BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060114.seq (644 letters) Database: uniref50 1,657,284 sequences; 575,637,011 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value UniRef50_Q6AW71 Cluster: RNA-dependent RNA polymerase; n=1; Bomb... 95 2e-18 UniRef50_P35928 Cluster: RNA replicase polyprotein; n=2; Erysimu... 46 8e-04 UniRef50_Q9IW08 Cluster: Replicase-associated protein; n=10; Tym... 46 0.001 UniRef50_Q0IKR9 Cluster: Polyprotein; n=8; Tymoviridae|Rep: Poly... 45 0.002 UniRef50_P89920 Cluster: Replicase-associated polyprotein; n=5; ... 44 0.003 UniRef50_Q91TW9 Cluster: Polyprotein; n=25; Marafivirus|Rep: Pol... 40 0.051 UniRef50_P20126 Cluster: RNA replicase polyprotein; n=3; Tymovir... 40 0.068 UniRef50_P10358 Cluster: RNA replicase polyprotein; n=8; Tymovir... 38 0.16 UniRef50_Q3HWZ1 Cluster: Polyprotein; n=7; Citrus sudden death-a... 36 0.63 UniRef50_Q32WC7 Cluster: Replicase; n=1; Dulcamara mottle virus|... 36 0.63 UniRef50_A6PS51 Cluster: Putative uncharacterized protein precur... 33 4.5 UniRef50_Q2UBZ7 Cluster: Predicted protein; n=1; Aspergillus ory... 33 7.8 >UniRef50_Q6AW71 Cluster: RNA-dependent RNA polymerase; n=1; Bombyx mori Macula-like latent virus|Rep: RNA-dependent RNA polymerase - Bombyx mori Macula-like latent virus Length = 1747 Score = 94.7 bits (225), Expect = 2e-18 Identities = 44/45 (97%), Positives = 45/45 (100%) Frame = +1 Query: 328 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSKLM 462 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSKL+ Sbjct: 3 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSKLI 47 Score = 87.0 bits (206), Expect = 3e-16 Identities = 40/41 (97%), Positives = 41/41 (100%) Frame = +3 Query: 510 MAFTNLVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPYQ 632 MAFTNLVDTLANTIHRDAITAPLV+TAISNFRHKLQLYPYQ Sbjct: 1 MAFTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQ 41 Score = 85.0 bits (201), Expect = 1e-15 Identities = 39/41 (95%), Positives = 41/41 (100%) Frame = +2 Query: 134 MAFTNLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPYR 256 MAFTNLV+TLANTIHRDAITAPLVETAISNFRHKLQLYPY+ Sbjct: 1 MAFTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQ 41 Score = 66.5 bits (155), Expect = 5e-10 Identities = 33/38 (86%), Positives = 34/38 (89%) Frame = +1 Query: 10 RDAITAPLVETAISNFRH*LQLYPYQVNSKLM*NLNPL 123 RDAITAPLVETAISNFRH LQLYPYQVNSKL+ LN L Sbjct: 16 RDAITAPLVETAISNFRHKLQLYPYQVNSKLIPLLNQL 53 >UniRef50_P35928 Cluster: RNA replicase polyprotein; n=2; Erysimum latent virus|Rep: RNA replicase polyprotein - Erysimum latent virus (ELV) Length = 1748 Score = 46.0 bits (104), Expect = 8e-04 Identities = 18/45 (40%), Positives = 33/45 (73%) Frame = +1 Query: 328 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSKLM 462 F +D L++T HRD+I+APL+++++S + L+L+PY V +L+ Sbjct: 3 FQLALDALSSTTHRDSISAPLLDSSVSQLQSSLELFPYTVPKELV 47 Score = 45.6 bits (103), Expect = 0.001 Identities = 18/40 (45%), Positives = 30/40 (75%) Frame = +3 Query: 510 MAFTNLVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 MAF +D L++T HRD+I+APL+ +++S + L+L+PY Sbjct: 1 MAFQLALDALSSTTHRDSISAPLLDSSVSQLQSSLELFPY 40 Score = 44.8 bits (101), Expect = 0.002 Identities = 17/40 (42%), Positives = 31/40 (77%) Frame = +2 Query: 134 MAFTNLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPY 253 MAF ++ L++T HRD+I+APL+++++S + L+L+PY Sbjct: 1 MAFQLALDALSSTTHRDSISAPLLDSSVSQLQSSLELFPY 40 Score = 37.9 bits (84), Expect = 0.21 Identities = 16/38 (42%), Positives = 28/38 (73%) Frame = +1 Query: 4 TSRDAITAPLVETAISNFRH*LQLYPYQVNSKLM*NLN 117 T RD+I+APL+++++S + L+L+PY V +L+ LN Sbjct: 14 THRDSISAPLLDSSVSQLQSSLELFPYTVPKELVPQLN 51 >UniRef50_Q9IW08 Cluster: Replicase-associated protein; n=10; Tymoviridae|Rep: Replicase-associated protein - Poinsettia mosaic virus Length = 1987 Score = 45.6 bits (103), Expect = 0.001 Identities = 18/40 (45%), Positives = 27/40 (67%) Frame = +2 Query: 134 MAFTNLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPY 253 MAF + N L++T+HRD + APL+E+ R L+LYP+ Sbjct: 1 MAFQDAFNNLSSTVHRDTVAAPLLESIAQPLRDSLELYPW 40 Score = 44.0 bits (99), Expect = 0.003 Identities = 16/43 (37%), Positives = 29/43 (67%) Frame = +1 Query: 328 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSK 456 F + + L++T+HRD + APL+E+ R L+LYP+ +N++ Sbjct: 3 FQDAFNNLSSTVHRDTVAAPLLESIAQPLRDSLELYPWAINAE 45 Score = 41.9 bits (94), Expect = 0.013 Identities = 16/40 (40%), Positives = 27/40 (67%) Frame = +3 Query: 510 MAFTNLVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 MAF + + L++T+HRD + APL+++ R L+LYP+ Sbjct: 1 MAFQDAFNNLSSTVHRDTVAAPLLESIAQPLRDSLELYPW 40 >UniRef50_Q0IKR9 Cluster: Polyprotein; n=8; Tymoviridae|Rep: Polyprotein - Grapevine rupestris vein feathering virus Length = 2068 Score = 44.8 bits (101), Expect = 0.002 Identities = 19/37 (51%), Positives = 24/37 (64%) Frame = +1 Query: 337 LVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQV 447 LV+ L T+HRD + +PLVE A R LQLYPY + Sbjct: 137 LVEILNPTVHRDTVCSPLVEAAAGPLRDSLQLYPYDI 173 Score = 44.0 bits (99), Expect = 0.003 Identities = 21/45 (46%), Positives = 24/45 (53%) Frame = +2 Query: 119 PYSSSMAFTNLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPY 253 P S LV L T+HRD + +PLVE A R LQLYPY Sbjct: 127 PALSGGGLKELVEILNPTVHRDTVCSPLVEAAAGPLRDSLQLYPY 171 Score = 41.9 bits (94), Expect = 0.013 Identities = 18/35 (51%), Positives = 23/35 (65%) Frame = +3 Query: 525 LVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 LV+ L T+HRD + +PLV+ A R LQLYPY Sbjct: 137 LVEILNPTVHRDTVCSPLVEAAAGPLRDSLQLYPY 171 Score = 33.1 bits (72), Expect = 5.9 Identities = 14/27 (51%), Positives = 17/27 (62%) Frame = +1 Query: 10 RDAITAPLVETAISNFRH*LQLYPYQV 90 RD + +PLVE A R LQLYPY + Sbjct: 147 RDTVCSPLVEAAAGPLRDSLQLYPYDI 173 >UniRef50_P89920 Cluster: Replicase-associated polyprotein; n=5; Oat blue dwarf virus|Rep: Replicase-associated polyprotein - Oat blue dwarf virus Length = 2066 Score = 44.0 bits (99), Expect = 0.003 Identities = 18/38 (47%), Positives = 29/38 (76%) Frame = +1 Query: 334 NLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQV 447 ++++TL++TIHRD I APL+ET S +R L+ +P+ V Sbjct: 26 DVIETLSSTIHRDTIAAPLMETLASPYRDSLRDFPWAV 63 Score = 43.2 bits (97), Expect = 0.006 Identities = 19/42 (45%), Positives = 31/42 (73%) Frame = +2 Query: 146 NLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPYR*IPSS 271 +++ TL++TIHRD I APL+ET S +R L+ +P+ +P+S Sbjct: 26 DVIETLSSTIHRDTIAAPLMETLASPYRDSLRDFPWA-VPAS 66 Score = 41.1 bits (92), Expect = 0.022 Identities = 16/36 (44%), Positives = 28/36 (77%) Frame = +3 Query: 522 NLVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 ++++TL++TIHRD I APL++T S +R L+ +P+ Sbjct: 26 DVIETLSSTIHRDTIAAPLMETLASPYRDSLRDFPW 61 >UniRef50_Q91TW9 Cluster: Polyprotein; n=25; Marafivirus|Rep: Polyprotein - Maize rayado fino virus Length = 2027 Score = 39.9 bits (89), Expect = 0.051 Identities = 16/36 (44%), Positives = 24/36 (66%) Frame = +1 Query: 340 VDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQV 447 V++L T HRD ITAP+VE+ + R L+ YP+ + Sbjct: 14 VESLTPTTHRDTITAPIVESLATPLRRSLERYPWSI 49 Score = 39.1 bits (87), Expect = 0.090 Identities = 19/42 (45%), Positives = 25/42 (59%) Frame = +2 Query: 152 VNTLANTIHRDAITAPLVETAISNFRHKLQLYPYR*IPSS*H 277 V +L T HRD ITAP+VE+ + R L+ YP+ IP H Sbjct: 14 VESLTPTTHRDTITAPIVESLATPLRRSLERYPWS-IPKEFH 54 Score = 37.1 bits (82), Expect = 0.36 Identities = 15/34 (44%), Positives = 23/34 (67%) Frame = +3 Query: 528 VDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 V++L T HRD ITAP+V++ + R L+ YP+ Sbjct: 14 VESLTPTTHRDTITAPIVESLATPLRRSLERYPW 47 >UniRef50_P20126 Cluster: RNA replicase polyprotein; n=3; Tymovirus|Rep: RNA replicase polyprotein - Eggplant mosaic virus Length = 1839 Score = 39.5 bits (88), Expect = 0.068 Identities = 16/40 (40%), Positives = 25/40 (62%) Frame = +3 Query: 510 MAFTNLVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 MAF + ++ L +T HRDA T P++ + + R L LYP+ Sbjct: 1 MAFQSALEALNSTTHRDASTNPILNSVVEPLRDSLSLYPW 40 Score = 38.7 bits (86), Expect = 0.12 Identities = 16/40 (40%), Positives = 24/40 (60%) Frame = +2 Query: 134 MAFTNLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPY 253 MAF + + L +T HRDA T P++ + + R L LYP+ Sbjct: 1 MAFQSALEALNSTTHRDASTNPILNSVVEPLRDSLSLYPW 40 Score = 35.9 bits (79), Expect = 0.84 Identities = 14/38 (36%), Positives = 23/38 (60%) Frame = +1 Query: 328 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPY 441 F + ++ L +T HRDA T P++ + + R L LYP+ Sbjct: 3 FQSALEALNSTTHRDASTNPILNSVVEPLRDSLSLYPW 40 >UniRef50_P10358 Cluster: RNA replicase polyprotein; n=8; Tymovirus|Rep: RNA replicase polyprotein - Turnip yellow mosaic virus Length = 1844 Score = 38.3 bits (85), Expect = 0.16 Identities = 15/45 (33%), Positives = 26/45 (57%) Frame = +1 Query: 328 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSKLM 462 F +D LA T HRD P++E+ + + R +Q YP+ + +L+ Sbjct: 3 FQLALDALAPTTHRDPSLHPILESTVDSIRSSIQTYPWSIPKELL 47 Score = 37.1 bits (82), Expect = 0.36 Identities = 15/40 (37%), Positives = 24/40 (60%) Frame = +3 Query: 510 MAFTNLVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 MAF +D LA T HRD P++++ + + R +Q YP+ Sbjct: 1 MAFQLALDALAPTTHRDPSLHPILESTVDSIRSSIQTYPW 40 Score = 36.7 bits (81), Expect = 0.48 Identities = 15/40 (37%), Positives = 24/40 (60%) Frame = +2 Query: 134 MAFTNLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPY 253 MAF ++ LA T HRD P++E+ + + R +Q YP+ Sbjct: 1 MAFQLALDALAPTTHRDPSLHPILESTVDSIRSSIQTYPW 40 >UniRef50_Q3HWZ1 Cluster: Polyprotein; n=7; Citrus sudden death-associated virus|Rep: Polyprotein - Citrus sudden death-associated virus Length = 2189 Score = 36.3 bits (80), Expect = 0.63 Identities = 19/48 (39%), Positives = 27/48 (56%) Frame = +2 Query: 158 TLANTIHRDAITAPLVETAISNFRHKLQLYPYR*IPSS*HKQPTKSAC 301 +LA T HRD I PL+E +R L YP+ IP+ + QP ++C Sbjct: 97 SLAPTTHRDTIATPLMEALAEPYRQSLSTYPWH-IPT--NLQPFLTSC 141 Score = 36.3 bits (80), Expect = 0.63 Identities = 15/38 (39%), Positives = 21/38 (55%) Frame = +1 Query: 346 TLANTIHRDAITAPLVETAISNFRHKLQLYPYQVNSKL 459 +LA T HRD I PL+E +R L YP+ + + L Sbjct: 97 SLAPTTHRDTIATPLMEALAEPYRQSLSTYPWHIPTNL 134 >UniRef50_Q32WC7 Cluster: Replicase; n=1; Dulcamara mottle virus|Rep: Replicase - Dulcamara mottle virus Length = 1742 Score = 36.3 bits (80), Expect = 0.63 Identities = 13/40 (32%), Positives = 26/40 (65%) Frame = +3 Query: 510 MAFTNLVDTLANTIHRDAITAPLVKTAISNFRHKLQLYPY 629 MAF + ++ L +T HRDA++ P++ + + + L+ YP+ Sbjct: 1 MAFQSALEALNSTTHRDAVSHPILTSVVRPLQDSLETYPW 40 Score = 35.5 bits (78), Expect = 1.1 Identities = 13/40 (32%), Positives = 25/40 (62%) Frame = +2 Query: 134 MAFTNLVNTLANTIHRDAITAPLVETAISNFRHKLQLYPY 253 MAF + + L +T HRDA++ P++ + + + L+ YP+ Sbjct: 1 MAFQSALEALNSTTHRDAVSHPILTSVVRPLQDSLETYPW 40 Score = 32.7 bits (71), Expect = 7.8 Identities = 11/38 (28%), Positives = 24/38 (63%) Frame = +1 Query: 328 FTNLVDTLANTIHRDAITAPLVETAISNFRHKLQLYPY 441 F + ++ L +T HRDA++ P++ + + + L+ YP+ Sbjct: 3 FQSALEALNSTTHRDAVSHPILTSVVRPLQDSLETYPW 40 >UniRef50_A6PS51 Cluster: Putative uncharacterized protein precursor; n=1; Victivallis vadensis ATCC BAA-548|Rep: Putative uncharacterized protein precursor - Victivallis vadensis ATCC BAA-548 Length = 581 Score = 33.5 bits (73), Expect = 4.5 Identities = 9/16 (56%), Positives = 13/16 (81%) Frame = -3 Query: 591 WRFSLGEPLWRLGGWC 544 W F+LG P+W++ GWC Sbjct: 30 WLFALGVPVWKMAGWC 45 Score = 32.7 bits (71), Expect = 7.8 Identities = 9/16 (56%), Positives = 12/16 (75%) Frame = -2 Query: 403 WRFPLGEPLWRLGGWC 356 W F LG P+W++ GWC Sbjct: 30 WLFALGVPVWKMAGWC 45 Score = 32.7 bits (71), Expect = 7.8 Identities = 9/16 (56%), Positives = 12/16 (75%) Frame = -1 Query: 215 WRFPLGEPLWRLGGWC 168 W F LG P+W++ GWC Sbjct: 30 WLFALGVPVWKMAGWC 45 >UniRef50_Q2UBZ7 Cluster: Predicted protein; n=1; Aspergillus oryzae|Rep: Predicted protein - Aspergillus oryzae Length = 110 Score = 32.7 bits (71), Expect = 7.8 Identities = 12/28 (42%), Positives = 18/28 (64%) Frame = -3 Query: 546 CWRECRLNW*KPCKRRSQGQADFVGCLC 463 CWR+ ++ W P +R+ +G F GCLC Sbjct: 84 CWRDSQIRWFLPDRRQLRGNCYF-GCLC 110 Database: uniref50 Posted date: Oct 5, 2007 11:19 AM Number of letters in database: 575,637,011 Number of sequences in database: 1,657,284 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 592,664,015 Number of Sequences: 1657284 Number of extensions: 10711128 Number of successful extensions: 24376 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 16607 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 24372 length of database: 575,637,011 effective HSP length: 97 effective length of database: 414,880,463 effective search space used: 48541014171 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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