVTVDAHAEECFYENVEADT 200 RI V ++ N++ NA Y V D+H + E +EA+T Sbjct: 290 RIRSQFVHSVSNIMENASLYKVYKDSHEGLDYEEQIEAET 329 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,593,178 Number of Sequences: 28952 Number of extensions: 271502 Number of successful extensions: 688 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 672 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 688 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1383534864 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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