Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060135.seq
         (661 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb...   103   2e-23
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces...    55   8e-09
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce...    51   2e-07
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc...    50   4e-07
SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyce...    31   0.15 
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha...    28   1.0  
SPBC2D10.13 |est1||telomerase regulator Est1|Schizosaccharomyces...    26   5.5  
SPAC4G9.09c |arg11||N-acetyl-gamma-glutamyl-phosphate reductase/...    26   5.5  
SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1...    26   5.5  
SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit L2|Sc...    25   9.7  
SPCC584.04 |sup35|erf3|translation release factor eRF3 |Schizosa...    25   9.7  

>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 923

 Score =  103 bits (247), Expect = 2e-23
 Identities = 44/80 (55%), Positives = 56/80 (70%)
 Frame = +3

Query: 261 WSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETK 440
           W  L  S+P  L+  I+G +F G+DV GFF  P+AEL  RWY+ A F PFFRAH+HI+TK
Sbjct: 605 WEHLRGSIPTVLTNGISGMAFSGADVAGFFGNPDAELFVRWYETAIFYPFFRAHAHIDTK 664

Query: 441 RREPWLYPAVTTALIRDANR 500
           RREPWLY    T+L+R+  R
Sbjct: 665 RREPWLYGEPYTSLVRELLR 684



 Score = 70.1 bits (164), Expect = 3e-13
 Identities = 36/88 (40%), Positives = 46/88 (52%)
 Frame = +1

Query: 13  GPEVTMPKDCRHYKPPQDGLEGLAAYWEHRHVHNEYGLWNLRATNTGLLDRADGVYRPFL 192
           GPE +M +D  HY             WEHR +HN YG   +  T  GL+ R +G  RPF+
Sbjct: 533 GPETSMHRDAIHY-----------GGWEHRDIHNIYGHKCINGTYNGLIKRGEGAVRPFI 581

Query: 193 LTRAVFAGTQRYSAVWTGDNTRSGRSLR 276
           LTR+ FAGT   +A W GD   +   LR
Sbjct: 582 LTRSFFAGTSALAANWIGDTMTTWEHLR 609



 Score = 28.3 bits (60), Expect = 1.0
 Identities = 11/28 (39%), Positives = 14/28 (50%)
 Frame = +2

Query: 512 LLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
           LL  WYT F      G P++ P F  +P
Sbjct: 689 LLPTWYTAFYNSHTHGFPILYPQFLMHP 716


>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 995

 Score = 55.2 bits (127), Expect = 8e-09
 Identities = 25/73 (34%), Positives = 41/73 (56%)
 Frame = +3

Query: 252 YAEWSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHI 431
           Y+ WS +  S+P  L+  + G    G+DV GF    + EL +RW    AF PF+R H+ +
Sbjct: 671 YSLWSNMIFSIPGALTFNMVGLPMVGADVCGFMGNTDEELCSRWMALGAFLPFYRNHNSL 730

Query: 432 ETKRREPWLYPAV 470
            +  +EP+ + +V
Sbjct: 731 GSISQEPYRWESV 743



 Score = 40.7 bits (91), Expect = 2e-04
 Identities = 20/50 (40%), Positives = 28/50 (56%)
 Frame = +1

Query: 103 HVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDN 252
           ++ N YG    R T   L      V RPF+L+R+ F G+ +Y+A W GDN
Sbjct: 622 NLFNTYGYDQSRVTYDSLTSIEPNV-RPFILSRSTFVGSGKYAAHWLGDN 670



 Score = 39.1 bits (87), Expect = 6e-04
 Identities = 15/29 (51%), Positives = 21/29 (72%)
 Frame = +2

Query: 509 ALLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
           +LL +WYTL  E +  GLP++RPLF  +P
Sbjct: 756 SLLPYWYTLMYEASSQGLPLIRPLFFEFP 784


>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 993

 Score = 50.8 bits (116), Expect = 2e-07
 Identities = 23/75 (30%), Positives = 41/75 (54%)
 Frame = +3

Query: 255 AEWSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIE 434
           ++WS + +S+   L+  + G    G+DV G+    + EL  RW    AF PF+R H+ + 
Sbjct: 675 SQWSDMVSSISSILTFNLLGIPMVGADVCGYNGNTDEELCARWMALGAFLPFYRNHNSLG 734

Query: 435 TKRREPWLYPAVTTA 479
           +  +EP+ + +V  A
Sbjct: 735 SIPQEPFRWASVAEA 749



 Score = 39.5 bits (88), Expect = 4e-04
 Identities = 15/29 (51%), Positives = 21/29 (72%)
 Frame = +2

Query: 509 ALLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
           +LL +WYTL    +VDG P++RPLF  +P
Sbjct: 759 SLLPYWYTLMHTASVDGTPMVRPLFFEFP 787



 Score = 38.7 bits (86), Expect = 7e-04
 Identities = 15/24 (62%), Positives = 19/24 (79%)
 Frame = +1

Query: 181 RPFLLTRAVFAGTQRYSAVWTGDN 252
           RPFLL+R+ F G+ RY+A W GDN
Sbjct: 650 RPFLLSRSTFVGSGRYAAHWLGDN 673


>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 969

 Score = 49.6 bits (113), Expect = 4e-07
 Identities = 24/73 (32%), Positives = 38/73 (52%)
 Frame = +3

Query: 261 WSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETK 440
           WS +  S+   +   + G    G+DV GF    + EL +RW    AF PF+R H++I   
Sbjct: 652 WSNMFFSISGMIVFNMMGIPMVGADVCGFLGDSDEELCSRWMAMGAFSPFYRNHNNIYQI 711

Query: 441 RREPWLYPAVTTA 479
            +EP+ + +V  A
Sbjct: 712 SQEPYTWSSVAEA 724



 Score = 35.1 bits (77), Expect = 0.009
 Identities = 17/49 (34%), Positives = 25/49 (51%)
 Frame = +1

Query: 106 VHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDN 252
           + N YG    + +   L   +    RPF+L+R+ F G+  Y A W GDN
Sbjct: 601 IFNMYGYGETKVSYAALTQISPNE-RPFILSRSTFLGSGVYGAHWLGDN 648



 Score = 34.3 bits (75), Expect = 0.016
 Identities = 12/29 (41%), Positives = 19/29 (65%)
 Frame = +2

Query: 509 ALLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
           +LL +WYT+  + + DG P +R LF  +P
Sbjct: 734 SLLPYWYTIMAKASQDGTPALRALFVEFP 762


>SPAC1805.13 |rpl14||60S ribosomal protein L14|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 134

 Score = 31.1 bits (67), Expect = 0.15
 Identities = 13/33 (39%), Positives = 21/33 (63%), Gaps = 2/33 (6%)
 Frame = +2

Query: 203 PCSQAPRDTLRYGQVTIRGVV--VPCGFRADVL 295
           PCS+ PR  +RYG V +  +V  +P G R+ ++
Sbjct: 42  PCSEFPRQVIRYGSVVLTHIVMKLPRGARSGIV 74


>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
           Upf1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 925

 Score = 28.3 bits (60), Expect = 1.0
 Identities = 10/18 (55%), Positives = 12/18 (66%)
 Frame = -1

Query: 265 DHSAYCHLSIPQSISGCL 212
           +H AYCH+  P SI  CL
Sbjct: 42  EHCAYCHIKNPNSILKCL 59


>SPBC2D10.13 |est1||telomerase regulator Est1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 490

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 9/23 (39%), Positives = 16/23 (69%)
 Frame = +1

Query: 295 CHWQ*PGTVSAVLTSEDSSSIPK 363
           C W    TVS+ +++++ SSIP+
Sbjct: 132 CKWTSSATVSSTISNDEMSSIPE 154


>SPAC4G9.09c |arg11||N-acetyl-gamma-glutamyl-phosphate
           reductase/acetylglutamate kinase|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 885

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 15/50 (30%), Positives = 25/50 (50%)
 Frame = -2

Query: 321 NCSRLLPVTSTSARKPQGTTTPRIVTCPYRRVSLGACEHGPREQEGSVDA 172
           N    L ++  S+R+ +GT  P       + V+L   +    E+EG+VDA
Sbjct: 591 NTHPYLELSHVSSRELEGTKLPGYTKKEIQYVNLSTDDVKKLEEEGAVDA 640


>SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1023

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 17/47 (36%), Positives = 24/47 (51%)
 Frame = -3

Query: 251 LSPVHTAEYLWVPANTALVSRKGL*TPSARSSSPVLVARRFHSPYSL 111
           LSP     Y  +P++++  SR G  TPS R  S +L    F S + L
Sbjct: 92  LSPSFNTSYQAIPSSSSNRSRGGPYTPSIRDDS-LLALLSFSSNHRL 137


>SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit
           L2|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 318

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 13/42 (30%), Positives = 20/42 (47%)
 Frame = -1

Query: 373 MSSASGYLKNPPTSEPQKLFPAIASDKHIGTEAARNDHSAYC 248
           + S    LK     EP ++ P +A++   GT AA+N     C
Sbjct: 126 VESFRSLLKTGSNGEPVEMDPVLAAEIEDGTFAAKNLKPGNC 167


>SPCC584.04 |sup35|erf3|translation release factor eRF3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 662

 Score = 25.0 bits (52), Expect = 9.7
 Identities = 8/28 (28%), Positives = 15/28 (53%)
 Frame = +3

Query: 381 WYQAAAFQPFFRAHSHIETKRREPWLYP 464
           WYQ  +   +  + +H+E K   P++ P
Sbjct: 445 WYQGPSLLEYLDSMTHLERKVNAPFIMP 472


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,617,184
Number of Sequences: 5004
Number of extensions: 51212
Number of successful extensions: 181
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 162
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 180
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 299817502
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)