BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= NV060106.seq (685 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S... 120 7e-28 At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S... 120 7e-28 At5g52520.1 68418.m06516 tRNA synthetase class II (G, H, P and S... 78 5e-15 At5g45990.1 68418.m05656 crooked neck protein, putative / cell c... 27 8.8 At4g15850.1 68417.m02410 DEAD/DEAH box helicase, putative simila... 27 8.8 >At3g62120.2 68416.m06980 tRNA synthetase class II (G, H, P and S) family protein similar to SP|P07814 Bifunctional aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA ligase)] {Homo sapiens}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon binding domain Length = 530 Score = 120 bits (290), Expect = 7e-28 Identities = 54/82 (65%), Positives = 63/82 (76%) Frame = +3 Query: 9 AEFRKLGVKDGYFPIFVSNAALEREKTHIADFAPEVAWVTHSGXSELAEPIAVRPTSXXV 188 AE +K+ VK+ YFP+FVS LE+EK HI FAPEVAWVT SG S+L PIA+RPTS V Sbjct: 99 AEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSETV 158 Query: 189 MYPAYAKWIQSHRDLXLKLNXW 254 MYP Y+KWI+ HRDL LKLN W Sbjct: 159 MYPYYSKWIRGHRDLPLKLNQW 180 Score = 115 bits (277), Expect = 3e-26 Identities = 51/82 (62%), Positives = 60/82 (73%) Frame = +2 Query: 257 NVVRWEFKQPQPFLXXREFLWQEGHTAXXXKXXXXXXXLIXLDLYARVYEELLAIPVIKG 436 NVVRWEF P PF+ REFLWQEGHTA K L L+LY R+YEE LA+PV+KG Sbjct: 182 NVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKG 241 Query: 437 RKTEKEKFAGGDYTTTVEAYVP 502 K+E EKFAGG YTT+VEA++P Sbjct: 242 MKSENEKFAGGLYTTSVEAFIP 263 Score = 68.9 bits (161), Expect = 3e-12 Identities = 32/50 (64%), Positives = 40/50 (80%) Frame = +1 Query: 511 RXVQGATSHHLGQNFSKMFEIVYDDAETLEKNYVYQNSWGFTTRTIGVMV 660 R VQGATSH LGQNF+KMFEI +++ E E V+QNSW ++TRTIGVM+ Sbjct: 267 RGVQGATSHCLGQNFAKMFEINFEN-EKAETEMVWQNSWAYSTRTIGVMI 315 >At3g62120.1 68416.m06979 tRNA synthetase class II (G, H, P and S) family protein similar to SP|P07814 Bifunctional aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA ligase)] {Homo sapiens}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon binding domain Length = 530 Score = 120 bits (290), Expect = 7e-28 Identities = 54/82 (65%), Positives = 63/82 (76%) Frame = +3 Query: 9 AEFRKLGVKDGYFPIFVSNAALEREKTHIADFAPEVAWVTHSGXSELAEPIAVRPTSXXV 188 AE +K+ VK+ YFP+FVS LE+EK HI FAPEVAWVT SG S+L PIA+RPTS V Sbjct: 99 AEIKKMKVKNCYFPLFVSPGVLEKEKDHIEGFAPEVAWVTKSGKSDLEVPIAIRPTSETV 158 Query: 189 MYPAYAKWIQSHRDLXLKLNXW 254 MYP Y+KWI+ HRDL LKLN W Sbjct: 159 MYPYYSKWIRGHRDLPLKLNQW 180 Score = 115 bits (277), Expect = 3e-26 Identities = 51/82 (62%), Positives = 60/82 (73%) Frame = +2 Query: 257 NVVRWEFKQPQPFLXXREFLWQEGHTAXXXKXXXXXXXLIXLDLYARVYEELLAIPVIKG 436 NVVRWEF P PF+ REFLWQEGHTA K L L+LY R+YEE LA+PV+KG Sbjct: 182 NVVRWEFSNPTPFIRSREFLWQEGHTAFATKAEADEEVLQILELYRRIYEEYLAVPVVKG 241 Query: 437 RKTEKEKFAGGDYTTTVEAYVP 502 K+E EKFAGG YTT+VEA++P Sbjct: 242 MKSENEKFAGGLYTTSVEAFIP 263 Score = 68.9 bits (161), Expect = 3e-12 Identities = 32/50 (64%), Positives = 40/50 (80%) Frame = +1 Query: 511 RXVQGATSHHLGQNFSKMFEIVYDDAETLEKNYVYQNSWGFTTRTIGVMV 660 R VQGATSH LGQNF+KMFEI +++ E E V+QNSW ++TRTIGVM+ Sbjct: 267 RGVQGATSHCLGQNFAKMFEINFEN-EKAETEMVWQNSWAYSTRTIGVMI 315 >At5g52520.1 68418.m06516 tRNA synthetase class II (G, H, P and S) family protein similar to SP|P07814 Bifunctional aminoacyl-tRNA synthetase [Includes: Glutamyl-tRNA synthetase (EC 6.1.1.17) (Glutamate--tRNA ligase); Prolyl-tRNA synthetase (EC 6.1.1.15) (Proline--tRNA ligase)] {Homo sapiens}; contains Pfam profiles PF00587: tRNA synthetase class II core domain (G, H, P, S and T), PF03129: Anticodon binding domain Length = 543 Score = 78.2 bits (184), Expect = 5e-15 Identities = 33/81 (40%), Positives = 52/81 (64%) Frame = +3 Query: 12 EFRKLGVKDGYFPIFVSNAALEREKTHIADFAPEVAWVTHSGXSELAEPIAVRPTSXXVM 191 +F++ G + YFP F+ + +E+E +H+ F+PE+A VT G EL E + VRPTS ++ Sbjct: 116 KFKETGHSNMYFPQFIPYSFIEKEASHVEGFSPELALVTVGGGKELEEKLVVRPTSETIV 175 Query: 192 YPAYAKWIQSHRDLXLKLNXW 254 + +WI S+RDL L +N W Sbjct: 176 NHMFTQWIHSYRDLPLMINQW 196 Score = 73.3 bits (172), Expect = 1e-13 Identities = 37/79 (46%), Positives = 46/79 (58%) Frame = +2 Query: 257 NVVRWEFKQPQPFLXXREFLWQEGHTAXXXKXXXXXXXLIXLDLYARVYEELLAIPVIKG 436 NV RWE + +PF+ EFLWQEGHTA +++Y R E AIPVI G Sbjct: 198 NVTRWEMRT-KPFIRTLEFLWQEGHTAHATPEEAEKEAKQMIEIYTRFAFEQTAIPVIPG 256 Query: 437 RKTEKEKFAGGDYTTTVEA 493 RK++ E FAG D T T+EA Sbjct: 257 RKSKLETFAGADITYTIEA 275 Score = 52.0 bits (119), Expect = 4e-07 Identities = 23/53 (43%), Positives = 35/53 (66%) Frame = +1 Query: 502 GQRRXVQGATSHHLGQNFSKMFEIVYDDAETLEKNYVYQNSWGFTTRTIGVMV 660 G R+ +Q TSH+LGQNFS+ F + D E E+ +V+Q SW +TR +G ++ Sbjct: 278 GDRKALQAGTSHNLGQNFSRAFGTQFAD-ENGERQHVWQTSWAVSTRFVGGII 329 >At5g45990.1 68418.m05656 crooked neck protein, putative / cell cycle protein, putative similar to Swiss-Prot:P17886 crooked neck protein [Drosophila melanogaster] Length = 673 Score = 27.5 bits (58), Expect = 8.8 Identities = 11/31 (35%), Positives = 15/31 (48%) Frame = +3 Query: 201 YAKWIQSHRDLXLKLNXWTTSLDGSSNNRNL 293 YAKW +S D + W +L+G N L Sbjct: 84 YAKWEESQMDYARARSVWERALEGEYRNHTL 114 >At4g15850.1 68417.m02410 DEAD/DEAH box helicase, putative similar to D-E-A-D box protein [Drosophila melanogaster] GI:499204; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 512 Score = 27.5 bits (58), Expect = 8.8 Identities = 14/39 (35%), Positives = 20/39 (51%) Frame = +1 Query: 472 LHHHGRSLRTGQRRXVQGATSHHLGQNFSKMFEIVYDDA 588 +H GR+ R GQ S+H + FSK+ E V D+ Sbjct: 413 IHRAGRTARAGQAGRCFTLLSNHEVRRFSKLLEKVGSDS 451 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 11,586,424 Number of Sequences: 28952 Number of extensions: 192473 Number of successful extensions: 476 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 455 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 473 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1447936096 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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