BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060138.seq (686 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g18600.1 68416.m02364 DEAD/DEAH box helicase, putative non-co... 39 0.003 At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contai... 38 0.008 At5g08620.1 68418.m01025 DEAD box RNA helicase (RH25) identical ... 35 0.058 At1g55150.1 68414.m06298 DEAD box RNA helicase, putative (RH20) ... 35 0.058 At5g63120.2 68418.m07924 ethylene-responsive DEAD box RNA helica... 33 0.23 At5g63120.1 68418.m07925 ethylene-responsive DEAD box RNA helica... 33 0.23 At5g54910.1 68418.m06839 DEAD/DEAH box helicase, putative 32 0.41 At5g63630.1 68418.m07989 DEAD box RNA helicase, putative strong ... 31 0.54 At3g01540.3 68416.m00084 DEAD box RNA helicase (DRH1) identical ... 31 0.54 At3g01540.2 68416.m00083 DEAD box RNA helicase (DRH1) identical ... 31 0.54 At3g01540.1 68416.m00082 DEAD box RNA helicase (DRH1) identical ... 31 0.54 At5g08610.1 68418.m01024 DEAD box RNA helicase (RH26) strong sim... 31 0.72 At1g51380.1 68414.m05780 eukaryotic translation initiation facto... 30 1.3 At1g31970.1 68414.m03931 DEAD/DEAH box helicase, putative simila... 30 1.7 At3g19760.1 68416.m02501 eukaryotic translation initiation facto... 29 2.2 At3g16840.1 68416.m02150 DEAD/DEAH box helicase, putative (RH13)... 29 2.9 At2g47330.1 68415.m05908 DEAD/DEAH box helicase, putative simila... 29 2.9 At4g33370.1 68417.m04744 DEAD-box protein abstrakt, putative RNA... 29 3.8 At3g43530.1 68416.m04621 hypothetical protein contains Pfam prof... 29 3.8 At4g00660.2 68417.m00091 DEAD/DEAH box helicase, putative simila... 28 5.0 At4g00660.1 68417.m00090 DEAD/DEAH box helicase, putative simila... 28 5.0 At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative simila... 28 5.0 At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar... 28 5.0 At5g51280.1 68418.m06357 DEAD-box protein abstrakt, putative 28 6.7 At4g16630.1 68417.m02514 DEAD/DEAH box helicase, putative (RH28)... 28 6.7 At2g07750.1 68415.m01003 DEAD box RNA helicase, putative similar... 28 6.7 At5g19210.2 68418.m02287 DEAD/DEAH box helicase, putative EUKARY... 27 8.8 At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar... 27 8.8 At1g57943.1 68414.m06569 purine permease-related low similarity ... 27 8.8 >At3g18600.1 68416.m02364 DEAD/DEAH box helicase, putative non-consensus acceptor splice site AT at exon 2; similar to DEAD box helicase protein GB:NP_006764 from [Homo sapiens], contains Pfam profile: PF00270 DEAD/DEAH box helicase Length = 568 Score = 39.1 bits (87), Expect = 0.003 Identities = 21/58 (36%), Positives = 36/58 (62%) Frame = +1 Query: 418 GILSDQKFTALEGTVCEPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 GI+++ F +L+ + E T + IK+MGF + + +G+D++GAA+TGSGK Sbjct: 84 GIMTNVTFDSLD--LSEQTSIAIKEMGFQYMTQIQAGSIQPLLEGKDVLGAARTGSGK 139 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/25 (56%), Positives = 15/25 (60%) Frame = +2 Query: 587 GKTLAFLIPGYRPYIQT*NFXPRNG 661 GKTLAFLIP + F PRNG Sbjct: 138 GKTLAFLIPAVELLFKE-RFSPRNG 161 >At5g65900.1 68418.m08295 DEAD/DEAH box helicase, putative contains Pfam profiles PF00270:DEAD/DEAH box helicase and PF00271: Helicase conserved C-terminal domain; identical to cDNA RH27 helicase, partial GI:4033334 Length = 633 Score = 37.5 bits (83), Expect = 0.008 Identities = 20/57 (35%), Positives = 34/57 (59%) Frame = +1 Query: 421 ILSDQKFTALEGTVCEPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 I++++ F +L ++ + T IK+MGF + + K G D++GAA+TGSGK Sbjct: 150 IMTNKTFESL--SLSDNTYKSIKEMGFARMTQIQAKAIPPLMMGEDVLGAARTGSGK 204 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/25 (56%), Positives = 15/25 (60%) Frame = +2 Query: 587 GKTLAFLIPGYRPYIQT*NFXPRNG 661 GKTLAFLIP + F PRNG Sbjct: 203 GKTLAFLIPAVELLYRV-KFTPRNG 226 >At5g08620.1 68418.m01025 DEAD box RNA helicase (RH25) identical to RNA helicase [Arabidopsis thaliana] GI:3776023; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 563 Score = 34.7 bits (76), Expect = 0.058 Identities = 17/43 (39%), Positives = 25/43 (58%) Frame = +1 Query: 472 TLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTS 600 TL GI+D GF T + L +G+D++ AKTG+GK + Sbjct: 91 TLKGIEDAGFKTMTVVQEATLPLILQGKDILAKAKTGTGKTVA 133 >At1g55150.1 68414.m06298 DEAD box RNA helicase, putative (RH20) similar to ethylene-responsive RNA helicase GI:5669638 from [Lycopersicon esculentum]; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 501 Score = 34.7 bits (76), Expect = 0.058 Identities = 16/39 (41%), Positives = 23/39 (58%) Frame = +1 Query: 484 IKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTS 600 +K GF + + + + KGRDL+G A+TGSGK S Sbjct: 114 VKKAGFTEPTPIQSQGWPMAMKGRDLIGIAETGSGKTLS 152 >At5g63120.2 68418.m07924 ethylene-responsive DEAD box RNA helicase, putative (RH30) strong similarity to ethylene-responsive RNA helicase [Lycopersicon esculentum] GI:5669638; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 591 Score = 32.7 bits (71), Expect = 0.23 Identities = 16/39 (41%), Positives = 23/39 (58%) Frame = +1 Query: 475 LLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 L I +GF + + + + KGRDL+G A+TGSGK Sbjct: 177 LEAIAKLGFTEPTPIQAQGWPMALKGRDLIGIAETGSGK 215 >At5g63120.1 68418.m07925 ethylene-responsive DEAD box RNA helicase, putative (RH30) strong similarity to ethylene-responsive RNA helicase [Lycopersicon esculentum] GI:5669638; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 484 Score = 32.7 bits (71), Expect = 0.23 Identities = 16/39 (41%), Positives = 23/39 (58%) Frame = +1 Query: 475 LLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 L I +GF + + + + KGRDL+G A+TGSGK Sbjct: 177 LEAIAKLGFTEPTPIQAQGWPMALKGRDLIGIAETGSGK 215 >At5g54910.1 68418.m06839 DEAD/DEAH box helicase, putative Length = 739 Score = 31.9 bits (69), Expect = 0.41 Identities = 18/53 (33%), Positives = 28/53 (52%) Frame = +1 Query: 433 QKFTALEGTVCEPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 +KF L + + T G+KD ++ + GRD++GAA+TGSGK Sbjct: 71 RKFAQLP--ISDKTKRGLKDAKYVDMTDVQSAAIPHALCGRDILGAARTGSGK 121 >At5g63630.1 68418.m07989 DEAD box RNA helicase, putative strong similarity to RNA helicase RH25 [Arabidopsis thaliana] GI:3776023; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain; identical to cDNA DEAD box RNA helicase, RH31 GI:3776030 Length = 522 Score = 31.5 bits (68), Expect = 0.54 Identities = 15/43 (34%), Positives = 24/43 (55%) Frame = +1 Query: 472 TLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTS 600 +L IKD G+ T + + KG+D++ AKTG+GK + Sbjct: 65 SLKAIKDAGYETMTVVQEATLPIILKGKDVLAKAKTGTGKTVA 107 >At3g01540.3 68416.m00084 DEAD box RNA helicase (DRH1) identical to RNA helicase DRH1 GB:BAA28347 GI:3149952 [Arabidopsis thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 619 Score = 31.5 bits (68), Expect = 0.54 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +1 Query: 445 ALEGTVCEPTLLG-IKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 + E T P LL + GF + + + + +GRD+V AKTGSGK Sbjct: 159 SFEATGFPPELLREVLSAGFSAPTPIQAQSWPIAMQGRDIVAIAKTGSGK 208 >At3g01540.2 68416.m00083 DEAD box RNA helicase (DRH1) identical to RNA helicase DRH1 GB:BAA28347 GI:3149952 [Arabidopsis thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 619 Score = 31.5 bits (68), Expect = 0.54 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +1 Query: 445 ALEGTVCEPTLLG-IKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 + E T P LL + GF + + + + +GRD+V AKTGSGK Sbjct: 159 SFEATGFPPELLREVLSAGFSAPTPIQAQSWPIAMQGRDIVAIAKTGSGK 208 >At3g01540.1 68416.m00082 DEAD box RNA helicase (DRH1) identical to RNA helicase DRH1 GB:BAA28347 GI:3149952 [Arabidopsis thaliana]; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 618 Score = 31.5 bits (68), Expect = 0.54 Identities = 18/50 (36%), Positives = 26/50 (52%), Gaps = 1/50 (2%) Frame = +1 Query: 445 ALEGTVCEPTLLG-IKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 + E T P LL + GF + + + + +GRD+V AKTGSGK Sbjct: 159 SFEATGFPPELLREVLSAGFSAPTPIQAQSWPIAMQGRDIVAIAKTGSGK 208 >At5g08610.1 68418.m01024 DEAD box RNA helicase (RH26) strong similarity to RNA helicase RH26 [Arabidopsis thaliana] GI:3776025; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain; identical to cDNA DEAD box RNA helicase, RH26 GI:3776024 Length = 850 Score = 31.1 bits (67), Expect = 0.72 Identities = 15/43 (34%), Positives = 24/43 (55%) Frame = +1 Query: 472 TLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTS 600 +L IKD GF T + + +G+D++ AKTG+GK + Sbjct: 393 SLKAIKDAGFETMTVVQEATLPIILQGKDVLAKAKTGTGKTVA 435 >At1g51380.1 68414.m05780 eukaryotic translation initiation factor 4A, putative / eIF-4A, putative Length = 392 Score = 30.3 bits (65), Expect = 1.3 Identities = 12/46 (26%), Positives = 25/46 (54%) Frame = +1 Query: 466 EPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTSV 603 + L G+ D G+ + + + KGRD++ A++G+GK + + Sbjct: 31 DKVLRGVYDYGYKKPSEIQQRALVPILKGRDVIAQAQSGTGKTSMI 76 >At1g31970.1 68414.m03931 DEAD/DEAH box helicase, putative similar to p68 RNA helicase [Schizosaccharomyces pombe] GI:173419 Length = 537 Score = 29.9 bits (64), Expect = 1.7 Identities = 12/14 (85%), Positives = 13/14 (92%) Frame = +1 Query: 550 GRDLVGAAKTGSGK 591 GRDL+G AKTGSGK Sbjct: 151 GRDLIGIAKTGSGK 164 >At3g19760.1 68416.m02501 eukaryotic translation initiation factor 4A, putative / eIF-4A, putative / DEAD box RNA helicase, putative contains DEAD/DEAH helicase domain; similar to RNA helicase GB:CAA09195 from [Arabidopsis thaliana]; identical to cDNA DEAD box RNA helicase, RH2 GI:3775984 Length = 408 Score = 29.5 bits (63), Expect = 2.2 Identities = 12/46 (26%), Positives = 25/46 (54%) Frame = +1 Query: 466 EPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTSV 603 E L G+ + GF + + + +GRD++ A++G+GK + + Sbjct: 44 EDVLRGVYEYGFEKPSAIQQRAVMPILQGRDVIAQAQSGTGKTSMI 89 >At3g16840.1 68416.m02150 DEAD/DEAH box helicase, putative (RH13) similar to RNA helicase GB:CAA09204 from [Arabidopsis thaliana]; identical to cDNA DEAD box RNA helicase, RH13 GI:3776002 Length = 832 Score = 29.1 bits (62), Expect = 2.9 Identities = 10/16 (62%), Positives = 16/16 (100%) Frame = +1 Query: 544 WKGRDLVGAAKTGSGK 591 ++G+D++GAA+TGSGK Sbjct: 226 YQGKDVIGAAETGSGK 241 >At2g47330.1 68415.m05908 DEAD/DEAH box helicase, putative similar to RNA helicase [Rattus norvegicus] GI:897915; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 760 Score = 29.1 bits (62), Expect = 2.9 Identities = 11/17 (64%), Positives = 14/17 (82%) Frame = +1 Query: 550 GRDLVGAAKTGSGKNTS 600 GRD++G AKTGSGK + Sbjct: 265 GRDVIGIAKTGSGKTAA 281 >At4g33370.1 68417.m04744 DEAD-box protein abstrakt, putative RNA helicase DBP2 - Saccharomyces cerevisiae, PID:g5272 Length = 542 Score = 28.7 bits (61), Expect = 3.8 Identities = 15/41 (36%), Positives = 22/41 (53%) Frame = +1 Query: 469 PTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 P L +KD G + + + + GRD++G A TGSGK Sbjct: 107 PLLRMLKDKGIMHPTPIQVQGLPVVLSGRDMIGIAFTGSGK 147 >At3g43530.1 68416.m04621 hypothetical protein contains Pfam profile PF03384: Drosophila protein of unknown function, DUF287 Length = 615 Score = 28.7 bits (61), Expect = 3.8 Identities = 14/28 (50%), Positives = 19/28 (67%) Frame = +2 Query: 164 PEPEKDIEALNNEKEAKKRVGQDGDTNG 247 PE EKDIE ++EK+ K V QD + +G Sbjct: 528 PESEKDIEVADSEKD--KEVPQDDEMDG 553 >At4g00660.2 68417.m00091 DEAD/DEAH box helicase, putative similar to ATP-dependent RNA helicases Length = 505 Score = 28.3 bits (60), Expect = 5.0 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +1 Query: 475 LLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTS 600 L+GI + GF + + + GRD++ AK G+GK + Sbjct: 143 LMGIYEKGFERPSPIQEESIPIALTGRDILARAKNGTGKTAA 184 >At4g00660.1 68417.m00090 DEAD/DEAH box helicase, putative similar to ATP-dependent RNA helicases Length = 505 Score = 28.3 bits (60), Expect = 5.0 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +1 Query: 475 LLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTS 600 L+GI + GF + + + GRD++ AK G+GK + Sbjct: 143 LMGIYEKGFERPSPIQEESIPIALTGRDILARAKNGTGKTAA 184 >At3g09620.1 68416.m01141 DEAD/DEAH box helicase, putative similar to RNA helicase GB:A57514 GI:897915 from [Rattus norvegicus]; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 989 Score = 28.3 bits (60), Expect = 5.0 Identities = 11/14 (78%), Positives = 12/14 (85%) Frame = +1 Query: 550 GRDLVGAAKTGSGK 591 GRD +G AKTGSGK Sbjct: 433 GRDCIGVAKTGSGK 446 >At1g20920.1 68414.m02619 DEAD box RNA helicase, putative similar to RNA helicase [Rattus norvegicus] GI:897915; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 1166 Score = 28.3 bits (60), Expect = 5.0 Identities = 11/14 (78%), Positives = 12/14 (85%) Frame = +1 Query: 550 GRDLVGAAKTGSGK 591 GRD +G AKTGSGK Sbjct: 566 GRDCIGVAKTGSGK 579 >At5g51280.1 68418.m06357 DEAD-box protein abstrakt, putative Length = 591 Score = 27.9 bits (59), Expect = 6.7 Identities = 14/41 (34%), Positives = 22/41 (53%) Frame = +1 Query: 469 PTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 P L +K+ G + + + + GRD++G A TGSGK Sbjct: 156 PVLDTLKEKGIVQPTPIQVQGLPVILAGRDMIGIAFTGSGK 196 >At4g16630.1 68417.m02514 DEAD/DEAH box helicase, putative (RH28) identical to cDNA DEAD box RNA helicase, RH28 GI:3776026 Length = 789 Score = 27.9 bits (59), Expect = 6.7 Identities = 16/53 (30%), Positives = 24/53 (45%) Frame = +1 Query: 442 TALEGTVCEPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKNTS 600 T +E + P L + +G+ + L GRDL +A TGSGK + Sbjct: 168 TFMELNLSRPLLRACETLGYKKPTPIQAACIPLALTGRDLCASAITGSGKTAA 220 >At2g07750.1 68415.m01003 DEAD box RNA helicase, putative similar to RNA helicase RH25 [Arabidopsis thaliana] GI:3776023; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 845 Score = 27.9 bits (59), Expect = 6.7 Identities = 14/48 (29%), Positives = 22/48 (45%) Frame = +1 Query: 451 EGTVCEPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKN 594 E + TL + G + R + C G+D + AKTG+GK+ Sbjct: 379 ESCISPLTLKALSASGIVKMTRVQDATLSECLDGKDALVKAKTGTGKS 426 >At5g19210.2 68418.m02287 DEAD/DEAH box helicase, putative EUKARYOTIC INITIATION FACTOR 4A-II (EIF-4A-II), Homo sapiens, SWISSPROT:IF42_HUMAN Length = 472 Score = 27.5 bits (58), Expect = 8.8 Identities = 15/47 (31%), Positives = 26/47 (55%) Frame = +1 Query: 451 EGTVCEPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGK 591 +G V E L ++++GF+ + + + GRD + A+TGSGK Sbjct: 80 QGFVPEHILHRMEEIGFVFPTDIQREALPTLFTGRDCILHAQTGSGK 126 >At1g63250.1 68414.m07150 DEAD box RNA helicase, putative similar to RNA helicase (RH25) [Arabidopsis thaliana] GI:3776023; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 798 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/48 (29%), Positives = 22/48 (45%) Frame = +1 Query: 451 EGTVCEPTLLGIKDMGFITRQRFKPKLYLLCWKGRDLVGAAKTGSGKN 594 E + TL + G + R + C G+D + AKTG+GK+ Sbjct: 332 ESCISPLTLKALSASGILKMTRVQDATLSECLDGKDALVKAKTGTGKS 379 >At1g57943.1 68414.m06569 purine permease-related low similarity to purine permease [Arabidopsis thaliana] GI:7620007; contains Pfam profile PF03151: Domain of unknown function, DUF250 Length = 398 Score = 27.5 bits (58), Expect = 8.8 Identities = 12/30 (40%), Positives = 20/30 (66%), Gaps = 1/30 (3%) Frame = -1 Query: 587 PEP-VLAAPTRSLPFQQRRYSFGLNLCLVI 501 PEP + +P RSL +QR++ ++LCL + Sbjct: 22 PEPDQILSPRRSLELKQRKWWISVSLCLFL 51 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,179,516 Number of Sequences: 28952 Number of extensions: 188757 Number of successful extensions: 573 Number of sequences better than 10.0: 29 Number of HSP's better than 10.0 without gapping: 543 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 567 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1457719448 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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