Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060117.seq
(559 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces pomb... 111 9e-26
SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces... 70 3e-13
SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyc... 67 2e-12
SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyce... 62 7e-11
SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1... 31 0.11
SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor |Schizo... 27 2.5
SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit L2|Sc... 26 4.3
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 26 4.3
SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pomb... 25 5.7
SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces po... 25 7.5
SPAC31A2.12 |||arrestin/PY protein 1|Schizosaccharomyces pombe|c... 25 10.0
>SPAC1002.03c |gls2||glucosidase II Gls2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 923
Score = 111 bits (266), Expect = 9e-26
Identities = 54/118 (45%), Positives = 67/118 (56%)
Frame = +1
Query: 205 VLVTGNSREQFLRF*RXGFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDG 384
VL G S F GFF P+AEL RWY+ A F PFFRAH+HI+TKRREPWL +
Sbjct: 615 VLTNGISGMAFSGADVAGFFGNPDAELFVRWYETAIFYPFFRAHAHIDTKRREPWLYGEP 674
Query: 385 TTVLIRDANRKRYALLDXWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLGD 558
T L+R+ R RY LL WYT Y G + P F + + + IDDQ+ +GD
Sbjct: 675 YTSLVRELLRIRYRLLPTWYTAFYNSHTHGFPILYPQFLMHPEDEEGFAIDDQFYVGD 732
Score = 87.8 bits (208), Expect = 9e-19
Identities = 38/83 (45%), Positives = 50/83 (60%)
Frame = +2
Query: 5 WEHRHVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSF 184
WEHR +HN YG + T GL+ R +G RPF+LTR+ FAGT +A W GD W
Sbjct: 548 WEHRDIHNIYGHKCINGTYNGLIKRGEGAVRPFILTRSFFAGTSALAANWIGDTMTTWEH 607
Query: 185 LAASVPMCLSLAIAGNSFCGSDV 253
L S+P L+ I+G +F G+DV
Sbjct: 608 LRGSIPTVLTNGISGMAFSGADV 630
Score = 24.6 bits (51), Expect = 10.0
Identities = 9/20 (45%), Positives = 12/20 (60%)
Frame = +3
Query: 96 DPSCSRGPXSQAPRDTLRYG 155
+PS RGP + RD + YG
Sbjct: 527 EPSVFRGPETSMHRDAIHYG 546
>SPAC1039.11c ||SPAC922.02c|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 995
Score = 69.7 bits (163), Expect = 3e-13
Identities = 36/100 (36%), Positives = 53/100 (53%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRKRYALLD 435
GF + EL +RW AF PF+R H+ + + +EP+ + R A RY+LL
Sbjct: 701 GFMGNTDEELCSRWMALGAFLPFYRNHNSLGSISQEPY-RWESVAESSRCAMNIRYSLLP 759
Query: 436 XWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLG 555
WYTL YE + GL +RPLF + + P D Q+++G
Sbjct: 760 YWYTLMYEASSQGLPLIRPLFFEFPNEPSLANADRQFMVG 799
Score = 56.4 bits (130), Expect = 3e-09
Identities = 29/79 (36%), Positives = 43/79 (54%)
Frame = +2
Query: 17 HVHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSFLAAS 196
++ N YG R T L V RPF+L+R+ F G+ +Y+A W GDN + WS + S
Sbjct: 622 NLFNTYGYDQSRVTYDSLTSIEPNV-RPFILSRSTFVGSGKYAAHWLGDNYSLWSNMIFS 680
Query: 197 VPMCLSLAIAGNSFCGSDV 253
+P L+ + G G+DV
Sbjct: 681 IPGALTFNMVGLPMVGADV 699
>SPAPB24D3.10c |agl1|agl|alpha-glucosidase Agl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 969
Score = 66.9 bits (156), Expect = 2e-12
Identities = 34/101 (33%), Positives = 52/101 (51%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRKRYALLD 435
GF + EL +RW AF PF+R H++I +EP+ R A RY+LL
Sbjct: 679 GFLGDSDEELCSRWMAMGAFSPFYRNHNNIYQISQEPYTW-SSVAEASRRAMYIRYSLLP 737
Query: 436 XWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLGD 558
WYT+ + + DG +R LF + + P +D Q+++GD
Sbjct: 738 YWYTIMAKASQDGTPALRALFVEFPNDPTLADVDRQFMVGD 778
Score = 44.4 bits (100), Expect = 1e-05
Identities = 24/78 (30%), Positives = 38/78 (48%)
Frame = +2
Query: 20 VHNEYGLWNLRATNTGLLDRADGVYRPFLLTRAXFAGTQRYSAVWTGDNTAEWSFLAASV 199
+ N YG + + L + RPF+L+R+ F G+ Y A W GDN + WS + S+
Sbjct: 601 IFNMYGYGETKVSYAALTQISPNE-RPFILSRSTFLGSGVYGAHWLGDNHSLWSNMFFSI 659
Query: 200 PMCLSLAIAGNSFCGSDV 253
+ + G G+DV
Sbjct: 660 SGMIVFNMMGIPMVGADV 677
>SPAC30D11.01c ||SPAC56F8.01|alpha-glucosidase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 993
Score = 61.7 bits (143), Expect = 7e-11
Identities = 33/100 (33%), Positives = 50/100 (50%)
Frame = +1
Query: 256 GFFKYPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRKRYALLD 435
G+ + EL RW AF PF+R H+ + + +EP+ R A RY+LL
Sbjct: 704 GYNGNTDEELCARWMALGAFLPFYRNHNSLGSIPQEPFRWAS-VAEASRSAIEIRYSLLP 762
Query: 436 XWYTLXYEHTVDGLTXMRPLFQXYLDXPVTYXIDDQYLLG 555
WYTL + +VDG +RPLF + +D Q+++G
Sbjct: 763 YWYTLMHTASVDGTPMVRPLFFEFPKQISLASVDKQFMIG 802
Score = 55.6 bits (128), Expect = 5e-09
Identities = 23/53 (43%), Positives = 35/53 (66%)
Frame = +2
Query: 95 RPFLLTRAXFAGTQRYSAVWTGDNTAEWSFLAASVPMCLSLAIAGNSFCGSDV 253
RPFLL+R+ F G+ RY+A W GDN ++WS + +S+ L+ + G G+DV
Sbjct: 650 RPFLLSRSTFVGSGRYAAHWLGDNKSQWSDMVSSISSILTFNLLGIPMVGADV 702
>SPAC110.01 |ppk1|SPAC140.05|serine/threonine protein kinase Ppk1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1023
Score = 31.1 bits (67), Expect = 0.11
Identities = 21/81 (25%), Positives = 35/81 (43%)
Frame = -2
Query: 267 LEESSTSEPQKLFPAIASDKHIGTEAARNDHSAVLSPVHTAEYLWVPANXALVSRKGL*T 88
L + +P P +++ + + + H LSP Y +P++ + SR G T
Sbjct: 58 LSGNENPQPSSESPYFTNNERLNSSSFPQIHDNQLSPSFNTSYQAIPSSSSNRSRGGPYT 117
Query: 87 PSARSSSPVLVARRFHSPYSL 25
PS R S +L F S + L
Sbjct: 118 PSIRDDS-LLALLSFSSNHRL 137
>SPCC417.05c |chr2|cfh2|chitin synthase regulatory factor
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 512
Score = 26.6 bits (56), Expect = 2.5
Identities = 14/50 (28%), Positives = 25/50 (50%)
Frame = +1
Query: 268 YPEAELMTRWYQAAAFQPFFRAHSHIETKRREPWLXXDGTTVLIRDANRK 417
YP EL W +AAA++ A + ++ +E + T++ I + RK
Sbjct: 434 YPNPELANEWAKAAAYKNHHLASTFVQDVGKED--VFEKTSITISNEERK 481
>SPCC16C4.15 |rml2||mitochondrial ribosomal protein subunit
L2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 318
Score = 25.8 bits (54), Expect = 4.3
Identities = 11/30 (36%), Positives = 19/30 (63%)
Frame = -2
Query: 270 ILEESSTSEPQKLFPAIASDKHIGTEAARN 181
+L+ S EP ++ P +A++ GT AA+N
Sbjct: 132 LLKTGSNGEPVEMDPVLAAEIEDGTFAAKN 161
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 25.8 bits (54), Expect = 4.3
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1
Query: 412 RKRYALLDXWYTLXYEHTVDGLTXM 486
++RYA+L WY L E+ D T M
Sbjct: 83 KERYAILRRWYDLIMENADDLATMM 107
>SPCC1840.03 |sal3|pse1|karyopherin Sal3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1095
Score = 25.4 bits (53), Expect = 5.7
Identities = 9/29 (31%), Positives = 15/29 (51%)
Frame = -2
Query: 456 VXQSVPXVEQRVSLAVGVPNEHGGAVXVQ 370
+ S P + Q S +GV +HGG + +
Sbjct: 918 IQSSEPEIRQAASYGIGVAAQHGGELYAE 946
>SPAC343.05 |vma1||V-type ATPase subunit A|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 619
Score = 25.0 bits (52), Expect = 7.5
Identities = 12/29 (41%), Positives = 15/29 (51%)
Frame = -1
Query: 151 YRRVSLGACEXGPREQEGSVDAVGAVQQP 65
Y R C P ++EG+V VGAV P
Sbjct: 394 YERAGRARCLGSP-DREGTVSIVGAVSPP 421
>SPAC31A2.12 |||arrestin/PY protein 1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 596
Score = 24.6 bits (51), Expect = 10.0
Identities = 17/53 (32%), Positives = 20/53 (37%)
Frame = +3
Query: 249 TXRILQVSRS*AHDEVVPGGRVPAVLSGPLAHRDQAARALAXPGRHHRAHSGR 407
T + L RS H G P+ S LAH QA+ HR S R
Sbjct: 519 TRQSLDSRRSFEHSTSSSSGISPSHSSASLAHLSQASNPNGSSSAPHRPTSAR 571
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,050,625
Number of Sequences: 5004
Number of extensions: 37711
Number of successful extensions: 131
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 233995432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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