Bombyx BLAST Result against arabidopsis

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060115.seq
         (695 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2, p...    92   4e-19
At1g06220.2 68414.m00656 elongation factor Tu family protein sim...    50   1e-06
At1g06220.1 68414.m00655 elongation factor Tu family protein sim...    50   1e-06
At5g25230.1 68418.m02991 elongation factor Tu family protein tra...    47   1e-05
At3g22980.1 68416.m02898 elongation factor Tu family protein sim...    34   0.078
At1g18485.1 68414.m02307 pentatricopeptide (PPR) repeat-containi...    31   0.73 
At2g27035.1 68415.m03248 plastocyanin-like domain-containing pro...    29   2.9  
At1g75800.1 68414.m08805 pathogenesis-related thaumatin family p...    29   2.9  

>At1g56070.1 68414.m06438 elongation factor 2, putative / EF-2,
           putative similar to ELONGATION FACTOR 2 GB:O14460 from
           [Schizosaccharomyces pombe]
          Length = 843

 Score = 91.9 bits (218), Expect = 4e-19
 Identities = 50/112 (44%), Positives = 65/112 (58%), Gaps = 2/112 (1%)
 Frame = +2

Query: 8   RPILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDDGGPMGXVRVDPSKGSV 187
           RP+L +NKMDR            YQTF R++EN NVI+ATY D    +G V+V P KG+V
Sbjct: 152 RPVLTVNKMDRCFLELQVDGEEAYQTFSRVIENANVIMATYEDP--LLGDVQVYPEKGTV 209

Query: 188 GFGSGLHGWAFTLKQFSEMYADNSRLTLSSL*TG-YGXKTFSTLXRR-SGXN 337
            F +GLHGWAFTL  F++MYA    +  S +    +G   F    R+ SG N
Sbjct: 210 AFSAGLHGWAFTLTNFAKMYASKFGVVESKMMERLWGENFFDPATRKWSGKN 261



 Score = 60.1 bits (139), Expect = 1e-09
 Identities = 29/77 (37%), Positives = 44/77 (57%), Gaps = 2/77 (2%)
 Frame = +1

Query: 274 KLMNRLWGENFFNPXTKKWSKQKDDDN--KRSFCMYVLDPIYXXFDAIMKFKKEEXDDLL 447
           K+M RLWGENFF+P T+KWS +       KR F  +  +PI       M  +K++   +L
Sbjct: 239 KMMERLWGENFFDPATRKWSGKNTGSPTCKRGFVQFCYEPIKQIIATCMNDQKDKLWPML 298

Query: 448 XKIGVTIKHXDXXKDGK 498
            K+GV++K+ +    GK
Sbjct: 299 AKLGVSMKNDEKELMGK 315



 Score = 36.7 bits (81), Expect = 0.015
 Identities = 15/28 (53%), Positives = 20/28 (71%)
 Frame = +3

Query: 513 VMRSWLPXGEALLQXIAIHLPSPVVAQQ 596
           VM++WLP   ALL+ +  HLPSP  AQ+
Sbjct: 321 VMQTWLPASTALLEMMIFHLPSPHTAQR 348


>At1g06220.2 68414.m00656 elongation factor Tu family protein
           similar to Cryptosporidium parvum elongation factor-2
           GB:U21667 GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 25/79 (31%), Positives = 41/79 (51%)
 Frame = +2

Query: 14  ILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDDGGPMGXVRVDPSKGSVGF 193
           ++ +NK+DR            Y   +  +E +N  I+      G +  +  DP+ G+V F
Sbjct: 265 VVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAASTTAGDLPLI--DPAAGNVCF 322

Query: 194 GSGLHGWAFTLKQFSEMYA 250
            SG  GW+FTL+ F++MYA
Sbjct: 323 ASGTAGWSFTLQSFAKMYA 341



 Score = 44.4 bits (100), Expect = 7e-05
 Identities = 17/72 (23%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
 Frame = +1

Query: 262 IDLVKLMNRLWGENFFNPXTKKWSKQKD-DDNKRSFCMYVLDPIYXXFDAIMKFKKEEXD 438
           +D+ K  +RLWG+ +++  T+ + +       +R+F  ++L+P+Y  +  ++   K+  +
Sbjct: 348 MDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVE 407

Query: 439 DLLXKIGVTIKH 474
             L ++GVT+ +
Sbjct: 408 TTLAELGVTLSN 419


>At1g06220.1 68414.m00655 elongation factor Tu family protein
           similar to Cryptosporidium parvum elongation factor-2
           GB:U21667 GI:706974 from [Cryptosporidium parvum]
          Length = 987

 Score = 50.0 bits (114), Expect = 1e-06
 Identities = 25/79 (31%), Positives = 41/79 (51%)
 Frame = +2

Query: 14  ILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDDGGPMGXVRVDPSKGSVGF 193
           ++ +NK+DR            Y   +  +E +N  I+      G +  +  DP+ G+V F
Sbjct: 265 VVVINKVDRLITELKLPPRDAYYKLRHTIEVINNHISAASTTAGDLPLI--DPAAGNVCF 322

Query: 194 GSGLHGWAFTLKQFSEMYA 250
            SG  GW+FTL+ F++MYA
Sbjct: 323 ASGTAGWSFTLQSFAKMYA 341



 Score = 44.4 bits (100), Expect = 7e-05
 Identities = 17/72 (23%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
 Frame = +1

Query: 262 IDLVKLMNRLWGENFFNPXTKKWSKQKD-DDNKRSFCMYVLDPIYXXFDAIMKFKKEEXD 438
           +D+ K  +RLWG+ +++  T+ + +       +R+F  ++L+P+Y  +  ++   K+  +
Sbjct: 348 MDVDKFASRLWGDVYYHSDTRVFKRSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVE 407

Query: 439 DLLXKIGVTIKH 474
             L ++GVT+ +
Sbjct: 408 TTLAELGVTLSN 419


>At5g25230.1 68418.m02991 elongation factor Tu family protein
           translation Elongation Factor 2, Schizosaccharomyces
           pombe, PIR:T39902
          Length = 973

 Score = 47.2 bits (107), Expect = 1e-05
 Identities = 24/79 (30%), Positives = 40/79 (50%)
 Frame = +2

Query: 14  ILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDDGGPMGXVRVDPSKGSVGF 193
           ++ +NK+DR            Y   +  +E +N  I+    +   +  +  DP+ G+V F
Sbjct: 251 VVVINKVDRLITELKLPPRDAYYKLRYTIEVINNHISAASTNAADLPLI--DPAAGNVCF 308

Query: 194 GSGLHGWAFTLKQFSEMYA 250
            SG  GW+FTL+ F+ MYA
Sbjct: 309 ASGTAGWSFTLQSFARMYA 327



 Score = 46.8 bits (106), Expect = 1e-05
 Identities = 18/72 (25%), Positives = 43/72 (59%), Gaps = 1/72 (1%)
 Frame = +1

Query: 262 IDLVKLMNRLWGENFFNPXTKKWSKQKD-DDNKRSFCMYVLDPIYXXFDAIMKFKKEEXD 438
           +D+ K  +RLWG+ +++P T+ ++        +R+F  ++L+P+Y  +  ++   K+  +
Sbjct: 334 MDVDKFASRLWGDVYYHPDTRVFNTSPPVGGGERAFVQFILEPLYKIYSQVIGEHKKSVE 393

Query: 439 DLLXKIGVTIKH 474
             L ++GVT+ +
Sbjct: 394 TTLAELGVTLSN 405


>At3g22980.1 68416.m02898 elongation factor Tu family protein
           similar to eukaryotic translation elongation factor 2
           GB:NP_001952 [Homo sapiens]
          Length = 1015

 Score = 34.3 bits (75), Expect = 0.078
 Identities = 29/114 (25%), Positives = 43/114 (37%), Gaps = 24/114 (21%)
 Frame = +2

Query: 11  PILFMNKMDRXXXXXXXXXXXXYQTFQRIVENVNVIIATYXDD----------GGPMGXV 160
           P L +NK+DR            Y    RIV  VN I++ Y  +            P G +
Sbjct: 129 PCLVLNKIDRLIFELRLSPMEAYTRLIRIVHEVNGIVSAYKSEKYLSDVDSILASPSGEL 188

Query: 161 RVD--------------PSKGSVGFGSGLHGWAFTLKQFSEMYADNSRLTLSSL 280
             +              P KG+V F   L GW F + +F+  YA     + ++L
Sbjct: 189 SAESLELLEDDEEVTFQPQKGNVVFVCALDGWGFGIAEFANFYASKLGASATAL 242



 Score = 33.1 bits (72), Expect = 0.18
 Identities = 13/27 (48%), Positives = 16/27 (59%)
 Frame = +3

Query: 513 VMRSWLPXGEALLQXIAIHLPSPVVAQ 593
           VM  WLP  +A+L     HLP P+ AQ
Sbjct: 328 VMSRWLPLSDAVLSMAVKHLPDPIAAQ 354


>At1g18485.1 68414.m02307 pentatricopeptide (PPR) repeat-containing
           protein contains INTERPRO:IPR002885 PPR repeats
          Length = 970

 Score = 31.1 bits (67), Expect = 0.73
 Identities = 16/58 (27%), Positives = 30/58 (51%)
 Frame = +2

Query: 185 VGFGSGLHGWAFTLKQFSEMYADNSRLTLSSL*TGYGXKTFSTLXRRSGXNKRMMTTN 358
           +G G G+HGWA  L+   E+  +N+ + + S     G  T + +  +   NK +++ N
Sbjct: 308 IGLGKGVHGWAVKLRLDKELVLNNALMDMYS---KCGCITNAQMIFKMNNNKNVVSWN 362


>At2g27035.1 68415.m03248 plastocyanin-like domain-containing
           protein low similarity to SP:P80728 Mavicyanin
           {Cucurbita pepo}; contains Pfam profile PF02298:
           Plastocyanin-like domain
          Length = 163

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 11/36 (30%), Positives = 20/36 (55%)
 Frame = -3

Query: 201 PDPKPTEPLLGSTRTSPMGPPSSXYVAIMTLTFSTI 94
           P P P+ P   ++ T+P+ PP++   AI+   F  +
Sbjct: 126 PQPPPSAPTNFTSTTTPLIPPNAITAAILIFAFKAL 161


>At1g75800.1 68414.m08805 pathogenesis-related thaumatin family
           protein similar to receptor serine/threonine kinase PR5K
           [Arabidopsis thaliana] GI:1235680; contains Pfam
           profile: PF00314 Thaumatin family
          Length = 330

 Score = 29.1 bits (62), Expect = 2.9
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = -3

Query: 201 PDPKPTEPLLGSTRTSPMGPPSSXYVAIMT 112
           PDPKPT P  G++ T+P G  S+ +  + T
Sbjct: 262 PDPKPTTP-TGTSSTTPAGDSSTTWSPVDT 290


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,470,271
Number of Sequences: 28952
Number of extensions: 250961
Number of successful extensions: 572
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 538
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 570
length of database: 12,070,560
effective HSP length: 79
effective length of database: 9,783,352
effective search space used: 1487069504
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)