Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060132.seq
(686 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putati... 111 6e-25
At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putati... 100 8e-22
At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putati... 100 8e-22
At2g11270.1 68415.m01208 citrate synthase-related contains simil... 33 0.23
At5g36250.1 68418.m04373 protein phosphatase 2C, putative / PP2C... 30 1.3
At1g73430.1 68414.m08500 sec34-like family protein contains Pfam... 30 1.7
At4g15096.1 68417.m02320 hypothetical protein 29 2.2
At5g53030.2 68418.m06586 expressed protein 29 2.9
At5g53030.1 68418.m06587 expressed protein 29 2.9
At2g40955.1 68415.m05057 hypothetical protein 29 2.9
At4g16442.1 68417.m02489 integral membrane family protein contai... 28 5.0
At5g40340.1 68418.m04894 PWWP domain-containing protein KED, Nic... 28 6.7
At4g11560.1 68417.m01853 bromo-adjacent homology (BAH) domain-co... 28 6.7
At3g15750.1 68416.m01995 expressed protein 28 6.7
At2g34990.1 68415.m04293 zinc finger (C3HC4-type RING finger) fa... 28 6.7
At3g51540.1 68416.m05644 expressed protein mucin 5AC, Homo sapie... 27 8.8
At1g05450.1 68414.m00554 protease inhibitor/seed storage/lipid t... 27 8.8
>At3g60100.1 68416.m06711 citrate synthase, mitochondrial, putative
strong similarity to SP|Q43175 Citrate synthase,
mitochondrial precursor {Solanum tuberosum}; contains
Pfam profile PF00285: Citrate synthase
Length = 433
Score = 111 bits (266), Expect = 6e-25
Identities = 62/138 (44%), Positives = 79/138 (57%)
Frame = +1
Query: 256 VGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEVF 435
VG +TVDM+ GGMRG+ GL+WETS+LDADEGIRFRG+SIPECQ+ LP A+ GEEPLPE
Sbjct: 33 VGNITVDMVLGGMRGMTGLLWETSLLDADEGIRFRGMSIPECQKILPSAESGEEPLPESL 92
Query: 436 SGFSSLAIYPPKRK*SAV*RMGGEAELTGLT*XQC*NNMPGKLHPMSQFLRAAVTATSTV 615
P K + +A+ EL + +P HPM+QF A+ V
Sbjct: 93 LWLLLTGKVPTKEQANAL-----STELAHRAAVPAIDALPSTAHPMTQF--ASGVMALQV 145
Query: 616 NLNFXKXYFRRXXTNPKY 669
F K Y + + KY
Sbjct: 146 QSEFQKAYEQGDISKSKY 163
>At2g44350.2 68415.m05517 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 474
Score = 100 bits (240), Expect = 8e-22
Identities = 56/129 (43%), Positives = 74/129 (57%), Gaps = 1/129 (0%)
Frame = +1
Query: 256 VGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEVF 435
+G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPE
Sbjct: 68 LGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGL 127
Query: 436 SGFSSLAIYPPKRK*SAV*R-MGGEAELTGLT*XQC*NNMPGKLHPMSQFLRAAVTATST 612
P K + A+ + + A + + +P HPM+QF A+
Sbjct: 128 LWLLLTGKVPSKEQVEALSKDLANRAAVPDYV-YNAIDALPSTAHPMTQF--ASGVMALQ 184
Query: 613 VNLNFXKXY 639
V F K Y
Sbjct: 185 VQSEFQKAY 193
Score = 34.3 bits (75), Expect = 0.077
Identities = 16/61 (26%), Positives = 35/61 (57%)
Frame = +2
Query: 74 MALFRITSSRLVELQKACPTATVLLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGST 253
++ F SR+ Q + + ++ S+ +LKS LQE IP++Q+++++ + +HG
Sbjct: 7 VSAFTRLRSRVQGQQSSLSNSVRWIQMQSSTDLDLKSQLQELIPEQQDRLKKLKSEHGKV 66
Query: 254 K 256
+
Sbjct: 67 Q 67
>At2g44350.1 68415.m05516 citrate synthase, mitochondrial, putative
strong similarity to SP|P20115 Citrate synthase,
mitochondrial precursor {Arabidopsis thaliana};
contains Pfam profile PF00285: Citrate synthase
Length = 473
Score = 100 bits (240), Expect = 8e-22
Identities = 56/129 (43%), Positives = 74/129 (57%), Gaps = 1/129 (0%)
Frame = +1
Query: 256 VGEVTVDMMYGGMRGIKGLVWETSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPEVF 435
+G +TVDM+ GGMRG+ GL+WETS+LD +EGIRFRGLSIPECQ+ LP A+ G EPLPE
Sbjct: 67 LGNITVDMVIGGMRGMTGLLWETSLLDPEEGIRFRGLSIPECQKVLPTAQSGAEPLPEGL 126
Query: 436 SGFSSLAIYPPKRK*SAV*R-MGGEAELTGLT*XQC*NNMPGKLHPMSQFLRAAVTATST 612
P K + A+ + + A + + +P HPM+QF A+
Sbjct: 127 LWLLLTGKVPSKEQVEALSKDLANRAAVPDYV-YNAIDALPSTAHPMTQF--ASGVMALQ 183
Query: 613 VNLNFXKXY 639
V F K Y
Sbjct: 184 VQSEFQKAY 192
Score = 33.1 bits (72), Expect = 0.18
Identities = 12/33 (36%), Positives = 24/33 (72%)
Frame = +2
Query: 158 SAEQTNLKSILQEKIPKEQEKIREFRKKHGSTK 256
S+ +LKS LQE IP++Q+++++ + +HG +
Sbjct: 34 SSTDLDLKSQLQELIPEQQDRLKKLKSEHGKVQ 66
>At2g11270.1 68415.m01208 citrate synthase-related contains
similarity to Swiss-Prot:P20115 citrate synthase,
mitochondrial precursor [Arabidopsis thaliana]
Length = 83
Score = 32.7 bits (71), Expect = 0.23
Identities = 13/19 (68%), Positives = 16/19 (84%)
Frame = +1
Query: 256 VGEVTVDMMYGGMRGIKGL 312
VG +TVDM+ GGMRG+ GL
Sbjct: 58 VGNITVDMVLGGMRGMTGL 76
>At5g36250.1 68418.m04373 protein phosphatase 2C, putative / PP2C,
putative
Length = 448
Score = 30.3 bits (65), Expect = 1.3
Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
Frame = -1
Query: 365 RPRKRIPSSASSTEVSQTRPLIPRMPPYIISTVTSPT*WNHASFGIRG---SSLVLWESF 195
RP KR P S SS+ + PL+ R+P + ++ T + G +G ++++WE+F
Sbjct: 31 RPPKRRPGSCSSSFDNTEEPLLHRIPGRMFLNGSTDTVSLFSQQGKKGPNQDAMIVWENF 90
>At1g73430.1 68414.m08500 sec34-like family protein contains Pfam
PF04136: Sec34-like family profile; similar to Conserved
oligomeric Golgi complex component 3 (Vesicle docking
protein SEC34 homolog) (p94) (Swiss-Prot:Q96JB2) [Homo
sapiens]
Length = 784
Score = 29.9 bits (64), Expect = 1.7
Identities = 21/74 (28%), Positives = 37/74 (50%), Gaps = 5/74 (6%)
Frame = +3
Query: 408 WRRTTT--RSLFWLLVTGDIPTEAQVKRCLKNGRRG---GANRAHVXPMLKQYARQTASH 572
W R+T+ R+L ++ I + ++++CLK + V PML + TA
Sbjct: 612 WSRSTSLARTLSPRVLESQIDAKKELEKCLKTTCEEFIMSVTKLVVDPMLSFVTKVTAIK 671
Query: 573 VAVSSGCRHRNFNS 614
VA+SSG ++ +S
Sbjct: 672 VALSSGTQNHKVDS 685
>At4g15096.1 68417.m02320 hypothetical protein
Length = 679
Score = 29.5 bits (63), Expect = 2.2
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 392 CPRLRVEKNHYPKSFLASRHWRYTHRSASKALSKEWAAR 508
C +L +++H PK LA + W + +R K L +R
Sbjct: 374 CEQLTCDESHKPKQRLAPKSWMFKYRRDHKILQSPTMSR 412
>At5g53030.2 68418.m06586 expressed protein
Length = 224
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 322 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 429
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At5g53030.1 68418.m06587 expressed protein
Length = 245
Score = 29.1 bits (62), Expect = 2.9
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +1
Query: 322 TSVLDADEGIRFRGLSIPECQQQLPKAKGGEEPLPE 429
T+VLD +R R LS+P + K +G P PE
Sbjct: 98 TTVLDGPYDLRRRSLSLPRSAAVIRKLRGVPAPAPE 133
>At2g40955.1 68415.m05057 hypothetical protein
Length = 679
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/39 (30%), Positives = 20/39 (51%)
Frame = +2
Query: 392 CPRLRVEKNHYPKSFLASRHWRYTHRSASKALSKEWAAR 508
C +L +++H PK LA + W + +R K L +R
Sbjct: 374 CEQLTCDESHKPKQRLAPKSWMFKYRRDHKILHSPTMSR 412
>At4g16442.1 68417.m02489 integral membrane family protein contains
TIGRFAM TIGR01569 : plant integral membrane protein
TIGR01569; contains Pfam PF04535 : Domain of unknown
function (DUF588)
Length = 182
Score = 28.3 bits (60), Expect = 5.0
Identities = 12/38 (31%), Positives = 20/38 (52%)
Frame = +2
Query: 269 QLI*CTVACVESKVWFGKPLCWMPMKESVSVVYPSLSA 382
Q + C V ++ KV F KPL W ++ Y +++A
Sbjct: 71 QSVRCVVGTMKGKVLFSKPLAWAFFSGDQAMAYLNVAA 108
>At5g40340.1 68418.m04894 PWWP domain-containing protein KED,
Nicotiana tabacum, EMBL:AB009883
Length = 1008
Score = 27.9 bits (59), Expect = 6.7
Identities = 19/64 (29%), Positives = 31/64 (48%)
Frame = +2
Query: 191 QEKIPKEQEKIREFRKKHGSTKSERSQLI*CTVACVESKVWFGKPLCWMPMKESVSVVYP 370
+E++P E EK + +KK KS++ + + E V FG P +P KE + +Y
Sbjct: 830 EEEVPNETEKPEKKKKKKREGKSKKKETE-TEFSGAELYVTFG-PGSSLPKKEDLIEIYE 887
Query: 371 SLSA 382
A
Sbjct: 888 KFGA 891
>At4g11560.1 68417.m01853 bromo-adjacent homology (BAH)
domain-containing protein contains Pfam profile PF01426:
BAH domain
Length = 587
Score = 27.9 bits (59), Expect = 6.7
Identities = 10/31 (32%), Positives = 18/31 (58%)
Frame = +3
Query: 399 G*GWRRTTTRSLFWLLVTGDIPTEAQVKRCL 491
G W+ + TR LF+ ++P E+ + RC+
Sbjct: 182 GGNWQSSDTRELFYSFHRDEVPAESVMHRCV 212
>At3g15750.1 68416.m01995 expressed protein
Length = 186
Score = 27.9 bits (59), Expect = 6.7
Identities = 14/36 (38%), Positives = 22/36 (61%)
Frame = +2
Query: 143 LLRGLSAEQTNLKSILQEKIPKEQEKIREFRKKHGS 250
L+RG+S+ L L+EK+ +QE +F+K H S
Sbjct: 79 LVRGVSSALAFLPDELREKLIDKQETREKFQKLHSS 114
>At2g34990.1 68415.m04293 zinc finger (C3HC4-type RING finger)
family protein contains Pfam profile: PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 302
Score = 27.9 bits (59), Expect = 6.7
Identities = 16/37 (43%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Frame = +3
Query: 186 FSRKRFPKN--KRRSANSERSMVPLSRRGHS*YDVRW 290
FS+ R + + RSA SERS+ RR HS D W
Sbjct: 237 FSQARSSRRGYRSRSAGSERSVFSYQRRMHSFSDCAW 273
>At3g51540.1 68416.m05644 expressed protein mucin 5AC, Homo sapiens,
PIR:S53363
Length = 438
Score = 27.5 bits (58), Expect = 8.8
Identities = 19/77 (24%), Positives = 36/77 (46%)
Frame = -1
Query: 347 PSSASSTEVSQTRPLIPRMPPYIISTVTSPT*WNHASFGIRGSSLVLWESFPGEYFLNSS 168
P +S +V++TRP P +I T + + I+ S+ V + PG +S
Sbjct: 259 PKRNTSPDVTRTRPKGRSASPSLIPTFSGTSHTTTTPKSIKPSATVADSTRPGRKLSRAS 318
Query: 167 VQRLDLEVELSQSDMLS 117
VQ ++L+++ +S
Sbjct: 319 VQMAINHLDLARNGKVS 335
>At1g05450.1 68414.m00554 protease inhibitor/seed storage/lipid
transfer protein (LTP)-related similar to geranyl
diphosphate synthase large subunit [Mentha x piperita]
GI:6449052
Length = 50
Score = 27.5 bits (58), Expect = 8.8
Identities = 16/36 (44%), Positives = 19/36 (52%)
Frame = -2
Query: 352 GFLHRHPAQRFPKPDL*FHACHRTSYQL*PLRLSGT 245
G LH HP +R P L + R Y L PLRLS +
Sbjct: 16 GLLHLHPRRRLPLRTL---SSFRIFYLLSPLRLSNS 48
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,676,225
Number of Sequences: 28952
Number of extensions: 298335
Number of successful extensions: 975
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 934
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 972
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1457719448
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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