BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060108.seq (677 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe... 62 7e-11 SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr 2|... 26 5.8 SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosacch... 26 5.8 SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizos... 26 5.8 >SPAC1687.05 |pli1||SUMO E3 ligase Pli1|Schizosaccharomyces pombe|chr 1|||Manual Length = 727 Score = 62.1 bits (144), Expect = 7e-11 Identities = 26/66 (39%), Positives = 39/66 (59%), Gaps = 2/66 (3%) Frame = +2 Query: 473 VSVPARQLPAP--ACFDASLFLQMNERKPTWLCPVCDRAAPYDSLVVDGYFQEVLTSXAP 646 +S+P R + CFDAS FL+MN++ P+W+CPVC + L++DG+ Q +L S Sbjct: 312 ISLPVRSVFCKHIQCFDASAFLEMNKQTPSWMCPVCASHIQFSDLIIDGFMQHILESTPS 371 Query: 647 GRPTAT 664 T T Sbjct: 372 NSETIT 377 Score = 47.2 bits (107), Expect = 2e-06 Identities = 24/80 (30%), Positives = 45/80 (56%) Frame = +3 Query: 267 TRAYVLSVFMVKKLTSAELLQRLKNKGTKNPDYTRSLIKEKLSEDYDSEIATTSLRVSLM 446 T++Y + V VK T L+ ++K++ ++ + +I+ +++ D++I TS +SL Sbjct: 248 TKSYSVVVCFVKVYTIENLVDQIKSRKAESKE---KIIERIKNDNQDADIIATSTDISLK 304 Query: 447 CPLGKMRMSCPCRPANCPHL 506 CPL R+S P R C H+ Sbjct: 305 CPLSFSRISLPVRSVFCKHI 324 >SPBC14C8.07c |cdc18||MCM loader|Schizosaccharomyces pombe|chr 2|||Manual Length = 577 Score = 25.8 bits (54), Expect = 5.8 Identities = 26/98 (26%), Positives = 38/98 (38%), Gaps = 4/98 (4%) Frame = +3 Query: 174 KKATASSKHIVAREAVAHGLQHDPGDVAADFTRAYVLSVFMVKKLTSAELLQRLKNKGTK 353 +KA +H + QHD + D RA + V S RLKN G + Sbjct: 420 RKALDICRHAIELAEREWKAQHDNTLSSVDIPRASIAHVVRATSAMSQSASARLKNLGLQ 479 Query: 354 NPDYTRSLI---KEKLS-EDYDSEIATTSLRVSLMCPL 455 +L+ K LS D + ++ LR L+ PL Sbjct: 480 QKAILCTLVVCEKTSLSVADVFEKYSSLCLRDRLIYPL 517 >SPBC543.08 |||phosphoinositide biosynthesis protein |Schizosaccharomyces pombe|chr 2|||Manual Length = 250 Score = 25.8 bits (54), Expect = 5.8 Identities = 12/34 (35%), Positives = 17/34 (50%) Frame = -3 Query: 174 WFWSWLIGRYWIW*RTHLVIDFDINARREVVLLF 73 WFW+ L+ Y W + IDF +R V L+ Sbjct: 69 WFWTSLVYFYHAWDQKRNKIDFKFISRYIVATLW 102 >SPBC1539.05 |cog3||Golgi transport complex subunit Cog3 |Schizosaccharomyces pombe|chr 2|||Manual Length = 735 Score = 25.8 bits (54), Expect = 5.8 Identities = 11/39 (28%), Positives = 22/39 (56%) Frame = +2 Query: 512 FDASLFLQMNERKPTWLCPVCDRAAPYDSLVVDGYFQEV 628 F+ S L ++E+ T LC VC + + +++G ++V Sbjct: 379 FENSRSLILHEKNLTELCEVCSYIQSFQNAILEGEREDV 417 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,282,324 Number of Sequences: 5004 Number of extensions: 38684 Number of successful extensions: 111 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 108 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 111 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 311890690 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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