Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060134.seq
(685 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1 |Schizosacc... 71 1e-13
SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4 |Schizosac... 66 3e-12
SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3 |Schizosacc... 36 0.005
SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2 |Schizosacc... 29 0.63
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 28 1.1
SPBC3H7.15 |hhp1||serine/threonine protein kinase Hhp1|Schizosac... 27 2.5
SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3 |Schizosacc... 26 5.8
SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces... 25 7.7
>SPBP16F5.07 |apm1||AP-1 adaptor complex subunit Apm1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 426
Score = 71.3 bits (167), Expect = 1e-13
Identities = 35/91 (38%), Positives = 56/91 (61%)
Frame = +2
Query: 239 IQSECVHARQQXRSPVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIXDVMQS 418
++SE V Q +P ++ +I +++L A++K N +A + FL K+ DV
Sbjct: 35 LKSE-VEEEQGFSTPCLTHEGINYIYIHHNDVYLLALSKMNSDAMEMLVFLRKMADVFID 93
Query: 419 YFGKISEEXXXNNFVLIYELLDEILDFGYPR 511
YF ++ EE +NFVL+YELLDEI+DFG+P+
Sbjct: 94 YFKELQEESIRDNFVLVYELLDEIMDFGFPQ 124
Score = 34.3 bits (75), Expect = 0.017
Identities = 16/32 (50%), Positives = 21/32 (65%)
Frame = +3
Query: 147 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAF 242
M +FV N KG+V+ISR YR DI + V+ F
Sbjct: 1 MASAIFVLNLKGKVIISRDYRADIPMSVVEKF 32
>SPAC31A2.09c |apm4||AP-2 adaptor complex subunit Apm4
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 446
Score = 66.5 bits (155), Expect = 3e-12
Identities = 27/76 (35%), Positives = 48/76 (63%)
Frame = +2
Query: 275 RSPVTNIARTSFFHIKRANIWLAAVTKQNVNAAMVFEFLLKIXDVMQSYFGKISEEXXXN 454
R P+ +I +++ + K ++++ A+TK N N +V EFL + + YFGK++E +
Sbjct: 43 RHPIVSIGSSTYIYTKHEDLYVVAITKGNPNVMIVLEFLESLIQDLTHYFGKLNENTVKD 102
Query: 455 NFVLIYELLDEILDFG 502
N I+ELLDE++D+G
Sbjct: 103 NVSFIFELLDEMIDYG 118
Score = 39.9 bits (89), Expect = 3e-04
Identities = 15/36 (41%), Positives = 25/36 (69%)
Frame = +3
Query: 147 MIGGLFVYNHKGEVLISRVYRDDIGRNAVDAFRVNV 254
MI GLF++N KG+ LI + +R D+ ++ + FRV +
Sbjct: 1 MISGLFIFNLKGDTLICKTFRHDLKKSVTEIFRVAI 36
>SPBC651.11c |apm3||AP-3 adaptor complex subunit Apm3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 425
Score = 35.9 bits (79), Expect = 0.005
Identities = 15/59 (25%), Positives = 32/59 (54%)
Frame = +2
Query: 329 NIWLAAVTKQNVNAAMVFEFLLKIXDVMQSYFGKISEEXXXNNFVLIYELLDEILDFGY 505
++ L T + + + + +I DV++++FG + N +I +LL E++D+GY
Sbjct: 66 DVRLCIPTTCDTEPLYIHDIMRRIVDVVKTFFGGFNASKVEKNVCVIVQLLAEMIDYGY 124
>SPBC685.04c |aps2||AP-2 adaptor complex subunit Aps2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 143
Score = 29.1 bits (62), Expect = 0.63
Identities = 13/56 (23%), Positives = 25/56 (44%)
Frame = +2
Query: 326 ANIWLAAVTKQNVNAAMVFEFLLKIXDVMQSYFGKISEEXXXNNFVLIYELLDEIL 493
A ++ N + E + +++ S+FG + E NF + +LDEI+
Sbjct: 64 AGLYFCFCVDSTDNDLAILEMIHFFVEILDSFFGNVCELDLIFNFYKVSAILDEII 119
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 28.3 bits (60), Expect = 1.1
Identities = 15/54 (27%), Positives = 21/54 (38%)
Frame = -3
Query: 260 HGHIHSECVHSITTNIISVDSRDQHLAFMVINEQAPDHCG*GLTLFQNWFQCNC 99
H H +C + T IS S + IN + P H + NW C+C
Sbjct: 438 HYTCHKKCYPKVVTKCISKSSDSASSEYEKINHRIPHHFESHTNIGANWC-CHC 490
>SPBC3H7.15 |hhp1||serine/threonine protein kinase
Hhp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 365
Score = 27.1 bits (57), Expect = 2.5
Identities = 10/30 (33%), Positives = 16/30 (53%)
Frame = +1
Query: 292 HRTHIFLPYQACKYLAGSGHKAKCERGYGV 381
H+TH+ +PY+ K L G+ A G+
Sbjct: 161 HKTHLHIPYRENKNLTGTARYASINTHLGI 190
>SPBC691.03c |apl3||AP-2 adaptor complex subunit Alp3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 878
Score = 25.8 bits (54), Expect = 5.8
Identities = 16/58 (27%), Positives = 28/58 (48%)
Frame = +3
Query: 63 YVEFLKGCNR*IAITLKPILKECEASPTMIGGLFVYNHKGEVLISRVYRDDIGRNAVD 236
+V ++ G R I + L P+L PT+ +NH G++ + R + G N+ D
Sbjct: 737 HVSYVTGVIRSIDLQL-PVLLSKFMKPTIFDSYDFFNHWGQMGVEREAQLTFGLNSKD 793
>SPCC320.11c ||SPCC330.18|RNA-binding protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 180
Score = 25.4 bits (53), Expect = 7.7
Identities = 10/32 (31%), Positives = 21/32 (65%)
Frame = +2
Query: 407 VMQSYFGKISEEXXXNNFVLIYELLDEILDFG 502
V++++ G+I+++ + V+IY + D L FG
Sbjct: 113 VLKAHVGRITDDTPQHQGVVIYSMNDTPLGFG 144
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,628,474
Number of Sequences: 5004
Number of extensions: 49956
Number of successful extensions: 112
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 108
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 112
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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