Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060127.seq
(663 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g29210.1 68415.m03550 splicing factor PWI domain-containing p... 35 0.042
At4g36520.1 68417.m05185 trichohyalin-related low similarity to ... 31 0.68
At2g02720.1 68415.m00214 pectate lyase family protein similar to... 31 0.68
At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containi... 30 1.6
At1g51900.1 68414.m05850 hypothetical protein 29 2.1
At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam d... 29 2.8
At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA methyltran... 29 2.8
At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)... 29 2.8
At3g28770.1 68416.m03591 expressed protein 29 3.6
At1g23230.1 68414.m02906 expressed protein 29 3.6
At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase f... 28 4.8
At3g57780.1 68416.m06436 expressed protein 28 4.8
At3g19840.1 68416.m02513 FF domain-containing protein / WW domai... 28 4.8
At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative si... 28 6.4
At4g05280.1 68417.m00799 Ulp1 protease family protein contains P... 28 6.4
At3g47180.1 68416.m05123 zinc finger (C3HC4-type RING finger) fa... 28 6.4
At3g43390.1 68416.m04592 hypothetical protein similar to At3g243... 28 6.4
>At2g29210.1 68415.m03550 splicing factor PWI domain-containing
protein contains Pfam profile PF01480: PWI domain
Length = 878
Score = 35.1 bits (77), Expect = 0.042
Identities = 33/127 (25%), Positives = 51/127 (40%), Gaps = 4/127 (3%)
Frame = +1
Query: 10 LSARIFYPLPHQKYIDSQWPAP*VTLPP*KT----SPRSPQT*RVSSKASTPAVSVTSTP 177
++ R+ P P + + S PA + PP + SP ++ +P+ S S P
Sbjct: 456 VAQRLPSPPPRRAGLPSPPPAQRLPSPPPRRAGLPSPMRIGGSHAANHLESPSPSSLSPP 515
Query: 178 MRRLCFRLLKTSPLRRPRSLYSTVSRV*FEPAEAHRDSGEEPASGQRCYRSGEGKEQIPE 357
R+ ++L + P+RR RSL RV H G G GK
Sbjct: 516 GRK---KVLPSPPVRRRRSLTPDEERVSLSQGGRHTSPSHIKQDGSMSPVRGRGKSSPSS 572
Query: 358 RHRELRS 378
RH++ RS
Sbjct: 573 RHQKARS 579
>At4g36520.1 68417.m05185 trichohyalin-related low similarity to
SP|Q07283 Trichohyalin {Homo sapiens}
Length = 1400
Score = 31.1 bits (67), Expect = 0.68
Identities = 19/84 (22%), Positives = 36/84 (42%), Gaps = 1/84 (1%)
Frame = +2
Query: 251 REFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD-PTKLKHTETCEKNPLPT 427
+E Q+K + +E+N K+ +E + + K +E + +LK T E+N
Sbjct: 772 QEEKERQIKERQEREENERRAKEVLEQAENERKLKEALEQKENERRLKETREKEENKKKL 831
Query: 428 KDVIEQEKSAXXXXXXXXXANVSR 499
++ IE E+ A + R
Sbjct: 832 REAIELEEKEKRLIEAFERAEIER 855
>At2g02720.1 68415.m00214 pectate lyase family protein similar to
pectate lyase P59 SP:P15722 from [Lycopersicon
esculentum]
Length = 455
Score = 31.1 bits (67), Expect = 0.68
Identities = 17/58 (29%), Positives = 24/58 (41%)
Frame = +2
Query: 275 KHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTKDVIEQE 448
K +E P P+ + + EK+ FLNG K KNP+P K I +
Sbjct: 375 KQVTKREYTPYPEWKSWNWQSEKDYFLNGAYFVQSGKANAWSATPKNPIPRKFAIRPQ 432
>At2g17410.1 68415.m02009 ARID/BRIGHT DNA-binding domain-containing
protein contains Pfam profile PF01388: ARID/BRIGHT DNA
binding domain
Length = 786
Score = 29.9 bits (64), Expect = 1.6
Identities = 19/62 (30%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Frame = +3
Query: 60 TMACSVSDTPSLKDLPKVATDLKSQLEGF---NTSCLRDVDTNEKIVLPSAEDVATEKTQ 230
T S+ + + DLPK+ + SQ E + S +DT E ++ P+AED E
Sbjct: 74 TSLASLEEVTNADDLPKIDDEKNSQFETSPHPSPSPSVALDTEEGLINPTAEDTVEENIV 133
Query: 231 KS 236
S
Sbjct: 134 SS 135
>At1g51900.1 68414.m05850 hypothetical protein
Length = 774
Score = 29.5 bits (63), Expect = 2.1
Identities = 22/76 (28%), Positives = 36/76 (47%)
Frame = +2
Query: 224 DPEVFIRRYREFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTET 403
DP+++IR Y E + K + T + + + D+IE K + L+ I LK +
Sbjct: 362 DPDIYIRSYEESPNEVYKFSLTDLEEEIMENDSIEG--VKCRMLDEIMKKSGHHLKISRP 419
Query: 404 CEKNPLPTKDVIEQEK 451
E P K V E+E+
Sbjct: 420 -EYKPEIEKQVYEEEE 434
>At5g64420.1 68418.m08092 DNA polymerase V family contains Pfam
domain PF04931: DNA polymerase V
Length = 1306
Score = 29.1 bits (62), Expect = 2.8
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +2
Query: 281 TETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 376
TE E + LPD ++ +K K + +N + N+D
Sbjct: 12 TELVENDNLPDSSIVKKKKSKTEKMNTVANYD 43
>At3g02320.1 68416.m00214 N2,N2-dimethylguanosine tRNA
methyltransferase family protein similar to
N2,N2-dimethylguanosine tRNA methyltransferase [Homo
sapiens] GI:11066198; contains Pfam profile PF02005:
N2,N2-dimethylguanosine tRNA methyltransferase
Length = 599
Score = 29.1 bits (62), Expect = 2.8
Identities = 17/49 (34%), Positives = 25/49 (51%)
Frame = +2
Query: 230 EVFIRRYREFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFD 376
E +R +E ++ K T K + +KDA EA KE+ NG+ N D
Sbjct: 45 EFLSKRKQEHEAKSSKRTRPASK--VIEKDASEASKEETPSENGMNNGD 91
>At1g09140.1 68414.m01018 SF2/ASF-like splicing modulator (SRP30)
nearly identical to SF2/ASF-like splicing modulator
Srp30 [Arabidopsis thaliana] GI:4775270
Length = 268
Score = 29.1 bits (62), Expect = 2.8
Identities = 22/71 (30%), Positives = 32/71 (45%), Gaps = 2/71 (2%)
Frame = +1
Query: 103 SPRSPQT*RVSSKASTPAVSVTSTPMRRLCFRLL--KTSPLRRPRSLYSTVSRV*FEPAE 276
SP ++ R S++ P+ S +S R + ++ PL R RSLYS+VSR
Sbjct: 193 SPDDSKSYRSRSRSRGPSCSYSSKSRSVSPARSISPRSRPLSRSRSLYSSVSRSQSRSKS 252
Query: 277 AHRDSGEEPAS 309
R P S
Sbjct: 253 RSRSRSNSPVS 263
>At3g28770.1 68416.m03591 expressed protein
Length = 2081
Score = 28.7 bits (61), Expect = 3.6
Identities = 20/66 (30%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = +2
Query: 239 IRRYREFDSSQLKHTETQEKNPLPD-KDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKN 415
+R E K +E EK + KDA E + NK L+ EN D K + E +++
Sbjct: 768 VRNKEENVQGNKKESEKVEKGEKKESKDAKSVETKDNKKLSSTENRDEAKERSGEDNKED 827
Query: 416 PLPTKD 433
+KD
Sbjct: 828 KEESKD 833
>At1g23230.1 68414.m02906 expressed protein
Length = 1615
Score = 28.7 bits (61), Expect = 3.6
Identities = 14/35 (40%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Frame = -2
Query: 431 PLWGAGSSRTFPCASA*WDRSSRC-RSGICSFPSP 330
PL+G + + P S W+R+ RC R I + PSP
Sbjct: 393 PLYGEDLAMSIPKGSLDWERAVRCIRHAIRTTPSP 427
>At5g60500.1 68418.m07587 undecaprenyl pyrophosphate synthetase
family protein / UPP synthetase family protein contains
putative undecaprenyl diphosphate synthase domain
[PF01255]; similar to S. cerevisiae dehydrodolichyl
diphosphate synthetase (DEDOL-PP
synthase)(Rer2)[SP|P35196], a cis-prenyltransferase
Length = 271
Score = 28.3 bits (60), Expect = 4.8
Identities = 14/40 (35%), Positives = 23/40 (57%)
Frame = -2
Query: 224 LLSGDVFSRRKHNLLIGVDVTETAGVEAFELTLQVCGDLG 105
++ G+ +KHNL IG+D AG + + LQ C ++G
Sbjct: 41 IMDGNRRFAKKHNL-IGLDAGHRAGFISVKYILQYCKEIG 79
>At3g57780.1 68416.m06436 expressed protein
Length = 670
Score = 28.3 bits (60), Expect = 4.8
Identities = 20/51 (39%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +2
Query: 260 DSSQLKHTETQEK-NPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCE 409
DSS+ TE +E N L D E EKE++K L+ I + + ETCE
Sbjct: 72 DSSE--KTEKEETINGLACDDEDEEEKEESKELDAIAHEKTDSVSSPETCE 120
>At3g19840.1 68416.m02513 FF domain-containing protein / WW
domain-containing protein weak similarity to
transcription factor CA150b [Mus musculus] GI:6329166;
contains Pfam profiles PF01846: FF domain, PF00397: WW
domain
Length = 743
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/60 (26%), Positives = 26/60 (43%)
Frame = +1
Query: 289 SGEEPASGQRCYRSGEGKEQIPERHRELRSH*AEAHGNVREEPAPHKGRH*AREISLNHY 468
S E+ A R + + K + ER + SH ++ ++R EP H RE+ Y
Sbjct: 489 SAEQKAQEIRAAAASDFKTMLREREISINSHWSKVKDSLRNEPRYRSVAHEDREVFYYEY 548
>At5g11110.1 68418.m01297 sucrose-phosphate synthase, putative
similar to sucrose-phosphate synthase isoform 1, Citrus
unshiu, PIR:S72648
Length = 894
Score = 27.9 bits (59), Expect = 6.4
Identities = 20/53 (37%), Positives = 29/53 (54%), Gaps = 2/53 (3%)
Frame = +3
Query: 72 SVSDTPS--LKDLPKVATDLKSQLEGFNTSCLRDVDTNEKIVLPSAEDVATEK 224
S SD+PS L+D+ ++ +LK L+G + VDTN AED A E+
Sbjct: 532 SDSDSPSDSLRDINDISLNLKLSLDGEKSGSNNGVDTN-----LDAEDRAAER 579
>At4g05280.1 68417.m00799 Ulp1 protease family protein contains Pfam
profile PF02902: Ulp1 protease family, C-terminal
catalytic domain; similar to At3g24380, At5g36840,
At5g35010, At3g42740, At4g05290, At2g14770, At3g43390,
At2g05560, At4g08880, At1g34730, At1g27790, At1g34740,
At1g27780, At5g36850, At3g42730, At1g52020, At3g24390,
At1g25886, At4g03300
Length = 1312
Score = 27.9 bits (59), Expect = 6.4
Identities = 17/73 (23%), Positives = 31/73 (42%)
Frame = +2
Query: 236 FIRRYREFDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKN 415
+ R ++ QL+ T+ + K P E +K I D K ++ E C
Sbjct: 348 YARVPKQLRPPQLEETDVKRKRNAPGPSPKEPAMKKQ-----ISEMDCDKEENAEDCFGE 402
Query: 416 PLPTKDVIEQEKS 454
P+P + ++E +S
Sbjct: 403 PVPERFIVEMRRS 415
>At3g47180.1 68416.m05123 zinc finger (C3HC4-type RING finger)
family protein contains Pfam domain, PF00097: Zinc
finger, C3HC4 type (RING finger)
Length = 210
Score = 27.9 bits (59), Expect = 6.4
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 1/61 (1%)
Frame = +2
Query: 221 EDPEVFIRRYRE-FDSSQLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHT 397
ED E Y E FDS+ E + L D+++ +E++ FL + DP +L +
Sbjct: 61 EDEEEINENYYEYFDSNGFGVDEDEINEFLEDQESNSNLEEEDDFLEEEDEIDPDQLSYE 120
Query: 398 E 400
E
Sbjct: 121 E 121
>At3g43390.1 68416.m04592 hypothetical protein similar to At3g24380,
At5g36840, At5g35010, At3g42740, At4g05290, At2g14770,
At2g05560, At4g08880, At1g34730, At1g27790, At1g34740,
At1g27780, At5g36850, At3g42730, At1g52020, At3g24390,
At4g05280, At1g25886, At4g03300
Length = 1113
Score = 27.9 bits (59), Expect = 6.4
Identities = 16/62 (25%), Positives = 27/62 (43%)
Frame = +2
Query: 269 QLKHTETQEKNPLPDKDAIEAEKEKNKFLNGIENFDPTKLKHTETCEKNPLPTKDVIEQE 448
QL+ T+ + K P E +K K D K ++ E C P+P + ++E
Sbjct: 413 QLEETDVKRKRNAPGPSPKEPAMKKQK-----SEMDCDKEENAEDCFGEPVPERFIVEMR 467
Query: 449 KS 454
+S
Sbjct: 468 RS 469
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,245,220
Number of Sequences: 28952
Number of extensions: 277241
Number of successful extensions: 885
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 847
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 885
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1393347168
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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