BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060115.seq (695 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosac... 95 9e-21 SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elon... 95 9e-21 SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyce... 46 7e-06 SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Ma... 46 7e-06 SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Sc... 27 1.9 SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 26 4.5 SPAC1A6.01c ||SPAC23C4.20c|human thyroid receptor interacting pr... 26 5.9 >SPCP31B10.07 |eft202||translation elongation factor 2 |Schizosaccharomyces pombe|chr 3|||Manual Length = 842 Score = 95.1 bits (226), Expect = 9e-21 Identities = 59/151 (39%), Positives = 76/151 (50%), Gaps = 7/151 (4%) Frame = +1 Query: 253 QFKIDLVKLMNRLWGENFFNPXTKKWSKQKDD----DNKRSFCMYVLDPIYXXFDAIMKF 420 +F ID K+M RLWGEN+FNP TKKWSK D N+R+F M++LDPIY FDA+M Sbjct: 232 KFGIDRNKMMQRLWGENYFNPKTKKWSKSATDANGNSNQRAFNMFILDPIYRIFDAVMNS 291 Query: 421 KKEEXDDLLXKIGVTIKHXDXXKDGKXXXXXXXXXXXXXVKXCFX*LPFIYHHL*WP--- 591 +K+E LL K+ VTIK + +GK + I HL P Sbjct: 292 RKDEVFTLLSKLEVTIKPDEKELEGKALLKVVMRKFLPAADAL---MEMIVLHLPSPKTA 348 Query: 592 SKYRMEMLXRXXPIDG*SCPLGXXXCXXEGP 684 +YR E L P+D C +G C P Sbjct: 349 QQYRAETLYE-GPMDD-ECAVGIRNCDANAP 377 Score = 85.0 bits (201), Expect = 9e-18 Identities = 39/81 (48%), Positives = 53/81 (65%) Frame = +2 Query: 8 RPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDDGGPMGXVRVDPSKGSV 187 RP++ +NK+DR YQ F R+VE+VNV+I+TY D +G +V P KG+V Sbjct: 152 RPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDK--VLGDCQVFPDKGTV 209 Query: 188 GFGSGLHGWAFTLKQFSEMYA 250 F SGLHGWAFT++QF+ YA Sbjct: 210 AFASGLHGWAFTVRQFANRYA 230 >SPAC513.01c |eft201|eft2-1, etf2, SPAPYUK71.04c|translation elongation factor 2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 842 Score = 95.1 bits (226), Expect = 9e-21 Identities = 59/151 (39%), Positives = 76/151 (50%), Gaps = 7/151 (4%) Frame = +1 Query: 253 QFKIDLVKLMNRLWGENFFNPXTKKWSKQKDD----DNKRSFCMYVLDPIYXXFDAIMKF 420 +F ID K+M RLWGEN+FNP TKKWSK D N+R+F M++LDPIY FDA+M Sbjct: 232 KFGIDRNKMMQRLWGENYFNPKTKKWSKSATDANGNSNQRAFNMFILDPIYRIFDAVMNS 291 Query: 421 KKEEXDDLLXKIGVTIKHXDXXKDGKXXXXXXXXXXXXXVKXCFX*LPFIYHHL*WP--- 591 +K+E LL K+ VTIK + +GK + I HL P Sbjct: 292 RKDEVFTLLSKLEVTIKPDEKELEGKALLKVVMRKFLPAADAL---MEMIVLHLPSPKTA 348 Query: 592 SKYRMEMLXRXXPIDG*SCPLGXXXCXXEGP 684 +YR E L P+D C +G C P Sbjct: 349 QQYRAETLYE-GPMDD-ECAVGIRNCDANAP 377 Score = 85.0 bits (201), Expect = 9e-18 Identities = 39/81 (48%), Positives = 53/81 (65%) Frame = +2 Query: 8 RPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDDGGPMGXVRVDPSKGSV 187 RP++ +NK+DR YQ F R+VE+VNV+I+TY D +G +V P KG+V Sbjct: 152 RPVVVVNKVDRALLELQISQEELYQNFARVVESVNVVISTYYDK--VLGDCQVFPDKGTV 209 Query: 188 GFGSGLHGWAFTLKQFSEMYA 250 F SGLHGWAFT++QF+ YA Sbjct: 210 AFASGLHGWAFTVRQFANRYA 230 >SPBC215.12 |cwf10|spef2, snu114|GTPase Cwf10 |Schizosaccharomyces pombe|chr 2|||Manual Length = 983 Score = 45.6 bits (103), Expect = 7e-06 Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 1/80 (1%) Frame = +1 Query: 262 IDLVKLMNRLWGENFFNPXTKKWSKQK-DDDNKRSFCMYVLDPIYXXFDAIMKFKKEEXD 438 ID+ RLWG+ +F+ T+K++KQ D RSF ++L+P+Y + + E+ Sbjct: 345 IDVDLFSKRLWGDIYFDSKTRKFAKQSLDGSGVRSFVHFILEPLYKLHTLTISDEAEKLK 404 Query: 439 DLLXKIGVTIKHXDXXKDGK 498 L + +K D D K Sbjct: 405 KHLSSFQIYLKPKDYLLDPK 424 Score = 39.5 bits (88), Expect = 4e-04 Identities = 24/80 (30%), Positives = 36/80 (45%) Frame = +2 Query: 14 ILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDDGGPMGXVRVDPSKGSVGF 193 +L +NK+DR Y + +++ VN I D RV P G+V F Sbjct: 265 VLVLNKVDRLILELRLPPNDAYHKLRHVIDEVNDNICQISKDL----KYRVSPELGNVCF 320 Query: 194 GSGLHGWAFTLKQFSEMYAD 253 S G+ FTL F+++Y D Sbjct: 321 ASCDLGYCFTLSSFAKLYID 340 >SPCC553.08c |||GTPase Ria1 |Schizosaccharomyces pombe|chr 3|||Manual Length = 1000 Score = 45.6 bits (103), Expect = 7e-06 Identities = 30/88 (34%), Positives = 39/88 (44%), Gaps = 10/88 (11%) Frame = +2 Query: 14 ILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYX----------DDGGPMGXVR 163 IL +NKMDR + R+VE VN +I T+ D+ + Sbjct: 150 ILVINKMDRLITELKLSPIEAHYHLLRLVEQVNAVIGTFYTGELMQLADNDEVISDEGIY 209 Query: 164 VDPSKGSVGFGSGLHGWAFTLKQFSEMY 247 P +G+V F S GWAF L QFSE Y Sbjct: 210 FAPEQGNVVFASAYDGWAFCLDQFSEFY 237 Score = 27.1 bits (57), Expect = 2.6 Identities = 14/68 (20%), Positives = 33/68 (48%), Gaps = 4/68 (5%) Frame = +1 Query: 289 LWGENFFNPXTKKWSKQKDDDNKR---SFCMYVLDPIYXXFD-AIMKFKKEEXDDLLXKI 456 LWG+ + +P TK+ + K +R F +VL+ ++ ++ A+ E + ++ + Sbjct: 252 LWGDYYLDPKTKRVLQPKHLQGRRLKPMFVQFVLENLWAVYESAVSNRNLENIEKIIKAL 311 Query: 457 GVTIKHXD 480 + + D Sbjct: 312 NIKVLPRD 319 Score = 26.2 bits (55), Expect = 4.5 Identities = 10/27 (37%), Positives = 15/27 (55%) Frame = +3 Query: 513 VMRSWLPXGEALLQXIAIHLPSPVVAQ 593 + + WLP A+L +PSP+ AQ Sbjct: 332 IFQQWLPLSTAILLTAIREIPSPINAQ 358 >SPBC106.09 |cut4|apc1|anaphase-promoting complex subunit Apc1|Schizosaccharomyces pombe|chr 2|||Manual Length = 1458 Score = 27.5 bits (58), Expect = 1.9 Identities = 11/26 (42%), Positives = 17/26 (65%) Frame = +1 Query: 271 VKLMNRLWGENFFNPXTKKWSKQKDD 348 ++L+NRL ++F +P T KW K D Sbjct: 639 LRLVNRLDVDSFLHPKTPKWVFNKQD 664 >SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 2685 Score = 26.2 bits (55), Expect = 4.5 Identities = 19/74 (25%), Positives = 29/74 (39%) Frame = -2 Query: 229 FEGESPPMKTRPETNRALARVDXXXXXXXXXIVICGYNDVNIFYNTLERLV*FFSFKLEL 50 FE S M+ RPETN + + VIC N+ Y + + L+L Sbjct: 170 FELGSLSMEMRPETNNVVGYENPSDPELVSSCVICSIRLANLVYIDRRQSSQQITDYLDL 229 Query: 49 KKSTVHFVHEQNRP 8 T + + + N P Sbjct: 230 SLQTYYSLDKSNVP 243 >SPAC1A6.01c ||SPAC23C4.20c|human thyroid receptor interacting protein homolog|Schizosaccharomyces pombe|chr 1|||Manual Length = 455 Score = 25.8 bits (54), Expect = 5.9 Identities = 10/22 (45%), Positives = 13/22 (59%) Frame = +1 Query: 298 ENFFNPXTKKWSKQKDDDNKRS 363 E+ N +KWSK +DDD S Sbjct: 432 ESILNKINQKWSKVQDDDGMPS 453 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,507,105 Number of Sequences: 5004 Number of extensions: 45189 Number of successful extensions: 98 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 82 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 90 length of database: 2,362,478 effective HSP length: 71 effective length of database: 2,007,194 effective search space used: 321151040 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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