BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060120.seq (679 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g76130.1 68414.m08841 alpha-amylase, putative / 1,4-alpha-D-g... 28 4.9 >At1g76130.1 68414.m08841 alpha-amylase, putative / 1,4-alpha-D-glucan glucanohydrolase, putative strong similarity to alpha-amylase GI:7532799 from [Malus x domestica];contains Pfam profile PF00128: Alpha amylase, catalytic domain Length = 413 Score = 28.3 bits (60), Expect = 4.9 Identities = 15/30 (50%), Positives = 19/30 (63%), Gaps = 2/30 (6%) Frame = +1 Query: 421 YLPQ--FIKNFEFNA*HLLFSLLHKMKHYQ 504 YLPQ + N + + HLL SLL KMK Y+ Sbjct: 74 YLPQDLYSLNSAYGSEHLLKSLLRKMKQYK 103 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,368,586 Number of Sequences: 28952 Number of extensions: 205611 Number of successful extensions: 300 Number of sequences better than 10.0: 1 Number of HSP's better than 10.0 without gapping: 297 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 300 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1428369392 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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