Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060124.seq
(677 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 183 2e-47
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 95 8e-21
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 91 1e-19
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 81 1e-16
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 55 1e-08
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 51 1e-07
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 44 2e-05
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 31 0.12
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.82
SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces p... 29 0.82
SPBC4C3.12 |sep1||fork head transcription factor Sep1|Schizosacc... 28 1.4
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 27 1.9
SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomy... 27 3.3
SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schiz... 26 5.8
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 5.8
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 183 bits (446), Expect = 2e-47
Identities = 81/95 (85%), Positives = 86/95 (90%)
Frame = +2
Query: 242 GKAPHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 421
G+ H G+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELR
Sbjct: 36 GRPRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELR 95
Query: 422 VAPEEHPVLLTEAPLNPKANREKMTQIMFKHSTRP 526
VAPEEHP LLTEAPLNPK+NREKMTQI+F+ P
Sbjct: 96 VAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAP 130
Score = 83.4 bits (197), Expect = 3e-17
Identities = 40/49 (81%), Positives = 42/49 (85%), Gaps = 2/49 (4%)
Frame = +1
Query: 508 QTFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVXHTVPIYR--RIPH 648
+TFN PA YVAIQAVLSLYASGRTTGIVLDSGDGV HTVPIY +PH
Sbjct: 125 ETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPH 173
Score = 81.0 bits (191), Expect = 1e-16
Identities = 35/38 (92%), Positives = 38/38 (100%)
Frame = +3
Query: 141 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 254
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPR
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 39
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 95.1 bits (226), Expect = 8e-21
Identities = 44/104 (42%), Positives = 65/104 (62%), Gaps = 1/104 (0%)
Frame = +2
Query: 242 GKAPHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-EL 418
G+ H+ VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L
Sbjct: 40 GRIKHERVMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQL 99
Query: 419 RVAPEEHPVLLTEAPLNPKANREKMTQIMFKHSTRPPCTSPSKP 550
PEEHP+LLTE PL N+EK+ + ++ P + +P
Sbjct: 100 NTLPEEHPLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQP 143
Score = 60.9 bits (141), Expect = 2e-10
Identities = 26/42 (61%), Positives = 34/42 (80%)
Frame = +1
Query: 508 QTFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVXHTVPIY 633
+T N PA+ ++Q VL+LYAS RTTGIVL+ GDG+ H+VPIY
Sbjct: 130 ETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIY 171
Score = 48.8 bits (111), Expect = 7e-07
Identities = 19/30 (63%), Positives = 24/30 (80%)
Frame = +3
Query: 159 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGR 248
+ +DNGSG KAGFAGDD P+ +FP+ VGR
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGR 41
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 91.5 bits (217), Expect = 1e-19
Identities = 36/76 (47%), Positives = 58/76 (76%)
Frame = +2
Query: 284 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 463
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 464 LNPKANREKMTQIMFK 511
+NP ANREKM + MF+
Sbjct: 110 MNPVANREKMCETMFE 125
Score = 59.3 bits (137), Expect = 5e-10
Identities = 25/38 (65%), Positives = 32/38 (84%)
Frame = +1
Query: 529 MYVAIQAVLSLYASGRTTGIVLDSGDGVXHTVPIYRRI 642
+YVAIQAVLSLYA G ++G+V+DSGDGV H VP+Y +
Sbjct: 132 VYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESV 169
Score = 51.2 bits (117), Expect = 1e-07
Identities = 23/39 (58%), Positives = 29/39 (74%)
Frame = +3
Query: 147 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRIRA 263
E A +V+DNG+G K G+A D+ PR FPSIVGRP +RA
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRA 40
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 81.0 bits (191), Expect = 1e-16
Identities = 35/76 (46%), Positives = 47/76 (61%), Gaps = 1/76 (1%)
Frame = +2
Query: 287 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 463
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 464 LNPKANREKMTQIMFK 511
LNP NRE +IMF+
Sbjct: 133 LNPPENRENTAEIMFE 148
Score = 44.0 bits (99), Expect = 2e-05
Identities = 22/49 (44%), Positives = 32/49 (65%), Gaps = 8/49 (16%)
Frame = +1
Query: 508 QTFNTPAMYVAIQAVLSLYASGRT--------TGIVLDSGDGVXHTVPI 630
++FN +Y+A+QAVL+L AS + TG V+DSGDGV H +P+
Sbjct: 148 ESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPV 196
Score = 40.7 bits (91), Expect = 2e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +3
Query: 159 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 242
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 54.8 bits (126), Expect = 1e-08
Identities = 28/73 (38%), Positives = 40/73 (54%)
Frame = +2
Query: 329 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 508
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 509 KHSTRPPCTSPSK 547
+ S R P T +K
Sbjct: 123 E-SLRCPATYLAK 134
Score = 40.3 bits (90), Expect = 3e-04
Identities = 16/34 (47%), Positives = 24/34 (70%)
Frame = +3
Query: 144 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 245
+EV+A+V+D GS + GF+G+D P+ V PS G
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG 42
Score = 27.1 bits (57), Expect = 2.5
Identities = 11/32 (34%), Positives = 19/32 (59%)
Frame = +1
Query: 508 QTFNTPAMYVAIQAVLSLYASGRTTGIVLDSG 603
++ PA Y+A Q + +ASG+ T ++D G
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIG 154
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 51.2 bits (117), Expect = 1e-07
Identities = 25/85 (29%), Positives = 48/85 (56%), Gaps = 1/85 (1%)
Frame = +2
Query: 275 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 451
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 452 TEAPLNPKANREKMTQIMFKHSTRP 526
TE NP R MT+++F+ P
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAP 145
Score = 36.7 bits (81), Expect = 0.003
Identities = 16/34 (47%), Positives = 23/34 (67%)
Frame = +3
Query: 159 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRIR 260
LV+DNGS +AG+ G+ P+ VF ++V R R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR 60
Score = 29.9 bits (64), Expect = 0.35
Identities = 12/43 (27%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +1
Query: 508 QTFNTPAMYVAIQAVLSLYASGR--TTGIVLDSGDGVXHTVPI 630
+ +N P++ I + S Y + + ++GIVL+ G+ H +P+
Sbjct: 140 ELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPV 182
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 44.4 bits (100), Expect = 2e-05
Identities = 23/88 (26%), Positives = 46/88 (52%), Gaps = 2/88 (2%)
Frame = +2
Query: 254 HQGVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 427
+ GV + ++ YV DE Q I + +K +GI+ +W+ W +L+V
Sbjct: 39 YYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVN 98
Query: 428 PEEHPVLLTEAPLNPKANREKMTQIMFK 511
P E+ +++TE NP++ R+++ + F+
Sbjct: 99 PTEYAMMITEPSWNPQSVRQQIMEAAFE 126
Score = 36.7 bits (81), Expect = 0.003
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +3
Query: 144 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 245
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
Score = 30.3 bits (65), Expect = 0.27
Identities = 13/54 (24%), Positives = 27/54 (50%)
Frame = +1
Query: 469 PQGQQREDDPDHVQTFNTPAMYVAIQAVLSLYASGRTTGIVLDSGDGVXHTVPI 630
PQ +++ + + PA Y+ QAV +A+ ++T +++D G P+
Sbjct: 113 PQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIGSDNASVTPV 166
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 31.5 bits (68), Expect = 0.12
Identities = 21/73 (28%), Positives = 32/73 (43%), Gaps = 4/73 (5%)
Frame = +2
Query: 344 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFK 511
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ F+
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 512 HSTRPPCTSPSKP 550
P T +P
Sbjct: 172 ECQVPGFTIAYEP 184
Score = 25.4 bits (53), Expect = 7.6
Identities = 12/36 (33%), Positives = 18/36 (50%)
Frame = +1
Query: 523 PAMYVAIQAVLSLYASGRTTGIVLDSGDGVXHTVPI 630
P +A + ++ LYA G G+V+D G PI
Sbjct: 176 PGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPI 211
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 28.7 bits (61), Expect = 0.82
Identities = 14/43 (32%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = +2
Query: 404 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFKHSTRP 526
+Y L E+HP+LLT+ A L P+ + ++ +I K P
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPAC13F5.04c |||endosomal sorting protein |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 277
Score = 28.7 bits (61), Expect = 0.82
Identities = 18/66 (27%), Positives = 28/66 (42%)
Frame = -2
Query: 376 IPVSDDSVFDGVFEGQDTSFALCLISYIRVLLSHTDHHALMRGLPTIEGNTARGASSPAK 197
+P + +V +TSFAL S L A + P +E + +S+PA
Sbjct: 63 LPTQEAAVETNASASHETSFALPTTSPAASLSISPTKSAAVSSEPNVEADVKSLSSTPAA 122
Query: 196 PALHIP 179
P L+ P
Sbjct: 123 PQLNSP 128
>SPBC4C3.12 |sep1||fork head transcription factor
Sep1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 663
Score = 27.9 bits (59), Expect = 1.4
Identities = 24/74 (32%), Positives = 33/74 (44%)
Frame = +2
Query: 407 YNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFKHSTRPPCTSPSKPCSRCTRPVVPPV 586
Y+EL +A +E P L P PK K+ ++ S KP S+ TRP +P
Sbjct: 23 YSELPIAYQEIP--LQSLPPYPKV-ASKLKGVV-AGGKENNIASFQKPSSKATRPYIP-- 76
Query: 587 SCWTPATVSXTPCP 628
+T T S P P
Sbjct: 77 -SYTRLTYSVPPLP 89
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 27.5 bits (58), Expect = 1.9
Identities = 11/23 (47%), Positives = 14/23 (60%)
Frame = -1
Query: 311 VPHLLHKSPSVPYRPSRPDAGPS 243
+ L+ K+PS PY SRP A S
Sbjct: 306 IDQLISKAPSYPYSSSRPSASAS 328
>SPAC2F3.13c |||queuine tRNA-ribosyltransferase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 348
Score = 26.6 bits (56), Expect = 3.3
Identities = 11/31 (35%), Positives = 18/31 (58%)
Frame = +1
Query: 463 PQPQGQQREDDPDHVQTFNTPAMYVAIQAVL 555
P +G PD+V+ F+ PA+YV ++ L
Sbjct: 32 PTSRGTVPHLTPDNVEEFDIPALYVGLEDCL 62
>SPAC637.07 |moe1||translation initiation factor eIF3d
Moe1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 567
Score = 25.8 bits (54), Expect = 5.8
Identities = 12/27 (44%), Positives = 12/27 (44%)
Frame = -3
Query: 645 RYPSVDGHGVXDTVAGVQHDTGGTTGR 565
RY S G G DTV GG GR
Sbjct: 133 RYGSSGGRGAGDTVVSRSSGAGGARGR 159
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 25.8 bits (54), Expect = 5.8
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -2
Query: 340 FEGQDTSFALCLISYIRVLLSHTDHHA 260
+EG+DT+ +Y+R++L TD A
Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,957,687
Number of Sequences: 5004
Number of extensions: 64176
Number of successful extensions: 203
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 200
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 311890690
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -