Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= NV060109.seq
(699 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 182 4e-48
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 182 4e-48
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 174 8e-46
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 173 1e-45
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.2
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 2.8
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 2.8
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 3.7
DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein. 21 8.5
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 182 bits (442), Expect = 4e-48
Identities = 91/122 (74%), Positives = 97/122 (79%)
Frame = +3
Query: 261 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 440
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 13 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 72
Query: 441 NGQTREHALLAFTLGVKQLIVGVTKWIPLNHHTVSPDLRKSRRXYPHTSRRLATTPAAVA 620
NGQTREHALLAFTLGVKQLIVGV K + ++ +++ PAAVA
Sbjct: 73 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 132
Query: 621 FV 626
FV
Sbjct: 133 FV 134
Score = 75.8 bits (178), Expect = 4e-16
Identities = 36/62 (58%), Positives = 39/62 (62%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEXXXXXXXXXXXXGYNPSCCRFRGPFSGRHGDNMLELXXKMPWV 688
NKMDSTEPPYSE RFEE GYNP+ F P SG HGDNMLE+ KMPW
Sbjct: 96 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFV-PISGWHGDNMLEVSSKMPWF 154
Query: 689 QG 694
+G
Sbjct: 155 KG 156
Score = 23.4 bits (48), Expect = 2.1
Identities = 9/9 (100%), Positives = 9/9 (100%)
Frame = +2
Query: 227 WVLDKLKAE 253
WVLDKLKAE
Sbjct: 1 WVLDKLKAE 9
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 182 bits (442), Expect = 4e-48
Identities = 91/122 (74%), Positives = 97/122 (79%)
Frame = +3
Query: 261 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 440
GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK
Sbjct: 70 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 129
Query: 441 NGQTREHALLAFTLGVKQLIVGVTKWIPLNHHTVSPDLRKSRRXYPHTSRRLATTPAAVA 620
NGQTREHALLAFTLGVKQLIVGV K + ++ +++ PAAVA
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVA 189
Query: 621 FV 626
FV
Sbjct: 190 FV 191
Score = 141 bits (342), Expect = 5e-36
Identities = 65/66 (98%), Positives = 65/66 (98%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 75.8 bits (178), Expect = 4e-16
Identities = 36/62 (58%), Positives = 39/62 (62%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEXXXXXXXXXXXXGYNPSCCRFRGPFSGRHGDNMLELXXKMPWV 688
NKMDSTEPPYSE RFEE GYNP+ F P SG HGDNMLE+ KMPW
Sbjct: 153 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFV-PISGWHGDNMLEVSSKMPWF 211
Query: 689 QG 694
+G
Sbjct: 212 KG 213
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 174 bits (423), Expect = 8e-46
Identities = 87/118 (73%), Positives = 93/118 (78%)
Frame = +3
Query: 273 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 452
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 453 REHALLAFTLGVKQLIVGVTKWIPLNHHTVSPDLRKSRRXYPHTSRRLATTPAAVAFV 626
REHALLAFTLGVKQLIVGV K + ++ +++ PAAVAFV
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFV 118
Score = 75.8 bits (178), Expect = 4e-16
Identities = 36/62 (58%), Positives = 39/62 (62%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEXXXXXXXXXXXXGYNPSCCRFRGPFSGRHGDNMLELXXKMPWV 688
NKMDSTEPPYSE RFEE GYNP+ F P SG HGDNMLE+ KMPW
Sbjct: 80 NKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFV-PISGWHGDNMLEVSSKMPWF 138
Query: 689 QG 694
+G
Sbjct: 139 KG 140
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 173 bits (422), Expect = 1e-45
Identities = 87/122 (71%), Positives = 96/122 (78%)
Frame = +3
Query: 261 GITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISK 440
GITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG GEFEAGISK
Sbjct: 70 GITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISK 129
Query: 441 NGQTREHALLAFTLGVKQLIVGVTKWIPLNHHTVSPDLRKSRRXYPHTSRRLATTPAAVA 620
NGQTREHALLAFTLGVKQLIVGV K + + ++ +++ A+VA
Sbjct: 130 NGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVA 189
Query: 621 FV 626
FV
Sbjct: 190 FV 191
Score = 141 bits (342), Expect = 5e-36
Identities = 65/66 (98%), Positives = 65/66 (98%)
Frame = +2
Query: 56 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 235
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 236 DKLKAE 253
DKLKAE
Sbjct: 61 DKLKAE 66
Score = 64.9 bits (151), Expect = 7e-13
Identities = 32/62 (51%), Positives = 35/62 (56%)
Frame = +2
Query: 509 NKMDSTEPPYSEPRFEEXXXXXXXXXXXXGYNPSCCRFRGPFSGRHGDNMLELXXKMPWV 688
NKMD T+PPYSE RFEE GYN + F P SG HGDNMLE K PW
Sbjct: 153 NKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFV-PISGWHGDNMLEPSPKTPWY 211
Query: 689 QG 694
+G
Sbjct: 212 KG 213
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +3
Query: 312 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 416
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.52
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 68 KTHINIVVIGHVDSGKST 121
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.2
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 77 INIVVIGHVDSGKST 121
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 2.8
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 69 FSLPIFG*SRITNCV*Y 19
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 2.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +3
Query: 297 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 398
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 3.7
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +3
Query: 297 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 398
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
>DQ071552-1|AAY82248.1| 495|Apis mellifera anarchy 1 protein.
Length = 495
Score = 21.4 bits (43), Expect = 8.5
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = -2
Query: 311 IVLASFELPESNIDCDTXSAQPLVCPIPKHI*RILY 204
IV+ FE+ +S D A IP ++ R+L+
Sbjct: 54 IVIGGFEIEKSEDDSFNNQADKSEKRIPLYVCRVLH 89
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 189,971
Number of Sequences: 438
Number of extensions: 3690
Number of successful extensions: 29
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 17
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 25
length of database: 146,343
effective HSP length: 56
effective length of database: 121,815
effective search space used: 21439440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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