Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060135.seq
(661 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to ... 108 3e-24
At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein si... 66 1e-11
At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical t... 59 2e-09
At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to al... 55 5e-08
At3g45940.1 68416.m04971 alpha-xylosidase, putative strong simil... 51 8e-07
At4g17920.1 68417.m02670 zinc finger (C3HC4-type RING finger) fa... 31 0.68
At4g28610.1 68417.m04091 myb family transcription factor, putati... 30 1.2
At1g02230.1 68414.m00161 no apical meristem (NAM) family protein... 29 2.1
At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family pr... 29 3.6
At3g01420.1 68416.m00065 pathogen-responsive alpha-dioxygenase, ... 28 4.8
At2g25920.1 68415.m03110 expressed protein 28 4.8
At2g29930.3 68415.m03638 F-box family protein contains F-box dom... 28 6.3
At2g29930.2 68415.m03636 F-box family protein contains F-box dom... 28 6.3
At2g29930.1 68415.m03637 F-box family protein contains F-box dom... 28 6.3
At5g51300.2 68418.m06360 splicing factor-related contains simila... 27 8.4
At5g51300.1 68418.m06359 splicing factor-related contains simila... 27 8.4
At1g63650.2 68414.m07202 basic helix-loop-helix (bHLH) family pr... 27 8.4
At1g63650.1 68414.m07201 basic helix-loop-helix (bHLH) family pr... 27 8.4
>At5g63840.1 68418.m08014 alpha-glucosidase, putative similar to
alpha-glucosidase GI:2648032 from [Solanum tuberosum]
Length = 921
Score = 108 bits (260), Expect = 3e-24
Identities = 45/80 (56%), Positives = 57/80 (71%)
Frame = +3
Query: 255 AEWSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIE 434
AEW L S+PM L+L + G +F G+D+GGFF PE EL+ RWYQ A+ PFFR H+H +
Sbjct: 591 AEWEHLRVSIPMILTLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHD 650
Query: 435 TKRREPWLYPAVTTALIRDA 494
TKRREPWL+ T L+RDA
Sbjct: 651 TKRREPWLFGERNTELMRDA 670
Score = 89.4 bits (212), Expect = 2e-18
Identities = 46/90 (51%), Positives = 58/90 (64%)
Frame = +1
Query: 10 NGPEVTMPKDCRHYKPPQDGLEGLAAYWEHRHVHNEYGLWNLRATNTGLLDRADGVYRPF 189
NGPEVTMP+D H + G+ EHR VHN YG + AT+ GL+ R +G RPF
Sbjct: 520 NGPEVTMPRDALH-------VGGV----EHREVHNAYGYYFHMATSDGLVMREEGKDRPF 568
Query: 190 LLTRAVFAGTQRYSAVWTGDNTRSGRSLRL 279
+L+RA+F GTQRY A+WTGDNT LR+
Sbjct: 569 VLSRAIFPGTQRYGAIWTGDNTAEWEHLRV 598
Score = 37.9 bits (84), Expect = 0.006
Identities = 15/28 (53%), Positives = 21/28 (75%)
Frame = +2
Query: 512 LLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
LL ++YTLF E V G+PV+RPL+ +P
Sbjct: 677 LLPYFYTLFREANVTGVPVVRPLWMEFP 704
>At3g23640.1 68416.m02973 glycosyl hydrolase family 31 protein
similar to alpha-glucosidase II from SP:Q9F234 [Bacillus
thermoamyloliquefaciens]; contains Pfam profile: PF01055
Glycosyl hydrolases family 31
Length = 991
Score = 66.5 bits (155), Expect = 1e-11
Identities = 36/85 (42%), Positives = 49/85 (57%)
Frame = +1
Query: 25 TMPKDCRHYKPPQDGLEGLAAYWEHRHVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRA 204
TMP++ H+ G + L H H HN YG+ R+T G+ + AD RPF+LTRA
Sbjct: 358 TMPENNIHH-----GDDELGGVQNHSHYHNVYGMLMARSTYEGM-ELADKNKRPFVLTRA 411
Query: 205 VFAGTQRYSAVWTGDNTRSGRSLRL 279
F G+QRY+A WTGDN + L +
Sbjct: 412 GFIGSQRYAATWTGDNLSNWEHLHM 436
Score = 55.2 bits (127), Expect = 4e-08
Identities = 25/69 (36%), Positives = 31/69 (44%)
Frame = +3
Query: 255 AEWSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIE 434
+ W L S+ M L L ++G G D+GGF L RW A PF R HS
Sbjct: 429 SNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRWMGVGAMFPFCRGHSEAG 488
Query: 435 TKRREPWLY 461
T EPW +
Sbjct: 489 TDDHEPWSF 497
>At5g11720.1 68418.m01369 alpha-glucosidase 1 (AGLU1) identical to
alpha-glucosidase 1 [Arabidopsis thaliana] GI:2323344
Length = 902
Score = 59.3 bits (137), Expect = 2e-09
Identities = 29/75 (38%), Positives = 42/75 (56%)
Frame = +3
Query: 255 AEWSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIE 434
A+W LA S+P L+ + G G+D+ GF EL RW Q AF PF R HS +
Sbjct: 561 AKWEDLAYSIPGILNFGLFGIPMVGADICGFSHDTTEELCRRWIQLGAFYPFARDHSSLG 620
Query: 435 TKRREPWLYPAVTTA 479
T R+E +L+ +V ++
Sbjct: 621 TARQELYLWDSVASS 635
Score = 47.2 bits (107), Expect = 1e-05
Identities = 22/55 (40%), Positives = 32/55 (58%)
Frame = +1
Query: 109 HNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTRSGRSL 273
HN YGL +AT+ ++D RPF+L+R+ F + +Y+A WTGDN L
Sbjct: 514 HNLYGLLEAKATHQAVVDITGK--RPFILSRSTFVSSGKYTAHWTGDNAAKWEDL 566
Score = 29.9 bits (64), Expect = 1.6
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = +2
Query: 512 LLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
LL YTL E V G P+ RPLF +P
Sbjct: 646 LLPHLYTLMYEAHVSGNPIARPLFFSFP 673
>At1g68560.1 68414.m07833 alpha-xylosidase (XYL1) identical to
alpha-xylosidase precursor GB:AAD05539 GI:4163997 from
[Arabidopsis thaliana]; contains Pfam profile PF01055:
Glycosyl hydrolases family 31; identical to cDNA
alpha-xylosidase precursor (XYL1) partial cds GI:4163996
Length = 915
Score = 54.8 bits (126), Expect = 5e-08
Identities = 25/70 (35%), Positives = 36/70 (51%)
Frame = +3
Query: 261 WSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETK 440
W L S+ L+ I G GSD+ GF+ P EL RW + AF PF R H++ +
Sbjct: 568 WQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSRDHANYYSP 627
Query: 441 RREPWLYPAV 470
R+E + + V
Sbjct: 628 RQELYQWDTV 637
Score = 48.8 bits (111), Expect = 3e-06
Identities = 25/57 (43%), Positives = 38/57 (66%)
Frame = +1
Query: 109 HNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTRSGRSLRL 279
H+ YG AT+ GLL+ G RPF+L+R+ F G+ +Y+A WTGDN + +SL++
Sbjct: 519 HSIYGFSETIATHKGLLN-VQGK-RPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQV 573
Score = 29.9 bits (64), Expect = 1.6
Identities = 13/28 (46%), Positives = 17/28 (60%)
Frame = +2
Query: 512 LLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
+L F YTL E + G P+ RPLF +P
Sbjct: 651 ILPFLYTLNYEAHMTGAPIARPLFFSFP 678
>At3g45940.1 68416.m04971 alpha-xylosidase, putative strong
similarity to alpha-xylosidase precursor GI:4163997 from
[Arabidopsis thaliana]
Length = 868
Score = 50.8 bits (116), Expect = 8e-07
Identities = 25/70 (35%), Positives = 33/70 (47%)
Frame = +3
Query: 261 WSFLAASVPMCLSLAIAGNSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETK 440
W L S+ L+ I G GSD+ GFF EL RW + AF PF R H+
Sbjct: 520 WQSLQVSISTMLNFGIFGVPMVGSDICGFFPPTPEELCNRWIEVGAFYPFSRDHADYYAP 579
Query: 441 RREPWLYPAV 470
R+E + + V
Sbjct: 580 RKELYQWGTV 589
Score = 45.6 bits (103), Expect = 3e-05
Identities = 24/57 (42%), Positives = 36/57 (63%)
Frame = +1
Query: 109 HNEYGLWNLRATNTGLLDRADGVYRPFLLTRAVFAGTQRYSAVWTGDNTRSGRSLRL 279
H+ YG AT+ LL A RPF+L+R+ F G+ +Y+A WTGDN + +SL++
Sbjct: 471 HSIYGFSEAIATHKALL--AVQGKRPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQV 525
Score = 31.1 bits (67), Expect = 0.68
Identities = 14/28 (50%), Positives = 17/28 (60%)
Frame = +2
Query: 512 LLDFWYTLFXEHTVDGLPVMRPLFQHYP 595
LL F YTL E + G P+ RPLF +P
Sbjct: 603 LLPFLYTLNYEAHMSGAPIARPLFFSFP 630
>At4g17920.1 68417.m02670 zinc finger (C3HC4-type RING finger)
family protein similar to RING-H2 zinc finger protein
ATL6 [Arabidopsis thaliana] GI:4928403; contains Pfam
profile PF00097: Zinc finger, C3HC4 type (RING finger)
Length = 289
Score = 31.1 bits (67), Expect = 0.68
Identities = 16/43 (37%), Positives = 23/43 (53%)
Frame = -1
Query: 526 PEVEQRYLLRLASRMSAVVTAGYSQGSRRLVSMCEWARKNGWN 398
PE E +Y LRL + VT G+SQ + V+ E R G++
Sbjct: 233 PEEEDKYTLRLPEHVKIKVTRGHSQ-TESCVTFAELIRNRGYD 274
>At4g28610.1 68417.m04091 myb family transcription factor, putative
/ phosphate starvation response regulator, putative
(PHR1) contains Pfam profile: PF00249 myb-like
DNA-binding domain; identical to cDNA phosphate
starvation response regulator 1 (phr1 gene) GI:15384675
Length = 409
Score = 30.3 bits (65), Expect = 1.2
Identities = 22/73 (30%), Positives = 35/73 (47%), Gaps = 5/73 (6%)
Frame = -1
Query: 400 NAAAWYHLVMSSASGYLKNPPTS-----EPQKLFPAIASDKHIGTEAARNDHSAYCHLSI 236
N A H+ SS+SG+ N S E Q+ + +S+ + T + N+ SA+CH S+
Sbjct: 58 NGGAVGHICSSSSSGFATNLHYSTMVSHEKQQHYTGSSSNNAVQTPS--NNDSAWCHDSL 115
Query: 235 PQSISGCLRTRPS 197
P T P+
Sbjct: 116 PGGFLDFHETNPA 128
>At1g02230.1 68414.m00161 no apical meristem (NAM) family protein
contains Pfam PF02365: No apical meristem (NAM) protein
Length = 579
Score = 29.5 bits (63), Expect = 2.1
Identities = 19/50 (38%), Positives = 22/50 (44%), Gaps = 3/50 (6%)
Frame = +3
Query: 315 NSFCGSDVGGFFKYPEAELMTRWYQAAAFQPFFRAHSHIE---TKRREPW 455
NS GS+VG + L T Y F PFFR S I+ T E W
Sbjct: 177 NSSGGSEVGESQTFSVLSLETLKYLTLVFLPFFRGESQIDDATTPIEEEW 226
>At5g41315.1 68418.m05021 basic helix-loop-helix (bHLH) family
protein contains Pfam profile: PF00010 helix-loop-helix
DNA-binding domain ;annotation temporarily based on
supporting cDNA gi|17224394|gb|AF246291.1|AF246291
Length = 637
Score = 28.7 bits (61), Expect = 3.6
Identities = 12/42 (28%), Positives = 24/42 (57%), Gaps = 1/42 (2%)
Frame = +1
Query: 100 RHVHNEYGL-WNLRATNTGLLDRADGVYRPFLLTRAVFAGTQ 222
R++ YG+ W++ A+ +G+L+ DG Y + TR ++
Sbjct: 23 RNIQWSYGIFWSVSASQSGVLEWGDGYYNGDIKTRKTIQASE 64
>At3g01420.1 68416.m00065 pathogen-responsive alpha-dioxygenase,
putative similar to pathogen-inducible alpha-dioxygenase
[Nicotiana attenuata] GI:12539609; contains Pfam profile
PF03098: Animal haem peroxidase
Length = 639
Score = 28.3 bits (60), Expect = 4.8
Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = +1
Query: 76 GLAAYWEHRHVHNEYGLWNLRATNTGL-LDRADGVYR 183
GL RH+H +Y L N+ T TG+ D A+ YR
Sbjct: 55 GLTYLEVRRHLHQQYNLLNVGQTPTGIRFDPANYPYR 91
>At2g25920.1 68415.m03110 expressed protein
Length = 280
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/46 (30%), Positives = 22/46 (47%)
Frame = -2
Query: 303 PVTSTSARKPQGTTTPRIVTCPYRRVSLGACEHGPREQEGSVDAVG 166
P+TST++ +P T TCP +S A P+ ++ D G
Sbjct: 148 PLTSTNSPQPSPTILHHSHTCPASMISNAATTTTPQSRQRGSDTEG 193
>At2g29930.3 68415.m03638 F-box family protein contains F-box domain
Pfam:PF00646
Length = 459
Score = 27.9 bits (59), Expect = 6.3
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +2
Query: 485 PGRQPQEIALLDFWYTLFXEHTVDGLPVMRPLFQHY 592
P + + ++F TL ++ + G PV+ LF HY
Sbjct: 157 PALKSLSLVSVEFTDTLMYQNFISGCPVLEELFLHY 192
>At2g29930.2 68415.m03636 F-box family protein contains F-box domain
Pfam:PF00646
Length = 339
Score = 27.9 bits (59), Expect = 6.3
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +2
Query: 485 PGRQPQEIALLDFWYTLFXEHTVDGLPVMRPLFQHY 592
P + + ++F TL ++ + G PV+ LF HY
Sbjct: 37 PALKSLSLVSVEFTDTLMYQNFISGCPVLEELFLHY 72
>At2g29930.1 68415.m03637 F-box family protein contains F-box domain
Pfam:PF00646
Length = 459
Score = 27.9 bits (59), Expect = 6.3
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +2
Query: 485 PGRQPQEIALLDFWYTLFXEHTVDGLPVMRPLFQHY 592
P + + ++F TL ++ + G PV+ LF HY
Sbjct: 157 PALKSLSLVSVEFTDTLMYQNFISGCPVLEELFLHY 192
>At5g51300.2 68418.m06360 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/37 (32%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = -1
Query: 382 HLVMSSASGYLKNPPTSEPQKLF-PAIASDKHIGTEA 275
H+ + G +NP +SEPQ+ F P + +D T +
Sbjct: 646 HVTQAPPPGTTQNPSSSEPQQSFPPGVQADSGAATSS 682
>At5g51300.1 68418.m06359 splicing factor-related contains
similarity to SF1 protein [Drosophila melanogaster]
GI:6687400
Length = 804
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/37 (32%), Positives = 20/37 (54%), Gaps = 1/37 (2%)
Frame = -1
Query: 382 HLVMSSASGYLKNPPTSEPQKLF-PAIASDKHIGTEA 275
H+ + G +NP +SEPQ+ F P + +D T +
Sbjct: 646 HVTQAPPPGTTQNPSSSEPQQSFPPGVQADSGAATSS 682
>At1g63650.2 68414.m07202 basic helix-loop-helix (bHLH) family
protein similar to bHLH transcription factor (JAF13)
GB:AAC39455 [Petunia x hybrida]; contains Pfam profile:
PF00010 Helix-loop-helix DNA-binding domain
Length = 596
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +1
Query: 100 RHVHNEYGL-WNLRATNTGLLDRADGVYRPFLLTR 201
R++ YG+ W++ A+ G+L+ DG Y + TR
Sbjct: 22 RNIQWSYGIFWSVSASQPGVLEWGDGYYNGDIKTR 56
>At1g63650.1 68414.m07201 basic helix-loop-helix (bHLH) family
protein similar to bHLH transcription factor (JAF13)
GB:AAC39455 [Petunia x hybrida]; contains Pfam profile:
PF00010 Helix-loop-helix DNA-binding domain
Length = 596
Score = 27.5 bits (58), Expect = 8.4
Identities = 12/35 (34%), Positives = 21/35 (60%), Gaps = 1/35 (2%)
Frame = +1
Query: 100 RHVHNEYGL-WNLRATNTGLLDRADGVYRPFLLTR 201
R++ YG+ W++ A+ G+L+ DG Y + TR
Sbjct: 22 RNIQWSYGIFWSVSASQPGVLEWGDGYYNGDIKTR 56
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,645,855
Number of Sequences: 28952
Number of extensions: 302931
Number of successful extensions: 943
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 902
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 940
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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