BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060113.seq (656 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g11620.2 68416.m01420 expressed protein similar to unknown pr... 28 4.8 At3g11620.1 68416.m01419 expressed protein similar to unknown pr... 28 4.8 At1g13120.1 68414.m01521 expressed protein contains Prosite PS00... 28 4.8 At2g39930.1 68415.m04907 isoamylase, putative / starch debranchi... 28 6.3 At1g61030.1 68414.m06871 expressed protein 28 6.3 >At3g11620.2 68416.m01420 expressed protein similar to unknown protein GB:AAF00672 (Arabidopsis thaliana) Length = 312 Score = 28.3 bits (60), Expect = 4.8 Identities = 14/32 (43%), Positives = 15/32 (46%) Frame = +1 Query: 19 LFITQKKTIHHTKVFIERYNRVHSFCLTVLKS 114 LF K HT + IER H FC TV S Sbjct: 262 LFEEISKQAPHTSLSIEREGHTHGFCCTVAGS 293 >At3g11620.1 68416.m01419 expressed protein similar to unknown protein GB:AAF00672 (Arabidopsis thaliana) Length = 312 Score = 28.3 bits (60), Expect = 4.8 Identities = 14/32 (43%), Positives = 15/32 (46%) Frame = +1 Query: 19 LFITQKKTIHHTKVFIERYNRVHSFCLTVLKS 114 LF K HT + IER H FC TV S Sbjct: 262 LFEEISKQAPHTSLSIEREGHTHGFCCTVAGS 293 >At1g13120.1 68414.m01521 expressed protein contains Prosite PS00012: Phosphopantetheine attachment site; similar to GLE1 (GI:3288817) {Homo sapiens}; EST gb|N37870 comes from this gene Length = 611 Score = 28.3 bits (60), Expect = 4.8 Identities = 18/48 (37%), Positives = 27/48 (56%), Gaps = 1/48 (2%) Frame = +1 Query: 109 KSIFHKINRTVAAMEV-DRIASDSENVAMDEKPVVEQGAGDAATSNPE 249 K + K R VA EV DR A++ + ++K V+E G +ATSN + Sbjct: 264 KEVERKAAREVAEKEVADRKAAEQK--LAEQKAVIESVTGSSATSNAQ 309 >At2g39930.1 68415.m04907 isoamylase, putative / starch debranching enzyme, putative similar to isoamylase from [Solanum tuberosum] GI:27728145, [Triticum aestivum] GI:17932898, [Hordeum vulgare] GI:21314275, [Oryza sativa] GI:3252794; contains Pfam profiles PF00128: Alpha amylase catalytic domain, PF02922: Isoamylase N-terminal domain Length = 783 Score = 27.9 bits (59), Expect = 6.3 Identities = 17/55 (30%), Positives = 31/55 (56%) Frame = +1 Query: 43 IHHTKVFIERYNRVHSFCLTVLKSIFHKINRTVAAMEVDRIASDSENVAMDEKPV 207 +HHTK+ N H+F + F + R ++ DR ++++EN+A+ EKP+ Sbjct: 11 LHHTKLNTLFSN--HTFP-KISAPNFKPLFRPISISAKDRRSNEAENIAVVEKPL 62 >At1g61030.1 68414.m06871 expressed protein Length = 840 Score = 27.9 bits (59), Expect = 6.3 Identities = 14/32 (43%), Positives = 18/32 (56%) Frame = -1 Query: 251 RSGLDVAASPAPCSTTGFSSIATFSESEAMRS 156 + G S CSTTGFSSI + S S+ +S Sbjct: 419 KKGFVTIISSDTCSTTGFSSIKSLSVSKRNQS 450 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,535,165 Number of Sequences: 28952 Number of extensions: 274145 Number of successful extensions: 659 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 646 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 659 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1373722560 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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