Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060123.seq
(692 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putati... 116 1e-26
At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2... 115 3e-26
At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1... 114 5e-26
At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1... 114 5e-26
At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putati... 109 1e-24
At1g04540.1 68414.m00446 C2 domain-containing protein low simila... 29 2.9
At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase... 29 3.9
At2g23390.1 68415.m02793 expressed protein 29 3.9
At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplas... 27 8.9
At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplas... 27 8.9
At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplas... 27 8.9
>At3g17390.1 68416.m02222 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 2 (Methionine
adenosyltransferase 2, AdoMet synthetase 2)
[Catharanthus roseus] SWISS-PROT:Q96552
Length = 393
Score = 116 bits (279), Expect = 1e-26
Identities = 66/150 (44%), Positives = 85/150 (56%), Gaps = 2/150 (1%)
Frame = +1
Query: 244 KSCIETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQ 423
K ET TKT MV++ GEIT+KANVDY+++VR+T + IG+ + G D C V++ ++Q
Sbjct: 39 KVACETCTKTNMVMVFGEITTKANVDYEQIVRKTCREIGFVSADVGLDADNCKVLVNIEQ 98
Query: 424 QSPNIAAGVHEN--RNDEEVGAGDQGLMFGLQQMRQKNACR*L*CLHTNSIRKLQSFRRN 597
QSP+IA GVH + + EEVGAGDQG MFG L T KL R+N
Sbjct: 99 QSPDIAQGVHGHLTKKPEEVGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158
Query: 598 GRFWWARPDSKNTGLLANMYLPGGATVPHR 687
G W RPD K T + GA VP R
Sbjct: 159 GTCPWLRPDGK-TQVTIEYINESGAMVPVR 187
Score = 72.1 bits (169), Expect = 3e-13
Identities = 38/70 (54%), Positives = 47/70 (67%), Gaps = 4/70 (5%)
Frame = +2
Query: 140 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVALKP*LKP----VWCFCVAKSHP 307
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVA + K V+ K++
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVACETCTKTNMVMVFGEITTKANV 63
Query: 308 KLTWIIKKLC 337
I++K C
Sbjct: 64 DYEQIVRKTC 73
>At4g01850.1 68417.m00242 S-adenosylmethionine synthetase 2 (SAM2)
identical to S-adenosylmethionine synthetase 2
(Methionine adenosyltransferase 2, AdoMet synthetase 2)
[Arabidopsis thaliana] SWISS-PROT:P17562
Length = 393
Score = 115 bits (277), Expect = 3e-26
Identities = 64/156 (41%), Positives = 85/156 (54%), Gaps = 2/156 (1%)
Frame = +1
Query: 226 ESGSGRKSCIETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSV 405
E K ET TKT MV++ GEIT+KA +DY+K+VR+T + IG+ G D C V
Sbjct: 33 EQDPDSKVACETCTKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKV 92
Query: 406 MLALDQQSPNIAAGVHEN--RNDEEVGAGDQGLMFGLQQMRQKNACR*L*CLHTNSIRKL 579
++ ++QQSP+IA GVH + + E++GAGDQG MFG L T +L
Sbjct: 93 LVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYATDETPELMPLSHVLATKIGARL 152
Query: 580 QSFRRNGRFWWARPDSKNTGLLANMYLPGGATVPHR 687
R+NG W RPD K T + Y GA VP R
Sbjct: 153 TEVRKNGTCRWLRPDGK-TQVTVEYYNDNGAMVPVR 187
Score = 72.9 bits (171), Expect = 2e-13
Identities = 32/38 (84%), Positives = 35/38 (92%)
Frame = +2
Query: 140 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVA 253
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVA
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41
>At1g02500.2 68414.m00201 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 114 bits (275), Expect = 5e-26
Identities = 66/156 (42%), Positives = 85/156 (54%), Gaps = 2/156 (1%)
Frame = +1
Query: 226 ESGSGRKSCIETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSV 405
E K ET TKT MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V
Sbjct: 33 EQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKV 92
Query: 406 MLALDQQSPNIAAGVHEN--RNDEEVGAGDQGLMFGLQQMRQKNACR*L*CLHTNSIRKL 579
++ ++QQSP+IA GVH + + EE+GAGDQG MFG L T +L
Sbjct: 93 LVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARL 152
Query: 580 QSFRRNGRFWWARPDSKNTGLLANMYLPGGATVPHR 687
R+NG W RPD K T + Y GA VP R
Sbjct: 153 TEVRKNGTCAWLRPDGK-TQVTVEYYNDKGAMVPIR 187
Score = 72.9 bits (171), Expect = 2e-13
Identities = 32/38 (84%), Positives = 35/38 (92%)
Frame = +2
Query: 140 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVA 253
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVA
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41
>At1g02500.1 68414.m00200 S-adenosylmethionine synthetase 1 (SAM1)
identical to S-adenosylmethionine synthetase 1
(Methionine adenosyltransferase 1, AdoMet synthetase 1)
[Arabidopsis thaliana] SWISS-PROT:P23686
Length = 393
Score = 114 bits (275), Expect = 5e-26
Identities = 66/156 (42%), Positives = 85/156 (54%), Gaps = 2/156 (1%)
Frame = +1
Query: 226 ESGSGRKSCIETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSV 405
E K ET TKT MV++ GEIT+KA VDY+K+VR+T + IG+ G D C V
Sbjct: 33 EQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRAIGFVSDDVGLDADKCKV 92
Query: 406 MLALDQQSPNIAAGVHEN--RNDEEVGAGDQGLMFGLQQMRQKNACR*L*CLHTNSIRKL 579
++ ++QQSP+IA GVH + + EE+GAGDQG MFG L T +L
Sbjct: 93 LVNIEQQSPDIAQGVHGHFTKCPEEIGAGDQGHMFGYATDETPELMPLSHVLATKLGARL 152
Query: 580 QSFRRNGRFWWARPDSKNTGLLANMYLPGGATVPHR 687
R+NG W RPD K T + Y GA VP R
Sbjct: 153 TEVRKNGTCAWLRPDGK-TQVTVEYYNDKGAMVPIR 187
Score = 72.9 bits (171), Expect = 2e-13
Identities = 32/38 (84%), Positives = 35/38 (92%)
Frame = +2
Query: 140 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVA 253
FLFTSESV EGHPDK+CDQISDA+LDA L QDPD+KVA
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVA 41
>At2g36880.1 68415.m04521 S-adenosylmethionine synthetase, putative
similar to S-adenosylmethionine synthetase 3 (Methionine
adenosyltransferase 3, AdoMet synthetase 3)
[Lycopersicon esculentum] SWISS-PROT:P43282
Length = 390
Score = 109 bits (263), Expect = 1e-24
Identities = 62/150 (41%), Positives = 83/150 (55%), Gaps = 2/150 (1%)
Frame = +1
Query: 244 KSCIETITKTGMVLLCGEITSKANVDYQKVVRETVKHIGYDDSSKGFDYKTCSVMLALDQ 423
K ET TKT MV++ GEIT+ A VDY+K+VR T + IG+ + G D C+V++ ++Q
Sbjct: 39 KVACETCTKTNMVMVFGEITTAAKVDYEKIVRSTCREIGFISADVGLDADKCNVLVNIEQ 98
Query: 424 QSPNIAAGVHEN--RNDEEVGAGDQGLMFGLQQMRQKNACR*L*CLHTNSIRKLQSFRRN 597
QSP+IA GVH + + E++GAGDQG MFG L T KL R+N
Sbjct: 99 QSPDIAQGVHGHLTKKPEDIGAGDQGHMFGYATDETPELMPLTHVLATKLGAKLTEVRKN 158
Query: 598 GRFWWARPDSKNTGLLANMYLPGGATVPHR 687
W RPD K T + GGA +P R
Sbjct: 159 KTCPWLRPDGK-TQVTVEYKNDGGAMIPIR 187
Score = 71.7 bits (168), Expect = 4e-13
Identities = 32/38 (84%), Positives = 35/38 (92%)
Frame = +2
Query: 140 FLFTSESVGEGHPDKMCDQISDAILDAHLNQDPDAKVA 253
FLFTSESV EGHPDK+CDQISDAILDA L QDP++KVA
Sbjct: 4 FLFTSESVNEGHPDKLCDQISDAILDACLEQDPESKVA 41
>At1g04540.1 68414.m00446 C2 domain-containing protein low
similarity to cold-regulated gene SRC2 [Glycine max]
GI:2055230; contains Pfam profile PF00168: C2 domain
Length = 601
Score = 29.1 bits (62), Expect = 2.9
Identities = 12/42 (28%), Positives = 21/42 (50%)
Frame = +1
Query: 169 GSSRQNVRPNKRRYSRRASESGSGRKSCIETITKTGMVLLCG 294
G S + P ++++RR ++ G+G SC I +CG
Sbjct: 528 GMSSRRKTPTTKKHNRRHTDGGNGLFSCFSKICGVECSFVCG 569
>At5g40440.1 68418.m04904 mitogen-activated protein kinase kinase
(MAPKK), putative (MKK3) similar to NPK2 [Nicotiana
tabacum] gi|862342|dbj|BAA06731; mitogen-activated
protein kinase kinase (MAPKK) family, PMID:12119167
Length = 520
Score = 28.7 bits (61), Expect = 3.9
Identities = 18/42 (42%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = -3
Query: 612 PPKSPISPEALQFSD*VCVQA---LQSTACILLSHLLQTEHQ 496
PPK SPE F D C+Q + TA LLSH T+H+
Sbjct: 303 PPKQEFSPEFCSFID-ACLQKDPDARPTADQLLSHPFITKHE 343
>At2g23390.1 68415.m02793 expressed protein
Length = 469
Score = 28.7 bits (61), Expect = 3.9
Identities = 17/61 (27%), Positives = 27/61 (44%)
Frame = +3
Query: 477 WGRRPGLDVRFATDETEECMPLTVVLAHKLNQKIAELQAKWEILVGKTRFKKHRFTCEYV 656
WG RP D + + E C + A +LN K E A+ E + + ++C Y+
Sbjct: 374 WGCRP--DSYYPSLHFEACYYQAIEAAIELNLKTVEAGAQGEHKIQRGYLPVKTYSCHYI 431
Query: 657 F 659
F
Sbjct: 432 F 432
>At5g58330.3 68418.m07302 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 334
Score = 27.5 bits (58), Expect = 8.9
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +2
Query: 68 PETSKMNGXAKTNGHSYDMEDGSVFLFTSESVGEGHPDKMCDQISDAILDAHLNQ 232
PE + T+G+ Y +E+G VF S G+G + + D +D +L Q
Sbjct: 244 PEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGD----YELVKDVEIDDYLRQ 294
>At5g58330.2 68418.m07304 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 442
Score = 27.5 bits (58), Expect = 8.9
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +2
Query: 68 PETSKMNGXAKTNGHSYDMEDGSVFLFTSESVGEGHPDKMCDQISDAILDAHLNQ 232
PE + T+G+ Y +E+G VF S G+G + + D +D +L Q
Sbjct: 352 PEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGD----YELVKDVEIDDYLRQ 402
>At5g58330.1 68418.m07303 malate dehydrogenase [NADP], chloroplast,
putative strong similiarity to chloroplast
NADP-dependent malate dehydrogenase (EC 1.1.1.82)
SP|O48902 {Medicago sativa}, SP|P21528 {Pisum sativum},
SP|Q05145 {Mesembryanthemum crystallinum}, SP|P46489
{Flaveria bidentis}, [Flaveria trinervia] GI:726334,
SP|P17606I {Sorghum bicolor}; contains InterPro entry
IPR001236: Lactate/malate dehydrogenase
Length = 443
Score = 27.5 bits (58), Expect = 8.9
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +2
Query: 68 PETSKMNGXAKTNGHSYDMEDGSVFLFTSESVGEGHPDKMCDQISDAILDAHLNQ 232
PE + T+G+ Y +E+G VF S G+G + + D +D +L Q
Sbjct: 353 PEGDWFSTGVYTDGNPYGIEEGLVFSMPCRSKGDGD----YELVKDVEIDDYLRQ 403
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 16,760,552
Number of Sequences: 28952
Number of extensions: 376482
Number of successful extensions: 1030
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 984
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1015
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1477286152
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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