Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060110.seq
(686 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr 1|||M... 128 1e-30
SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces ... 107 1e-24
SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces p... 107 1e-24
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc... 95 6e-21
SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyce... 69 5e-13
SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein Pss1|Sch... 49 6e-07
SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces pom... 42 1e-04
SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces pombe... 33 0.039
SPAC25B8.04c |||mitochondrial splicing suppressor |Schizosacchar... 29 0.83
SPCC132.04c |||NAD-dependent glutamate dehydrogenase |Schizosacc... 27 2.5
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 26 4.4
SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces pomb... 26 5.9
SPAC823.11 |||sphingosine-1-phosphate phosphatase |Schizosacchar... 26 5.9
SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subuni... 25 7.8
SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr 1... 25 7.8
SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces pombe... 25 7.8
>SPAC22A12.15c |bip1|bip|BiP |Schizosaccharomyces pombe|chr
1|||Manual
Length = 663
Score = 128 bits (308), Expect = 1e-30
Identities = 71/169 (42%), Positives = 98/169 (57%), Gaps = 1/169 (0%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP-TT 268
+GIDLGTTYSCV V ++G+VEIIANDQGNR TPSYVAFT+ ERL+G+AAKNQ P T
Sbjct: 38 IGIDLGTTYSCVAVMKNGRVEIIANDQGNRITPSYVAFTEDERLVGEAAKNQAPSNPENT 97
Query: 269 QYSMPNVSSDVSSKMLLCKPT*STGLSRLSVMEAS*DQGSI*G*RQNLFPRXSQFHGAYE 448
+ + + + + K S ++ + ++ G ++ P +
Sbjct: 98 IFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISAMILSK 157
Query: 449 NEGNCRSLSRQNCAECSYHGSAYFNDSQRQATKDAGTISGLNVLRIINE 595
+ + + AYFND+QRQATKDAGTI+GLNV+RI+NE
Sbjct: 158 MKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKDAGTIAGLNVIRIVNE 206
Score = 70.5 bits (165), Expect = 2e-13
Identities = 39/87 (44%), Positives = 52/87 (59%)
Frame = +3
Query: 372 PKIKVAYKGEDKTFFPXEVSSMVLTKMKETAEAYLGKTVXNAVITGPRTSMTLKDKPQKM 551
P ++V G+ K F P E+S+M+L+KMK+TAEAYLGK V +AV+T P + + K
Sbjct: 132 PLVEVNVGGKKKKFTPEEISAMILSKMKQTAEAYLGKPVTHAVVTVPAYFNDAQRQATKD 191
Query: 552 QVPSQA*TFSESSMKPTAAAIAYGLDK 632
+PTAAAIAYGLDK
Sbjct: 192 AGTIAGLNVIRIVNEPTAAAIAYGLDK 218
Score = 56.8 bits (131), Expect = 3e-09
Identities = 28/65 (43%), Positives = 39/65 (60%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGILRSR*HIRVKTKPFSPXKSVP 435
NP NTIFD KRLIGRKF++ T+ D+K +PF +V+D ++ K K F+P +
Sbjct: 93 NPENTIFDIKRLIGRKFDEKTMAKDIKSFPFHIVNDKNRPLVEVNVGGKKKKFTPEEISA 152
Query: 436 WCLRK 450
L K
Sbjct: 153 MILSK 157
>SPAC13G7.02c |ssa1||heat shock protein Ssa1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 644
Score = 107 bits (257), Expect = 1e-24
Identities = 49/58 (84%), Positives = 53/58 (91%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 77.0 bits (181), Expect = 2e-15
Identities = 47/101 (46%), Positives = 59/101 (58%)
Frame = +3
Query: 372 PKIKVAYKGEDKTFFPXEVSSMVLTKMKETAEAYLGKTVXNAVITGPRTSMTLKDKPQKM 551
P I+V +KGE KTF P E+SSMVL KM+E+AEA+LG V +AV+T P + + K
Sbjct: 99 PLIQVEFKGETKTFTPEEISSMVLLKMRESAEAFLGGKVTDAVVTVPAYFNDSQRQATKD 158
Query: 552 QVPSQA*TFSESSMKPTAAAIAYGLDKKGYLENGNVLXFGL 674
+PTAAAIAYGLD+ E NVL F L
Sbjct: 159 AGLIAGLNVLRIINEPTAAAIAYGLDRSNQHET-NVLIFDL 198
Score = 71.7 bits (168), Expect = 9e-14
Identities = 34/68 (50%), Positives = 45/68 (66%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGILRSR*HIRVKTKPFSPXKSVP 435
NP+NTIFDAKRLIGR+F D VQ+DMKHWPF+V+ G + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRRFNDPEVQSDMKHWPFKVIEKDGKPLIQVEFKGETKTFTPEEISS 119
Query: 436 WCLRK*RK 459
L K R+
Sbjct: 120 MVLLKMRE 127
>SPCC1739.13 |ssa2||heat shock protein Ssa2|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 647
Score = 107 bits (257), Expect = 1e-24
Identities = 49/58 (84%), Positives = 53/58 (91%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
++GIDLGTTYSCVG F + +VEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA P
Sbjct: 4 SIGIDLGTTYSCVGHFSNNRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNP 61
Score = 81.0 bits (191), Expect = 1e-16
Identities = 49/101 (48%), Positives = 60/101 (59%)
Frame = +3
Query: 372 PKIKVAYKGEDKTFFPXEVSSMVLTKMKETAEAYLGKTVXNAVITGPRTSMTLKDKPQKM 551
P ++V YKGE KTF P E+SSMVL KM+ETAEAYLG V +AV+T P + + K
Sbjct: 99 PVLQVEYKGETKTFTPEEISSMVLMKMRETAEAYLGGKVTDAVVTVPAYFNDSQRQATKD 158
Query: 552 QVPSQA*TFSESSMKPTAAAIAYGLDKKGYLENGNVLXFGL 674
+PTAAAIAYGLD+ E+ NVL F L
Sbjct: 159 AGLIAGLNVLRIINEPTAAAIAYGLDRSNQGES-NVLIFDL 198
Score = 74.9 bits (176), Expect = 1e-14
Identities = 36/68 (52%), Positives = 47/68 (69%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGILRSR*HIRVKTKPFSPXKSVP 435
NP+NTIFDAKRLIGRKF+D VQ+DMKHWPF+V+S G + + +TK F+P +
Sbjct: 60 NPHNTIFDAKRLIGRKFDDPEVQSDMKHWPFKVISKDGKPVLQVEYKGETKTFTPEEISS 119
Query: 436 WCLRK*RK 459
L K R+
Sbjct: 120 MVLMKMRE 127
>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 95.5 bits (227), Expect = 6e-21
Identities = 43/58 (74%), Positives = 49/58 (84%)
Frame = +2
Query: 89 AVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTP 262
A+GIDLGTTYSCV V++ VEII NDQG RTTPS+VAFT+TERL+GDAAKNQ A P
Sbjct: 8 AIGIDLGTTYSCVAVWETANVEIIPNDQGARTTPSFVAFTETERLVGDAAKNQAAMNP 65
Score = 67.3 bits (157), Expect = 2e-12
Identities = 46/101 (45%), Positives = 53/101 (52%)
Frame = +3
Query: 372 PKIKVAYKGEDKTFFPXEVSSMVLTKMKETAEAYLGKTVXNAVITGPRTSMTLKDKPQKM 551
P I+V Y GE K F E+S+MVLTKMKE +EA L K V AVIT P + K
Sbjct: 103 PTIEVNYLGEKKQFTAQEISAMVLTKMKEISEAKLNKRVEKAVITVPAYFSDSQRAATKD 162
Query: 552 QVPSQA*TFSESSMKPTAAAIAYGLDKKGYLENGNVLXFGL 674
+PTAAAIAYGLD K + NVL F L
Sbjct: 163 AGAIAGLNVLRIINEPTAAAIAYGLDAKSD-KPKNVLIFDL 202
Score = 65.7 bits (153), Expect = 6e-12
Identities = 25/39 (64%), Positives = 32/39 (82%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVVSDGGI 372
NP NT+FDAKRLIGR++ED Q D+KHWPF+V+ + GI
Sbjct: 64 NPRNTVFDAKRLIGRRYEDPETQKDIKHWPFKVIDNNGI 102
>SPAC664.11 |ssc1|ssp1|Hsp70 chaperone mtHsp70|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 674
Score = 69.3 bits (162), Expect = 5e-13
Identities = 33/66 (50%), Positives = 43/66 (65%), Gaps = 1/66 (1%)
Frame = +2
Query: 80 KAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFT-DTERLIGDAAKNQVAR 256
K P +GIDLGTT SC+ + + ++IAN +G RTTPS VAFT D ERL+G +AK Q
Sbjct: 49 KGPVIGIDLGTTTSCLAIMEGQTPKVIANAEGTRTTPSVVAFTKDGERLVGVSAKRQAVI 108
Query: 257 TPTTQY 274
P +
Sbjct: 109 NPENTF 114
Score = 60.9 bits (141), Expect = 2e-10
Identities = 32/77 (41%), Positives = 43/77 (55%)
Frame = +3
Query: 405 KTFFPXEVSSMVLTKMKETAEAYLGKTVXNAVITGPRTSMTLKDKPQKMQVPSQA*TFSE 584
KT+ P ++ +L+KM+ETA YLGK V NAV+T P + + K
Sbjct: 156 KTYSPSQIGGFILSKMRETASTYLGKDVKNAVVTVPAYFNDSQRQATKAAGAIAGLNVLR 215
Query: 585 SSMKPTAAAIAYGLDKK 635
+PTAAA+AYGLDKK
Sbjct: 216 VVNEPTAAALAYGLDKK 232
Score = 52.8 bits (121), Expect = 4e-08
Identities = 23/28 (82%), Positives = 26/28 (92%)
Frame = +2
Query: 512 AYFNDSQRQATKDAGTISGLNVLRIINE 595
AYFNDSQRQATK AG I+GLNVLR++NE
Sbjct: 192 AYFNDSQRQATKAAGAIAGLNVLRVVNE 219
Score = 44.8 bits (101), Expect = 1e-05
Identities = 18/34 (52%), Positives = 25/34 (73%)
Frame = +1
Query: 256 NPNNTIFDAKRLIGRKFEDATVQADMKHWPFEVV 357
NP NT F KRLIGR+F++ VQ D+K P+++V
Sbjct: 109 NPENTFFATKRLIGRRFKEPEVQRDIKEVPYKIV 142
>SPAC110.04c |pss1|ssp1, SPAP14E8.01c|heat shock protein
Pss1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 49.2 bits (112), Expect = 6e-07
Identities = 20/59 (33%), Positives = 38/59 (64%)
Frame = +2
Query: 77 AKAPAVGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVA 253
++ VGID G + + + V ++ +++I N+ NR+TPS V++ + R +G+AAK+ A
Sbjct: 3 SRTNVVGIDFGNSKTVIAVARNRAIDVIVNEVSNRSTPSLVSYGERSRFLGEAAKSAEA 61
Score = 33.5 bits (73), Expect = 0.029
Identities = 14/28 (50%), Positives = 21/28 (75%)
Frame = +2
Query: 512 AYFNDSQRQATKDAGTISGLNVLRIINE 595
A+F D QR+A +A I+GLN LR++N+
Sbjct: 150 AWFTDIQRRALLEAANIAGLNPLRLMND 177
>SPAC57A7.12 |||heat shock protein Pdr13 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 566
Score = 41.5 bits (93), Expect = 1e-04
Identities = 17/55 (30%), Positives = 31/55 (56%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAR 256
+GI G S + + GK +++AN++GNR PS +++ + G A+ Q+ R
Sbjct: 27 IGISFGNQNSSIAFNRDGKTDVLANEEGNRQIPSILSYHGDQEYHGVQARGQLVR 81
Score = 29.9 bits (64), Expect = 0.36
Identities = 13/34 (38%), Positives = 21/34 (61%)
Frame = +2
Query: 494 CSYHGSAYFNDSQRQATKDAGTISGLNVLRIINE 595
C YF D+QR+A + A +GL VL++I++
Sbjct: 166 CVMSVPVYFTDAQRKALESAANEAGLPVLQLIHD 199
>SPAC1F5.06 |||heat shock protein Lhs1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 848
Score = 33.1 bits (72), Expect = 0.039
Identities = 22/67 (32%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
Frame = +2
Query: 92 VGIDLGTTYSCVGVFQHG-KVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVARTPTT 268
+ ID GT ++ + + G +EI+ R S VAF ER+ G A N R P
Sbjct: 25 LAIDYGTEWTKAALIKPGIPLEIVLTKDTRRKEQSAVAFKGNERIFGVDASNLATRFPA- 83
Query: 269 QYSMPNV 289
+S+ NV
Sbjct: 84 -HSIRNV 89
>SPAC25B8.04c |||mitochondrial splicing suppressor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 378
Score = 28.7 bits (61), Expect = 0.83
Identities = 12/32 (37%), Positives = 18/32 (56%)
Frame = +3
Query: 84 HPQ*ESIWVPRTLALVSSSTGRWRSSPTTRAT 179
HP S W+P LVS+ W +SP+++ T
Sbjct: 283 HPLYSSSWIPTLHDLVSTRCSVWLTSPSSQRT 314
>SPCC132.04c |||NAD-dependent glutamate dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1106
Score = 27.1 bits (57), Expect = 2.5
Identities = 16/54 (29%), Positives = 24/54 (44%)
Frame = +1
Query: 274 FDAKRLIGRKFEDATVQADMKHWPFEVVSDGGILRSR*HIRVKTKPFSPXKSVP 435
FDA +L + ++K E+V +G I R+ H+R K F P P
Sbjct: 830 FDAGKLSPEGYRVLVKDTNLKLPNGEIVRNGTIFRNTAHLRYKADTFVPCGGRP 883
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 26.2 bits (55), Expect = 4.4
Identities = 14/34 (41%), Positives = 18/34 (52%), Gaps = 1/34 (2%)
Frame = +2
Query: 524 DSQRQATK-DAGTISGLNVLRIINETHCCCDCLR 622
+ ++QATK G I G N L+ E HC C R
Sbjct: 117 NDEKQATKMPCGHIFGKNCLQKWLENHCTCPLCR 150
>SPAC3H8.05c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1073
Score = 25.8 bits (54), Expect = 5.9
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = -2
Query: 448 FVSTMELTSSGKKVLSSPLYATLILGCLH 362
F S + L ++ K++L TLI+GC H
Sbjct: 747 FSSKLNLAATPKRLLVDKYTNTLIIGCCH 775
>SPAC823.11 |||sphingosine-1-phosphate phosphatase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 411
Score = 25.8 bits (54), Expect = 5.9
Identities = 16/53 (30%), Positives = 26/53 (49%)
Frame = -1
Query: 170 GRWR*SPPSRAGRHQRKSTWYPDRFLLRVLLPFCIFNQSCYLFLKQLSKRARL 12
G W SP S + H ++++ +F +RVL C+ L K +K+A L
Sbjct: 276 GTWFASPESLSHLHDNLNSYFLLKFFVRVLFGVCMI-----LIWKSFAKQALL 323
>SPBC25H2.13c |cdc20|pol2|DNA polymerase epsilon catalytic subunit a
Pol2 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 2199
Score = 25.4 bits (53), Expect = 7.8
Identities = 14/30 (46%), Positives = 15/30 (50%)
Frame = +2
Query: 437 GAYENEGNCRSLSRQNCAECSYHGSAYFND 526
GAY N C +S N A CS SA ND
Sbjct: 1689 GAYSNA--CVDISLSNLALCSILNSALIND 1716
>SPAPJ760.02c |app1||App1 protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 857
Score = 25.4 bits (53), Expect = 7.8
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = +2
Query: 200 AFTDTERLIGDAAKNQVARTPTTQYSMPNVSSDV 301
AF D + G AK A P+T+ + N+S V
Sbjct: 155 AFYDASQTFGSTAKVAPAPAPSTKTPLANISKPV 188
>SPAC3G9.11c |||pyruvate decarboxylase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 570
Score = 25.4 bits (53), Expect = 7.8
Identities = 17/51 (33%), Positives = 28/51 (54%), Gaps = 1/51 (1%)
Frame = -2
Query: 262 WGSRHLVLGGISDETLGVCERNIRRSGPVALVVGD-DLHLPVLEDTNARVR 113
WGS +G + TLG+ + + + L+VGD LHL V ++ +A +R
Sbjct: 418 WGSIGWSVGAMFGATLGIKDSDAPHRRSI-LIVGDGSLHLTV-QEISATIR 466
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,995,239
Number of Sequences: 5004
Number of extensions: 64216
Number of successful extensions: 214
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 187
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 317927284
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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