Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= NV060106.seq
         (685 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch...   132   6e-32
SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|ch...    28   1.1  

>SPBC19C7.06 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 716

 Score =  132 bits (318), Expect = 6e-32
 Identities = 57/83 (68%), Positives = 68/83 (81%)
 Frame = +3

Query: 12  EFRKLGVKDGYFPIFVSNAALEREKTHIADFAPEVAWVTHSGXSELAEPIAVRPTSXXVM 191
           E +KLGV++GYFP+FVS+  LE+EK H+  FAPEVAWVT +G SEL EPIA+RPTS  VM
Sbjct: 258 EIKKLGVRNGYFPLFVSSKVLEKEKDHVEGFAPEVAWVTRAGTSELDEPIAIRPTSETVM 317

Query: 192 YPAYAKWIQSHRDLXLKLNXWTT 260
           YP YAKWI+SHRDL LKLN W +
Sbjct: 318 YPYYAKWIRSHRDLPLKLNQWNS 340



 Score =  116 bits (278), Expect = 4e-27
 Identities = 53/85 (62%), Positives = 62/85 (72%)
 Frame = +2

Query: 254 DNVVRWEFKQPQPFLXXREFLWQEGHTAXXXKXXXXXXXLIXLDLYARVYEELLAIPVIK 433
           ++VVRWEFK PQPFL  REFLWQEGHTA              LDLYAR+Y +LLA+PVIK
Sbjct: 339 NSVVRWEFKNPQPFLRTREFLWQEGHTAHMTLEGATEEVHQILDLYARIYTDLLAVPVIK 398

Query: 434 GRKTEKEKFAGGDYTTTVEAYVPAS 508
           G K+E EKFAGG +TTTVE Y+P +
Sbjct: 399 GVKSENEKFAGGMFTTTVEGYIPTT 423



 Score = 63.7 bits (148), Expect = 2e-11
 Identities = 37/57 (64%), Positives = 42/57 (73%), Gaps = 7/57 (12%)
 Frame = +1

Query: 511 RXVQGATSHHLGQNFSKMFEIVYDD--AE---TLE--KNYVYQNSWGFTTRTIGVMV 660
           R +QGATSH LGQNFSKMF IV +D  AE   T E  K +V+QNSWG +TRTIGV V
Sbjct: 425 RGIQGATSHCLGQNFSKMFNIVVEDPNAEIGPTGERPKLFVWQNSWGLSTRTIGVAV 481


>SPCC1183.05c |lig4||DNA ligase Lig4|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 923

 Score = 28.3 bits (60), Expect = 1.1
 Identities = 16/57 (28%), Positives = 29/57 (50%)
 Frame = -2

Query: 651 TNSSSCEAPRILIDVILLQSFGIVVYNLEHLRKVLT*MMTRRSLNASALAGT*ASTV 481
           ++ SS     ++ D++ L    +V Y+LE  R++L  ++ R S   S L     ST+
Sbjct: 380 SSHSSYSPYYVVFDILYLNGKSLVKYSLESRRRILEKVIVRESHRMSILPYKVGSTI 436


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,178,673
Number of Sequences: 5004
Number of extensions: 35819
Number of successful extensions: 92
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 86
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 315915086
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)